Reviewed, UniProtKB/Swiss-Prot P39098 (MA1A2_MOUSE)

Names and origin

Protein names

Recommended name:
    Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB
    EC=3.2.1.113
Alternative name(s):
    Processing alpha-1,2-mannosidase IB
      Short name=Alpha-1,2-mannosidase IB
    Mannosidase alpha class 1A member 2

Gene names

Name:	Man1a2
Synonyms:	Man1b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
Catalytic activity	Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.
Cofactor	Calcium.
Enzyme regulation	Inhibited by both 1-deoxymannojirimycin and kifunensine By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyl hydrolase 47 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	Calcium
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	glycoprotein metabolic process Inferred from mutant phenotype. Source: MGI lung alveolus development Inferred from mutant phenotype. Source: MGI respiratory gaseous exchange Inferred from mutant phenotype. Source: MGI
Cellular component	Golgi membrane Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P39098-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P39098-2)

The sequence of this isoform differs from the canonical sequence as follows:
     259-285: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB		PRO_0000210313
Regions
	Topological domain	1 – 36	36	Cytoplasmic Potential
	Transmembrane	37 – 57	21	Signal-anchor for type II membrane protein Potential
	Topological domain	58 – 641	584	Lumenal Potential
Amino acid modifications
	Glycosylation	631	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	259 – 285	27	Missing in isoform 2.		VSP_018615
Experimental info
	Sequence conflict	411	1	M → T in AAB60438. Ref.1
	Sequence conflict	468	1	F → L in AAB60438. Ref.1
	Sequence conflict	592	1	F → S in AAB60438. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7747FE57CCB2C4C5	FASTA	641	72,871
	10 20 30 40 50 60 MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC FGAFFFLPDS 70 80 90 100 110 120 SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH REEEERLRNK IRADHEKALE 130 140 150 160 170 180 EAKEKLRKSR EEIRAEIQTE KNKVAQAMKT KETRVLPPVP VPQRVGVSGG DPEDMEIKKK 190 200 210 220 230 240 RDKIKEMMKH AWDNYRTYGW GHNELRPIAR KGHSTNIFGS SQMGATIVDA LDTLYIMGLH 250 260 270 280 290 300 DEFMDGQRWI EENLDFSVNS EVSVFEVNIR FIGGLLAAYY LSGEEIFKTK AVQLAEKLLP 310 320 330 340 350 360 AFNTPTGIPW AMVNLKSGVG RNWGWASAGS SILAEFGTLH MEFVHLSYLT GDLTYYNKVM 370 380 390 400 410 420 HIRKLLQKME RPNGLYPNYL NPRTGRWGQY HTSVGGLGDS FYEYLLKAWL MSDKTDHEAR 430 440 450 460 470 480 RMYDDAVEAI EKHLIKKSRG GLVFIGEWKN GHLERKMGHL ACFAGGMFAL GADGSRKDKA 490 500 510 520 530 540 GHYLELGAEI ARTCHESYDR TALKLGPESF KFDGAVEAVA VRQAEKYYIL RPEVIETYWY 550 560 570 580 590 600 LWRFTHDPRY RQWGWEAALA IEKSCRVSGG FSGVKDVYAP TPVHDDVQQS FFLAETLKYL 610 620 630 640 YLLFSGDDLL PLDHWVFNTE AHPLPVLRLA NSTLSGNPAV R
	Isoform 2. Checksum: 43E504696E83116B	FASTA	614	69,912
	10 20 30 40 50 60 MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC FGAFFFLPDS 70 80 90 100 110 120 SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH REEEERLRNK IRADHEKALE 130 140 150 160 170 180 EAKEKLRKSR EEIRAEIQTE KNKVAQAMKT KETRVLPPVP VPQRVGVSGG DPEDMEIKKK 190 200 210 220 230 240 RDKIKEMMKH AWDNYRTYGW GHNELRPIAR KGHSTNIFGS SQMGATIVDA LDTLYIMGLH 250 260 270 280 290 300 DEFMDGQRWI EENLDFSVIF KTKAVQLAEK LLPAFNTPTG IPWAMVNLKS GVGRNWGWAS 310 320 330 340 350 360 AGSSILAEFG TLHMEFVHLS YLTGDLTYYN KVMHIRKLLQ KMERPNGLYP NYLNPRTGRW 370 380 390 400 410 420 GQYHTSVGGL GDSFYEYLLK AWLMSDKTDH EARRMYDDAV EAIEKHLIKK SRGGLVFIGE 430 440 450 460 470 480 WKNGHLERKM GHLACFAGGM FALGADGSRK DKAGHYLELG AEIARTCHES YDRTALKLGP 490 500 510 520 530 540 ESFKFDGAVE AVAVRQAEKY YILRPEVIET YWYLWRFTHD PRYRQWGWEA ALAIEKSCRV 550 560 570 580 590 600 SGGFSGVKDV YAPTPVHDDV QQSFFLAETL KYLYLLFSGD DLLPLDHWVF NTEAHPLPVL 610 RLANSTLSGN PAVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a mouse Golgi mannosidase cDNA, a member of a gene family conserved from yeast to mammals." Herscovics A.A., Schneikert J., Athanassiadis A., Moremen K.W. J. Biol. Chem. 269:9864-9871(1994) [PubMed: 8144579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: BALB/c. Tissue: Fibroblast.
[2]	"Genomic organization and chromosomal mapping of the murine alpha 1,2-mannosidase IB involved in N-glycan maturation." Campbell-Dyke N., Athanassiadis A., Herscovics A. Genomics 41:155-159(1997) [PubMed: 9143489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Ola.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Bone marrow macrophage and Hippocampus.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NOD.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U03458 mRNA. Translation: AAB60439.1. U03457 mRNA. Translation: AAB60438.1. AF078095 , AF078083, AF078084, AF078085, AF078086, AF078087, AF078088, AF078089, AF078090, AF078091, AF078092, AF078093, AF078094 Genomic DNA. Translation: AAC34829.1. AK013515 mRNA. Translation: BAB28892.3. AK153336 mRNA. Translation: BAE31915.1. AL645760 Genomic DNA. Translation: CAI26213.1. AL645760 Genomic DNA. Translation: CAI26214.1. BC049121 mRNA. Translation: AAH49121.1. BC068192 mRNA. Translation: AAH68192.1.
IPI	IPI00131609. IPI00515213.
PIR	A54407.
RefSeq	NP_034893.1.
UniGene	Mm.271617 Mm.297043 Mm.458282
3D structure databases
HSSP	HSSP built from PDB template 1DL2 based on UniProtKB P32906.
ModBase	Search...
Protein-protein interaction databases
STRING	P39098.
Protein family/group databases
CAZy	GH47. Glycoside Hydrolase Family 47.
Proteomic databases
PRIDE	P39098.
Genome annotation databases
Ensembl	ENSMUST00000008907; ENSMUSP00000008907; ENSMUSG00000008763; Mus musculus. [Genome view] ENSMUST00000090730; ENSMUSP00000088232; ENSMUSG00000008763; Mus musculus. [Genome view]
GeneID	17156.
KEGG	mmu:17156.
UCSC	uc008qqw.1. mouse.
Organism-specific databases
CTD	17156.
MGI	MGI:104676. Man1a2.
Phylogenomic databases
HOGENOM	P39098.
HOVERGEN	P39098.
OMA	DHEARKM.
Enzyme and pathway databases
BRENDA	3.2.1.113. 244.
Gene expression databases
ArrayExpress	P39098.
Bgee	P39098.
CleanEx	MM_MAN1A2.
Genevestigator	P39098.
GermOnline	ENSMUSG00000008763. Mus musculus.
Family and domain databases
InterPro	IPR001382. Glyco_hydro_47. [Graphical view]
Gene3D	G3DSA:1.50.10.50. Glyco_hydro_47. 1 hit.
PANTHER	PTHR11742. Glyco_hydro_47. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
ProtoNet	Search...
Other Resources
NextBio	291422.
SOURCE	Search...

Entry information

Entry name

MA1A2_MOUSE

Accession

Primary (citable) accession number: P39098
Secondary accession number(s): Q5SUY6 , Q5SUY7, Q60599, Q9CUY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)