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P39098 (MA1A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB
EC=3.2.1.113
Alternative name(s):
Mannosidase alpha class 1A member 2
Processing alpha-1,2-mannosidase IB
Short name=Alpha-1,2-mannosidase IB
Gene names
Name:Man1a2
Synonyms:Man1b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length641 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activity

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactor

Calcium.

Enzyme regulation

Inhibited by both 1-deoxymannojirimycin and kifunensine By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P39098-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P39098-2)

The sequence of this isoform differs from the canonical sequence as follows:
     259-285: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 641641Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB
PRO_0000210313

Regions

Topological domain1 – 3636Cytoplasmic Potential
Transmembrane37 – 5721Helical; Signal-anchor for type II membrane protein; Potential
Topological domain58 – 641584Lumenal Potential

Amino acid modifications

Glycosylation6311N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence259 – 28527Missing in isoform 2.
VSP_018615

Experimental info

Sequence conflict4111M → T in AAB60438. Ref.1
Sequence conflict4681F → L in AAB60438. Ref.1
Sequence conflict5921F → S in AAB60438. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 7747FE57CCB2C4C5

FASTA64172,871
        10         20         30         40         50         60 
MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC FGAFFFLPDS 

        70         80         90        100        110        120 
SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH REEEERLRNK IRADHEKALE 

       130        140        150        160        170        180 
EAKEKLRKSR EEIRAEIQTE KNKVAQAMKT KETRVLPPVP VPQRVGVSGG DPEDMEIKKK 

       190        200        210        220        230        240 
RDKIKEMMKH AWDNYRTYGW GHNELRPIAR KGHSTNIFGS SQMGATIVDA LDTLYIMGLH 

       250        260        270        280        290        300 
DEFMDGQRWI EENLDFSVNS EVSVFEVNIR FIGGLLAAYY LSGEEIFKTK AVQLAEKLLP 

       310        320        330        340        350        360 
AFNTPTGIPW AMVNLKSGVG RNWGWASAGS SILAEFGTLH MEFVHLSYLT GDLTYYNKVM 

       370        380        390        400        410        420 
HIRKLLQKME RPNGLYPNYL NPRTGRWGQY HTSVGGLGDS FYEYLLKAWL MSDKTDHEAR 

       430        440        450        460        470        480 
RMYDDAVEAI EKHLIKKSRG GLVFIGEWKN GHLERKMGHL ACFAGGMFAL GADGSRKDKA 

       490        500        510        520        530        540 
GHYLELGAEI ARTCHESYDR TALKLGPESF KFDGAVEAVA VRQAEKYYIL RPEVIETYWY 

       550        560        570        580        590        600 
LWRFTHDPRY RQWGWEAALA IEKSCRVSGG FSGVKDVYAP TPVHDDVQQS FFLAETLKYL 

       610        620        630        640 
YLLFSGDDLL PLDHWVFNTE AHPLPVLRLA NSTLSGNPAV R

« Hide

Isoform 2 [UniParc].

Checksum: 43E504696E83116B
Show »

FASTA61469,912

References

« Hide 'large scale' references
[1]"Isolation of a mouse Golgi mannosidase cDNA, a member of a gene family conserved from yeast to mammals."
Herscovics A.A., Schneikert J., Athanassiadis A., Moremen K.W.
J. Biol. Chem. 269:9864-9871(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: BALB/c.
Tissue: Fibroblast.
[2]"Genomic organization and chromosomal mapping of the murine alpha 1,2-mannosidase IB involved in N-glycan maturation."
Campbell-Dyke N., Athanassiadis A., Herscovics A.
Genomics 41:155-159(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Ola.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Bone marrow macrophage and Hippocampus.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NOD.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U03458 mRNA. Translation: AAB60439.1.
U03457 mRNA. Translation: AAB60438.1.
AF078095 expand/collapse EMBL AC list , AF078083, AF078084, AF078085, AF078086, AF078087, AF078088, AF078089, AF078090, AF078091, AF078092, AF078093, AF078094 Genomic DNA. Translation: AAC34829.1.
AK013515 mRNA. Translation: BAB28892.3.
AK153336 mRNA. Translation: BAE31915.1.
AL645760 Genomic DNA. Translation: CAI26213.1.
AL645760 Genomic DNA. Translation: CAI26214.1.
BC049121 mRNA. Translation: AAH49121.1.
BC068192 mRNA. Translation: AAH68192.1.
IPIIPI00131609.
IPI00515213.
PIRA54407.
RefSeqNP_034893.1. NM_010763.2.
UniGeneMm.271617.

3D structure databases

ProteinModelPortalP39098.
SMRP39098. Positions 175-626.
ModBaseSearch...

Protein family/group databases

CAZyGH47. Glycoside Hydrolase Family 47.

PTM databases

PhosphoSiteP39098.

Proteomic databases

PaxDbP39098.
PRIDEP39098.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000008907; ENSMUSP00000008907; ENSMUSG00000008763.
GeneID17156.
KEGGmmu:17156.

Organism-specific databases

CTD10905.
MGIMGI:104676. Man1a2.

Phylogenomic databases

eggNOGNOG300315.
GeneTreeENSGT00390000016529.
HOGENOMHOG000181988.
HOVERGENHBG052389.
InParanoidP39098.
KOK01230.
OMANKVVQEM.
OrthoDBEOG4NVZJW.

Enzyme and pathway databases

BRENDA3.2.1.113. 3474.
UniPathwayUPA00378.

Gene expression databases

ArrayExpressP39098.
BgeeP39098.
CleanExMM_MAN1A2.
GenevestigatorP39098.
GermOnlineENSMUSG00000008763. Mus musculus.

Family and domain databases

Gene3D1.50.10.50. 1 hit.
InterProIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERPTHR11742. PTHR11742. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
SUPFAMSSF48225. Glyco_hydro_47. 1 hit.
ProtoNetSearch...

Other

NextBio291422.
SOURCESearch...

Entry information

Entry nameMA1A2_MOUSE
AccessionPrimary (citable) accession number: P39098
Secondary accession number(s): Q5SUY6 expand/collapse secondary AC list , Q5SUY7, Q60599, Q9CUY9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents