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Protein

Alpha-1,3-mannosyltransferase MNN1

Gene

MNN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for addition of the terminal mannose residues to the outer chain of core N-linked polysaccharides and to O-linked mannotriose. Implicated in late Golgi modifications.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,3-mannosyltransferase activity Source: SGD

GO - Biological processi

  • N-glycan processing Source: SGD
  • protein O-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YER001W-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT71. Glycosyltransferase Family 71.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyltransferase MNN1 (EC:2.4.1.-)
Gene namesi
Name:MNN1
Ordered Locus Names:YER001W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER001W.
SGDiS000000803. MNN1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence analysisAdd
BLAST
Transmembranei17 – 3317Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini34 – 762729LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 762762Alpha-1,3-mannosyltransferase MNN1PRO_0000080558Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence analysis
Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence analysis
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence analysis
Glycosylationi383 – 3831N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP39106.

PTM databases

SwissPalmiP39106.

Interactioni

Protein-protein interaction databases

BioGridi36731. 26 interactions.
DIPiDIP-7589N.
IntActiP39106. 3 interactions.
MINTiMINT-1361711.

Structurei

3D structure databases

ProteinModelPortaliP39106.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MNN1/MNT family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003622.
HOGENOMiHOG000113616.
InParanoidiP39106.
KOiK05538.
OMAiNERCIAR.
OrthoDBiEOG092C3H4R.

Family and domain databases

InterProiIPR022751. Alpha_mannosyltransferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF11051. Mannosyl_trans3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

P39106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLALRRFILN QRSLRSCTIP ILVGALIIIL VLFQLVTHRN DALIRSSNVN
60 70 80 90 100
STNKKTLKDA DPKVLIEAFG SPEVDPVDTI PVSPLELVPF YDQSIDTKRS
110 120 130 140 150
SSWLINKKGY YKHFNELSLT DRCKFYFRTL YTLDDEWTNS VKKLEYSIND
160 170 180 190 200
NEGVDEGKDA NGNPMDEKSE RLYRRKYDMF QAFERIRAYD RCFMQANPVN
210 220 230 240 250
IQEIFPKSDK MSKERVQSKL IKTLNATFPN YDPDNFKKYD QFEFEHKMFP
260 270 280 290 300
FINNFTTETF HEMVPKITSP FGKVLEQGFL PKFDHKTGKV QEYFKYEYDP
310 320 330 340 350
SKTFWANWRD MSAKVAGRGI VLSLGSNQFP LAVKFIASLR FEGNTLPIQV
360 370 380 390 400
VYRGDELSQE LVDKLIYAAR SPDFKPVENN YDNSTNVPQE IWFLDVSNTI
410 420 430 440 450
HPKWRGDFGS YKSKWLVVLL NLLQEFVFLD IDAISYEKID NYFKTTEYQK
460 470 480 490 500
TGTVFYRERA LRENVNERCI ARYETLLPRN LESKNFQNSL LIDPDHALNE
510 520 530 540 550
CDNTLTTEEY IFKAFFHHRR QHQLEAGLFA VDKSKHTIPL VLAAMIHLAK
560 570 580 590 600
NTAHCTHGDK ENFWLGFLAA GHTYALQGVY SGAIGDYVKK TDLNGKRQEA
610 620 630 640 650
AVEICSGQIA HMSTDKKTLL WVNGGGTFCK HDNAAKDDWK KDGDFKKFKD
660 670 680 690 700
QFKTFEEMEK YYYITPISSK YVILPDPKSD DWHRASAGAC GGYIWCATHK
710 720 730 740 750
TLLKPYSYNH RTTHGELITL DEEQRLHIDA VNTVWSHANK DNTRSFTEEE
760
IKELENSRHE QS
Length:762
Mass (Da):88,529
Last modified:February 1, 1995 - v1
Checksum:i7EF7967E2C60B185
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti338 – 3381S → T in AAA53676 (PubMed:8146181).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23753 Genomic DNA. Translation: AAA53676.1.
U18778 Genomic DNA. Translation: AAB64534.1.
BK006939 Genomic DNA. Translation: DAA07651.1.
PIRiS50459.
RefSeqiNP_010916.1. NM_001178892.1.

Genome annotation databases

EnsemblFungiiYER001W; YER001W; YER001W.
GeneIDi856718.
KEGGisce:YER001W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23753 Genomic DNA. Translation: AAA53676.1.
U18778 Genomic DNA. Translation: AAB64534.1.
BK006939 Genomic DNA. Translation: DAA07651.1.
PIRiS50459.
RefSeqiNP_010916.1. NM_001178892.1.

3D structure databases

ProteinModelPortaliP39106.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36731. 26 interactions.
DIPiDIP-7589N.
IntActiP39106. 3 interactions.
MINTiMINT-1361711.

Protein family/group databases

CAZyiGT71. Glycosyltransferase Family 71.

PTM databases

SwissPalmiP39106.

Proteomic databases

MaxQBiP39106.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER001W; YER001W; YER001W.
GeneIDi856718.
KEGGisce:YER001W.

Organism-specific databases

EuPathDBiFungiDB:YER001W.
SGDiS000000803. MNN1.

Phylogenomic databases

GeneTreeiENSGT00390000003622.
HOGENOMiHOG000113616.
InParanoidiP39106.
KOiK05538.
OMAiNERCIAR.
OrthoDBiEOG092C3H4R.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:YER001W-MONOMER.

Miscellaneous databases

PROiP39106.

Family and domain databases

InterProiIPR022751. Alpha_mannosyltransferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF11051. Mannosyl_trans3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiMNN1_YEAST
AccessioniPrimary (citable) accession number: P39106
Secondary accession number(s): D3DLP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1780 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.