P39106 (MNN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-mannosyltransferase MNN1
EC=2.4.1.-

Gene names

Name:	MNN1
Ordered Locus Names:	YER001W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for addition of the terminal mannose residues to the outer chain of core N-linked polysaccharides and to O-linked mannotriose. Implicated in late Golgi modifications.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Miscellaneous	Present with 1780 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the MNN1/MNT family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-glycan processing Inferred from mutant phenotype Ref.1. Source: SGD protein O-linked glycosylation Inferred from direct assay. Source: SGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosyltransferase activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	Alpha-1,3-mannosyltransferase MNN1		PRO_0000080558
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 33	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	34 – 762	729	Lumenal Potential
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Glycosylation	225	1	N-linked (GlcNAc...) Potential
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	383	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	338	1	S → T in AAA53676. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39106 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7EF7967E2C60B185	FASTA	762	88,529
	10 20 30 40 50 60 MLALRRFILN QRSLRSCTIP ILVGALIIIL VLFQLVTHRN DALIRSSNVN STNKKTLKDA 70 80 90 100 110 120 DPKVLIEAFG SPEVDPVDTI PVSPLELVPF YDQSIDTKRS SSWLINKKGY YKHFNELSLT 130 140 150 160 170 180 DRCKFYFRTL YTLDDEWTNS VKKLEYSIND NEGVDEGKDA NGNPMDEKSE RLYRRKYDMF 190 200 210 220 230 240 QAFERIRAYD RCFMQANPVN IQEIFPKSDK MSKERVQSKL IKTLNATFPN YDPDNFKKYD 250 260 270 280 290 300 QFEFEHKMFP FINNFTTETF HEMVPKITSP FGKVLEQGFL PKFDHKTGKV QEYFKYEYDP 310 320 330 340 350 360 SKTFWANWRD MSAKVAGRGI VLSLGSNQFP LAVKFIASLR FEGNTLPIQV VYRGDELSQE 370 380 390 400 410 420 LVDKLIYAAR SPDFKPVENN YDNSTNVPQE IWFLDVSNTI HPKWRGDFGS YKSKWLVVLL 430 440 450 460 470 480 NLLQEFVFLD IDAISYEKID NYFKTTEYQK TGTVFYRERA LRENVNERCI ARYETLLPRN 490 500 510 520 530 540 LESKNFQNSL LIDPDHALNE CDNTLTTEEY IFKAFFHHRR QHQLEAGLFA VDKSKHTIPL 550 560 570 580 590 600 VLAAMIHLAK NTAHCTHGDK ENFWLGFLAA GHTYALQGVY SGAIGDYVKK TDLNGKRQEA 610 620 630 640 650 660 AVEICSGQIA HMSTDKKTLL WVNGGGTFCK HDNAAKDDWK KDGDFKKFKD QFKTFEEMEK 670 680 690 700 710 720 YYYITPISSK YVILPDPKSD DWHRASAGAC GGYIWCATHK TLLKPYSYNH RTTHGELITL 730 740 750 760 DEEQRLHIDA VNTVWSHANK DNTRSFTEEE IKELENSRHE QS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins." Yip C.L., Welch S.K., Klebl F., Gilbert T., Seidel P., Grant F.J., O'Hara P.J., Mackay V.L. Proc. Natl. Acad. Sci. U.S.A. 91:2723-2727(1994) [PubMed: 8146181] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26109 / X2180.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23753 Genomic DNA. Translation: AAA53676.1. U18778 Genomic DNA. Translation: AAB64534.1. BK006939 Genomic DNA. Translation: DAA07651.1.
PIR	S50459.
RefSeq	NP_010916.1. NM_001178892.1.
3D structure databases
ProteinModelPortal	P39106.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7589N.
IntAct	P39106. 2 interactions.
MINT	MINT-1361711.
STRING	P39106.
Protein family/group databases
CAZy	GT71. Glycosyltransferase Family 71.
Proteomic databases
PeptideAtlas	P39106.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER001W; YER001W; YER001W.
GeneID	856718.
KEGG	sce:YER001W.
NMPDR	fig|4932.3.peg.1974.
Organism-specific databases
CYGD	YER001w.
SGD	S000000803. MNN1.
Phylogenomic databases
eggNOG	fuNOG14394.
GeneTree	EFGT00050000003823.
OMA	FANKFLA.
OrthoDB	EOG4PVS70.
Gene expression databases
ArrayExpress	P39106.
Genevestigator	P39106.
GermOnline	YER001W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022751. Alpha_mannosyltransferase. [Graphical view]
KO	K05538.
Pfam	PF11051. Mannosyl_trans3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982805.

Entry information

Entry name

MNN1_YEAST

Accession

Primary (citable) accession number: P39106
Secondary accession number(s): D3DLP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program