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Protein

Dynamin

Gene

dyn-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis (By similarity). Required for coelomocyte endocytosis (PubMed:20803083). Involved in apoptotic cell phagocytosis (PubMed:21490059). Required for recruitment of phosphatidylinositol 3-kinase piki-1 to phagosomes (PubMed:22272187). May play a role in rab-5 recruitment to cell-corpses-containing phagosomes but not to endosomes (PubMed:18425118). Required for embryonic and larval development (PubMed:9294229, PubMed:21490059, PubMed:20803083).By similarity4 Publications

Catalytic activityi

GTP + H2O = GDP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 48GTPBy similarity9
Nucleotide bindingi207 – 213GTPBy similarity7
Nucleotide bindingi238 – 241GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: WormBase
  • GTP binding Source: UniProtKB-KW
  • microtubule binding Source: GO_Central

GO - Biological processi

  • dynamin family protein polymerization involved in mitochondrial fission Source: GO_Central
  • embryo development ending in birth or egg hatching Source: WormBase
  • endocytosis Source: WormBase
  • locomotion Source: WormBase
  • membrane fusion Source: GO_Central
  • mitochondrial fission Source: GO_Central
  • phagosome maturation involved in apoptotic cell clearance Source: WormBase
  • positive regulation of necrotic cell death Source: WormBase
  • receptor-mediated endocytosis Source: WormBase

Keywordsi

Molecular functionHydrolase, Motor protein
Biological processEndocytosis, Phagocytosis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.5 1045
ReactomeiR-CEL-1169408 ISG15 antiviral mechanism
R-CEL-437239 Recycling pathway of L1
R-CEL-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin (EC:3.6.5.5)
Gene namesi
Name:dyn-1
ORF Names:C02C6.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC02C6.1a ; CE07833 ; WBGene00001130 ; dyn-1
C02C6.1b ; CE07832 ; WBGene00001130 ; dyn-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes abnormal accumulation of apoptotic cell corpses in early phagosomes in gonads.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi70P → S in dy51; temperature sensitive mutant which at the restrictive temperature of 25 degrees Celsius displays uncoordinated movements, reduced pharyngeal pumping rate, prolonged defecation cycle, an egg-laying defect and embryonic lethality of the progeny. Accumulates of apoptotic cell corpses in abnormally enlarged early phagosomes prior to rab-5 recruitment. Decreases rab-5 recruitment to phagosomes. Impaired coelomocyte endocytosis. 3 Publications1
Mutagenesisi204G → E in n4039; loss of apoptotic cell engulfment. Embryos fail to hatch. Loss of piki-1 recruitment to the nascent phagosome during apoptotic cell corpse engulfment. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002065751 – 830DynaminAdd BLAST830

Proteomic databases

PaxDbiP39055
PeptideAtlasiP39055
PRIDEiP39055

Expressioni

Tissue specificityi

Expressed in motor neurons in the head and in ventral nerve cord and, to a lesser extent, in sensory neurons in the nerve ring and the tail and interneurons. Expressed in pharyngeal-intestinal valve, intestinal-rectal valve and in intestinal cells.1 Publication

Gene expression databases

BgeeiWBGene00001130
ExpressionAtlasiP39055 baseline and differential

Interactioni

Subunit structurei

May be a component of a complex composed of rab-5 (in GDP-bound form), dyn-1 and vps-34 (PubMed:18425118). Interacts with tax-6 (PubMed:20803083).1 Publication1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi46538, 5 interactors
IntActiP39055, 20 interactors
MINTiP39055
STRINGi6239.C02C6.1b

Structurei

3D structure databases

ProteinModelPortaliP39055
SMRiP39055
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 296Dynamin-type GAdd BLAST267
Domaini519 – 624PHPROSITE-ProRule annotationAdd BLAST106
Domaini653 – 744GEDPROSITE-ProRule annotationAdd BLAST92

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0446 Eukaryota
COG0699 LUCA
GeneTreeiENSGT00760000119213
HOGENOMiHOG000161069
InParanoidiP39055
KOiK01528
OMAiANRYIPE
OrthoDBiEOG091G0EIQ
PhylomeDBiP39055

