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Protein

Dynamin-2

Gene

Dnm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis (PubMed:18923138). Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane (PubMed:18425118).By similarity2 Publications

Catalytic activityi

GTP + H2O = GDP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469GTPBy similarity
Nucleotide bindingi205 – 2117GTPBy similarity
Nucleotide bindingi236 – 2394GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Motor protein

Keywords - Biological processi

Endocytosis, Phagocytosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.5. 3474.
ReactomeiR-MMU-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-MMU-190873. Gap junction degradation.
R-MMU-196025. Formation of annular gap junctions.
R-MMU-203641. NOSTRIN mediated eNOS trafficking.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-437239. Recycling pathway of L1.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-2 (EC:3.6.5.5)
Alternative name(s):
Dynamin UDNM
Gene namesi
Name:Dnm2
Synonyms:Dyn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:109547. Dnm2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Coated pit, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Exhibits growth and cytokinesis defects.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi44 – 441K → A: Apoptotic cell corpse-containing phagosomes fail to mature into acidic phagosomes. Loss of PIK3C3 and RAB5A recruitment to phagosomes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 870870Dynamin-2PRO_0000206571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei299 – 2991N6-acetyllysineCombined sources
Modified residuei598 – 5981N6-acetyllysineBy similarity
Modified residuei755 – 7551PhosphothreonineBy similarity
Modified residuei764 – 7641Phosphoserine; by CDK1By similarity

Post-translational modificationi

Phosphorylation at Ser-764 by CDK1 is greatly increased upon mitotic entry. It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis. Dephosphorylated by calcineurin/PP2 (By similarity). Phosphorylated on tyrosine residues after activation of SRC (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP39054.
PaxDbiP39054.
PeptideAtlasiP39054.
PRIDEiP39054.

2D gel databases

REPRODUCTION-2DPAGEIPI00131445.

PTM databases

iPTMnetiP39054.
PhosphoSiteiP39054.

Expressioni

Tissue specificityi

Expressed in most tissues during embryonic development, including the peripheral nervous system although no expression is evident in skeletal muscle or heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000033335.
CleanExiMM_DNM2.
ExpressionAtlasiP39054. baseline and differential.
GenevisibleiP39054. MM.

Interactioni

Subunit structurei

Interacts with SHANK1, SHANK2 and NOSTRIN. Interacts with SNX9. Interacts with SNX33 (via SH3 domain). Interacts with MYO1E (via SH3 domain). Interacts with CTTN and ACTN1 (By similarity). Interacts with MYOF and SH3BP4 (PubMed:17702744, PubMed:16325581). Interacts with PSTPIP1. Interacts with CTNND2 (By similarity). May interact with PIK3C3 (PubMed:18425118). May be a component of a complex composed of RAB5A (in GDP-bound form), DYN2 and PIK3C3 (PubMed:18425118).By similarity1 Publication2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AmphQ7TQF75EBI-642337,EBI-775139
CttnQ605982EBI-642337,EBI-397955
CTTN1Q014062EBI-642337,EBI-2530463From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199258. 7 interactions.
IntActiP39054. 18 interactions.
MINTiMINT-1605893.
STRINGi10090.ENSMUSP00000133564.

