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P39073

- SSN3_YEAST

UniProt

P39073 - SSN3_YEAST

Protein

Meiotic mRNA stability protein kinase SSN3

Gene

SSN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 3 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions. Phosphorylates GCN4, promoting its ubiquitin-mediated degradation, and MSN2, promoting its nuclear exclusion. Phosphorylates STE12, thereby promoting its degradation and inhibition of filamentous growth. Phosphorylates GAL4, and this phosphorylation is required for efficient galactose-inducible transcription. Also phosphorylates BDF1 and the TAF2 subunit of the TFIID complex.17 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei183 – 1831ATPCurated
    Active sitei286 – 2861Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi81 – 899ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: SGD
    3. metal ion binding Source: UniProtKB-KW
    4. RNA polymerase II carboxy-terminal domain kinase activity Source: SGD

    GO - Biological processi

    1. meiotic nuclear division Source: UniProtKB-KW
    2. negative regulation of filamentous growth Source: SGD
    3. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    4. nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
    5. phosphorylation of RNA polymerase II C-terminal domain Source: SGD
    6. positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD
    7. protein destabilization Source: SGD
    8. protein phosphorylation Source: SGD
    9. regulation of cell cycle Source: GOC
    10. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Meiosis, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33956-MONOMER.
    BRENDAi2.7.11.22. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Meiotic mRNA stability protein kinase SSN3 (EC:2.7.11.22, EC:2.7.11.23)
    Alternative name(s):
    Cyclin-dependent kinase 8
    Suppressor of RNA polymerase B SRB10
    Gene namesi
    Name:SSN3
    Synonyms:ARE1, CDK8, GIG2, SRB10, UME5
    Ordered Locus Names:YPL042C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL042c.
    SGDiS000005963. SSN3.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi183 – 1831K → R in UME5-4; loss of activity. 1 Publication
    Mutagenesisi304 – 3041D → A: Abrogates kinase activity and transcriptional repression. 7 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 555555Meiotic mRNA stability protein kinase SSN3PRO_0000086784Add
    BLAST

    Proteomic databases

    MaxQBiP39073.
    PaxDbiP39073.

    Expressioni

    Inductioni

    Protein level and kinase activity are reduced during nitrogen starvation.2 Publications

    Gene expression databases

    GenevestigatoriP39073.

    Interactioni

    Subunit structurei

    Component of the SRB8-11 complex which consists of SRB8, SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair also associate with the RNA polymerase II holoenzyme. Interacts with TUP1.6 Publications

    Protein-protein interaction databases

    BioGridi36137. 263 interactions.
    DIPiDIP-2574N.
    IntActiP39073. 24 interactions.
    MINTiMINT-420230.
    STRINGi4932.YPL042C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39073.
    SMRiP39073. Positions 150-515.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini75 – 463389Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000064012.
    HOGENOMiHOG000233024.
    KOiK02208.
    OMAiFHSHPEK.
    OrthoDBiEOG7K3TWD.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39073-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYNGKDRAQN SYQPMYQRPM QVQGQQQAQS FVGKKNTIGS VHGKAPMLMA    50
    NNDVFTIGPY RARKDRMRVS VLEKYEVIGY IAAGTYGKVY KAKRQINSGT 100
    NSANGSSLNG TNAKIPQFDS TQPKSSSSMD MQANTNALRR NLLKDEGVTP 150
    GRIRTTREDV SPHYNSQKQT LIKKPLTVFY AIKKFKTEKD GVEQLHYTGI 200
    SQSACREMAL CRELHNKHLT TLVEIFLERK CVHMVYEYAE HDLLQIIHFH 250
    SHPEKRMIPP RMVRSIMWQL LDGVSYLHQN WVLHRDLKPA NIMVTIDGCV 300
    KIGDLGLARK FHNMLQTLYT GDKVVVTIWY RAPELLLGAR HYTPAVDLWS 350
    VGCIFAELIG LQPIFKGEEA KLDSKKTVPF QVNQLQRILE VLGTPDQKIW 400
    PYLEKYPEYD QITKFPKYRD NLATWYHSAG GRDKHALSLL YHLLNYDPIK 450
    RIDAFNALEH KYFTESDIPV SENVFEGLTY KYPARRIHTN DNDIMNLGSR 500
    TKNNTQASGI TAGAAANALG GLGVNRRILA AAAAAAAAVS GNNASDEPSR 550
    KKNRR 555
    Length:555
    Mass (Da):62,847
    Last modified:September 5, 2006 - v3
    Checksum:iC7B6DDC868CCD61E
    GO

    Sequence cautioni

    The sequence AAC13785.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27151 Genomic DNA. Translation: AAA35193.1.
    U20222 Genomic DNA. Translation: AAC13785.1. Different initiation.
    U44030 Genomic DNA. Translation: AAB68178.1.
    BK006949 Genomic DNA. Translation: DAA11387.1.
    PIRiS50157.
    RefSeqiNP_015283.1. NM_001183856.1.

