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P39098

- MA1A2_MOUSE

UniProt

P39098 - MA1A2_MOUSE

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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB

Gene

Man1a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Calcium.

Enzyme regulationi

Inhibited by both 1-deoxymannojirimycin and kifunensine.By similarity

Pathwayi

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycoprotein metabolic process Source: MGI
  2. lung alveolus development Source: MGI
  3. protein glycosylation Source: UniProtKB-UniPathway
  4. respiratory gaseous exchange Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BRENDAi3.2.1.113. 3474.
ReactomeiREACT_198545. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC:3.2.1.113)
Alternative name(s):
Mannosidase alpha class 1A member 2
Processing alpha-1,2-mannosidase IB
Short name:
Alpha-1,2-mannosidase IB
Gene namesi
Name:Man1a2
Synonyms:Man1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:104676. Man1a2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. Golgi membrane Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. nucleolus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 641640Mannosyl-oligosaccharide 1,2-alpha-mannosidase IBPRO_0000210313Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

MaxQBiP39098.
PaxDbiP39098.
PRIDEiP39098.

PTM databases

PhosphoSiteiP39098.

Expressioni

Gene expression databases

BgeeiP39098.
CleanExiMM_MAN1A2.
ExpressionAtlasiP39098. baseline and differential.
GenevestigatoriP39098.

Interactioni

Protein-protein interaction databases

BioGridi201303. 1 interaction.
IntActiP39098. 1 interaction.
MINTiMINT-4100907.

Structurei

3D structure databases

ProteinModelPortaliP39098.
SMRiP39098. Positions 175-626.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3635CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini58 – 641584LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei37 – 5721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300315.
GeneTreeiENSGT00390000016529.
HOGENOMiHOG000181988.
HOVERGENiHBG052389.
InParanoidiP39098.
KOiK01230.
OMAiDPEDNDI.
OrthoDBiEOG7X0VH1.
PhylomeDBiP39098.
TreeFamiTF313420.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P39098-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC
60 70 80 90 100
FGAFFFLPDS SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH
110 120 130 140 150
REEEERLRNK IRADHEKALE EAKEKLRKSR EEIRAEIQTE KNKVAQAMKT
160 170 180 190 200
KETRVLPPVP VPQRVGVSGG DPEDMEIKKK RDKIKEMMKH AWDNYRTYGW
210 220 230 240 250
GHNELRPIAR KGHSTNIFGS SQMGATIVDA LDTLYIMGLH DEFMDGQRWI
260 270 280 290 300
EENLDFSVNS EVSVFEVNIR FIGGLLAAYY LSGEEIFKTK AVQLAEKLLP
310 320 330 340 350
AFNTPTGIPW AMVNLKSGVG RNWGWASAGS SILAEFGTLH MEFVHLSYLT
360 370 380 390 400
GDLTYYNKVM HIRKLLQKME RPNGLYPNYL NPRTGRWGQY HTSVGGLGDS
410 420 430 440 450
FYEYLLKAWL MSDKTDHEAR RMYDDAVEAI EKHLIKKSRG GLVFIGEWKN
460 470 480 490 500
GHLERKMGHL ACFAGGMFAL GADGSRKDKA GHYLELGAEI ARTCHESYDR
510 520 530 540 550
TALKLGPESF KFDGAVEAVA VRQAEKYYIL RPEVIETYWY LWRFTHDPRY
560 570 580 590 600
RQWGWEAALA IEKSCRVSGG FSGVKDVYAP TPVHDDVQQS FFLAETLKYL
610 620 630 640
YLLFSGDDLL PLDHWVFNTE AHPLPVLRLA NSTLSGNPAV R
Length:641
Mass (Da):72,871
Last modified:February 1, 1995 - v1
Checksum:i7747FE57CCB2C4C5
GO
Isoform 2 (identifier: P39098-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-285: Missing.

Note: No experimental confirmation available.

