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P39076

- TCPB_YEAST

UniProt

P39076 - TCPB_YEAST

Protein

T-complex protein 1 subunit beta

Gene

CCT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. unfolded protein binding Source: SGD

    GO - Biological processi

    1. protein folding Source: SGD

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31392-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-complex protein 1 subunit beta
    Short name:
    TCP-1-beta
    Alternative name(s):
    CCT-beta
    Gene namesi
    Name:CCT2
    Synonyms:BIN3, TCP2
    Ordered Locus Names:YIL142W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL142w.
    SGDiS000001404. CCT2.

    Subcellular locationi

    GO - Cellular componenti

    1. chaperonin-containing T-complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 527526T-complex protein 1 subunit betaPRO_0000128320Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP39076.
    PaxDbiP39076.
    PeptideAtlasiP39076.

    Expressioni

    Gene expression databases

    GenevestigatoriP39076.

    Interactioni

    Subunit structurei

    Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.

    Protein-protein interaction databases

    BioGridi34850. 76 interactions.
    DIPiDIP-4451N.
    IntActiP39076. 19 interactions.
    MINTiMINT-568696.
    STRINGi4932.YIL142W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3P9DX-ray3.80B/J1-527[»]
    3P9EX-ray3.80b/j1-527[»]
    4AOLX-ray3.80B/b1-527[»]
    4APKX-ray3.80B/b1-527[»]
    4D8QX-ray3.80B/J1-527[»]
    4D8RX-ray3.80b/j1-527[»]
    ProteinModelPortaliP39076.
    SMRiP39076. Positions 3-520.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TCP-1 chaperonin family.Curated

    Phylogenomic databases

    eggNOGiCOG0459.
    GeneTreeiENSGT00550000074930.
    HOGENOMiHOG000226736.
    KOiK09494.
    OMAiQDRDHFA.
    OrthoDBiEOG773XR5.

    Family and domain databases

    Gene3Di1.10.560.10. 2 hits.
    3.30.260.10. 2 hits.
    3.50.7.10. 1 hit.
    InterProiIPR012716. Chap_CCT_beta.
    IPR017998. Chaperone_TCP-1.
    IPR002194. Chaperonin_TCP-1_CS.
    IPR002423. Cpn60/TCP-1.
    IPR027409. GroEL-like_apical_dom.
    IPR027413. GROEL-like_equatorial.
    IPR027410. TCP-1-like_intermed.
    [Graphical view]
    PANTHERiPTHR11353. PTHR11353. 1 hit.
    PfamiPF00118. Cpn60_TCP1. 1 hit.
    [Graphical view]
    PRINTSiPR00304. TCOMPLEXTCP1.
    SUPFAMiSSF48592. SSF48592. 2 hits.
    SSF52029. SSF52029. 1 hit.
    TIGRFAMsiTIGR02341. chap_CCT_beta. 1 hit.
    PROSITEiPS00750. TCP1_1. 1 hit.
    PS00751. TCP1_2. 1 hit.
    PS00995. TCP1_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39076-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVQIFGDQV TEERAENARL SAFVGAIAVG DLVKSTLGPK GMDKLLQSAS    50
    SNTCMVTNDG ATILKSIPLD NPAAKVLVNI SKVQDDEVGD GTTSVTVLSA 100
    ELLREAEKLI DQSKIHPQTI IEGYRLASAA ALDALTKAAV DNSHDKTMFR 150
    EDLIHIAKTT LSSKILSQDK DHFAELATNA ILRLKGSTNL EHIQIIKILG 200
    GKLSDSFLDE GFILAKKFGN NQPKRIENAK ILIANTTLDT DKVKIFGTKF 250
    KVDSTAKLAQ LEKAEREKMK NKIAKISKFG INTFINRQLI YDYPEQLFTD 300
    LGINSIEHAD FEGVERLALV TGGEVVSTFD EPSKCKLGEC DVIEEIMLGE 350
    QPFLKFSGCK AGEACTIVLR GATDQTLDEA ERSLHDALSV LSQTTKETRT 400
    VLGGGCAEMV MSKAVDTEAQ NIDGKKSLAV EAFARALRQL PTILADNAGF 450
    DSSELVSKLR SSIYNGISTS GLDLNNGTIA DMRQLGIVES YKLKRAVVSS 500
    ASEAAEVLLR VDNIIRARPR TANRQHM 527
    Length:527
    Mass (Da):57,203
    Last modified:February 1, 1995 - v1
    Checksum:iC5DB2BC3660EC7E6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77675 Genomic DNA. Translation: CAA54745.1.
    U16761 Genomic DNA. Translation: AAA53433.1.
    Z38059 Genomic DNA. Translation: CAA86136.1.
    AY723831 Genomic DNA. Translation: AAU09748.1.
    U49845 Genomic DNA. Translation: AAA98665.1.
    BK006942 Genomic DNA. Translation: DAA08411.1.
    PIRiS48232.
    RefSeqiNP_012124.1. NM_001179490.1.

