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Protein

Meiotic mRNA stability protein kinase SSN3

Gene

SSN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions. Phosphorylates GCN4, promoting its ubiquitin-mediated degradation, and MSN2, promoting its nuclear exclusion. Phosphorylates STE12, thereby promoting its degradation and inhibition of filamentous growth. Phosphorylates GAL4, and this phosphorylation is required for efficient galactose-inducible transcription. Also phosphorylates BDF1 and the TAF2 subunit of the TFIID complex.17 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.7 Publications
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.7 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei183 – 1831ATPCurated
Active sitei286 – 2861Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 899ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II carboxy-terminal domain kinase activity Source: SGD

GO - Biological processi

  • meiotic cell cycle Source: UniProtKB-KW
  • negative regulation of filamentous growth Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD
  • protein destabilization Source: SGD
  • protein phosphorylation Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Meiosis, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33956-MONOMER.
BRENDAi2.7.11.22. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic mRNA stability protein kinase SSN3 (EC:2.7.11.227 Publications, EC:2.7.11.237 Publications)
Alternative name(s):
Cyclin-dependent kinase 8
Suppressor of RNA polymerase B SRB10
Gene namesi
Name:SSN3
Synonyms:ARE1, CDK8, GIG2, SRB10, UME5
Ordered Locus Names:YPL042C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL042C.
SGDiS000005963. SSN3.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi183 – 1831K → R in UME5-4; loss of activity. 1 Publication
Mutagenesisi304 – 3041D → A: Abrogates kinase activity and transcriptional repression. 7 Publications

Chemistry

ChEMBLiCHEMBL5395.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Meiotic mRNA stability protein kinase SSN3PRO_0000086784Add
BLAST

Proteomic databases

MaxQBiP39073.

PTM databases

iPTMnetiP39073.

Expressioni

Inductioni

Protein level and kinase activity are reduced during nitrogen starvation.2 Publications

Interactioni

Subunit structurei

Component of the SRB8-11 complex which consists of SRB8, SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair also associate with the RNA polymerase II holoenzyme. Interacts with TUP1.6 Publications

Protein-protein interaction databases

BioGridi36137. 263 interactions.
DIPiDIP-2574N.
IntActiP39073. 24 interactions.
MINTiMINT-420230.

Chemistry

BindingDBiP39073.

Structurei

3D structure databases

ProteinModelPortaliP39073.
SMRiP39073. Positions 70-107, 150-486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini75 – 463389Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000064012.
HOGENOMiHOG000233024.
InParanoidiP39073.
KOiK02208.
OMAiREIQHEN.
OrthoDBiEOG092C2FL8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNGKDRAQN SYQPMYQRPM QVQGQQQAQS FVGKKNTIGS VHGKAPMLMA
60 70 80 90 100
NNDVFTIGPY RARKDRMRVS VLEKYEVIGY IAAGTYGKVY KAKRQINSGT
110 120 130 140 150
NSANGSSLNG TNAKIPQFDS TQPKSSSSMD MQANTNALRR NLLKDEGVTP
160 170 180 190 200
GRIRTTREDV SPHYNSQKQT LIKKPLTVFY AIKKFKTEKD GVEQLHYTGI
210 220 230 240 250
SQSACREMAL CRELHNKHLT TLVEIFLERK CVHMVYEYAE HDLLQIIHFH
260 270 280 290 300
SHPEKRMIPP RMVRSIMWQL LDGVSYLHQN WVLHRDLKPA NIMVTIDGCV
310 320 330 340 350
KIGDLGLARK FHNMLQTLYT GDKVVVTIWY RAPELLLGAR HYTPAVDLWS
360 370 380 390 400
VGCIFAELIG LQPIFKGEEA KLDSKKTVPF QVNQLQRILE VLGTPDQKIW
410 420 430 440 450
PYLEKYPEYD QITKFPKYRD NLATWYHSAG GRDKHALSLL YHLLNYDPIK
460 470 480 490 500
RIDAFNALEH KYFTESDIPV SENVFEGLTY KYPARRIHTN DNDIMNLGSR
510 520 530 540 550
TKNNTQASGI TAGAAANALG GLGVNRRILA AAAAAAAAVS GNNASDEPSR

KKNRR
Length:555
Mass (Da):62,847
Last modified:September 5, 2006 - v3
Checksum:iC7B6DDC868CCD61E
GO

Sequence cautioni

The sequence AAC13785 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27151 Genomic DNA. Translation: AAA35193.1.
U20222 Genomic DNA. Translation: AAC13785.1. Different initiation.
U44030 Genomic DNA. Translation: AAB68178.1.
BK006949 Genomic DNA. Translation: DAA11387.1.
PIRiS50157.
RefSeqiNP_015283.1. NM_001183856.1.

Genome annotation databases

EnsemblFungiiYPL042C; YPL042C; YPL042C.
GeneIDi856065.
KEGGisce:YPL042C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27151 Genomic DNA. Translation: AAA35193.1.
U20222 Genomic DNA. Translation: AAC13785.1. Different initiation.
U44030 Genomic DNA. Translation: AAB68178.1.
BK006949 Genomic DNA. Translation: DAA11387.1.
PIRiS50157.
RefSeqiNP_015283.1. NM_001183856.1.

3D structure databases

ProteinModelPortaliP39073.
SMRiP39073. Positions 70-107, 150-486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36137. 263 interactions.
DIPiDIP-2574N.
IntActiP39073. 24 interactions.
MINTiMINT-420230.

Chemistry

BindingDBiP39073.
ChEMBLiCHEMBL5395.

PTM databases

iPTMnetiP39073.

Proteomic databases

MaxQBiP39073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL042C; YPL042C; YPL042C.
GeneIDi856065.
KEGGisce:YPL042C.

Organism-specific databases

EuPathDBiFungiDB:YPL042C.
SGDiS000005963. SSN3.

Phylogenomic databases

GeneTreeiENSGT00530000064012.
HOGENOMiHOG000233024.
InParanoidiP39073.
KOiK02208.
OMAiREIQHEN.
OrthoDBiEOG092C2FL8.

Enzyme and pathway databases

BioCyciYEAST:G3O-33956-MONOMER.
BRENDAi2.7.11.22. 984.

Miscellaneous databases

PROiP39073.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSN3_YEAST
AccessioniPrimary (citable) accession number: P39073
Secondary accession number(s): D6W3X1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: September 5, 2006
Last modified: September 7, 2016
This is version 162 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.