T-complex protein 1 subunit beta - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P39076 (TCPB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
T-complex protein 1 subunit beta
Short name=TCP-1-beta

Alternative name(s):
CCT-beta

Gene names

Name:	CCT2
Synonyms:	BIN3, TCP2
Ordered Locus Names:	YIL142W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.
Subunit structure	Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the TCP-1 chaperonin family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from direct assay PubMed 16762366. Source: SGD
Cellular_component	chaperonin-containing T-complex Inferred from direct assay PubMed 16762366. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW unfolded protein binding Inferred from direct assay PubMed 16762366. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 527

527

T-complex protein 1 subunit beta

PRO_0000128320

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphothreonine Ref.8

Modified residue

254

Phosphoserine Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P39076 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: C5DB2BC3660EC7E6
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FASTA

527

57,203

        10         20         30         40         50         60 
MSVQIFGDQV TEERAENARL SAFVGAIAVG DLVKSTLGPK GMDKLLQSAS SNTCMVTNDG 

        70         80         90        100        110        120 
ATILKSIPLD NPAAKVLVNI SKVQDDEVGD GTTSVTVLSA ELLREAEKLI DQSKIHPQTI 

       130        140        150        160        170        180 
IEGYRLASAA ALDALTKAAV DNSHDKTMFR EDLIHIAKTT LSSKILSQDK DHFAELATNA 

       190        200        210        220        230        240 
ILRLKGSTNL EHIQIIKILG GKLSDSFLDE GFILAKKFGN NQPKRIENAK ILIANTTLDT 

       250        260        270        280        290        300 
DKVKIFGTKF KVDSTAKLAQ LEKAEREKMK NKIAKISKFG INTFINRQLI YDYPEQLFTD 

       310        320        330        340        350        360 
LGINSIEHAD FEGVERLALV TGGEVVSTFD EPSKCKLGEC DVIEEIMLGE QPFLKFSGCK 

       370        380        390        400        410        420 
AGEACTIVLR GATDQTLDEA ERSLHDALSV LSQTTKETRT VLGGGCAEMV MSKAVDTEAQ 

       430        440        450        460        470        480 
NIDGKKSLAV EAFARALRQL PTILADNAGF DSSELVSKLR SSIYNGISTS GLDLNNGTIA 

       490        500        510        520 
DMRQLGIVES YKLKRAVVSS ASEAAEVLLR VDNIIRARPR TANRQHM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure and function of a second essential member of the heterooligomeric TCP1 chaperonin complex of yeast, TCP1 beta." Miklos D., Caplan S., Mertens D., Hynes G., Pitluk Z., Kashi Y., Harrison-Lavoie K., Stevenson S., Brown C., Barrell B.G., Horwich A.L., Willison K. Proc. Natl. Acad. Sci. U.S.A. 91:2743-2747(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Two yeast genes with similarity to TCP-1 are required for microtubule and actin function in vivo." Chen X., Sullivan D.S., Huffaker T.C. Proc. Natl. Acad. Sci. U.S.A. 91:9111-9115(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	Roemer T., Madden K., Chang J., Snyder M. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 461-527.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51 AND THR-53, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X77675 Genomic DNA. Translation: CAA54745.1.
U16761 Genomic DNA. Translation: AAA53433.1.
Z38059 Genomic DNA. Translation: CAA86136.1.
AY723831 Genomic DNA. Translation: AAU09748.1.
U49845 Genomic DNA. Translation: AAA98665.1.
BK006942 Genomic DNA. Translation: DAA08411.1.

PIR

S48232.

RefSeq

NP_012124.1. NM_001179490.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3P9D	X-ray	3.80	B/J	1-527	[»]
3P9E	X-ray	3.80	b/j	1-527	[»]
4D8Q	X-ray	3.80	B/J	1-527	[»]
4D8R	X-ray	3.80	b/j	1-527	[»]

ProteinModelPortal

P39076.

SMR

P39076. Positions 3-520.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4451N.

IntAct

P39076. 33 interactions.

MINT

MINT-568696.

STRING

4932.YIL142W.

Proteomic databases

PaxDb

P39076.

PeptideAtlas

P39076.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YIL142W; YIL142W; YIL142W.

GeneID

854664.

KEGG

sce:YIL142W.

Organism-specific databases

CYGD

YIL142w.

SGD

S000001404. CCT2.

Phylogenomic databases

eggNOG

COG0459.

GeneTree

ENSGT00550000074930.

HOGENOM

HOG000226736.

K09494.

OMA

NIIRAKP.

OrthoDB

EOG4X6GHQ.

Gene expression databases

Genevestigator

P39076.

GermOnline

YIL142W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR012716. Chap_CCT_beta.
IPR017998. Chaperone_TCP-1.
IPR002194. Chaperonin_TCP-1_CS.
IPR002423. Cpn60/TCP-1.
[Graphical view]

PANTHER

PTHR11353. PTHR11353. 1 hit.
PTHR11353:SF23. PTHR11353:SF23. 1 hit.

Pfam

PF00118. Cpn60_TCP1. 1 hit.
[Graphical view]

PRINTS

PR00304. TCOMPLEXTCP1.

SUPFAM

SSF48592. GroEL-ATPase. 1 hit.

TIGRFAMs

TIGR02341. chap_CCT_beta. 1 hit.

PROSITE

PS00750. TCP1_1. 1 hit.
PS00751. TCP1_2. 1 hit.
PS00995. TCP1_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

977241.

Entry information

Entry name

TCPB_YEAST

Accession

Primary (citable) accession number: P39076
Secondary accession number(s): D6VVE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents