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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB

Gene

Man1a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity

Enzyme regulationi

Inhibited by both 1-deoxymannojirimycin and kifunensine.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei508Proton donorBy similarity1
Metal bindingi619CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glycoprotein metabolic process Source: MGI
  • lung alveolus development Source: MGI
  • N-glycan processing Source: GO_Central
  • respiratory gaseous exchange Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.2.1.113. 3474.
ReactomeiR-MMU-964827. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC:3.2.1.113By similarity)
Alternative name(s):
Mannosidase alpha class 1A member 2
Processing alpha-1,2-mannosidase IB
Short name:
Alpha-1,2-mannosidase IB
Gene namesi
Name:Man1a2
Synonyms:Man1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:104676. Man1a2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 36CytoplasmicSequence analysisAdd BLAST35
Transmembranei37 – 57Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini58 – 641LumenalSequence analysisAdd BLAST584

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002103132 – 641Mannosyl-oligosaccharide 1,2-alpha-mannosidase IBAdd BLAST640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Disulfide bondi462 ↔ 494By similarity
Glycosylationi631N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP39098.
PaxDbiP39098.
PeptideAtlasiP39098.
PRIDEiP39098.

PTM databases

iPTMnetiP39098.
PhosphoSitePlusiP39098.

Expressioni

Gene expression databases

BgeeiENSMUSG00000008763.
CleanExiMM_MAN1A2.
ExpressionAtlasiP39098. baseline and differential.
GenevisibleiP39098. MM.

Interactioni

Protein-protein interaction databases

BioGridi201303. 1 interactor.
IntActiP39098. 1 interactor.
MINTiMINT-4100907.
STRINGi10090.ENSMUSP00000008907.

Structurei

3D structure databases

ProteinModelPortaliP39098.
SMRiP39098.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2431. Eukaryota.
ENOG410XP04. LUCA.
GeneTreeiENSGT00390000016529.
HOGENOMiHOG000181988.
HOVERGENiHBG052389.
InParanoidiP39098.
KOiK01230.
OMAiHEARRMY.
OrthoDBiEOG091G04C8.
PhylomeDBiP39098.
TreeFamiTF313420.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P39098-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC
60 70 80 90 100
FGAFFFLPDS SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH
110 120 130 140 150
REEEERLRNK IRADHEKALE EAKEKLRKSR EEIRAEIQTE KNKVAQAMKT
160 170 180 190 200
KETRVLPPVP VPQRVGVSGG DPEDMEIKKK RDKIKEMMKH AWDNYRTYGW
210 220 230 240 250
GHNELRPIAR KGHSTNIFGS SQMGATIVDA LDTLYIMGLH DEFMDGQRWI
260 270 280 290 300
EENLDFSVNS EVSVFEVNIR FIGGLLAAYY LSGEEIFKTK AVQLAEKLLP
310 320 330 340 350
AFNTPTGIPW AMVNLKSGVG RNWGWASAGS SILAEFGTLH MEFVHLSYLT
360 370 380 390 400
GDLTYYNKVM HIRKLLQKME RPNGLYPNYL NPRTGRWGQY HTSVGGLGDS
410 420 430 440 450
FYEYLLKAWL MSDKTDHEAR RMYDDAVEAI EKHLIKKSRG GLVFIGEWKN
460 470 480 490 500
GHLERKMGHL ACFAGGMFAL GADGSRKDKA GHYLELGAEI ARTCHESYDR
510 520 530 540 550
TALKLGPESF KFDGAVEAVA VRQAEKYYIL RPEVIETYWY LWRFTHDPRY
560 570 580 590 600
RQWGWEAALA IEKSCRVSGG FSGVKDVYAP TPVHDDVQQS FFLAETLKYL
610 620 630 640
YLLFSGDDLL PLDHWVFNTE AHPLPVLRLA NSTLSGNPAV R
Length:641
Mass (Da):72,871
Last modified:February 1, 1995 - v1
Checksum:i7747FE57CCB2C4C5
GO
Isoform 2 (identifier: P39098-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-285: Missing.

