ID   BMRR_BACSU              Reviewed;         278 AA.
AC   P39075;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   24-OCT-2003, sequence version 3.
DT   27-MAR-2024, entry version 157.
DE   RecName: Full=Multidrug-efflux transporter 1 regulator;
GN   Name=bmrR; Synonyms=bmr1R; OrderedLocusNames=BSU24020;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 /
RC   NCIMB 3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=7961792; DOI=10.1016/s0021-9258(18)46956-6;
RA   Ahmed M., Borsch C.M., Taylor S.S., Vazquez-Laslop N., Neyfakh A.A.;
RT   "A protein that activates expression of a multidrug efflux transporter upon
RT   binding the transporter substrates.";
RL   J. Biol. Chem. 269:28506-28513(1994).
RN   [2]
RP   SEQUENCE REVISION.
RA   Neyfakh A.A.;
RL   Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [5]
RP   SEQUENCE REVISION.
RX   PubMed=10568751; DOI=10.1101/gr.9.11.1116;
RA   Medigue C., Rose M., Viari A., Danchin A.;
RT   "Detecting and analyzing DNA sequencing errors: toward a higher quality of
RT   the Bacillus subtilis genome sequence.";
RL   Genome Res. 9:1116-1127(1999).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 120-228.
RX   PubMed=10025401; DOI=10.1016/s0092-8674(00)80548-6;
RA   Zheleznova E.E., Markham P.N., Neyfakh A.A., Brennan R.G.;
RT   "Structural basis of multidrug recognition by BmrR, a transcription
RT   activator of a multidrug transporter.";
RL   Cell 96:353-362(1999).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (3.12 ANGSTROMS).
RX   PubMed=11201751; DOI=10.1038/35053138;
RA   Heldwein E.E., Brennan R.G.;
RT   "Crystal structure of the transcription activator BmrR bound to DNA and a
RT   drug.";
RL   Nature 409:378-382(2001).
CC   -!- FUNCTION: Activates transcription of the bmr gene in response to
CC       structurally dissimilar drugs. Binds rhodamine as an inducer.
CC   -!- SUBUNIT: Binds DNA as a homodimer.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA23495.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; L25604; AAB81540.2; -; Genomic_DNA.
DR   EMBL; D84432; BAA23495.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL009126; CAB14333.2; -; Genomic_DNA.
DR   PIR; E69595; E69595.
DR   RefSeq; NP_390282.2; NC_000964.3.
DR   RefSeq; WP_003230325.1; NZ_JNCM01000036.1.
DR   PDB; 1BOW; X-ray; 2.70 A; A=121-278.
DR   PDB; 1EXI; X-ray; 3.12 A; A=1-278.
DR   PDB; 1EXJ; X-ray; 3.00 A; A=1-278.
DR   PDB; 1R8E; X-ray; 2.40 A; A=1-278.
DR   PDB; 2BOW; X-ray; 2.80 A; A=121-278.
DR   PDB; 3D6Y; X-ray; 2.70 A; A=1-278.
DR   PDB; 3D6Z; X-ray; 2.60 A; A=1-278.
DR   PDB; 3D70; X-ray; 2.80 A; A=2-278.
DR   PDB; 3D71; X-ray; 2.80 A; A=1-276.
DR   PDB; 3IAO; X-ray; 2.80 A; A=1-278.
DR   PDB; 3Q1M; X-ray; 3.20 A; A=1-276.
DR   PDB; 3Q2Y; X-ray; 2.95 A; A=1-276.
DR   PDB; 3Q3D; X-ray; 2.79 A; A=1-276.
DR   PDB; 3Q5P; X-ray; 2.94 A; A=1-276.
DR   PDB; 3Q5R; X-ray; 3.05 A; A=1-276.
DR   PDB; 3Q5S; X-ray; 3.10 A; A=1-276.
DR   PDB; 7CKQ; EM; 4.40 A; G/I=1-278.
DR   PDBsum; 1BOW; -.
DR   PDBsum; 1EXI; -.
DR   PDBsum; 1EXJ; -.
DR   PDBsum; 1R8E; -.
DR   PDBsum; 2BOW; -.
DR   PDBsum; 3D6Y; -.
DR   PDBsum; 3D6Z; -.
DR   PDBsum; 3D70; -.
DR   PDBsum; 3D71; -.
DR   PDBsum; 3IAO; -.
DR   PDBsum; 3Q1M; -.