    Genome annotation databases

    EnsemblFungiiYPL042C; YPL042C; YPL042C.
    GeneIDi856065.
    KEGGisce:YPL042C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27151 Genomic DNA. Translation: AAA35193.1 .
    U20222 Genomic DNA. Translation: AAC13785.1 . Different initiation.
    U44030 Genomic DNA. Translation: AAB68178.1 .
    BK006949 Genomic DNA. Translation: DAA11387.1 .
    PIRi S50157.
    RefSeqi NP_015283.1. NM_001183856.1.

    3D structure databases

    ProteinModelPortali P39073.
    SMRi P39073. Positions 150-515.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36137. 263 interactions.
    DIPi DIP-2574N.
    IntActi P39073. 24 interactions.
    MINTi MINT-420230.
    STRINGi 4932.YPL042C.

    Chemistry

    BindingDBi P39073.
    ChEMBLi CHEMBL5395.

    Proteomic databases

    MaxQBi P39073.
    PaxDbi P39073.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL042C ; YPL042C ; YPL042C .
    GeneIDi 856065.
    KEGGi sce:YPL042C.

    Organism-specific databases

    CYGDi YPL042c.
    SGDi S000005963. SSN3.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000064012.
    HOGENOMi HOG000233024.
    KOi K02208.
    OMAi FHSHPEK.
    OrthoDBi EOG7K3TWD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33956-MONOMER.
    BRENDAi 2.7.11.22. 984.

    Miscellaneous databases

    NextBioi 981047.
    PROi P39073.

    Gene expression databases

    Genevestigatori P39073.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast UME5 gene regulates the stability of meiotic mRNAs in response to glucose."
      Surosky R.T., Strich R., Esposito R.E.
      Mol. Cell. Biol. 14:3446-3458(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF LYS-183.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH SSN8.
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: FUNCTION, INDUCTION.
    6. "Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases."
      Hengartner C.J., Myer V.E., Liao S.-M., Wilson C.J., Koh S.S., Young R.A.
      Mol. Cell 2:43-53(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH SSN8 AND THE RNA POLYMERASE II HOLOENZYME, MUTAGENESIS OF ASP-304.
    7. "GAL4 is regulated by the RNA polymerase II holoenzyme-associated cyclin-dependent protein kinase SRB10/CDK8."
      Hirst M., Kobor M.S., Kuriakose N., Greenblatt J., Sadowski I.
      Mol. Cell 3:673-678(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-304.
    8. "Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase."
      Chi Y., Huddleston M.J., Zhang X., Young R.A., Annan R.S., Carr S.A., Deshaies R.J.
      Genes Dev. 15:1078-1092(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-304.
    9. "The structural and functional organization of the yeast mediator complex."
      Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
      J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED1 AND MED4, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    10. "Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression."
      Zaman Z., Ansari A.Z., Koh S.S., Young R., Ptashne M.
      Proc. Natl. Acad. Sci. U.S.A. 98:2550-2554(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SSN8 AND TUP1, MUTAGENESIS OF ASP-304.
    11. "A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast."
      Borggrefe T., Davis R., Erdjument-Bromage H., Tempst P., Kornberg R.D.
      J. Biol. Chem. 277:44202-44207(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SRB8-11 COMPLEX, FUNCTION OF THE SRB8-11 COMPLEX, CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-304.
    12. "Srb10/Cdk8 regulates yeast filamentous growth by phosphorylating the transcription factor Ste12."
      Nelson C., Goto S., Lund K., Hung W., Sadowski I.
      Nature 421:187-190(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, INDUCTION, MUTAGENESIS OF ASP-304.
    13. Cited for: NOMENCLATURE.
    14. "Two cyclin-dependent kinases promote RNA polymerase II transcription and formation of the scaffold complex."
      Liu Y., Kung C., Fishburn J., Ansari A.Z., Shokat K.M., Hahn S.
      Mol. Cell. Biol. 24:1721-1735(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH RNA POLYMERASE II.
    15. "Promoter-dependent roles for the Srb10 cyclin-dependent kinase and the Hda1 deacetylase in Tup1-mediated repression in Saccharomyces cerevisiae."
      Green S.R., Johnson A.D.
      Mol. Biol. Cell 15:4191-4202(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASP-304.
    16. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
    17. "Site-specific Srb10-dependent phosphorylation of the yeast Mediator subunit Med2 regulates gene expression from the 2-micrometer plasmid."
      Hallberg M., Polozkov G.V., Hu G.-Z., Beve J., Gustafsson C.M., Ronne H., Bjoerklund S.
      Proc. Natl. Acad. Sci. U.S.A. 101:3370-3375(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    18. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
      Nair D., Kim Y., Myers L.C.
      J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    19. "Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
      van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
      Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    20. "The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription."
      Larschan E., Winston F.
      Mol. Cell. Biol. 25:114-123(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE SRB8-11 COMPLEX.
    21. "Transcriptional activating regions target attached substrates to a cyclin-dependent kinase."
      Ansari A.Z., Ogirala A., Ptashne M.
      Proc. Natl. Acad. Sci. U.S.A. 102:2346-2349(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
    22. "Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA."
      Andrau J.-C., van de Pasch L., Lijnzaad P., Bijma T., Koerkamp M.G., van de Peppel J., Werner M., Holstege F.C.P.
      Mol. Cell 22:179-192(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSSN3_YEAST
    AccessioniPrimary (citable) accession number: P39073
    Secondary accession number(s): D6W3X1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 145 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3