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P39105

- PLB1_YEAST

UniProt

P39105 - PLB1_YEAST

Protein

Lysophospholipase 1

Gene

PLB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol >> phosphatidylcholine > phosphatidylethanolamine.2 Publications

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    GO - Molecular functioni

    1. lysophospholipase activity Source: SGD

    GO - Biological processi

    1. glycerophospholipid metabolic process Source: SGD
    2. phosphatidylcholine acyl-chain remodeling Source: SGD
    3. phospholipid catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:YMR008C-MONOMER.
    YEAST:YMR008C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysophospholipase 1 (EC:3.1.1.5)
    Alternative name(s):
    Phospholipase B 1
    Gene namesi
    Name:PLB1
    Ordered Locus Names:YMR008C
    ORF Names:YM8270.10C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR008c.
    SGDiS000004610. PLB1.

    Subcellular locationi

    Cell membrane 2 Publications; Lipid-anchorGPI-anchor 2 Publications

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. periplasmic space Source: SGD
    3. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 634612Lysophospholipase 1PRO_0000024646Add
    BLAST
    Propeptidei635 – 66430Removed in mature formSequence AnalysisPRO_0000372442Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi52 – 521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi459 – 4591N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi541 – 5411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi565 – 5651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi582 – 5821N-linked (GlcNAc...)Sequence Analysis
    Lipidationi634 – 6341GPI-anchor amidated asparagineSequence Analysis

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    MaxQBiP39105.
    PaxDbiP39105.
    PeptideAtlasiP39105.

    Expressioni

    Gene expression databases

    GenevestigatoriP39105.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    YHR080CP388001EBI-13462,EBI-24597

    Protein-protein interaction databases

    BioGridi35177. 22 interactions.
    DIPiDIP-2804N.
    IntActiP39105. 2 interactions.
    MINTiMINT-1717589.
    STRINGi4932.YMR008C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39105.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 586552PLA2cPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi587 – 62943Ala/Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the lysophospholipase family.Curated
    Contains 1 PLA2c domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG271081.
    GeneTreeiENSGT00510000051940.
    HOGENOMiHOG000189547.
    KOiK13333.
    OMAiNIAEANF.
    OrthoDBiEOG7N37NC.

    Family and domain databases

    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view]
    PfamiPF01735. PLA2_B. 1 hit.
    [Graphical view]
    SMARTiSM00022. PLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 1 hit.
    PROSITEiPS51210. PLA2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39105-1 [UniParc]FASTAAdd to Basket

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    MKLQSLLVSA AVLTSLTENV NAWSPNNSYV PANVTCDDDI NLVREASGLS    50
    DNETEWLKKR DAYTKEALHS FLNRATSNFS DTSLLSTLFG SNSSNMPKIA 100
    VACSGGGYRA MLSGAGMLAA MDNRTDGANE HGLGGLLQGA TYLAGLSGGN 150
    WLTSTLAWNN WTSVQAIVDN TTESNSIWDI SHSILTPDGI NIFKTGSRWD 200
    DISDDVQDKK DAGFNISLAD VWGRALAYNF WPSLHRGGVG YTWSTLREAD 250
    VFKNGEMPFP ITVADGRYPG TTVINLNATL FEFNPFEMGS WDPTLNAFTD 300
    VKYLGTNVTN GKPVNKGQCI AGFDNTGFIT ATSSTLFNQF LLRLNSTDLP 350
    SFIANLATDF LEDLSDNSDD IAIYAPNPFK EANFLQKNAT SSIIESEYLF 400
    LVDGGEDNQN IPLVPLLQKE RELDVIFALD NSADTDDYWP DGASLVNTYQ 450
    RQFGSQGLNL SFPYVPDVNT FVNLGLNKKP TFFGCDARNL TDLEYIPPLI 500
    VYIPNSRHSF NGNQSTFKMS YSDSERLGMI KNGFEAATMG NFTDDSDFLG 550
    CVGCAIIRRK QQNLNATLPS ECSQCFTNYC WNGTIDSRSV SGVGNDDYSS 600
    SASLSASAAA ASASASASAS ASASASGSST HKKNAGNALV NYSNLNTNTF 650
    IGVLSVISAV FGLI 664
    Length:664
    Mass (Da):71,667
    Last modified:February 1, 1996 - v2
    Checksum:iE05A585E7AB73F34
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti32 – 321A → S in AAA61611. (PubMed:8051052)Curated
    Sequence conflicti93 – 931S → P in AAT92807. (PubMed:17322287)Curated
    Sequence conflicti494 – 4941E → D in AAA61611. (PubMed:8051052)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23089 Genomic DNA. Translation: AAA61611.1.
    Z48613 Genomic DNA. Translation: CAA88523.1.
    AY692788 Genomic DNA. Translation: AAT92807.1.
    BK006946 Genomic DNA. Translation: DAA09906.1.
    PIRiS53037.
    RefSeqiNP_013721.1. NM_001182504.1.

