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Protein

ATP-dependent protease subunit ClpQ

Gene

clpQ

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease subunit of a proteasome-like degradation complex.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei21
Metal bindingi165Sodium; via carbonyl oxygen1
Metal bindingi168Sodium; via carbonyl oxygen1
Metal bindingi171Sodium; via carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Metal-binding, Sodium

Enzyme and pathway databases

BioCyciBSUB:BSU16150-MONOMER.

Protein family/group databases

MEROPSiT01.007.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent protease subunit ClpQ (EC:3.4.21.-)
Gene namesi
Name:clpQ
Synonyms:codW, hslV
Ordered Locus Names:BSU16150
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2S → A or T: Complete loss of protease activity. 1 Publication1
Mutagenesisi6A → G: No effect. 1 Publication1
Mutagenesisi7T → A: Complete loss of protease activity. The mutant is only found as a monomer; when associated with A-8. 1 Publication1
Mutagenesisi8T → A: Complete loss of protease activity. The mutant is only found as a monomer; when associated with A-7. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000266782 – 181ATP-dependent protease subunit ClpQAdd BLAST180

Proteomic databases

PaxDbiP39070.

Interactioni

Subunit structurei

A double ring-shaped homohexamer of ClpQ is capped on each side by a ring-shaped ClpY homohexamer. The assembly of the ClpQ/ClpY complex is dependent on binding of ATP (By similarity).By similarity

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008896.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 14Combined sources6
Beta strandi17 – 22Combined sources6
Beta strandi26 – 28Combined sources3
Turni29 – 31Combined sources3
Beta strandi32 – 36Combined sources5
Beta strandi41 – 44Combined sources4
Turni45 – 48Combined sources4
Beta strandi49 – 53Combined sources5
Helixi57 – 73Combined sources17
Turni74 – 76Combined sources3
Helixi78 – 91Combined sources14
Helixi95 – 97Combined sources3
Beta strandi102 – 105Combined sources4
Beta strandi110 – 113Combined sources4
Beta strandi123 – 130Combined sources8
Helixi133 – 147Combined sources15
Helixi148 – 150Combined sources3
Helixi153 – 167Combined sources15
Beta strandi176 – 180Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YYFX-ray4.16C/D1-181[»]
2Z3AX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L2-181[»]
2Z3BX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L2-181[»]
ProteinModelPortaliP39070.
SMRiP39070.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39070.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family. HslV subfamily.Curated

Phylogenomic databases

eggNOGiENOG4108R5P. Bacteria.
COG5405. LUCA.
HOGENOMiHOG000064533.
InParanoidiP39070.
KOiK01419.
OMAiIMKGNAR.
PhylomeDBiP39070.

Family and domain databases

CDDicd01913. protease_HslV. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00248. HslV. 1 hit.
InterProiIPR022281. ATP-dep_Prtase_HsIV_su.
IPR029055. Ntn_hydrolases_N.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PIRSFiPIRSF039093. HslV. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03692. ATP_dep_HslV. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFHATTIF AVQHKGRSAM SGDGQVTFGQ AVVMKHTARK VRKLFNGKVL
60 70 80 90 100
AGFAGSVADA FTLFEKFEAK LEEYNGNLKR AAVELAKEWR SDKVLRKLEA
110 120 130 140 150
MLIVMNQDTL LLVSGTGEVI EPDDGILAIG SGGNYALAAG RALKKHAGES
160 170 180
MSASEIARAA LETAGEICVY TNDQIILEEL E
Length:181
Mass (Da):19,478
Last modified:February 1, 1995 - v1
Checksum:i95DC72474A41A97D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 5Missing in CAA83919 (Ref. 2) Curated4
Sequence conflicti60A → R in CAA83919 (Ref. 2) Curated1
Sequence conflicti72E → K in CAA83919 (Ref. 2) Curated1

Mass spectrometryi

Molecular mass is 19345.7 Da from positions 2 - 181. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13634 Genomic DNA. Translation: AAB03370.1.
Z33639 Genomic DNA. Translation: CAA83919.1.
AL009126 Genomic DNA. Translation: CAB13488.1.
PIRiS61494.
RefSeqiNP_389497.1. NC_000964.3.
WP_003238555.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13488; CAB13488; BSU16150.
GeneIDi11239476.
938111.
KEGGibsu:BSU16150.
PATRICi18975035. VBIBacSub10457_1709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13634 Genomic DNA. Translation: AAB03370.1.
Z33639 Genomic DNA. Translation: CAA83919.1.
AL009126 Genomic DNA. Translation: CAB13488.1.
PIRiS61494.
RefSeqiNP_389497.1. NC_000964.3.
WP_003238555.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YYFX-ray4.16C/D1-181[»]
2Z3AX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L2-181[»]
2Z3BX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L2-181[»]
ProteinModelPortaliP39070.
SMRiP39070.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008896.

Protein family/group databases

MEROPSiT01.007.

Proteomic databases

PaxDbiP39070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13488; CAB13488; BSU16150.
GeneIDi11239476.
938111.
KEGGibsu:BSU16150.
PATRICi18975035. VBIBacSub10457_1709.

Phylogenomic databases

eggNOGiENOG4108R5P. Bacteria.
COG5405. LUCA.
HOGENOMiHOG000064533.
InParanoidiP39070.
KOiK01419.
OMAiIMKGNAR.
PhylomeDBiP39070.

Enzyme and pathway databases

BioCyciBSUB:BSU16150-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP39070.

Family and domain databases

CDDicd01913. protease_HslV. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00248. HslV. 1 hit.
InterProiIPR022281. ATP-dep_Prtase_HsIV_su.
IPR029055. Ntn_hydrolases_N.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PIRSFiPIRSF039093. HslV. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03692. ATP_dep_HslV. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPQ_BACSU
AccessioniPrimary (citable) accession number: P39070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.