Show »
Length:614
Mass (Da):69,912
Checksum:i43E504696E83116B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti411 – 4111M → T in AAB60438. (PubMed:8144579)Curated
Sequence conflicti468 – 4681F → L in AAB60438. (PubMed:8144579)Curated
Sequence conflicti592 – 5921F → S in AAB60438. (PubMed:8144579)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei259 – 28527Missing in isoform 2. CuratedVSP_018615Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03458 mRNA. Translation: AAB60439.1.
U03457 mRNA. Translation: AAB60438.1.
AF078095
, AF078083, AF078084, AF078085, AF078086, AF078087, AF078088, AF078089, AF078090, AF078091, AF078092, AF078093, AF078094 Genomic DNA. Translation: AAC34829.1.
AK013515 mRNA. Translation: BAB28892.3.
AK153336 mRNA. Translation: BAE31915.1.
AL645760 Genomic DNA. Translation: CAI26213.1.
AL645760 Genomic DNA. Translation: CAI26214.1.
BC049121 mRNA. Translation: AAH49121.1.
BC068192 mRNA. Translation: AAH68192.1.
CCDSiCCDS17676.1. [P39098-1]
PIRiA54407.
RefSeqiNP_034893.1. NM_010763.2. [P39098-1]
UniGeneiMm.271617.

Genome annotation databases

EnsembliENSMUST00000008907; ENSMUSP00000008907; ENSMUSG00000008763. [P39098-1]
GeneIDi17156.
KEGGimmu:17156.
UCSCiuc008qqw.3. mouse. [P39098-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03458 mRNA. Translation: AAB60439.1 .
U03457 mRNA. Translation: AAB60438.1 .
AF078095
, AF078083 , AF078084 , AF078085 , AF078086 , AF078087 , AF078088 , AF078089 , AF078090 , AF078091 , AF078092 , AF078093 , AF078094 Genomic DNA. Translation: AAC34829.1 .
AK013515 mRNA. Translation: BAB28892.3 .
AK153336 mRNA. Translation: BAE31915.1 .
AL645760 Genomic DNA. Translation: CAI26213.1 .
AL645760 Genomic DNA. Translation: CAI26214.1 .
BC049121 mRNA. Translation: AAH49121.1 .
BC068192 mRNA. Translation: AAH68192.1 .
CCDSi CCDS17676.1. [P39098-1 ]
PIRi A54407.
RefSeqi NP_034893.1. NM_010763.2. [P39098-1 ]
UniGenei Mm.271617.

3D structure databases

ProteinModelPortali P39098.
SMRi P39098. Positions 175-626.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201303. 1 interaction.
IntActi P39098. 1 interaction.
MINTi MINT-4100907.

Protein family/group databases

CAZyi GH47. Glycoside Hydrolase Family 47.

PTM databases

PhosphoSitei P39098.

Proteomic databases

MaxQBi P39098.
PaxDbi P39098.
PRIDEi P39098.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000008907 ; ENSMUSP00000008907 ; ENSMUSG00000008763 . [P39098-1 ]
GeneIDi 17156.
KEGGi mmu:17156.
UCSCi uc008qqw.3. mouse. [P39098-1 ]

Organism-specific databases

CTDi 10905.
MGIi MGI:104676. Man1a2.

Phylogenomic databases

eggNOGi NOG300315.
GeneTreei ENSGT00390000016529.
HOGENOMi HOG000181988.
HOVERGENi HBG052389.
InParanoidi P39098.
KOi K01230.
OMAi DPEDNDI.
OrthoDBi EOG7X0VH1.
PhylomeDBi P39098.
TreeFami TF313420.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 3.2.1.113. 3474.
Reactomei REACT_198545. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.

Miscellaneous databases

NextBioi 291422.
PROi P39098.
SOURCEi Search...

Gene expression databases

Bgeei P39098.
CleanExi MM_MAN1A2.
ExpressionAtlasi P39098. baseline and differential.
Genevestigatori P39098.

Family and domain databases

Gene3Di 1.50.10.50. 1 hit.
InterProi IPR001382. Glyco_hydro_47.
[Graphical view ]
PANTHERi PTHR11742. PTHR11742. 1 hit.
Pfami PF01532. Glyco_hydro_47. 1 hit.
[Graphical view ]
PRINTSi PR00747. GLYHDRLASE47.
SUPFAMi SSF48225. SSF48225. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a mouse Golgi mannosidase cDNA, a member of a gene family conserved from yeast to mammals."
    Herscovics A.A., Schneikert J., Athanassiadis A., Moremen K.W.
    J. Biol. Chem. 269:9864-9871(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/c.
    Tissue: Fibroblast.
  2. "Genomic organization and chromosomal mapping of the murine alpha 1,2-mannosidase IB involved in N-glycan maturation."
    Campbell-Dyke N., Athanassiadis A., Herscovics A.
    Genomics 41:155-159(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Ola.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Bone marrow macrophage and Hippocampus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NOD.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiMA1A2_MOUSE
AccessioniPrimary (citable) accession number: P39098
Secondary accession number(s): Q5SUY6
, Q5SUY7, Q60599, Q9CUY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3