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P39076 (TCPB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-complex protein 1 subunit beta

Short name=TCP-1-beta
Alternative name(s):
CCT-beta
Gene names
Name:CCT2
Synonyms:BIN3, TCP2
Ordered Locus Names:YIL142W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.

Subunit structure

Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the TCP-1 chaperonin family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionChaperone
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from direct assay PubMed 16762366. Source: SGD

   Cellular_componentchaperonin-containing T-complex

Inferred from direct assay PubMed 16762366. Source: SGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

unfolded protein binding

Inferred from direct assay PubMed 16762366. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 527526T-complex protein 1 subunit beta
PRO_0000128320

Amino acid modifications

Modified residue21N-acetylserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P39076 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: C5DB2BC3660EC7E6

FASTA52757,203
        10         20         30         40         50         60 
MSVQIFGDQV TEERAENARL SAFVGAIAVG DLVKSTLGPK GMDKLLQSAS SNTCMVTNDG 

        70         80         90        100        110        120 
ATILKSIPLD NPAAKVLVNI SKVQDDEVGD GTTSVTVLSA ELLREAEKLI DQSKIHPQTI 

       130        140        150        160        170        180 
IEGYRLASAA ALDALTKAAV DNSHDKTMFR EDLIHIAKTT LSSKILSQDK DHFAELATNA 

       190        200        210        220        230        240 
ILRLKGSTNL EHIQIIKILG GKLSDSFLDE GFILAKKFGN NQPKRIENAK ILIANTTLDT 

       250        260        270        280        290        300 
DKVKIFGTKF KVDSTAKLAQ LEKAEREKMK NKIAKISKFG INTFINRQLI YDYPEQLFTD 

       310        320        330        340        350        360 
LGINSIEHAD FEGVERLALV TGGEVVSTFD EPSKCKLGEC DVIEEIMLGE QPFLKFSGCK 

       370        380        390        400        410        420 
AGEACTIVLR GATDQTLDEA ERSLHDALSV LSQTTKETRT VLGGGCAEMV MSKAVDTEAQ 

       430        440        450        460        470        480 
NIDGKKSLAV EAFARALRQL PTILADNAGF DSSELVSKLR SSIYNGISTS GLDLNNGTIA 

       490        500        510        520 
DMRQLGIVES YKLKRAVVSS ASEAAEVLLR VDNIIRARPR TANRQHM 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure and function of a second essential member of the heterooligomeric TCP1 chaperonin complex of yeast, TCP1 beta."
Miklos D., Caplan S., Mertens D., Hynes G., Pitluk Z., Kashi Y., Harrison-Lavoie K., Stevenson S., Brown C., Barrell B.G., Horwich A.L., Willison K.
Proc. Natl. Acad. Sci. U.S.A. 91:2743-2747(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Two yeast genes with similarity to TCP-1 are required for microtubule and actin function in vivo."
Chen X., Sullivan D.S., Huffaker T.C.
Proc. Natl. Acad. Sci. U.S.A. 91:9111-9115(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]Roemer T., Madden K., Chang J., Snyder M.
Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 461-527.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77675 Genomic DNA. Translation: CAA54745.1.
U16761 Genomic DNA. Translation: AAA53433.1.
Z38059 Genomic DNA. Translation: CAA86136.1.
AY723831 Genomic DNA. Translation: AAU09748.1.
U49845 Genomic DNA. Translation: AAA98665.1.
BK006942 Genomic DNA. Translation: DAA08411.1.
PIRS48232.
RefSeqNP_012124.1. NM_001179490.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3P9DX-ray3.80B/J1-527[»]
3P9EX-ray3.80b/j1-527[»]
4AOLX-ray3.80B/b1-527[»]
4APKX-ray3.80B/b1-527[»]
4D8QX-ray3.80B/J1-527[»]
4D8RX-ray3.80b/j1-527[»]
ProteinModelPortalP39076.
SMRP39076. Positions 3-520.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34850. 76 interactions.
DIPDIP-4451N.
IntActP39076. 19 interactions.
MINTMINT-568696.
STRING4932.YIL142W.

Proteomic databases

PaxDbP39076.
PeptideAtlasP39076.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL142W; YIL142W; YIL142W.
GeneID854664.
KEGGsce:YIL142W.

Organism-specific databases

CYGDYIL142w.
SGDS000001404. CCT2.

Phylogenomic databases

eggNOGCOG0459.
GeneTreeENSGT00550000074930.
HOGENOMHOG000226736.
KOK09494.
OMACAEMVMS.
OrthoDBEOG773XR5.

Enzyme and pathway databases

BioCycYEAST:G3O-31392-MONOMER.

Gene expression databases

GenevestigatorP39076.

Family and domain databases

Gene3D1.10.560.10. 2 hits.
3.30.260.10. 2 hits.
3.50.7.10. 1 hit.
InterProIPR012716. Chap_CCT_beta.
IPR017998. Chaperone_TCP-1.
IPR002194. Chaperonin_TCP-1_CS.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
IPR027410. TCP-1-like_intermed.
[Graphical view]
PANTHERPTHR11353. PTHR11353. 1 hit.
PfamPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSPR00304. TCOMPLEXTCP1.
SUPFAMSSF48592. SSF48592. 2 hits.
SSF52029. SSF52029. 1 hit.
TIGRFAMsTIGR02341. chap_CCT_beta. 1 hit.
PROSITEPS00750. TCP1_1. 1 hit.
PS00751. TCP1_2. 1 hit.
PS00995. TCP1_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977241.
PROP39076.

Entry information

Entry nameTCPB_YEAST
AccessionPrimary (citable) accession number: P39076
Secondary accession number(s): D6VVE5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 19, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references