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P39073

- SSN3_YEAST

UniProt

P39073 - SSN3_YEAST

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Protein

Meiotic mRNA stability protein kinase SSN3

Gene

SSN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions. Phosphorylates GCN4, promoting its ubiquitin-mediated degradation, and MSN2, promoting its nuclear exclusion. Phosphorylates STE12, thereby promoting its degradation and inhibition of filamentous growth. Phosphorylates GAL4, and this phosphorylation is required for efficient galactose-inducible transcription. Also phosphorylates BDF1 and the TAF2 subunit of the TFIID complex.17 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei183 – 1831ATPCurated
Active sitei286 – 2861Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 899ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: SGD
  3. metal ion binding Source: UniProtKB-KW
  4. RNA polymerase II carboxy-terminal domain kinase activity Source: SGD

GO - Biological processi

  1. meiotic nuclear division Source: UniProtKB-KW
  2. negative regulation of filamentous growth Source: SGD
  3. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  4. nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
  5. phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  6. positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD
  7. protein destabilization Source: SGD
  8. protein phosphorylation Source: SGD
  9. regulation of cell cycle Source: GOC
  10. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Meiosis, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33956-MONOMER.
BRENDAi2.7.11.22. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic mRNA stability protein kinase SSN3 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cyclin-dependent kinase 8
Suppressor of RNA polymerase B SRB10
Gene namesi
Name:SSN3
Synonyms:ARE1, CDK8, GIG2, SRB10, UME5
Ordered Locus Names:YPL042C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL042c.
SGDiS000005963. SSN3.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. mediator complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi183 – 1831K → R in UME5-4; loss of activity. 1 Publication
Mutagenesisi304 – 3041D → A: Abrogates kinase activity and transcriptional repression. 7 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Meiotic mRNA stability protein kinase SSN3PRO_0000086784Add
BLAST

Proteomic databases

MaxQBiP39073.
PaxDbiP39073.

Expressioni

Inductioni

Protein level and kinase activity are reduced during nitrogen starvation.2 Publications

Gene expression databases

GenevestigatoriP39073.

Interactioni

Subunit structurei

Component of the SRB8-11 complex which consists of SRB8, SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair also associate with the RNA polymerase II holoenzyme. Interacts with TUP1.6 Publications

Protein-protein interaction databases

BioGridi36137. 266 interactions.
DIPiDIP-2574N.
IntActiP39073. 24 interactions.
MINTiMINT-420230.
STRINGi4932.YPL042C.

Structurei

3D structure databases

ProteinModelPortaliP39073.
SMRiP39073. Positions 150-486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini75 – 463389Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000064012.
HOGENOMiHOG000233024.
InParanoidiP39073.
KOiK02208.
OMAiFHSHPEK.
OrthoDBiEOG7K3TWD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39073-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNGKDRAQN SYQPMYQRPM QVQGQQQAQS FVGKKNTIGS VHGKAPMLMA
60 70 80 90 100
NNDVFTIGPY RARKDRMRVS VLEKYEVIGY IAAGTYGKVY KAKRQINSGT
110 120 130 140 150
NSANGSSLNG TNAKIPQFDS TQPKSSSSMD MQANTNALRR NLLKDEGVTP
160 170 180 190 200
GRIRTTREDV SPHYNSQKQT LIKKPLTVFY AIKKFKTEKD GVEQLHYTGI
210 220 230 240 250
SQSACREMAL CRELHNKHLT TLVEIFLERK CVHMVYEYAE HDLLQIIHFH
260 270 280 290 300
SHPEKRMIPP RMVRSIMWQL LDGVSYLHQN WVLHRDLKPA NIMVTIDGCV
310 320 330 340 350
KIGDLGLARK FHNMLQTLYT GDKVVVTIWY RAPELLLGAR HYTPAVDLWS
360 370 380 390 400
VGCIFAELIG LQPIFKGEEA KLDSKKTVPF QVNQLQRILE VLGTPDQKIW
410 420 430 440 450
PYLEKYPEYD QITKFPKYRD NLATWYHSAG GRDKHALSLL YHLLNYDPIK
460 470 480 490 500
RIDAFNALEH KYFTESDIPV SENVFEGLTY KYPARRIHTN DNDIMNLGSR
510 520 530 540 550
TKNNTQASGI TAGAAANALG GLGVNRRILA AAAAAAAAVS GNNASDEPSR

KKNRR
Length:555
Mass (Da):62,847
Last modified:September 5, 2006 - v3
Checksum:iC7B6DDC868CCD61E
GO

Sequence cautioni

The sequence AAC13785.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27151 Genomic DNA. Translation: AAA35193.1.
U20222 Genomic DNA. Translation: AAC13785.1. Different initiation.
U44030 Genomic DNA. Translation: AAB68178.1.
BK006949 Genomic DNA. Translation: DAA11387.1.
PIRiS50157.
RefSeqiNP_015283.1. NM_001183856.1.

Genome annotation databases

EnsemblFungiiYPL042C; YPL042C; YPL042C.
GeneIDi856065.
KEGGisce:YPL042C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27151 Genomic DNA. Translation: AAA35193.1 .
U20222 Genomic DNA. Translation: AAC13785.1 . Different initiation.
U44030 Genomic DNA. Translation: AAB68178.1 .
BK006949 Genomic DNA. Translation: DAA11387.1 .
PIRi S50157.
RefSeqi NP_015283.1. NM_001183856.1.

3D structure databases

ProteinModelPortali P39073.
SMRi P39073. Positions 150-486.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36137. 266 interactions.
DIPi DIP-2574N.
IntActi P39073. 24 interactions.
MINTi MINT-420230.
STRINGi 4932.YPL042C.

Chemistry

BindingDBi P39073.
ChEMBLi CHEMBL5395.

Proteomic databases

MaxQBi P39073.
PaxDbi P39073.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL042C ; YPL042C ; YPL042C .
GeneIDi 856065.
KEGGi sce:YPL042C.

Organism-specific databases

CYGDi YPL042c.
SGDi S000005963. SSN3.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000064012.
HOGENOMi HOG000233024.
InParanoidi P39073.
KOi K02208.
OMAi FHSHPEK.
OrthoDBi EOG7K3TWD.

Enzyme and pathway databases

BioCyci YEAST:G3O-33956-MONOMER.
BRENDAi 2.7.11.22. 984.

Miscellaneous databases

NextBioi 981047.
PROi P39073.

Gene expression databases

Genevestigatori P39073.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 2 hits.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast UME5 gene regulates the stability of meiotic mRNAs in response to glucose."
    Surosky R.T., Strich R., Esposito R.E.
    Mol. Cell. Biol. 14:3446-3458(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF LYS-183.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH SSN8.
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: FUNCTION, INDUCTION.
  6. "Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases."
    Hengartner C.J., Myer V.E., Liao S.-M., Wilson C.J., Koh S.S., Young R.A.
    Mol. Cell 2:43-53(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH SSN8 AND THE RNA POLYMERASE II HOLOENZYME, MUTAGENESIS OF ASP-304.
  7. "GAL4 is regulated by the RNA polymerase II holoenzyme-associated cyclin-dependent protein kinase SRB10/CDK8."
    Hirst M., Kobor M.S., Kuriakose N., Greenblatt J., Sadowski I.
    Mol. Cell 3:673-678(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-304.
  8. "Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase."
    Chi Y., Huddleston M.J., Zhang X., Young R.A., Annan R.S., Carr S.A., Deshaies R.J.
    Genes Dev. 15:1078-1092(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-304.
  9. "The structural and functional organization of the yeast mediator complex."
    Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
    J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED1 AND MED4, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  10. "Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression."
    Zaman Z., Ansari A.Z., Koh S.S., Young R., Ptashne M.
    Proc. Natl. Acad. Sci. U.S.A. 98:2550-2554(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SSN8 AND TUP1, MUTAGENESIS OF ASP-304.
  11. "A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast."
    Borggrefe T., Davis R., Erdjument-Bromage H., Tempst P., Kornberg R.D.
    J. Biol. Chem. 277:44202-44207(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SRB8-11 COMPLEX, FUNCTION OF THE SRB8-11 COMPLEX, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-304.
  12. "Srb10/Cdk8 regulates yeast filamentous growth by phosphorylating the transcription factor Ste12."
    Nelson C., Goto S., Lund K., Hung W., Sadowski I.
    Nature 421:187-190(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, INDUCTION, MUTAGENESIS OF ASP-304.
  13. Cited for: NOMENCLATURE.
  14. "Two cyclin-dependent kinases promote RNA polymerase II transcription and formation of the scaffold complex."
    Liu Y., Kung C., Fishburn J., Ansari A.Z., Shokat K.M., Hahn S.
    Mol. Cell. Biol. 24:1721-1735(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH RNA POLYMERASE II.
  15. "Promoter-dependent roles for the Srb10 cyclin-dependent kinase and the Hda1 deacetylase in Tup1-mediated repression in Saccharomyces cerevisiae."
    Green S.R., Johnson A.D.
    Mol. Biol. Cell 15:4191-4202(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-304.
  16. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
  17. "Site-specific Srb10-dependent phosphorylation of the yeast Mediator subunit Med2 regulates gene expression from the 2-micrometer plasmid."
    Hallberg M., Polozkov G.V., Hu G.-Z., Beve J., Gustafsson C.M., Ronne H., Bjoerklund S.
    Proc. Natl. Acad. Sci. U.S.A. 101:3370-3375(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
    Nair D., Kim Y., Myers L.C.
    J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
  19. "Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
    van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
    Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  20. "The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription."
    Larschan E., Winston F.
    Mol. Cell. Biol. 25:114-123(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE SRB8-11 COMPLEX.
  21. "Transcriptional activating regions target attached substrates to a cyclin-dependent kinase."
    Ansari A.Z., Ogirala A., Ptashne M.
    Proc. Natl. Acad. Sci. U.S.A. 102:2346-2349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  22. "Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA."
    Andrau J.-C., van de Pasch L., Lijnzaad P., Bijma T., Koerkamp M.G., van de Peppel J., Werner M., Holstege F.C.P.
    Mol. Cell 22:179-192(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSSN3_YEAST
AccessioniPrimary (citable) accession number: P39073
Secondary accession number(s): D6W3X1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: September 5, 2006
Last modified: November 26, 2014
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3