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Protein

Glutamate receptor ionotropic, kainate 1

Gene

GRIK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei538GlutamateBy similarity1
Binding sitei753GlutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • central nervous system development Source: ProtInc
  • chemical synaptic transmission Source: ProtInc
  • glutamate receptor signaling pathway Source: ProtInc
  • nervous system development Source: ProtInc
  • regulation of synaptic transmission, glutamatergic Source: UniProtKB
  • transport Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000171189-MONOMER.
ReactomeiR-HSA-451307. Activation of Na-permeable Kainate Receptors.
R-HSA-451308. Activation of Ca-permeable Kainate Receptor.
SignaLinkiP39086.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, kainate 1
Short name:
GluK1
Alternative name(s):
Excitatory amino acid receptor 3
Short name:
EAA3
Glutamate receptor 5
Short name:
GluR-5
Short name:
GluR5
Gene namesi
Name:GRIK1
Synonyms:GLUR5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

HGNCiHGNC:4579. GRIK1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 576ExtracellularSequence analysisAdd BLAST546
Transmembranei577 – 597HelicalSequence analysisAdd BLAST21
Topological domaini598 – 653CytoplasmicSequence analysisAdd BLAST56
Transmembranei654 – 674HelicalSequence analysisAdd BLAST21
Topological domaini675 – 834ExtracellularSequence analysisAdd BLAST160
Transmembranei835 – 855HelicalSequence analysisAdd BLAST21
Topological domaini856 – 918CytoplasmicSequence analysisAdd BLAST63

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: Reactome
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi2897.
OpenTargetsiENSG00000171189.
PharmGKBiPA28973.

Chemistry databases

ChEMBLiCHEMBL1918.
DrugBankiDB00273. Topiramate.
GuidetoPHARMACOLOGYi450.

Polymorphism and mutation databases

BioMutaiGRIK1.
DMDMi729597.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000001154131 – 918Glutamate receptor ionotropic, kainate 1Add BLAST888

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi68N-linked (GlcNAc...)Sequence analysis1
Glycosylationi74N-linked (GlcNAc...)Sequence analysis1
Glycosylationi276N-linked (GlcNAc...)Sequence analysis1
Glycosylationi379N-linked (GlcNAc...)Sequence analysis1
Glycosylationi428N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)Sequence analysis1
Glycosylationi446N-linked (GlcNAc...)Sequence analysis1
Glycosylationi561N-linked (GlcNAc...)Sequence analysis1
Modified residuei725Phosphoserine; by PKCSequence analysis1
Modified residuei761Phosphothreonine; by PKCSequence analysis1
Modified residuei861PhosphoserineBy similarity1
Cross-linki911Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP39086.
PeptideAtlasiP39086.
PRIDEiP39086.

PTM databases

iPTMnetiP39086.
PhosphoSitePlusiP39086.

Expressioni

Tissue specificityi

Most abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus.

Gene expression databases

BgeeiENSG00000171189.
CleanExiHS_GRIK1.
ExpressionAtlasiP39086. baseline and differential.
GenevisibleiP39086. HS.

Organism-specific databases

HPAiHPA014623.

Interactioni

Subunit structurei

Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers (Probable). The unedited version (Q) assembles into a functional kainate-gated homomeric channel, whereas the edited version (R) is unable to produce channel activity when expressed alone. Both edited and unedited versions can form functional channels with GRIK4 and GRIK5. Interacts with KLHL17 (By similarity).By similarityCurated

Protein-protein interaction databases

BioGridi109154. 4 interactors.
STRINGi9606.ENSP00000382791.

Chemistry databases

BindingDBiP39086.

Structurei

Secondary structure

1918
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi449 – 453Combined sources5
Turni457 – 459Combined sources3
Beta strandi460 – 462Combined sources3
Helixi471 – 474Combined sources4
Beta strandi475 – 477Combined sources3
Helixi478 – 490Combined sources13
Beta strandi494 – 498Combined sources5
Helixi515 – 521Combined sources7
Beta strandi526 – 528Combined sources3
Helixi536 – 539Combined sources4
Beta strandi542 – 544Combined sources3
Beta strandi548 – 551Combined sources4
Beta strandi553 – 559Combined sources7
Helixi686 – 691Combined sources6
Beta strandi693 – 698Combined sources6
Helixi704 – 711Combined sources8
Helixi715 – 726Combined sources12
Helixi728 – 731Combined sources4
Beta strandi732 – 735Combined sources4
Helixi736 – 745Combined sources10
Beta strandi746 – 753Combined sources8
Helixi754 – 761Combined sources8
Beta strandi767 – 771Combined sources5
Beta strandi777 – 779Combined sources3
Beta strandi782 – 784Combined sources3
Helixi790 – 802Combined sources13
Helixi805 – 814Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZNSX-ray2.00A445-559[»]
A682-820[»]
2ZNTX-ray1.60A445-559[»]
A682-820[»]
2ZNUX-ray1.80A445-559[»]
A682-820[»]
3FUZX-ray1.65A/B445-559[»]
A/B682-820[»]
3FV1X-ray1.50A/B445-559[»]
A/B682-820[»]
3FV2X-ray1.50A/B445-559[»]
A/B682-820[»]
3FVGX-ray1.50A/B445-559[»]
A/B682-820[»]
3FVKX-ray1.50A/B445-559[»]
A/B682-820[»]
3FVNX-ray1.50A/B445-559[»]
A/B682-820[»]
3FVOX-ray1.50A/B445-559[»]
A/B682-820[»]
4MF3X-ray3.00A/B443-559[»]
A/B682-822[»]
ProteinModelPortaliP39086.
SMRiP39086.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39086.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni531 – 533Glutamate bindingBy similarity3
Regioni704 – 705Glutamate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1054. Eukaryota.
ENOG410XPSH. LUCA.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000234371.
HOVERGENiHBG051839.
InParanoidiP39086.
KOiK05201.
PhylomeDBiP39086.
TreeFamiTF334668.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P39086-1) [UniParc]FASTAAdd to basket
Also known as: GluR5-1D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEHGTLLAQP GLWTRDTSWA LLYFLCYILP QTAPQVLRIG GIFETVENEP
60 70 80 90 100
VNVEELAFKF AVTSINRNRT LMPNTTLTYD IQRINLFDSF EASRRACDQL
110 120 130 140 150
ALGVAALFGP SHSSSVSAVQ SICNALEVPH IQTRWKHPSV DNKDLFYINL
160 170 180 190 200
YPDYAAISRA ILDLVLYYNW KTVTVVYEDS TGLIRLQELI KAPSRYNIKI
210 220 230 240 250
KIRQLPSGNK DAKPLLKEMK KGKEFYVIFD CSHETAAEIL KQILFMGMMT
260 270 280 290 300
EYYHYFFTTL DLFALDLELY RYSGVNMTGF RLLNIDNPHV SSIIEKWSME
310 320 330 340 350
RLQAPPRPET GLLDGMMTTE AALMYDAVYM VAIASHRASQ LTVSSLQCHR
360 370 380 390 400
HKPWRLGPRF MNLIKEARWD GLTGHITFNK TNGLRKDFDL DIISLKEEGT
410 420 430 440 450
EKAAGEVSKH LYKVWKKIGI WNSNSGLNMT DSNKDKSSNI TDSLANRTLI
460 470 480 490 500
VTTILEEPYV MYRKSDKPLY GNDRFEGYCL DLLKELSNIL GFIYDVKLVP
510 520 530 540 550
DGKYGAQNDK GEWNGMVKEL IDHRADLAVA PLTITYVREK VIDFSKPFMT
560 570 580 590 600
LGISILYRKP NGTNPGVFSF LNPLSPDIWM YVLLACLGVS CVLFVIARFT
610 620 630 640 650
PYEWYNPHPC NPDSDVVENN FTLLNSFWFG VGALMQQGSE LMPKALSTRI
660 670 680 690 700
VGGIWWFFTL IIISSYTANL AAFLTVERME SPIDSADDLA KQTKIEYGAV
710 720 730 740 750
RDGSTMTFFK KSKISTYEKM WAFMSSRQQT ALVRNSDEGI QRVLTTDYAL
760 770 780 790 800
LMESTSIEYV TQRNCNLTQI GGLIDSKGYG VGTPIGSPYR DKITIAILQL
810 820 830 840 850
QEEGKLHMMK EKWWRGNGCP EEDNKEASAL GVENIGGIFI VLAAGLVLSV
860 870 880 890 900
FVAIGEFIYK SRKNNDIEQA FCFFYGLQCK QTHPTNSTSG TTLSTDLECG
910
KLIREERGIR KQSSVHTV
Length:918
Mass (Da):103,981
Last modified:February 1, 1995 - v1
Checksum:i0EB8DB6356599002
GO
Isoform 2 (identifier: P39086-2) [UniParc]FASTAAdd to basket
Also known as: EAA3A

The sequence of this isoform differs from the canonical sequence as follows:
     402-416: Missing.
     870-918: AFCFFYGLQC...IRKQSSVHTV → CLSFNAIMEE...RRTQRKETVA

Show »
Length:905
Mass (Da):102,631
Checksum:i6D9350206E5A2E9C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281R → G in AAA95961 (PubMed:8589992).Curated1