Note: No experimental confirmation available.
Show »
Length:614
Mass (Da):69,912
Checksum:i43E504696E83116B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti411M → T in AAB60438 (PubMed:8144579).Curated1
Sequence conflicti468F → L in AAB60438 (PubMed:8144579).Curated1
Sequence conflicti592F → S in AAB60438 (PubMed:8144579).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018615259 – 285Missing in isoform 2. CuratedAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03458 mRNA. Translation: AAB60439.1.
U03457 mRNA. Translation: AAB60438.1.
AF078095
, AF078083, AF078084, AF078085, AF078086, AF078087, AF078088, AF078089, AF078090, AF078091, AF078092, AF078093, AF078094 Genomic DNA. Translation: AAC34829.1.
AK013515 mRNA. Translation: BAB28892.3.
AK153336 mRNA. Translation: BAE31915.1.
AL645760 Genomic DNA. Translation: CAI26213.1.
AL645760 Genomic DNA. Translation: CAI26214.1.
BC049121 mRNA. Translation: AAH49121.1.
BC068192 mRNA. Translation: AAH68192.1.
CCDSiCCDS17676.1. [P39098-1]
PIRiA54407.
RefSeqiNP_034893.1. NM_010763.2. [P39098-1]
UniGeneiMm.271617.

Genome annotation databases

EnsembliENSMUST00000008907; ENSMUSP00000008907; ENSMUSG00000008763. [P39098-1]
GeneIDi17156.
KEGGimmu:17156.
UCSCiuc008qqw.3. mouse. [P39098-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03458 mRNA. Translation: AAB60439.1.
U03457 mRNA. Translation: AAB60438.1.
AF078095
, AF078083, AF078084, AF078085, AF078086, AF078087, AF078088, AF078089, AF078090, AF078091, AF078092, AF078093, AF078094 Genomic DNA. Translation: AAC34829.1.
AK013515 mRNA. Translation: BAB28892.3.
AK153336 mRNA. Translation: BAE31915.1.
AL645760 Genomic DNA. Translation: CAI26213.1.
AL645760 Genomic DNA. Translation: CAI26214.1.
BC049121 mRNA. Translation: AAH49121.1.
BC068192 mRNA. Translation: AAH68192.1.
CCDSiCCDS17676.1. [P39098-1]
PIRiA54407.
RefSeqiNP_034893.1. NM_010763.2. [P39098-1]
UniGeneiMm.271617.

3D structure databases

ProteinModelPortaliP39098.
SMRiP39098.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201303. 1 interactor.
IntActiP39098. 1 interactor.
MINTiMINT-4100907.
STRINGi10090.ENSMUSP00000008907.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

PTM databases

iPTMnetiP39098.
PhosphoSitePlusiP39098.

Proteomic databases

MaxQBiP39098.
PaxDbiP39098.
PeptideAtlasiP39098.
PRIDEiP39098.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008907; ENSMUSP00000008907; ENSMUSG00000008763. [P39098-1]
GeneIDi17156.
KEGGimmu:17156.
UCSCiuc008qqw.3. mouse. [P39098-1]

Organism-specific databases

CTDi10905.
MGIiMGI:104676. Man1a2.

Phylogenomic databases

eggNOGiKOG2431. Eukaryota.
ENOG410XP04. LUCA.
GeneTreeiENSGT00390000016529.
HOGENOMiHOG000181988.
HOVERGENiHBG052389.
InParanoidiP39098.
KOiK01230.
OMAiHEARRMY.
OrthoDBiEOG091G04C8.
PhylomeDBiP39098.
TreeFamiTF313420.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi3.2.1.113. 3474.
ReactomeiR-MMU-964827. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.

Miscellaneous databases

ChiTaRSiMan1a2. mouse.
PROiP39098.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008763.
CleanExiMM_MAN1A2.
ExpressionAtlasiP39098. baseline and differential.
GenevisibleiP39098. MM.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMA1A2_MOUSE
AccessioniPrimary (citable) accession number: P39098
Secondary accession number(s): Q5SUY6
, Q5SUY7, Q60599, Q9CUY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.