DR   PDBsum; 3Q2Y; -.
DR   PDBsum; 3Q3D; -.
DR   PDBsum; 3Q5P; -.
DR   PDBsum; 3Q5R; -.
DR   PDBsum; 3Q5S; -.
DR   PDBsum; 7CKQ; -.
DR   AlphaFoldDB; P39075; -.
DR   EMDB; EMD-30390; -.
DR   SMR; P39075; -.
DR   DIP; DIP-59692N; -.
DR   STRING; 224308.BSU24020; -.
DR   PaxDb; 224308-BSU24020; -.
DR   EnsemblBacteria; CAB14333; CAB14333; BSU_24020.
DR   GeneID; 938676; -.
DR   KEGG; bsu:BSU24020; -.
DR   PATRIC; fig|224308.179.peg.2616; -.
DR   eggNOG; COG0789; Bacteria.
DR   eggNOG; COG4978; Bacteria.
DR   InParanoid; P39075; -.
DR   OrthoDB; 9773308at2; -.
DR   PhylomeDB; P39075; -.
DR   BioCyc; BSUB:BSU24020-MONOMER; -.
DR   EvolutionaryTrace; P39075; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   CDD; cd01107; HTH_BmrR; 1.
DR   Gene3D; 1.10.1660.10; -; 1.
DR   Gene3D; 3.20.80.10; Regulatory factor, effector binding domain; 1.
DR   Gene3D; 1.20.5.490; Single helix bin; 1.
DR   InterPro; IPR009061; DNA-bd_dom_put_sf.
DR   InterPro; IPR029442; GyrI-like.
DR   InterPro; IPR000551; MerR-type_HTH_dom.
DR   InterPro; IPR047057; MerR_fam.
DR   InterPro; IPR011256; Reg_factor_effector_dom_sf.
DR   PANTHER; PTHR30204:SF87; MULTIDRUG-EFFLUX TRANSPORTER 1 REGULATOR; 1.
DR   PANTHER; PTHR30204; REDOX-CYCLING DRUG-SENSING TRANSCRIPTIONAL ACTIVATOR SOXR; 1.
DR   Pfam; PF06445; GyrI-like; 1.
DR   Pfam; PF13411; MerR_1; 1.
DR   SMART; SM00422; HTH_MERR; 1.
DR   SUPFAM; SSF55136; Probable bacterial effector-binding domain; 1.
DR   SUPFAM; SSF46955; Putative DNA-binding domain; 1.
DR   PROSITE; PS00552; HTH_MERR_1; 1.
DR   PROSITE; PS50937; HTH_MERR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..278
FT                   /note="Multidrug-efflux transporter 1 regulator"
FT                   /id="PRO_0000098109"
FT   DOMAIN          5..75
FT                   /note="HTH merR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT   DNA_BIND        8..27
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT   STRAND          5..7
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   HELIX           8..15
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   HELIX           19..27
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          34..36
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   TURN            38..40
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          43..46
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   HELIX           48..51
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   HELIX           52..61
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   HELIX           66..72
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   HELIX           77..116
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          119..121
FT                   /evidence="ECO:0007829|PDB:1EXJ"
FT   STRAND          125..129
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          132..138
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   TURN            144..146
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   HELIX           149..152
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   HELIX           153..163
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          170..174
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   HELIX           182..184
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          188..193
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          207..212
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          214..223
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   HELIX           226..242
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          247..258
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          262..264
FT                   /evidence="ECO:0007829|PDB:1R8E"
FT   STRAND          268..276
FT                   /evidence="ECO:0007829|PDB:1R8E"
SQ   SEQUENCE   278 AA;  32582 MW;  184AEF0274991E73 CRC64;
     MKESYYSIGE VSKLANVSIK ALRYYDKIDL FKPAYVDPDT SYRYYTDSQL IHLDLIKSLK
     YIGTPLEEMK KAQDLEMEEL FAFYTEQERQ IREKLDFLSA LEQTISLVKK RMKRQMEYPA
     LGEVFVLDEE EIRIIQTEAE GIGPENVLNA SYSKLKKFIE SADGFTNNSY GATFSFQPYT
     SIDEMTYRHI FTPVLTNKQI SSITPDMEIT TIPKGRYACI AYNFSPEHYF LNLQKLIKYI
     ADRQLTVVSD VYELIIPIHY SPKKQEEYRV EMKIRIAE
//