Family and domain databases

CDDicd08771 DLP_1, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000375 Dynamin_central
IPR001401 Dynamin_GTPase
IPR019762 Dynamin_GTPase_CS
IPR022812 Dynamin_SF
IPR030381 G_DYNAMIN_dom
IPR003130 GED
IPR020850 GED_dom
IPR027417 P-loop_NTPase
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR11566 PTHR11566, 1 hit
PfamiView protein in Pfam
PF01031 Dynamin_M, 1 hit
PF00350 Dynamin_N, 1 hit
PF02212 GED, 1 hit
PF00169 PH, 1 hit
PRINTSiPR00195 DYNAMIN
SMARTiView protein in SMART
SM00053 DYNc, 1 hit
SM00302 GED, 1 hit
SM00233 PH, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00410 G_DYNAMIN_1, 1 hit
PS51718 G_DYNAMIN_2, 1 hit
PS51388 GED, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P39055-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSWQNQGMQA LIPVINRVQD AFSQLGTSVS FELPQIAVVG GQSAGKSSVL
60 70 80 90 100
ENFVGKDFLP RGSGIVTRRP LILQLIQDRN EYAEFLHKKG HRFVDFDAVR
110 120 130 140 150
KEIEDETDRV TGQNKGISPH PINLRVFSPN VLNLTLIDLP GLTKVPVGDQ
160 170 180 190 200
PADIEQQIRD MILTFINRET CLILAVTPAN SDLATSDALK LAKEVDPQGL
210 220 230 240 250
RTIGVLTKLD LMDEGTDARE ILENKLFTLR RGYVGVVNRG QKDIVGRKDI
260 270 280 290 300
RAALDAERKF FISHPSYRHM ADRLGTSYLQ HTLNQQLTNH IRDTLPTLRD
310 320 330 340 350
SLQKKMFAME KDVAEYKNYQ PNDPGRKTKA LLQMVTQFNA DIERSIEGSS
360 370 380 390 400
AKLVSTNELS GGARINRLFH ERFPFEIVKM EIDEKEMRKE IQYAIRNIHG
410 420 430 440 450
IRVGLFTPDM AFEAIAKKQI TRLKEPSLKC VDLVVNELAN VIRQCADTMA
460 470 480 490 500
RYPRLRDELE RIVVSHMRER EQIAKQQIGL IVDYELAYMN TNHEDFIGFS
510 520 530 540 550
NAEAKASQGQ SAKKNLGNQV IRKGWLSLSN VSFVRGSKDN WFVLMSDSLS
560 570 580 590 600
WYKDDEEKEK KYMLPLDGVK LKDIEGGFMS RNHKFALFYP DGKNIYKDYK
610 620 630 640 650
QLELGCTNLD EIDAWKASFL RAGVYPEKQK AQEDESQQEM EDTSIDPQLE
660 670 680 690 700
RQVETIRNLV DSYMRIITKT IKDLVPKAVM HLIVNQTGEF MKDELLAHLY
710 720 730 740 750
QCGDTDALME ESQIEAQKRE EMLRMYHACK EALRIISEVN MSTLGDQPPP
760 770 780 790 800
LPMSDYRPHP SGPSPVPRPA PAPPGGRQAP MPPRGGPGAP PPPGMRPPPG
810 820 830
APGGGGGMYP PLIPTRPGPG GPPPNMAPPR
Length:830
Mass (Da):93,407
Last modified:May 27, 2002 - v3
Checksum:iFF681250E51AB8A5
GO
Isoform b (identifier: P39055-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     817-830: PGPGGPPPNMAPPR → VPTPSNGAPEIPARPQVPKRPF

Note: No experimental confirmation available.
Show »
Length:838
Mass (Da):94,421
Checksum:i78F89B6EA555B689
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti734R → P in AAB72228 (PubMed:9294229).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001329817 – 830PGPGG…MAPPR → VPTPSNGAPEIPARPQVPKR PF in isoform b. CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29031 mRNA Translation: AAB72228.2
Z79596 Genomic DNA Translation: CAB01857.1
Z79596 Genomic DNA Translation: CAC42251.1
PIRiT18860
RefSeqiNP_001024331.1, NM_001029160.2 [P39055-1]
NP_001024332.1, NM_001029161.2
UniGeneiCel.19561

Genome annotation databases

EnsemblMetazoaiC02C6.1a; C02C6.1a; WBGene00001130 [P39055-1]
GeneIDi181644
KEGGicel:CELE_C02C6.1
UCSCiC02C6.1b c. elegans [P39055-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDYN1_CAEEL
AccessioniPrimary (citable) accession number: P39055
Secondary accession number(s): Q93176, Q95QY9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 27, 2002
Last modified: March 28, 2018
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health