Reviewed,
UniProtKB/Swiss-Prot P39098 (MA1A2_MOUSE)
Last modified
November 3, 2009.
Version 91.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB EC=3.2.1.113 Alternative name(s): Processing alpha-1,2-mannosidase IB Short name=Alpha-1,2-mannosidase IB Mannosidase alpha class 1A member 2 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 641 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2. |
| Catalytic activity | Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2. |
| Cofactor | Calcium. |
| Enzyme regulation | Inhibited by both 1-deoxymannojirimycin and kifunensine By similarity. |
| Pathway | |
| Subcellular location | Golgi apparatus membrane; Single-pass type II membrane protein. |
| Sequence similarities | Belongs to the glycosyl hydrolase 47 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Golgi apparatus Membrane |
| Coding sequence diversity | Alternative splicing |
| Domain | Signal-anchor Transmembrane |
| Ligand | Calcium |
| Molecular function | Glycosidase Hydrolase |
| PTM | Glycoprotein |
| Gene Ontology (GO) | |
| Biological process | glycoprotein metabolic process Inferred from mutant phenotype. Source: MGI lung alveolus developmentInferred from mutant phenotype. Source: MGI respiratory gaseous exchangeInferred from mutant phenotype. Source: MGI |
| Cellular component | Golgi membrane Inferred from direct assay. Source: MGI integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW mannosyl-oligosaccharide 1,2-alpha-mannosidase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P39098-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P39098-2) The sequence of this isoform differs from the canonical sequence as follows: 259-285: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 641 | 641 | Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB | PRO_0000210313 | |||||
Regions | |||||||||
| Topological domain | 1 – 36 | 36 | Cytoplasmic Potential | ||||||
| Transmembrane | 37 – 57 | 21 | Signal-anchor for type II membrane protein Potential | ||||||
| Topological domain | 58 – 641 | 584 | Lumenal Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 631 | 1 | N-linked (GlcNAc...) Potential | ||||||
Natural variations | |||||||||
| Alternative sequence | 259 – 285 | 27 | Missing in isoform 2. | VSP_018615 | |||||
Experimental info | |||||||||
| Sequence conflict | 411 | 1 | M → T in AAB60438. Ref.1 | ||||||
| Sequence conflict | 468 | 1 | F → L in AAB60438. Ref.1 | ||||||
| Sequence conflict | 592 | 1 | F → S in AAB60438. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation of a mouse Golgi mannosidase cDNA, a member of a gene family conserved from yeast to mammals." Herscovics A.A., Schneikert J., Athanassiadis A., Moremen K.W. J. Biol. Chem. 269:9864-9871(1994) [PubMed: 8144579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: BALB/c. Tissue: Fibroblast. |
| [2] | "Genomic organization and chromosomal mapping of the murine alpha 1,2-mannosidase IB involved in N-glycan maturation." Campbell-Dyke N., Athanassiadis A., Herscovics A. Genomics 41:155-159(1997) [PubMed: 9143489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Ola. |
| [3] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Bone marrow macrophage and Hippocampus. |
| [4] | "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NOD. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U03458 mRNA. Translation: AAB60439.1. U03457 mRNA. Translation: AAB60438.1. AF078095 AF078094 Genomic DNA. Translation: AAC34829.1. AK013515 mRNA. Translation: BAB28892.3. AK153336 mRNA. Translation: BAE31915.1. AL645760 Genomic DNA. Translation: CAI26213.1. AL645760 Genomic DNA. Translation: CAI26214.1. BC049121 mRNA. Translation: AAH49121.1. BC068192 mRNA. Translation: AAH68192.1. | |
| IPI | IPI00131609. IPI00515213. |
| PIR | A54407. |
| RefSeq | NP_034893.1. |
| UniGene | Mm.271617 Mm.297043 Mm.458282 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1DL2 based on UniProtKB P32906. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P39098. |
Protein family/group databases | |
| CAZy | GH47. Glycoside Hydrolase Family 47. |
Proteomic databases | |
| PRIDE | P39098. |
Genome annotation databases | |
| Ensembl | ENSMUST00000008907; ENSMUSP00000008907; ENSMUSG00000008763; Mus musculus. [Genome view] ENSMUST00000090730; ENSMUSP00000088232; ENSMUSG00000008763; Mus musculus. [Genome view] |
| GeneID | 17156. |
| KEGG | mmu:17156. |
| UCSC | uc008qqw.1. mouse. |
Organism-specific databases | |
| CTD | 17156. |
| MGI | MGI:104676. Man1a2. |
Phylogenomic databases | |
| HOGENOM | P39098. |
| HOVERGEN | P39098. |
| OMA | DHEARKM. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.113. 244. |
Gene expression databases | |
| ArrayExpress | P39098. |
| Bgee | P39098. |
| CleanEx | MM_MAN1A2. |
| Genevestigator | P39098. |
| GermOnline | ENSMUSG00000008763. Mus musculus. |
Family and domain databases | |
| InterPro | IPR001382. Glyco_hydro_47. [Graphical view] |
| Gene3D | G3DSA:1.50.10.50. Glyco_hydro_47. 1 hit. |
| PANTHER | PTHR11742. Glyco_hydro_47. 1 hit. |
| Pfam | PF01532. Glyco_hydro_47. 1 hit. [Graphical view] |
| PRINTS | PR00747. GLYHDRLASE47. |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 291422. |
| SOURCE | Search... |
Entry information
| Entry name | MA1A2_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P39098 Secondary accession number(s): Q5SUY6 Q9CUY9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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