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P39098

- MA1A2_MOUSE

UniProt

P39098 - MA1A2_MOUSE

Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB

Gene

Man1a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

    Catalytic activityi

    Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

    Cofactori

    Calcium.

    Enzyme regulationi

    Inhibited by both 1-deoxymannojirimycin and kifunensine.By similarity

    Pathwayi

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycoprotein metabolic process Source: MGI
    2. lung alveolus development Source: MGI
    3. protein glycosylation Source: UniProtKB-UniPathway
    4. respiratory gaseous exchange Source: MGI

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    BRENDAi3.2.1.113. 3474.
    ReactomeiREACT_198545. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGH47. Glycoside Hydrolase Family 47.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC:3.2.1.113)
    Alternative name(s):
    Mannosidase alpha class 1A member 2
    Processing alpha-1,2-mannosidase IB
    Short name:
    Alpha-1,2-mannosidase IB
    Gene namesi
    Name:Man1a2
    Synonyms:Man1b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:104676. Man1a2.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: MGI
    2. integral component of membrane Source: UniProtKB-KW
    3. nucleolus Source: Ensembl

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 641640Mannosyl-oligosaccharide 1,2-alpha-mannosidase IBPRO_0000210313Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonineBy similarity
    Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Acetylation, Glycoprotein

    Proteomic databases

    MaxQBiP39098.
    PaxDbiP39098.
    PRIDEiP39098.

    PTM databases

    PhosphoSiteiP39098.

    Expressioni

    Gene expression databases

    ArrayExpressiP39098.
    BgeeiP39098.
    CleanExiMM_MAN1A2.
    GenevestigatoriP39098.

    Interactioni

    Protein-protein interaction databases

    BioGridi201303. 1 interaction.
    IntActiP39098. 1 interaction.
    MINTiMINT-4100907.

    Structurei

    3D structure databases

    ProteinModelPortaliP39098.
    SMRiP39098. Positions 175-626.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 3635CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini58 – 641584LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei37 – 5721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300315.
    GeneTreeiENSGT00390000016529.
    HOGENOMiHOG000181988.
    HOVERGENiHBG052389.
    InParanoidiP39098.
    KOiK01230.
    OMAiDPEDNDI.
    OrthoDBiEOG7X0VH1.
    PhylomeDBiP39098.
    TreeFamiTF313420.

    Family and domain databases

    Gene3Di1.50.10.50. 1 hit.
    InterProiIPR001382. Glyco_hydro_47.
    [Graphical view]
    PANTHERiPTHR11742. PTHR11742. 1 hit.
    PfamiPF01532. Glyco_hydro_47. 1 hit.
    [Graphical view]
    PRINTSiPR00747. GLYHDRLASE47.
    SUPFAMiSSF48225. SSF48225. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P39098-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC    50
    FGAFFFLPDS SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH 100
    REEEERLRNK IRADHEKALE EAKEKLRKSR EEIRAEIQTE KNKVAQAMKT 150
    KETRVLPPVP VPQRVGVSGG DPEDMEIKKK RDKIKEMMKH AWDNYRTYGW 200
    GHNELRPIAR KGHSTNIFGS SQMGATIVDA LDTLYIMGLH DEFMDGQRWI 250
    EENLDFSVNS EVSVFEVNIR FIGGLLAAYY LSGEEIFKTK AVQLAEKLLP 300
    AFNTPTGIPW AMVNLKSGVG RNWGWASAGS SILAEFGTLH MEFVHLSYLT 350
    GDLTYYNKVM HIRKLLQKME RPNGLYPNYL NPRTGRWGQY HTSVGGLGDS 400
    FYEYLLKAWL MSDKTDHEAR RMYDDAVEAI EKHLIKKSRG GLVFIGEWKN 450
    GHLERKMGHL ACFAGGMFAL GADGSRKDKA GHYLELGAEI ARTCHESYDR 500
    TALKLGPESF KFDGAVEAVA VRQAEKYYIL RPEVIETYWY LWRFTHDPRY 550
    RQWGWEAALA IEKSCRVSGG FSGVKDVYAP TPVHDDVQQS FFLAETLKYL 600
    YLLFSGDDLL PLDHWVFNTE AHPLPVLRLA NSTLSGNPAV R 641
    Length:641
    Mass (Da):72,871
    Last modified:February 1, 1995 - v1
    Checksum:i7747FE57CCB2C4C5
    GO
    Isoform 2 (identifier: P39098-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         259-285: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:614
    Mass (Da):69,912
    Checksum:i43E504696E83116B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti411 – 4111M → T in AAB60438. (PubMed:8144579)Curated
    Sequence conflicti468 – 4681F → L in AAB60438. (PubMed:8144579)Curated
    Sequence conflicti592 – 5921F → S in AAB60438. (PubMed:8144579)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei259 – 28527Missing in isoform 2. CuratedVSP_018615Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03458 mRNA. Translation: AAB60439.1.
    U03457 mRNA. Translation: AAB60438.1.
    AF078095
    , AF078083, AF078084, AF078085, AF078086, AF078087, AF078088, AF078089, AF078090, AF078091, AF078092, AF078093, AF078094 Genomic DNA. Translation: AAC34829.1.
    AK013515 mRNA. Translation: BAB28892.3.
    AK153336 mRNA. Translation: BAE31915.1.
    AL645760 Genomic DNA. Translation: CAI26213.1.
    AL645760 Genomic DNA. Translation: CAI26214.1.
    BC049121 mRNA. Translation: AAH49121.1.
    BC068192 mRNA. Translation: AAH68192.1.
    CCDSiCCDS17676.1. [P39098-1]
    PIRiA54407.
    RefSeqiNP_034893.1. NM_010763.2. [P39098-1]
    UniGeneiMm.271617.

    Genome annotation databases

    EnsembliENSMUST00000008907; ENSMUSP00000008907; ENSMUSG00000008763. [P39098-1]
    GeneIDi17156.
    KEGGimmu:17156.
    UCSCiuc008qqw.3. mouse. [P39098-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03458 mRNA. Translation: AAB60439.1 .
    U03457 mRNA. Translation: AAB60438.1 .
    AF078095
    , AF078083 , AF078084 , AF078085 , AF078086 , AF078087 , AF078088 , AF078089 , AF078090 , AF078091 , AF078092 , AF078093 , AF078094 Genomic DNA. Translation: AAC34829.1 .
    AK013515 mRNA. Translation: BAB28892.3 .
    AK153336 mRNA. Translation: BAE31915.1 .
    AL645760 Genomic DNA. Translation: CAI26213.1 .
    AL645760 Genomic DNA. Translation: CAI26214.1 .
    BC049121 mRNA. Translation: AAH49121.1 .
    BC068192 mRNA. Translation: AAH68192.1 .
    CCDSi CCDS17676.1. [P39098-1 ]
    PIRi A54407.
    RefSeqi NP_034893.1. NM_010763.2. [P39098-1 ]
    UniGenei Mm.271617.

    3D structure databases

    ProteinModelPortali P39098.
    SMRi P39098. Positions 175-626.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201303. 1 interaction.
    IntActi P39098. 1 interaction.
    MINTi MINT-4100907.

    Protein family/group databases

    CAZyi GH47. Glycoside Hydrolase Family 47.

    PTM databases

    PhosphoSitei P39098.

    Proteomic databases

    MaxQBi P39098.
    PaxDbi P39098.
    PRIDEi P39098.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000008907 ; ENSMUSP00000008907 ; ENSMUSG00000008763 . [P39098-1 ]
    GeneIDi 17156.
    KEGGi mmu:17156.
    UCSCi uc008qqw.3. mouse. [P39098-1 ]

    Organism-specific databases

    CTDi 10905.
    MGIi MGI:104676. Man1a2.

    Phylogenomic databases

    eggNOGi NOG300315.
    GeneTreei ENSGT00390000016529.
    HOGENOMi HOG000181988.
    HOVERGENi HBG052389.
    InParanoidi P39098.
    KOi K01230.
    OMAi DPEDNDI.
    OrthoDBi EOG7X0VH1.
    PhylomeDBi P39098.
    TreeFami TF313420.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BRENDAi 3.2.1.113. 3474.
    Reactomei REACT_198545. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.

    Miscellaneous databases

    NextBioi 291422.
    PROi P39098.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P39098.
    Bgeei P39098.
    CleanExi MM_MAN1A2.
    Genevestigatori P39098.

    Family and domain databases

    Gene3Di 1.50.10.50. 1 hit.
    InterProi IPR001382. Glyco_hydro_47.
    [Graphical view ]
    PANTHERi PTHR11742. PTHR11742. 1 hit.
    Pfami PF01532. Glyco_hydro_47. 1 hit.
    [Graphical view ]
    PRINTSi PR00747. GLYHDRLASE47.
    SUPFAMi SSF48225. SSF48225. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a mouse Golgi mannosidase cDNA, a member of a gene family conserved from yeast to mammals."
      Herscovics A.A., Schneikert J., Athanassiadis A., Moremen K.W.
      J. Biol. Chem. 269:9864-9871(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: BALB/c.
      Tissue: Fibroblast.
    2. "Genomic organization and chromosomal mapping of the murine alpha 1,2-mannosidase IB involved in N-glycan maturation."
      Campbell-Dyke N., Athanassiadis A., Herscovics A.
      Genomics 41:155-159(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Ola.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Bone marrow macrophage and Hippocampus.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NOD.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiMA1A2_MOUSE
    AccessioniPrimary (citable) accession number: P39098
    Secondary accession number(s): Q5SUY6
    , Q5SUY7, Q60599, Q9CUY9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3