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Protein

Phosphatidylinositol 4-kinase PIK1

Gene

PIK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol 1,4,5,-trisphosphate. PIK1 is part of a nuclear phosphoinositide cycle and could control cytokinesis through the actin cytoskeleton.

Miscellaneous

Present with 1600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.

GO - Molecular functioni

  • 1-phosphatidylinositol 4-kinase activity Source: SGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • endocytosis Source: CACAO
  • phosphatidylinositol-mediated signaling Source: GO_Central
  • phosphatidylinositol phosphorylation Source: SGD
  • positive regulation of Golgi to plasma membrane protein transport Source: CACAO
  • positive regulation of protein secretion Source: CACAO

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YNL267W-MONOMER
ReactomeiR-SCE-1660514 Synthesis of PIPs at the Golgi membrane

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase PIK1 (EC:2.7.1.67)
Short name:
PI4-kinase
Short name:
PtdIns-4-kinase
Gene namesi
Name:PIK1
Ordered Locus Names:YNL267W
ORF Names:N0795
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL267W
SGDiS000005211 PIK1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000888341 – 1066Phosphatidylinositol 4-kinase PIK1Add BLAST1066

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1
Modified residuei236PhosphoserineCombined sources1
Modified residuei384PhosphoserineCombined sources1
Modified residuei394PhosphothreonineCombined sources1
Modified residuei396PhosphoserineCombined sources1
Modified residuei592PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39104
PaxDbiP39104
PRIDEiP39104

PTM databases

iPTMnetiP39104

Interactioni

Subunit structurei

Interacts with FRQ1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35573, 246 interactors
DIPiDIP-6770N
IntActiP39104, 8 interactors
MINTiP39104
STRINGi4932.YNL267W

Structurei

Secondary structure

11066
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi126 – 135Combined sources10
Helixi156 – 168Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JU0NMR-B121-172[»]
ProteinModelPortaliP39104
SMRiP39104
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39104

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 133PIK helicalPROSITE-ProRule annotationAdd BLAST133
Domaini793 – 1041PI3K/PI4KPROSITE-ProRule annotationAdd BLAST249

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074892
HOGENOMiHOG000248748
InParanoidiP39104
KOiK19801
OMAiVHSIKKS
OrthoDBiEOG092C0K2T

Family and domain databases

Gene3Di1.10.1070.11, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR011009 Kinase-like_dom_sf
IPR021601 Phosphatidylino_kinase_fungi
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR015433 PI_Kinase
IPR001263 PInositide-3_kin_accessory_dom
PANTHERiPTHR10048 PTHR10048, 1 hit
PfamiView protein in Pfam
PF00454 PI3_PI4_kinase, 1 hit
PF00613 PI3Ka, 1 hit
PF11522 Pik1, 1 hit
SMARTiView protein in SMART
SM00146 PI3Kc, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS51545 PIK_HELICAL, 1 hit

Sequencei

Sequence statusi: Complete.

P39104-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKASSSKKS FDDTIELKKN EQLLKLINSS EFTLHNCVEL LCKHSENIGI
60 70 80 90 100
HYYLCQKLAT FPHSELQFYI PQLVQVLVTM ETESMALEDL LLRLRAENPH
110 120 130 140 150
FALLTFWQLQ ALLTDLSTDP ASYGFQVARR VLNNLQTNLF NTSSGSDKNV
160 170 180 190 200
KIHENVAPAL VLSSMIMSAI AFPQLSEVTK PLVESQGRRQ KAFVFKLARS
210 220 230 240 250
AMKDFTKNMT LKNTLLNKKT SRSKRVSSNR SSTPTSPIDL IDPIKTKEDA
260 270 280 290 300
SFRKSRHSEV KLDFDIVDDI GNQVFEERIS SSIKLPKRKP KYLDNSYVHR
310 320 330 340 350
TYDGKNINRD GSISNTAKAL DGNKGDYISP KGRNDENNEI GNNEDETGGE
360 370 380 390 400
TEEDADALNS DHFTSSMPDL HNIQPRTSSA SSASLEGTPK LNRTNSQPLS
410 420 430 440 450
RQAFKNSKKA NSSLSQEIDL SQLSTTSKIK MLKANYFRCE TQFAIALETI
460 470 480 490 500
SQRLARVPTE ARLSALRAEL FLLNRDLPAE VDIPTLLPPN KKGKLHKLVT
510 520 530 540 550
ITANEAQVLN SAEKVPYLLL IEYLRDEFDF DPTSETNERL LKKISGNQGG
560 570 580 590 600
LIFDLNYMNR KENNENRNES TLTSNNTRSS VYDSNSFNNG ASRNEGLSST
610 620 630 640 650
SRSDSASTAH VRTEVNKEED LGDMSMVKVR NRTDDEAYRN ALVIQSAANV
660 670 680 690 700
PILPDDSQDR SPELNFGSNL DEVLIENGIN SKNIHSQTDA LADQMRVSAV
710 720 730 740 750
MLAQLDKSPQ QLSESTKQIR AQIISSMKEV QDKFGYHDLE ALHGMAGERK
760 770 780 790 800
LENDLMTGGI DTSYLGEDWA TKKERIRKTS EYGHFENWDL CSVIAKTGDD
810 820 830 840 850
LRQEAFAYQM IQAMANIWVK EKVDVWVKRM KILITSANTG LVETITNAMS
860 870 880 890 900
VHSIKKALTK KMIEDAELDD KGGIASLNDH FLRAFGNPNG FKYRRAQDNF
910 920 930 940 950
ASSLAAYSVI CYLLQVKDRH NGNIMIDNEG HVSHIDFGFM LSNSPGSVGF
960 970 980 990 1000
EAAPFKLTYE YIELLGGVEG EAFKKFVELT KSSFKALRKY ADQIVSMCEI
1010 1020 1030 1040 1050
MQKDNMQPCF DAGEQTSVQL RQRFQLDLSE KEVDDFVENF LIGKSLGSIY
1060
TRIYDQFQLI TQGIYS
Length:1,066
Mass (Da):119,923
Last modified:February 1, 1995 - v1
Checksum:i7666979CA14B1CB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76058 Genomic DNA Translation: CAA53658.1
L20220 Genomic DNA Translation: AAA34873.1
X92494 Genomic DNA Translation: CAA63231.1
Z71543 Genomic DNA Translation: CAA96174.1
BK006947 Genomic DNA Translation: DAA10293.1
PIRiA49335
RefSeqiNP_014132.1, NM_001183105.1

Genome annotation databases

EnsemblFungiiYNL267W; YNL267W; YNL267W
GeneIDi855454
KEGGisce:YNL267W

Similar proteinsi

Entry informationi

Entry nameiPIK1_YEAST
AccessioniPrimary (citable) accession number: P39104
Secondary accession number(s): D6W0S7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health