    Genome annotation databases

    EnsemblFungiiYMR008C; YMR008C; YMR008C.
    GeneIDi855020.
    KEGGisce:YMR008C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23089 Genomic DNA. Translation: AAA61611.1 .
    Z48613 Genomic DNA. Translation: CAA88523.1 .
    AY692788 Genomic DNA. Translation: AAT92807.1 .
    BK006946 Genomic DNA. Translation: DAA09906.1 .
    PIRi S53037.
    RefSeqi NP_013721.1. NM_001182504.1.

    3D structure databases

    ProteinModelPortali P39105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35177. 22 interactions.
    DIPi DIP-2804N.
    IntActi P39105. 2 interactions.
    MINTi MINT-1717589.
    STRINGi 4932.YMR008C.

    Proteomic databases

    MaxQBi P39105.
    PaxDbi P39105.
    PeptideAtlasi P39105.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR008C ; YMR008C ; YMR008C .
    GeneIDi 855020.
    KEGGi sce:YMR008C.

    Organism-specific databases

    CYGDi YMR008c.
    SGDi S000004610. PLB1.

    Phylogenomic databases

    eggNOGi NOG271081.
    GeneTreei ENSGT00510000051940.
    HOGENOMi HOG000189547.
    KOi K13333.
    OMAi NIAEANF.
    OrthoDBi EOG7N37NC.

    Enzyme and pathway databases

    BioCyci MetaCyc:YMR008C-MONOMER.
    YEAST:YMR008C-MONOMER.

    Miscellaneous databases

    NextBioi 978204.

    Gene expression databases

    Genevestigatori P39105.

    Family and domain databases

    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view ]
    Pfami PF01735. PLA2_B. 1 hit.
    [Graphical view ]
    SMARTi SM00022. PLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52151. SSF52151. 1 hit.
    PROSITEi PS51210. PLA2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Saccharomyces cerevisiae PLB1 gene encodes a protein required for lysophospholipase and phospholipase B activity."
      Lee K.S., Patton J.L., Fido M., Hines L.K., Kohlwein S.D., Paltauf F., Henry S.A., Levin D.E.
      J. Biol. Chem. 269:19725-19730(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 204508 / S288c.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins."
      Hamada K., Terashima H., Arisawa M., Yabuki N., Kitada K.
      J. Bacteriol. 181:3886-3889(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "Characterization and function in vivo of two novel phospholipases B/lysophospholipases from Saccharomyces cerevisiae."
      Merkel O., Fido M., Mayr J.A., Prueger H., Raab F., Zandonella G., Kohlwein S.D., Paltauf F.
      J. Biol. Chem. 274:28121-28127(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPLB1_YEAST
    AccessioniPrimary (citable) accession number: P39105
    Secondary accession number(s): D6VZI2, Q6B2E2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3