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P39054 (DYN2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dynamin-2

EC=3.6.5.5
Alternative name(s):
Dynamin UDNM
Gene names
Name:Dnm2
Synonyms:Dyn2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length870 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis. Ref.6

Catalytic activity

GTP + H2O = GDP + phosphate.

Subunit structure

Interacts with SHANK1, SHANK2 and NOSTRIN By similarity. Interacts with SNX9. Interacts with SNX33 (via SH3 domain). Interacts with MYO1E (via SH3 domain) By similarity. Interacts with MYOF and SH3BP4. Interacts with PSTPIP1 By similarity. Ref.4 Ref.5

Subcellular location

Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity. Cell junctionsynapse By similarity. Midbody By similarity. Note: Microtubule-associated. Also found in the postsynaptic density of neuronal cells By similarity.

Tissue specificity

Expressed in most tissues during embryonic development, including the peripheral nervous system although no expression is evident in skeletal muscle or heart. Ref.7

Post-translational modification

Phosphorylation at Ser-764 by CDK1 is greatly increased upon mitotic entry. It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis. Dephosphorylated by Calcineurin/PP2 By similarity.

Disruption phenotype

Exhibits growth and cytokinesis defects. Ref.6

Sequence similarities

Belongs to the dynamin family.

Contains 1 GED domain.

Contains 1 PH domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CttnQ605982EBI-642337,EBI-397955
CTTN1Q014062EBI-642337,EBI-2530463From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P39054-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P39054-2)

The sequence of this isoform differs from the canonical sequence as follows:
     516-519: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 870870Dynamin-2
PRO_0000206571

Regions

Domain519 – 625107PH
Domain653 – 74492GED
Nucleotide binding38 – 458GTP By similarity
Nucleotide binding136 – 1405GTP By similarity
Nucleotide binding205 – 2084GTP By similarity
Compositional bias747 – 866120Pro-rich

Amino acid modifications

Modified residue2991N6-acetyllysine Ref.8
Modified residue5981N6-acetyllysine By similarity
Modified residue7641Phosphoserine; by CDK1 By similarity

Natural variations

Alternative sequence516 – 5194Missing in isoform 2.
VSP_001326

Experimental info

Sequence conflict297 – 2982RS → HG in AAA40523. Ref.1
Sequence conflict848 – 87023SRRAP…PSLLD → RRPPPLAPARPFF in BAB23745. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 26, 2002. Version 2.
Checksum: E80864AF94B8F778

FASTA87098,145
        10         20         30         40         50         60 
MGNRGMEELI PLVNKLQDAF SSIGQSCHLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 

        70         80         90        100        110        120 
SGIVTRRPLI LQLIFSKTEY AEFLHCKSKK FTDFDEVRQE IEAETDRVTG TNKGISPVPI 

       130        140        150        160        170        180 
NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIKDMI LQFISRESSL ILAVTPANMD 

       190        200        210        220        230        240 
LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 

       250        260        270        280        290        300 
DIEGKKDIRA ALAAERKFFL SHPAYRHMAD RMGTPHLQKT LNQQLTNHIR ESLPTLRSKL 

       310        320        330        340        350        360 
QSQLLSLEKE VEEYKNFRPD DPTRKTKALL QMVQQFGVDF EKRIEGSGDQ VDTLELSGGA 

       370        380        390        400        410        420 
RINRIFHERF PFELVKMEFD EKDLRREISY AIKNIHGVRT GLFTPDLAFE AIVKKQVVKL 

       430        440        450        460        470        480 
KEPCLKCVDL VIQELISTVR QCTSKLSSYP RLREETERIV TTYIREREGR TKDQILLLID 

       490        500        510        520        530        540 
IEQSYINTNH EDFIGFANAQ QRSTQLNKKR AIPNQGEILV IRRGWLTINN ISLMKGGSKE 

       550        560        570        580        590        600 
YWFVLTAESL SWYKDEEEKE KKYMLPLDNL KIRDVEKGFM SNKHVFAIFN TEQRNVYKDL 

       610        620        630        640        650        660 
RQIELACDSQ EDVDSWKASF LRAGVYPEKD QAENEDGAQE NTFSMDPQLE RQVETIRNLV 

       670        680        690        700        710        720 
DSYVAIINKS IRDLMPKTIM HLMINNTKAF IHHELLAYLY SSADQSSLME ESAEQAQRRD 

       730        740        750        760        770        780 
DMLRMYHALK EALNIIGDIS TSTVSTPVPP PVDDTWLQNT SGHSPTPQRR PVSSVHPPGR 

       790        800        810        820        830        840 
PPAVRGPTPG PPLIPMPVGA TSSFSAPPIP SRPGPQSVFA NNDPFSAPPQ IPSRPARIPP 

       850        860        870 
GIPPGVPSRR APAAPSRPTI IRPAEPSLLD 

« Hide

Isoform 2 [UniParc].

