P39055 (DYN1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dynamin
EC=3.6.5.5

Gene names

Name:	dyn-1
ORF Names:	C02C6.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	830 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
Catalytic activity	GTP + H2O = GDP + phosphate.
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton. Note: Microtubule-associated.
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Motor protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase engulfment of apoptotic cell Inferred from mutant phenotype. Source: WormBase germ cell development Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase inductive cell migration Inferred from mutant phenotype. Source: WormBase molting cycle, collagen and cuticulin-based cuticle Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase phagosome maturation involved in apoptotic cell clearance Inferred from mutant phenotype. Source: WormBase protein catabolic process Inferred from mutant phenotype. Source: WormBase receptor-mediated endocytosis Inferred from mutant phenotype. Source: WormBase
Cellular component	apical part of cell Inferred from direct assay. Source: WormBase cleavage furrow Inferred from direct assay. Source: WormBase midbody Inferred from direct assay. Source: WormBase phagocytic cup Inferred from direct assay. Source: WormBase phagocytic vesicle Inferred from direct assay. Source: WormBase pseudopodium Inferred from direct assay. Source: WormBase spindle microtubule Inferred from direct assay. Source: WormBase synapse Inferred from direct assay. Source: WormBase
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay. Source: WormBase protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
gei-18	Q7K7J0	3	EBI-317945,EBI-2315822
lst-4	Q8I4E1	2	EBI-317945,EBI-4325777

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P39055-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: P39055-2)

The sequence of this isoform differs from the canonical sequence as follows:
     817-830: PGPGGPPPNMAPPR → VPTPSNGAPEIPARPQVPKRPF

