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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB

Gene

Man1a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity

Enzyme regulationi

Inhibited by both 1-deoxymannojirimycin and kifunensine.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei508Proton donorBy similarity1
Metal bindingi619CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glycoprotein metabolic process Source: MGI
  • lung alveolus development Source: MGI
  • N-glycan processing Source: GO_Central
  • protein glycosylation Source: UniProtKB-UniPathway
  • respiratory gaseous exchange Source: MGI

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.2.1.113 3474
ReactomeiR-MMU-6811438 Intra-Golgi traffic
R-MMU-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2
UniPathwayiUPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC:3.2.1.113By similarity)
Alternative name(s):
Mannosidase alpha class 1A member 2
Processing alpha-1,2-mannosidase IB
Short name:
Alpha-1,2-mannosidase IB
Gene namesi
Name:Man1a2
Synonyms:Man1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:104676 Man1a2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 36CytoplasmicSequence analysisAdd BLAST35
Transmembranei37 – 57Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini58 – 641LumenalSequence analysisAdd BLAST584

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002103132 – 641Mannosyl-oligosaccharide 1,2-alpha-mannosidase IBAdd BLAST640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Disulfide bondi462 ↔ 494By similarity
Glycosylationi631N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP39098
PaxDbiP39098
PeptideAtlasiP39098
PRIDEiP39098

PTM databases

iPTMnetiP39098
PhosphoSitePlusiP39098

Expressioni

Gene expression databases

BgeeiENSMUSG00000008763
CleanExiMM_MAN1A2
ExpressionAtlasiP39098 baseline and differential
GenevisibleiP39098 MM

Interactioni

Protein-protein interaction databases

BioGridi201303, 1 interactor
IntActiP39098, 1 interactor
MINTiP39098
STRINGi10090.ENSMUSP00000008907

Structurei

3D structure databases

ProteinModelPortaliP39098
SMRiP39098
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
GeneTreeiENSGT00390000016529
HOGENOMiHOG000181988
HOVERGENiHBG052389
InParanoidiP39098
KOiK01230
OMAiQYGWGHN
OrthoDBiEOG091G04C8
PhylomeDBiP39098
TreeFamiTF313420

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P39098-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC
60 70 80 90 100
FGAFFFLPDS SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH
110 120 130 140 150
REEEERLRNK IRADHEKALE EAKEKLRKSR EEIRAEIQTE KNKVAQAMKT
160 170 180 190 200
KETRVLPPVP VPQRVGVSGG DPEDMEIKKK RDKIKEMMKH AWDNYRTYGW
210 220 230 240 250
GHNELRPIAR KGHSTNIFGS SQMGATIVDA LDTLYIMGLH DEFMDGQRWI
260 270 280 290 300
EENLDFSVNS EVSVFEVNIR FIGGLLAAYY LSGEEIFKTK AVQLAEKLLP
310 320 330 340 350
AFNTPTGIPW AMVNLKSGVG RNWGWASAGS SILAEFGTLH MEFVHLSYLT
360 370 380 390 400
GDLTYYNKVM HIRKLLQKME RPNGLYPNYL NPRTGRWGQY HTSVGGLGDS
410 420 430 440 450
FYEYLLKAWL MSDKTDHEAR RMYDDAVEAI EKHLIKKSRG GLVFIGEWKN
460 470 480 490 500
GHLERKMGHL ACFAGGMFAL GADGSRKDKA GHYLELGAEI ARTCHESYDR
510 520 530 540 550
TALKLGPESF KFDGAVEAVA VRQAEKYYIL RPEVIETYWY LWRFTHDPRY
560 570 580 590 600
RQWGWEAALA IEKSCRVSGG FSGVKDVYAP TPVHDDVQQS FFLAETLKYL
610 620 630 640
YLLFSGDDLL PLDHWVFNTE AHPLPVLRLA NSTLSGNPAV R
Length:641
Mass (Da):72,871
Last modified:February 1, 1995 - v1
Checksum:i7747FE57CCB2C4C5
GO
Isoform 2 (identifier: P39098-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-285: Missing.

Note: No experimental confirmation available.
Show »
Length:614
Mass (Da):69,912
Checksum:i43E504696E83116B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti411M → T in AAB60438 (PubMed:8144579).Curated1
Sequence conflicti468F → L in AAB60438 (PubMed:8144579).Curated1
Sequence conflicti592F → S in AAB60438 (PubMed:8144579).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018615259 – 285Missing in isoform 2. CuratedAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03458 mRNA Translation: AAB60439.1
U03457 mRNA Translation: AAB60438.1
AF078095
, AF078083, AF078084, AF078085, AF078086, AF078087, AF078088, AF078089, AF078090, AF078091, AF078092, AF078093, AF078094 Genomic DNA Translation: AAC34829.1
AK013515 mRNA Translation: BAB28892.3
AK153336 mRNA Translation: BAE31915.1
AL645760 Genomic DNA Translation: CAI26213.1
AL645760 Genomic DNA Translation: CAI26214.1
BC049121 mRNA Translation: AAH49121.1
BC068192 mRNA Translation: AAH68192.1
CCDSiCCDS17676.1 [P39098-1]
PIRiA54407
RefSeqiNP_034893.1, NM_010763.2 [P39098-1]
UniGeneiMm.271617

Genome annotation databases

EnsembliENSMUST00000008907; ENSMUSP00000008907; ENSMUSG00000008763 [P39098-1]
GeneIDi17156
KEGGimmu:17156
UCSCiuc008qqw.3 mouse [P39098-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMA1A2_MOUSE
AccessioniPrimary (citable) accession number: P39098
Secondary accession number(s): Q5SUY6
, Q5SUY7, Q60599, Q9CUY9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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