Checksum: CA0BEB6FA5E5028D
Show »

FASTA86697,733

References

« Hide 'large scale' references
[1]"Dynamin genes Dnm1 and Dnm2 are located on proximal mouse chromosomes 2 and 9, respectively."
Klocke R., Augustin A., Ronsiek M., Stief A., van der Putten H., Jockusch H.
Genomics 41:290-292(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: NIH Swiss.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Liver.
[3]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 45-54; 91-107; 114-142; 207-217; 230-237; 300-309; 370-376 AND 678-688, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[4]"TTP specifically regulates the internalization of the transferrin receptor."
Tosoni D., Puri C., Confalonieri S., Salcini A.E., De Camilli P., Tacchetti C., Di Fiore P.P.
Cell 123:875-888(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SH3BP4.
[5]"Myoferlin regulates vascular endothelial growth factor receptor-2 stability and function."
Bernatchez P.N., Acevedo L., Fernandez-Hernando C., Murata T., Chalouni C., Kim J., Erdjument-Bromage H., Shah V., Gratton J.-P., McNally E.M., Tempst P., Sessa W.C.
J. Biol. Chem. 282:30745-30753(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MYOF.
[6]"Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cells."
Liu Y.W., Surka M.C., Schroeter T., Lukiyanchuk V., Schmid S.L.
Mol. Biol. Cell 19:5347-5359(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]"Dynamin 2 homozygous mutation in humans with a lethal congenital syndrome."
Koutsopoulos O.S., Kretz C., Weller C.M., Roux A., Mojzisova H., Boehm J., Koch C., Toussaint A., Heckel E., Stemkens D., Ter Horst S.A., Thibault C., Koch M., Mehdi S.Q., Bijlsma E.K., Mandel J.L., Vermot J., Laporte J.
Eur. J. Hum. Genet. 21:637-642(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L31398 mRNA. Translation: AAA40523.1.
AK005012 mRNA. Translation: BAB23745.1.
RefSeqNP_001240822.1. NM_001253893.1.
NP_001240823.1. NM_001253894.1.
NP_031897.2. NM_007871.2.
UniGeneMm.433257.

3D structure databases

ProteinModelPortalP39054.
SMRP39054. Positions 5-737.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP39054. 15 interactions.
MINTMINT-1605893.

PTM databases

PhosphoSiteP39054.

2D gel databases

REPRODUCTION-2DPAGEIPI00131445.

Proteomic databases

PaxDbP39054.
PRIDEP39054.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000091087; ENSMUSP00000088616; ENSMUSG00000033335. [P39054-2]
ENSMUST00000172482; ENSMUSP00000133564; ENSMUSG00000033335. [P39054-1]
GeneID13430.
KEGGmmu:13430.
UCSCuc009olk.2. mouse. [P39054-2]
uc029wxt.1. mouse. [P39054-1]

Organism-specific databases

CTD1785.
MGIMGI:109547. Dnm2.

Phylogenomic databases

eggNOGCOG0699.
GeneTreeENSGT00740000115253.
HOGENOMHOG000161069.
HOVERGENHBG107833.
InParanoidP39054.
KOK01528.
PhylomeDBP39054.
TreeFamTF300362.

Gene expression databases

ArrayExpressP39054.
BgeeP39054.
CleanExMM_DNM2.
GenevestigatorP39054.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
3.40.50.300. 1 hit.
InterProIPR027188. DNM2.
IPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR003130. GED.
IPR020850. GTPase_effector_domain_GED.
IPR027417. P-loop_NTPase.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PANTHERPTHR11566. PTHR11566. 1 hit.
PTHR11566:SF23. PTHR11566:SF23. 1 hit.
PfamPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSPR00195. DYNAMIN.
SMARTSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00410. DYNAMIN. 1 hit.
PS51388. GED. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDNM2. mouse.
NextBio283855.
PROP39054.
SOURCESearch...

Entry information

Entry nameDYN2_MOUSE
AccessionPrimary (citable) accession number: P39054
Secondary accession number(s): Q9DBE1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 26, 2002
Last modified: April 16, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot