Reviewed, UniProtKB/Swiss-Prot P39069 (KAD1_RAT)

Names and origin

Protein names

Recommended name:
    Adenylate kinase isoenzyme 1
      Short name=AK 1
    EC=2.7.4.3
Alternative name(s):
    ATP-AMP transphosphorylase 1
    Myokinase

Gene names

Name:

Ak1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
Catalytic activity	ATP + AMP = 2 ADP.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the adenylate kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP metabolic process Inferred from electronic annotation. Source: InterPro adenine metabolic process Ref.4 Non-traceable author statement. Source: RGD positive regulation of neuron maturation Inferred from expression pattern. Source: RGD
Cellular component	neuron projection Inferred from direct assay. Source: RGD perinuclear region of cytoplasm Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Ref.4 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Adenylate kinase isoenzyme 1		PRO_0000158914
Regions
	Nucleotide binding	15 – 23	9	ATP By similarity
	Nucleotide binding	94 – 101	8	AMP By similarity
Sites
	Binding site	39	1	AMP By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
Experimental info
	Sequence conflict	81	1	L → F in BAA97655. Ref.1
	Sequence conflict	104	1	E → S in BAA02643. Ref.4
	Sequence conflict	175	1	A → L in BAA02643. Ref.4
	Sequence conflict	179	1	V → W in BAA02643. Ref.4
	Sequence conflict	187	1	C → S in BAA97655. Ref.1
	Sequence conflict	187	1	C → S in BAA02643. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P39069-1 [UniParc]. Last modified January 9, 2007. Version 3. Checksum: 4D53BCB3EF8B7629	FASTA	194	21,584
	10 20 30 40 50 60 MEDKLKKAKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRAEVSSG SSRGKMLSSI 70 80 90 100 110 120 MEKGELVPLE TVLDMLRDAM LAKVDSSNGF LIDGYPREVK QGEEFERKIA QPTLLLYVDA 130 140 150 160 170 180 GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV ISFYDKRGIV RKVNAEGSVD 190 TVFSQVCTYL DSLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat adenylate kinase isozyme 1." Noma T. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	Lubec G., Afjehi-Sadat L., Diao W., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 10-21; 32-44; 64-77; 84-97; 101-128; 156-167 AND 172-194, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain, Hippocampus and Spinal cord.
[4]	"Tissue-specific and developmentally regulated expression of the genes encoding adenylate kinase isozymes." Tanabe T., Yamada M., Noma T., Kajii T., Nakazawa A. J. Biochem. 113:200-207(1993) [PubMed: 8468325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 72-194.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB022701 mRNA. Translation: BAA97655.1. BC089797 mRNA. Translation: AAH89797.1. D13376 mRNA. Translation: BAA02643.1.
IPI	IPI00210351.
PIR	PQ0534.
RefSeq	NP_077325.2.
UniGene	Rn.105715 Rn.79537
3D structure databases
SMR	P39069. Positions 1-194.
ModBase	Search...
Genome annotation databases
GeneID	24183.
KEGG	rno:24183.
Organism-specific databases
CTD	24183.
RGD	2076. Ak1.
Phylogenomic databases
HOVERGEN	P39069.
Enzyme and pathway databases
BRENDA	2.7.4.3. 248.
Gene expression databases
Genevestigator	P39069.
Family and domain databases
InterPro	IPR000850. Adenylate_kin. IPR006267. Adenylate_kin1. [Graphical view]
PANTHER	PTHR23359. Adenylate_kin. 1 hit.
Pfam	PF00406. ADK. 1 hit. [Graphical view]
PRINTS	PR00094. ADENYLTKNASE.
TIGRFAMs	TIGR01360. aden_kin_iso1. 1 hit.
PROSITE	PS00113. ADENYLATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	602533.

Entry information

Entry name

KAD1_RAT

Accession

Primary (citable) accession number: P39069
Secondary accession number(s): Q5EBC5, Q9JJN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 9, 2007
Last modified:	February 9, 2010
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)