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Protein

Alpha-1,3-mannosyltransferase MNN1

Gene

MNN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for addition of the terminal mannose residues to the outer chain of core N-linked polysaccharides and to O-linked mannotriose. Implicated in late Golgi modifications.

Miscellaneous

Present with 1780 molecules/cell in log phase SD medium.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,3-mannosyltransferase activity Source: SGD

GO - Biological processi

  • N-glycan processing Source: SGD
  • protein O-linked glycosylation Source: SGD

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YER001W-MONOMER
YEAST:YER001W-MONOMER
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT71 Glycosyltransferase Family 71

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyltransferase MNN1 (EC:2.4.1.-)
Gene namesi
Name:MNN1
Ordered Locus Names:YER001W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER001W
SGDiS000000803 MNN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 33Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini34 – 762LumenalSequence analysisAdd BLAST729

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805581 – 762Alpha-1,3-mannosyltransferase MNN1Add BLAST762

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi225N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi254N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi383N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP39106
PaxDbiP39106
PRIDEiP39106

PTM databases

SwissPalmiP39106

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DPH1P404872EBI-11064,EBI-25162

Protein-protein interaction databases

BioGridi36731, 42 interactors
DIPiDIP-7589N
IntActiP39106, 15 interactors
MINTiP39106
STRINGi4932.YER001W

Structurei

3D structure databases

ProteinModelPortaliP39106
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MNN1/MNT family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003622
HOGENOMiHOG000113616
InParanoidiP39106
KOiK05538
OMAiGHINGED
OrthoDBiEOG092C3H4R

Family and domain databases

InterProiView protein in InterPro
IPR022751 Alpha_mannosyltransferase
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF11051 Mannosyl_trans3, 1 hit
SUPFAMiSSF53448 SSF53448, 2 hits

Sequencei

Sequence statusi: Complete.

P39106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLALRRFILN QRSLRSCTIP ILVGALIIIL VLFQLVTHRN DALIRSSNVN
60 70 80 90 100
STNKKTLKDA DPKVLIEAFG SPEVDPVDTI PVSPLELVPF YDQSIDTKRS
110 120 130 140 150
SSWLINKKGY YKHFNELSLT DRCKFYFRTL YTLDDEWTNS VKKLEYSIND
160 170 180 190 200
NEGVDEGKDA NGNPMDEKSE RLYRRKYDMF QAFERIRAYD RCFMQANPVN
210 220 230 240 250
IQEIFPKSDK MSKERVQSKL IKTLNATFPN YDPDNFKKYD QFEFEHKMFP
260 270 280 290 300
FINNFTTETF HEMVPKITSP FGKVLEQGFL PKFDHKTGKV QEYFKYEYDP
310 320 330 340 350
SKTFWANWRD MSAKVAGRGI VLSLGSNQFP LAVKFIASLR FEGNTLPIQV
360 370 380 390 400
VYRGDELSQE LVDKLIYAAR SPDFKPVENN YDNSTNVPQE IWFLDVSNTI
410 420 430 440 450
HPKWRGDFGS YKSKWLVVLL NLLQEFVFLD IDAISYEKID NYFKTTEYQK
460 470 480 490 500
TGTVFYRERA LRENVNERCI ARYETLLPRN LESKNFQNSL LIDPDHALNE
510 520 530 540 550
CDNTLTTEEY IFKAFFHHRR QHQLEAGLFA VDKSKHTIPL VLAAMIHLAK
560 570 580 590 600
NTAHCTHGDK ENFWLGFLAA GHTYALQGVY SGAIGDYVKK TDLNGKRQEA
610 620 630 640 650
AVEICSGQIA HMSTDKKTLL WVNGGGTFCK HDNAAKDDWK KDGDFKKFKD
660 670 680 690 700
QFKTFEEMEK YYYITPISSK YVILPDPKSD DWHRASAGAC GGYIWCATHK
710 720 730 740 750
TLLKPYSYNH RTTHGELITL DEEQRLHIDA VNTVWSHANK DNTRSFTEEE
760
IKELENSRHE QS
Length:762
Mass (Da):88,529
Last modified:February 1, 1995 - v1
Checksum:i7EF7967E2C60B185
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti338S → T in AAA53676 (PubMed:8146181).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23753 Genomic DNA Translation: AAA53676.1
U18778 Genomic DNA Translation: AAB64534.1
BK006939 Genomic DNA Translation: DAA07651.1
PIRiS50459
RefSeqiNP_010916.1, NM_001178892.1

Genome annotation databases

EnsemblFungiiYER001W; YER001W; YER001W
GeneIDi856718
KEGGisce:YER001W

Similar proteinsi

Entry informationi

Entry nameiMNN1_YEAST
AccessioniPrimary (citable) accession number: P39106
Secondary accession number(s): D3DLP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health