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P39105 (PLB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysophospholipase 1

EC=3.1.1.5
Alternative name(s):
Phospholipase B 1
Gene names
Name:PLB1
Ordered Locus Names:YMR008C
ORF Names:YM8270.10C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length664 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol >> phosphatidylcholine > phosphatidylethanolamine. Ref.1 Ref.6

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Probable Ref.5 Ref.6.

Sequence similarities

Belongs to the lysophospholipase family.

Contains 1 PLA2c domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

YHR080CP388001EBI-13462,EBI-24597

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 634612Lysophospholipase 1
PRO_0000024646
Propeptide635 – 66430Removed in mature form Potential
PRO_0000372442

Regions

Domain35 – 586552PLA2c
Compositional bias587 – 62943Ala/Ser-rich

Amino acid modifications

Lipidation6341GPI-anchor amidated asparagine Potential
Glycosylation261N-linked (GlcNAc...) Potential
Glycosylation331N-linked (GlcNAc...) Potential
Glycosylation521N-linked (GlcNAc...) Potential
Glycosylation781N-linked (GlcNAc...) Potential
Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation1231N-linked (GlcNAc...) Potential
Glycosylation1601N-linked (GlcNAc...) Potential
Glycosylation1701N-linked (GlcNAc...) Potential
Glycosylation2151N-linked (GlcNAc...) Potential
Glycosylation2771N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation3451N-linked (GlcNAc...) Potential
Glycosylation3881N-linked (GlcNAc...) Potential
Glycosylation4591N-linked (GlcNAc...) Potential
Glycosylation4891N-linked (GlcNAc...) Potential
Glycosylation5131N-linked (GlcNAc...) Potential
Glycosylation5411N-linked (GlcNAc...) Potential
Glycosylation5651N-linked (GlcNAc...) Potential
Glycosylation5821N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict321A → S in AAA61611. Ref.1
Sequence conflict931S → P in AAT92807. Ref.4
Sequence conflict4941E → D in AAA61611. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39105 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: E05A585E7AB73F34

FASTA66471,667
        10         20         30         40         50         60 
MKLQSLLVSA AVLTSLTENV NAWSPNNSYV PANVTCDDDI NLVREASGLS DNETEWLKKR 

        70         80         90        100        110        120 
DAYTKEALHS FLNRATSNFS DTSLLSTLFG SNSSNMPKIA VACSGGGYRA MLSGAGMLAA 

       130        140        150        160        170        180 
MDNRTDGANE HGLGGLLQGA TYLAGLSGGN WLTSTLAWNN WTSVQAIVDN TTESNSIWDI 

       190        200        210        220        230        240 
SHSILTPDGI NIFKTGSRWD DISDDVQDKK DAGFNISLAD VWGRALAYNF WPSLHRGGVG 

       250        260        270        280        290        300 
YTWSTLREAD VFKNGEMPFP ITVADGRYPG TTVINLNATL FEFNPFEMGS WDPTLNAFTD 

       310        320        330        340        350        360 
VKYLGTNVTN GKPVNKGQCI AGFDNTGFIT ATSSTLFNQF LLRLNSTDLP SFIANLATDF 

       370        380        390        400        410        420 
LEDLSDNSDD IAIYAPNPFK EANFLQKNAT SSIIESEYLF LVDGGEDNQN IPLVPLLQKE 

       430        440        450        460        470        480 
RELDVIFALD NSADTDDYWP DGASLVNTYQ RQFGSQGLNL SFPYVPDVNT FVNLGLNKKP 

       490        500        510        520        530        540 
TFFGCDARNL TDLEYIPPLI VYIPNSRHSF NGNQSTFKMS YSDSERLGMI KNGFEAATMG 

       550        560        570        580        590        600 
NFTDDSDFLG CVGCAIIRRK QQNLNATLPS ECSQCFTNYC WNGTIDSRSV SGVGNDDYSS 

       610        620        630        640        650        660 
SASLSASAAA ASASASASAS ASASASGSST HKKNAGNALV NYSNLNTNTF IGVLSVISAV 


FGLI 

« Hide

References

« Hide 'large scale' references
[1]"The Saccharomyces cerevisiae PLB1 gene encodes a protein required for lysophospholipase and phospholipase B activity."
Lee K.S., Patton J.L., Fido M., Hines L.K., Kohlwein S.D., Paltauf F., Henry S.A., Levin D.E.
J. Biol. Chem. 269:19725-19730(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins."
Hamada K., Terashima H., Arisawa M., Yabuki N., Kitada K.
J. Bacteriol. 181:3886-3889(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Characterization and function in vivo of two novel phospholipases B/lysophospholipases from Saccharomyces cerevisiae."
Merkel O., Fido M., Mayr J.A., Prueger H., Raab F., Zandonella G., Kohlwein S.D., Paltauf F.
J. Biol. Chem. 274:28121-28127(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23089 Genomic DNA. Translation: AAA61611.1.
Z48613 Genomic DNA. Translation: CAA88523.1.
AY692788 Genomic DNA. Translation: AAT92807.1.
BK006946 Genomic DNA. Translation: DAA09906.1.
PIRS53037.
RefSeqNP_013721.1. NM_001182504.1.

3D structure databases

ProteinModelPortalP39105.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35177. 22 interactions.
DIPDIP-2804N.
IntActP39105. 2 interactions.
MINTMINT-1717589.
STRING4932.YMR008C.

Proteomic databases

MaxQBP39105.
PaxDbP39105.
PeptideAtlasP39105.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR008C; YMR008C; YMR008C.
GeneID855020.
KEGGsce:YMR008C.

Organism-specific databases

CYGDYMR008c.
SGDS000004610. PLB1.

Phylogenomic databases

eggNOGNOG271081.
GeneTreeENSGT00510000051940.
HOGENOMHOG000189547.
KOK13333.
OMANIAEANF.
OrthoDBEOG7N37NC.

Enzyme and pathway databases

BioCycMetaCyc:YMR008C-MONOMER.
YEAST:YMR008C-MONOMER.

Gene expression databases

GenevestigatorP39105.

Family and domain databases

InterProIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMSSF52151. SSF52151. 1 hit.
PROSITEPS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978204.

Entry information

Entry namePLB1_YEAST
AccessionPrimary (citable) accession number: P39105
Secondary accession number(s): D6VZI2, Q6B2E2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families