    Genome annotation databases

    EnsemblFungiiYIL142W; YIL142W; YIL142W.
    GeneIDi854664.
    KEGGisce:YIL142W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77675 Genomic DNA. Translation: CAA54745.1 .
    U16761 Genomic DNA. Translation: AAA53433.1 .
    Z38059 Genomic DNA. Translation: CAA86136.1 .
    AY723831 Genomic DNA. Translation: AAU09748.1 .
    U49845 Genomic DNA. Translation: AAA98665.1 .
    BK006942 Genomic DNA. Translation: DAA08411.1 .
    PIRi S48232.
    RefSeqi NP_012124.1. NM_001179490.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3P9D X-ray 3.80 B/J 1-527 [» ]
    3P9E X-ray 3.80 b/j 1-527 [» ]
    4AOL X-ray 3.80 B/b 1-527 [» ]
    4APK X-ray 3.80 B/b 1-527 [» ]
    4D8Q X-ray 3.80 B/J 1-527 [» ]
    4D8R X-ray 3.80 b/j 1-527 [» ]
    ProteinModelPortali P39076.
    SMRi P39076. Positions 3-520.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34850. 76 interactions.
    DIPi DIP-4451N.
    IntActi P39076. 19 interactions.
    MINTi MINT-568696.
    STRINGi 4932.YIL142W.

    Proteomic databases

    MaxQBi P39076.
    PaxDbi P39076.
    PeptideAtlasi P39076.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL142W ; YIL142W ; YIL142W .
    GeneIDi 854664.
    KEGGi sce:YIL142W.

    Organism-specific databases

    CYGDi YIL142w.
    SGDi S000001404. CCT2.

    Phylogenomic databases

    eggNOGi COG0459.
    GeneTreei ENSGT00550000074930.
    HOGENOMi HOG000226736.
    KOi K09494.
    OMAi QDRDHFA.
    OrthoDBi EOG773XR5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31392-MONOMER.

    Miscellaneous databases

    NextBioi 977241.
    PROi P39076.

    Gene expression databases

    Genevestigatori P39076.

    Family and domain databases

    Gene3Di 1.10.560.10. 2 hits.
    3.30.260.10. 2 hits.
    3.50.7.10. 1 hit.
    InterProi IPR012716. Chap_CCT_beta.
    IPR017998. Chaperone_TCP-1.
    IPR002194. Chaperonin_TCP-1_CS.
    IPR002423. Cpn60/TCP-1.
    IPR027409. GroEL-like_apical_dom.
    IPR027413. GROEL-like_equatorial.
    IPR027410. TCP-1-like_intermed.
    [Graphical view ]
    PANTHERi PTHR11353. PTHR11353. 1 hit.
    Pfami PF00118. Cpn60_TCP1. 1 hit.
    [Graphical view ]
    PRINTSi PR00304. TCOMPLEXTCP1.
    SUPFAMi SSF48592. SSF48592. 2 hits.
    SSF52029. SSF52029. 1 hit.
    TIGRFAMsi TIGR02341. chap_CCT_beta. 1 hit.
    PROSITEi PS00750. TCP1_1. 1 hit.
    PS00751. TCP1_2. 1 hit.
    PS00995. TCP1_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and function of a second essential member of the heterooligomeric TCP1 chaperonin complex of yeast, TCP1 beta."
      Miklos D., Caplan S., Mertens D., Hynes G., Pitluk Z., Kashi Y., Harrison-Lavoie K., Stevenson S., Brown C., Barrell B.G., Horwich A.L., Willison K.
      Proc. Natl. Acad. Sci. U.S.A. 91:2743-2747(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. "Two yeast genes with similarity to TCP-1 are required for microtubule and actin function in vivo."
      Chen X., Sullivan D.S., Huffaker T.C.
      Proc. Natl. Acad. Sci. U.S.A. 91:9111-9115(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Roemer T., Madden K., Chang J., Snyder M.
      Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 461-527.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTCPB_YEAST
    AccessioniPrimary (citable) accession number: P39076
    Secondary accession number(s): D6VVE5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3