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P39105

- PLB1_YEAST

UniProt

P39105 - PLB1_YEAST

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Protein
Lysophospholipase 1
Gene
PLB1, YMR008C, YM8270.10C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol >> phosphatidylcholine > phosphatidylethanolamine.2 Publications

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

GO - Molecular functioni

  1. lysophospholipase activity Source: SGD

GO - Biological processi

  1. glycerophospholipid metabolic process Source: SGD
  2. phosphatidylcholine acyl-chain remodeling Source: SGD
  3. phospholipid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YMR008C-MONOMER.
YEAST:YMR008C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase 1 (EC:3.1.1.5)
Alternative name(s):
Phospholipase B 1
Gene namesi
Name:PLB1
Ordered Locus Names:YMR008C
ORF Names:YM8270.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR008c.
SGDiS000004610. PLB1.

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor Inferred 2 Publications

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. periplasmic space Source: SGD
  3. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 634612Lysophospholipase 1
PRO_0000024646Add
BLAST
Propeptidei635 – 66430Removed in mature form Reviewed prediction
PRO_0000372442Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi26 – 261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi33 – 331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi52 – 521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi78 – 781N-linked (GlcNAc...) Reviewed prediction
Glycosylationi92 – 921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi123 – 1231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi160 – 1601N-linked (GlcNAc...) Reviewed prediction
Glycosylationi170 – 1701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi215 – 2151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi277 – 2771N-linked (GlcNAc...) Reviewed prediction
Glycosylationi307 – 3071N-linked (GlcNAc...) Reviewed prediction
Glycosylationi345 – 3451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi388 – 3881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi459 – 4591N-linked (GlcNAc...) Reviewed prediction
Glycosylationi489 – 4891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi513 – 5131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi541 – 5411N-linked (GlcNAc...) Reviewed prediction
Glycosylationi565 – 5651N-linked (GlcNAc...) Reviewed prediction
Glycosylationi582 – 5821N-linked (GlcNAc...) Reviewed prediction
Lipidationi634 – 6341GPI-anchor amidated asparagine Reviewed prediction

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP39105.
PaxDbiP39105.
PeptideAtlasiP39105.

Expressioni

Gene expression databases

GenevestigatoriP39105.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
YHR080CP388001EBI-13462,EBI-24597

Protein-protein interaction databases

BioGridi35177. 22 interactions.
DIPiDIP-2804N.
IntActiP39105. 2 interactions.
MINTiMINT-1717589.
STRINGi4932.YMR008C.

Structurei

3D structure databases

ProteinModelPortaliP39105.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 586552PLA2c
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi587 – 62943Ala/Ser-rich
Add
BLAST

Sequence similaritiesi

Belongs to the lysophospholipase family.
Contains 1 PLA2c domain.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG271081.
GeneTreeiENSGT00510000051940.
HOGENOMiHOG000189547.
KOiK13333.
OMAiNIAEANF.
OrthoDBiEOG7N37NC.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39105-1 [UniParc]FASTAAdd to Basket