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 830	830	Dynamin		PRO_0000206575
Regions
	Domain	519 – 624	106	PH
	Domain	653 – 744	92	GED
	Nucleotide binding	40 – 47	8	GTP By similarity
	Nucleotide binding	138 – 142	5	GTP By similarity
	Nucleotide binding	207 – 210	4	GTP By similarity
Natural variations
	Alternative sequence	817 – 830	14	PGPGG…MAPPR → VPTPSNGAPEIPARPQVPKR PF in isoform b.		VSP_001329
Experimental info
	Sequence conflict	734	1	R → P in AAB72228. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified May 27, 2002. Version 3. Checksum: FF681250E51AB8A5	FASTA	830	93,407
	10 20 30 40 50 60 MSWQNQGMQA LIPVINRVQD AFSQLGTSVS FELPQIAVVG GQSAGKSSVL ENFVGKDFLP 70 80 90 100 110 120 RGSGIVTRRP LILQLIQDRN EYAEFLHKKG HRFVDFDAVR KEIEDETDRV TGQNKGISPH 130 140 150 160 170 180 PINLRVFSPN VLNLTLIDLP GLTKVPVGDQ PADIEQQIRD MILTFINRET CLILAVTPAN 190 200 210 220 230 240 SDLATSDALK LAKEVDPQGL RTIGVLTKLD LMDEGTDARE ILENKLFTLR RGYVGVVNRG 250 260 270 280 290 300 QKDIVGRKDI RAALDAERKF FISHPSYRHM ADRLGTSYLQ HTLNQQLTNH IRDTLPTLRD 310 320 330 340 350 360 SLQKKMFAME KDVAEYKNYQ PNDPGRKTKA LLQMVTQFNA DIERSIEGSS AKLVSTNELS 370 380 390 400 410 420 GGARINRLFH ERFPFEIVKM EIDEKEMRKE IQYAIRNIHG IRVGLFTPDM AFEAIAKKQI 430 440 450 460 470 480 TRLKEPSLKC VDLVVNELAN VIRQCADTMA RYPRLRDELE RIVVSHMRER EQIAKQQIGL 490 500 510 520 530 540 IVDYELAYMN TNHEDFIGFS NAEAKASQGQ SAKKNLGNQV IRKGWLSLSN VSFVRGSKDN 550 560 570 580 590 600 WFVLMSDSLS WYKDDEEKEK KYMLPLDGVK LKDIEGGFMS RNHKFALFYP DGKNIYKDYK 610 620 630 640 650 660 QLELGCTNLD EIDAWKASFL RAGVYPEKQK AQEDESQQEM EDTSIDPQLE RQVETIRNLV 670 680 690 700 710 720 DSYMRIITKT IKDLVPKAVM HLIVNQTGEF MKDELLAHLY QCGDTDALME ESQIEAQKRE 730 740 750 760 770 780 EMLRMYHACK EALRIISEVN MSTLGDQPPP LPMSDYRPHP SGPSPVPRPA PAPPGGRQAP 790 800 810 820 830 MPPRGGPGAP PPPGMRPPPG APGGGGGMYP PLIPTRPGPG GPPPNMAPPR
	Isoform b [UniParc]. Checksum: 78F89B6EA555B689	FASTA	838	94,421
	10 20 30 40 50 60 MSWQNQGMQA LIPVINRVQD AFSQLGTSVS FELPQIAVVG GQSAGKSSVL ENFVGKDFLP 70 80 90 100 110 120 RGSGIVTRRP LILQLIQDRN EYAEFLHKKG HRFVDFDAVR KEIEDETDRV TGQNKGISPH 130 140 150 160 170 180 PINLRVFSPN VLNLTLIDLP GLTKVPVGDQ PADIEQQIRD MILTFINRET CLILAVTPAN 190 200 210 220 230 240 SDLATSDALK LAKEVDPQGL RTIGVLTKLD LMDEGTDARE ILENKLFTLR RGYVGVVNRG 250 260 270 280 290 300 QKDIVGRKDI RAALDAERKF FISHPSYRHM ADRLGTSYLQ HTLNQQLTNH IRDTLPTLRD 310 320 330 340 350 360 SLQKKMFAME KDVAEYKNYQ PNDPGRKTKA LLQMVTQFNA DIERSIEGSS AKLVSTNELS 370 380 390 400 410 420 GGARINRLFH ERFPFEIVKM EIDEKEMRKE IQYAIRNIHG IRVGLFTPDM AFEAIAKKQI 430 440 450 460 470 480 TRLKEPSLKC VDLVVNELAN VIRQCADTMA RYPRLRDELE RIVVSHMRER EQIAKQQIGL 490 500 510 520 530 540 IVDYELAYMN TNHEDFIGFS NAEAKASQGQ SAKKNLGNQV IRKGWLSLSN VSFVRGSKDN 550 560 570 580 590 600 WFVLMSDSLS WYKDDEEKEK KYMLPLDGVK LKDIEGGFMS RNHKFALFYP DGKNIYKDYK 610 620 630 640 650 660 QLELGCTNLD EIDAWKASFL RAGVYPEKQK AQEDESQQEM EDTSIDPQLE RQVETIRNLV 670 680 690 700 710 720 DSYMRIITKT IKDLVPKAVM HLIVNQTGEF MKDELLAHLY QCGDTDALME ESQIEAQKRE 730 740 750 760 770 780 EMLRMYHACK EALRIISEVN MSTLGDQPPP LPMSDYRPHP SGPSPVPRPA PAPPGGRQAP 790 800 810 820 830 MPPRGGPGAP PPPGMRPPPG APGGGGGMYP PLIPTRVPTP SNGAPEIPAR PQVPKRPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A dynamin GTPase mutation causes a rapid and reversible temperature-inducible locomotion defect in C. elegans." Clark S.G., Shurland D.L., Meyerowitz E.M., Bargmann C.I., van der Bliek A.M. Proc. Natl. Acad. Sci. U.S.A. 94:10438-10443(1997) [PubMed: 9294229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Strain: Bristol N2.
[2]	van der Bliek A.M. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS A AND B). Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L29031 mRNA. Translation: AAB72228.2. Z79596 Genomic DNA. Translation: CAB01857.1. Z79596 Genomic DNA. Translation: CAC42251.1.
PIR	T18860.
RefSeq	NP_001024331.1. NM_001029160.1. NP_001024332.1. NM_001029161.1.
UniGene	Cel.19561.
3D structure databases
ProteinModelPortal	P39055.
SMR	P39055. Positions 8-740.
ModBase	Search...
Protein-protein interaction databases
IntAct	P39055. 8 interactions.
MINT	MINT-230275.
STRING	P39055.
Proteomic databases
PRIDE	P39055.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C02C6.1a; C02C6.1a; C02C6.1.
GeneID	181644.
KEGG	cel:C02C6.1.
UCSC	C02C6.1b. c. elegans.
Organism-specific databases
CTD	181644.
WormBase	C02C6.1a; CE07833; WBGene00001130; dyn-1. C02C6.1b; CE07832; WBGene00001130; dyn-1.
Phylogenomic databases
eggNOG	meNOG04077.
GeneTree	EMGT00050000000104.
InParanoid	P39055.
OMA	DMILTFI.
PhylomeDB	P39055.
Gene expression databases
ArrayExpress	P39055.
Family and domain databases
InterPro	IPR022812. Dynamin. IPR000375. Dynamin_central. IPR001401. Dynamin_GTPase. IPR019762. Dynamin_GTPase_CS. IPR003130. GED. IPR020850. GTPase_effector_domain_GED. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 2 hits.
KO	K01528.
Pfam	PF01031. Dynamin_M. 1 hit. PF00350. Dynamin_N. 1 hit. PF02212. GED. 1 hit. PF00169. PH. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
SMART	SM00053. DYNc. 1 hit. SM00302. GED. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS00410. DYNAMIN. 1 hit. PS51388. GED. 1 hit. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	914798.

Entry information

Entry name

DYN1_CAEEL

Accession

Primary (citable) accession number: P39055
Secondary accession number(s): Q93176, Q95QY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	May 27, 2002
Last modified:	January 25, 2012
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project