RNA editingi

Edited at position 636.1 Publication
Partially edited.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_012751332A → V.1 PublicationCorresponds to variant rs143252117dbSNPEnsembl.1
Natural variantiVAR_000304636Q → R in RNA edited version. 1
Natural variantiVAR_012041757I → V.Corresponds to variant rs363494dbSNPEnsembl.1
Natural variantiVAR_012752862R → Q.1 PublicationCorresponds to variant rs761410270dbSNPEnsembl.1
Natural variantiVAR_012042870A → V.Corresponds to variant rs363503dbSNPEnsembl.1
Natural variantiVAR_012043902L → S.1 PublicationCorresponds to variant rs363504dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000127402 – 416Missing in isoform 2. 2 PublicationsAdd BLAST15
Alternative sequenceiVSP_000128870 – 918AFCFF…SVHTV → CLSFNAIMEELGISLKNQKK IKKKSRTKGKSSFTSILTCH QRRTQRKETVA in isoform 2. 2 PublicationsAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19058 mRNA. Translation: AAA52568.1.
U16125 mRNA. Translation: AAA95961.1.
AJ249208 mRNA. Translation: CAC80546.1.
CCDSiCCDS33530.1. [P39086-2]
CCDS42913.1. [P39086-1]
PIRiI58178.
RefSeqiNP_000821.1. NM_000830.4. [P39086-1]
NP_001307545.1. NM_001320616.1.
NP_001307547.1. NM_001320618.1.
NP_783300.1. NM_175611.2. [P39086-2]
UniGeneiHs.664641.
Hs.670516.

Genome annotation databases

EnsembliENST00000389125; ENSP00000373777; ENSG00000171189. [P39086-2]
ENST00000399907; ENSP00000382791; ENSG00000171189. [P39086-1]
GeneIDi2897.
KEGGihsa:2897.
UCSCiuc002ynn.4. human. [P39086-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism, RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19058 mRNA. Translation: AAA52568.1.
U16125 mRNA. Translation: AAA95961.1.
AJ249208 mRNA. Translation: CAC80546.1.
CCDSiCCDS33530.1. [P39086-2]
CCDS42913.1. [P39086-1]
PIRiI58178.
RefSeqiNP_000821.1. NM_000830.4. [P39086-1]
NP_001307545.1. NM_001320616.1.
NP_001307547.1. NM_001320618.1.
NP_783300.1. NM_175611.2. [P39086-2]
UniGeneiHs.664641.
Hs.670516.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZNSX-ray2.00A445-559[»]
A682-820[»]
2ZNTX-ray1.60A445-559[»]
A682-820[»]
2ZNUX-ray1.80A445-559[»]
A682-820[»]
3FUZX-ray1.65A/B445-559[»]
A/B682-820[»]
3FV1X-ray1.50A/B445-559[»]
A/B682-820[»]
3FV2X-ray1.50A/B445-559[»]
A/B682-820[»]
3FVGX-ray1.50A/B445-559[»]
A/B682-820[»]
3FVKX-ray1.50A/B445-559[»]
A/B682-820[»]
3FVNX-ray1.50A/B445-559[»]
A/B682-820[»]
3FVOX-ray1.50A/B445-559[»]
A/B682-820[»]
4MF3X-ray3.00A/B443-559[»]
A/B682-822[»]
ProteinModelPortaliP39086.
SMRiP39086.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109154. 4 interactors.
STRINGi9606.ENSP00000382791.

Chemistry databases

BindingDBiP39086.
ChEMBLiCHEMBL1918.
DrugBankiDB00273. Topiramate.
GuidetoPHARMACOLOGYi450.

PTM databases

iPTMnetiP39086.
PhosphoSitePlusiP39086.

Polymorphism and mutation databases

BioMutaiGRIK1.
DMDMi729597.

Proteomic databases

PaxDbiP39086.
PeptideAtlasiP39086.
PRIDEiP39086.

Protocols and materials databases

DNASUi2897.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000389125; ENSP00000373777; ENSG00000171189. [P39086-2]
ENST00000399907; ENSP00000382791; ENSG00000171189. [P39086-1]
GeneIDi2897.
KEGGihsa:2897.
UCSCiuc002ynn.4. human. [P39086-1]

Organism-specific databases

CTDi2897.
DisGeNETi2897.
GeneCardsiGRIK1.
HGNCiHGNC:4579. GRIK1.
HPAiHPA014623.
MIMi138245. gene.
neXtProtiNX_P39086.
OpenTargetsiENSG00000171189.
PharmGKBiPA28973.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1054. Eukaryota.
ENOG410XPSH. LUCA.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000234371.
HOVERGENiHBG051839.
InParanoidiP39086.
KOiK05201.
PhylomeDBiP39086.
TreeFamiTF334668.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000171189-MONOMER.
ReactomeiR-HSA-451307. Activation of Na-permeable Kainate Receptors.
R-HSA-451308. Activation of Ca-permeable Kainate Receptor.
SignaLinkiP39086.

Miscellaneous databases

ChiTaRSiGRIK1. human.
EvolutionaryTraceiP39086.
GeneWikiiGRIK1.
GenomeRNAii2897.
PROiP39086.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171189.
CleanExiHS_GRIK1.
ExpressionAtlasiP39086. baseline and differential.
GenevisibleiP39086. HS.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGRIK1_HUMAN
AccessioniPrimary (citable) accession number: P39086
Secondary accession number(s): Q13001, Q86SU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 167 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate > L-glutamate = quisqualate > CNQX = DNQX > AMPA > dihydrokainate > NMDA.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.