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Protein

Meiotic mRNA stability protein kinase SSN3

Gene

SSN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions. Phosphorylates GCN4, promoting its ubiquitin-mediated degradation, and MSN2, promoting its nuclear exclusion. Phosphorylates STE12, thereby promoting its degradation and inhibition of filamentous growth. Phosphorylates GAL4, and this phosphorylation is required for efficient galactose-inducible transcription. Also phosphorylates BDF1 and the TAF2 subunit of the TFIID complex.17 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.7 Publications
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei183ATPCurated1
Active sitei286Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi81 – 89ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II carboxy-terminal domain kinase activity Source: SGD

GO - Biological processi

  • meiotic cell cycle Source: UniProtKB-KW
  • negative regulation of filamentous growth Source: SGD
  • negative regulation of transcription by RNA polymerase II Source: SGD
  • nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD
  • protein destabilization Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of mitotic cell cycle Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase
Biological processMeiosis, Transcription, Transcription regulation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33956-MONOMER
BRENDAi2.7.11.22 984

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic mRNA stability protein kinase SSN3 (EC:2.7.11.227 Publications, EC:2.7.11.237 Publications)
Alternative name(s):
Cyclin-dependent kinase 8
Suppressor of RNA polymerase B SRB10
Gene namesi
Name:SSN3
Synonyms:ARE1, CDK8, GIG2, SRB10, UME5
Ordered Locus Names:YPL042C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL042C
SGDiS000005963 SSN3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi183K → R in UME5-4; loss of activity. 1 Publication1
Mutagenesisi304D → A: Abrogates kinase activity and transcriptional repression. 7 Publications1

Chemistry databases

ChEMBLiCHEMBL5395

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867841 – 555Meiotic mRNA stability protein kinase SSN3Add BLAST555

Proteomic databases

MaxQBiP39073
PaxDbiP39073
PRIDEiP39073

PTM databases

iPTMnetiP39073

Expressioni

Inductioni

Protein level and kinase activity are reduced during nitrogen starvation.2 Publications

Interactioni

Subunit structurei

Component of the SRB8-11 complex which consists of SRB8, SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair also associate with the RNA polymerase II holoenzyme. Interacts with TUP1.6 Publications

Protein-protein interaction databases

BioGridi36137, 378 interactors
DIPiDIP-2574N
IntActiP39073, 25 interactors
MINTiP39073
STRINGi4932.YPL042C

Chemistry databases

BindingDBiP39073

Structurei

3D structure databases

ProteinModelPortaliP39073
SMRiP39073
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini75 – 463Protein kinasePROSITE-ProRule annotationAdd BLAST389

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000064012
HOGENOMiHOG000233024
InParanoidiP39073
KOiK02208
OMAiIMWQILD
OrthoDBiEOG092C2FL8

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P39073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNGKDRAQN SYQPMYQRPM QVQGQQQAQS FVGKKNTIGS VHGKAPMLMA
60 70 80 90 100
NNDVFTIGPY RARKDRMRVS VLEKYEVIGY IAAGTYGKVY KAKRQINSGT
110 120 130 140 150
NSANGSSLNG TNAKIPQFDS TQPKSSSSMD MQANTNALRR NLLKDEGVTP
160 170 180 190 200
GRIRTTREDV SPHYNSQKQT LIKKPLTVFY AIKKFKTEKD GVEQLHYTGI
210 220 230 240 250
SQSACREMAL CRELHNKHLT TLVEIFLERK CVHMVYEYAE HDLLQIIHFH
260 270 280 290 300
SHPEKRMIPP RMVRSIMWQL LDGVSYLHQN WVLHRDLKPA NIMVTIDGCV
310 320 330 340 350
KIGDLGLARK FHNMLQTLYT GDKVVVTIWY RAPELLLGAR HYTPAVDLWS
360 370 380 390 400
VGCIFAELIG LQPIFKGEEA KLDSKKTVPF QVNQLQRILE VLGTPDQKIW
410 420 430 440 450
PYLEKYPEYD QITKFPKYRD NLATWYHSAG GRDKHALSLL YHLLNYDPIK
460 470 480 490 500
RIDAFNALEH KYFTESDIPV SENVFEGLTY KYPARRIHTN DNDIMNLGSR
510 520 530 540 550
TKNNTQASGI TAGAAANALG GLGVNRRILA AAAAAAAAVS GNNASDEPSR

KKNRR
Length:555
Mass (Da):62,847
Last modified:September 5, 2006 - v3
Checksum:iC7B6DDC868CCD61E
GO

Sequence cautioni

The sequence AAC13785 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27151 Genomic DNA Translation: AAA35193.1
U20222 Genomic DNA Translation: AAC13785.1 Different initiation.
U44030 Genomic DNA Translation: AAB68178.1
BK006949 Genomic DNA Translation: DAA11387.1
PIRiS50157
RefSeqiNP_015283.1, NM_001183856.1

Genome annotation databases

EnsemblFungiiYPL042C; YPL042C; YPL042C
GeneIDi856065
KEGGisce:YPL042C

Similar proteinsi

Entry informationi

Entry nameiSSN3_YEAST
AccessioniPrimary (citable) accession number: P39073
Secondary accession number(s): D6W3X1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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