« Hide

MKLQSLLVSA AVLTSLTENV NAWSPNNSYV PANVTCDDDI NLVREASGLS    50
DNETEWLKKR DAYTKEALHS FLNRATSNFS DTSLLSTLFG SNSSNMPKIA 100
VACSGGGYRA MLSGAGMLAA MDNRTDGANE HGLGGLLQGA TYLAGLSGGN 150
WLTSTLAWNN WTSVQAIVDN TTESNSIWDI SHSILTPDGI NIFKTGSRWD 200
DISDDVQDKK DAGFNISLAD VWGRALAYNF WPSLHRGGVG YTWSTLREAD 250
VFKNGEMPFP ITVADGRYPG TTVINLNATL FEFNPFEMGS WDPTLNAFTD 300
VKYLGTNVTN GKPVNKGQCI AGFDNTGFIT ATSSTLFNQF LLRLNSTDLP 350
SFIANLATDF LEDLSDNSDD IAIYAPNPFK EANFLQKNAT SSIIESEYLF 400
LVDGGEDNQN IPLVPLLQKE RELDVIFALD NSADTDDYWP DGASLVNTYQ 450
RQFGSQGLNL SFPYVPDVNT FVNLGLNKKP TFFGCDARNL TDLEYIPPLI 500
VYIPNSRHSF NGNQSTFKMS YSDSERLGMI KNGFEAATMG NFTDDSDFLG 550
CVGCAIIRRK QQNLNATLPS ECSQCFTNYC WNGTIDSRSV SGVGNDDYSS 600
SASLSASAAA ASASASASAS ASASASGSST HKKNAGNALV NYSNLNTNTF 650
IGVLSVISAV FGLI 664
Length:664
Mass (Da):71,667
Last modified:February 1, 1996 - v2
Checksum:iE05A585E7AB73F34
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321A → S in AAA61611. 1 Publication
Sequence conflicti93 – 931S → P in AAT92807. 1 Publication
Sequence conflicti494 – 4941E → D in AAA61611. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23089 Genomic DNA. Translation: AAA61611.1.
Z48613 Genomic DNA. Translation: CAA88523.1.
AY692788 Genomic DNA. Translation: AAT92807.1.
BK006946 Genomic DNA. Translation: DAA09906.1.
PIRiS53037.
RefSeqiNP_013721.1. NM_001182504.1.

Genome annotation databases

EnsemblFungiiYMR008C; YMR008C; YMR008C.
GeneIDi855020.
KEGGisce:YMR008C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23089 Genomic DNA. Translation: AAA61611.1 .
Z48613 Genomic DNA. Translation: CAA88523.1 .
AY692788 Genomic DNA. Translation: AAT92807.1 .
BK006946 Genomic DNA. Translation: DAA09906.1 .
PIRi S53037.
RefSeqi NP_013721.1. NM_001182504.1.

3D structure databases

ProteinModelPortali P39105.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35177. 22 interactions.
DIPi DIP-2804N.
IntActi P39105. 2 interactions.
MINTi MINT-1717589.
STRINGi 4932.YMR008C.

Proteomic databases

MaxQBi P39105.
PaxDbi P39105.
PeptideAtlasi P39105.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR008C ; YMR008C ; YMR008C .
GeneIDi 855020.
KEGGi sce:YMR008C.

Organism-specific databases

CYGDi YMR008c.
SGDi S000004610. PLB1.

Phylogenomic databases

eggNOGi NOG271081.
GeneTreei ENSGT00510000051940.
HOGENOMi HOG000189547.
KOi K13333.
OMAi NIAEANF.
OrthoDBi EOG7N37NC.

Enzyme and pathway databases

BioCyci MetaCyc:YMR008C-MONOMER.
YEAST:YMR008C-MONOMER.

Miscellaneous databases

NextBioi 978204.

Gene expression databases

Genevestigatori P39105.

Family and domain databases

InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view ]
Pfami PF01735. PLA2_B. 1 hit.
[Graphical view ]
SMARTi SM00022. PLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF52151. SSF52151. 1 hit.
PROSITEi PS51210. PLA2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Saccharomyces cerevisiae PLB1 gene encodes a protein required for lysophospholipase and phospholipase B activity."
    Lee K.S., Patton J.L., Fido M., Hines L.K., Kohlwein S.D., Paltauf F., Henry S.A., Levin D.E.
    J. Biol. Chem. 269:19725-19730(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins."
    Hamada K., Terashima H., Arisawa M., Yabuki N., Kitada K.
    J. Bacteriol. 181:3886-3889(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Characterization and function in vivo of two novel phospholipases B/lysophospholipases from Saccharomyces cerevisiae."
    Merkel O., Fido M., Mayr J.A., Prueger H., Raab F., Zandonella G., Kohlwein S.D., Paltauf F.
    J. Biol. Chem. 274:28121-28127(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPLB1_YEAST
AccessioniPrimary (citable) accession number: P39105
Secondary accession number(s): D6VZI2, Q6B2E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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