Structurei

3D structure databases

ProteinModelPortaliP39054.
SMRiP39054. Positions 6-739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 294267Dynamin-type GAdd
BLAST
Domaini519 – 625107PHPROSITE-ProRule annotationAdd
BLAST
Domaini653 – 74492GEDPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi747 – 866120Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 GED domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.
GeneTreeiENSGT00760000119213.
HOGENOMiHOG000161069.
HOVERGENiHBG107833.
InParanoidiP39054.
KOiK01528.
PhylomeDBiP39054.
TreeFamiTF300362.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR027188. DNM2.
IPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PTHR11566:SF23. PTHR11566:SF23. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P39054-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNRGMEELI PLVNKLQDAF SSIGQSCHLD LPQIAVVGGQ SAGKSSVLEN
60 70 80 90 100
FVGRDFLPRG SGIVTRRPLI LQLIFSKTEY AEFLHCKSKK FTDFDEVRQE
110 120 130 140 150
IEAETDRVTG TNKGISPVPI NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP
160 170 180 190 200
DIEYQIKDMI LQFISRESSL ILAVTPANMD LANSDALKLA KEVDPQGLRT
210 220 230 240 250
IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK DIEGKKDIRA
260 270 280 290 300
ALAAERKFFL SHPAYRHMAD RMGTPHLQKT LNQQLTNHIR ESLPTLRSKL
310 320 330 340 350
QSQLLSLEKE VEEYKNFRPD DPTRKTKALL QMVQQFGVDF EKRIEGSGDQ
360 370 380 390 400
VDTLELSGGA RINRIFHERF PFELVKMEFD EKDLRREISY AIKNIHGVRT
410 420 430 440 450
GLFTPDLAFE AIVKKQVVKL KEPCLKCVDL VIQELISTVR QCTSKLSSYP
460 470 480 490 500
RLREETERIV TTYIREREGR TKDQILLLID IEQSYINTNH EDFIGFANAQ
510 520 530 540 550
QRSTQLNKKR AIPNQGEILV IRRGWLTINN ISLMKGGSKE YWFVLTAESL
560 570 580 590 600
SWYKDEEEKE KKYMLPLDNL KIRDVEKGFM SNKHVFAIFN TEQRNVYKDL
610 620 630 640 650
RQIELACDSQ EDVDSWKASF LRAGVYPEKD QAENEDGAQE NTFSMDPQLE
660 670 680 690 700
RQVETIRNLV DSYVAIINKS IRDLMPKTIM HLMINNTKAF IHHELLAYLY
710 720 730 740 750
SSADQSSLME ESAEQAQRRD DMLRMYHALK EALNIIGDIS TSTVSTPVPP
760 770 780 790 800
PVDDTWLQNT SGHSPTPQRR PVSSVHPPGR PPAVRGPTPG PPLIPMPVGA
810 820 830 840 850
TSSFSAPPIP SRPGPQSVFA NNDPFSAPPQ IPSRPARIPP GIPPGVPSRR
860 870
APAAPSRPTI IRPAEPSLLD
Length:870
Mass (Da):98,145
Last modified:July 26, 2002 - v2
Checksum:iE80864AF94B8F778
GO
Isoform 2 (identifier: P39054-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     516-519: Missing.

Show »
Length:866
Mass (Da):97,733
Checksum:iCA0BEB6FA5E5028D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti297 – 2982RS → HG in AAA40523 (PubMed:9143510).Curated
Sequence conflicti848 – 87023SRRAP…PSLLD → RRPPPLAPARPFF in BAB23745 (PubMed:16141072).CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei516 – 5194Missing in isoform 2. 1 PublicationVSP_001326

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31398 mRNA. Translation: AAA40523.1.
AK005012 mRNA. Translation: BAB23745.1.
CCDSiCCDS57657.1. [P39054-1]
CCDS57659.1. [P39054-2]
RefSeqiNP_001240822.1. NM_001253893.1. [P39054-1]
NP_001240823.1. NM_001253894.1.
NP_031897.2. NM_007871.2. [P39054-2]
UniGeneiMm.433257.

Genome annotation databases

EnsembliENSMUST00000091087; ENSMUSP00000088616; ENSMUSG00000033335. [P39054-2]
ENSMUST00000172482; ENSMUSP00000133564; ENSMUSG00000033335. [P39054-1]
GeneIDi13430.
KEGGimmu:13430.
UCSCiuc009olk.2. mouse. [P39054-2]
uc029wxt.1. mouse. [P39054-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31398 mRNA. Translation: AAA40523.1.
AK005012 mRNA. Translation: BAB23745.1.
CCDSiCCDS57657.1. [P39054-1]
CCDS57659.1. [P39054-2]
RefSeqiNP_001240822.1. NM_001253893.1. [P39054-1]
NP_001240823.1. NM_001253894.1.
NP_031897.2. NM_007871.2. [P39054-2]
UniGeneiMm.433257.

3D structure databases

ProteinModelPortaliP39054.
SMRiP39054. Positions 6-739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199258. 7 interactions.
IntActiP39054. 18 interactions.
MINTiMINT-1605893.
STRINGi10090.ENSMUSP00000133564.

PTM databases

iPTMnetiP39054.
PhosphoSiteiP39054.

2D gel databases

REPRODUCTION-2DPAGEIPI00131445.

Proteomic databases

MaxQBiP39054.
PaxDbiP39054.
PeptideAtlasiP39054.
PRIDEiP39054.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000091087; ENSMUSP00000088616; ENSMUSG00000033335. [P39054-2]
ENSMUST00000172482; ENSMUSP00000133564; ENSMUSG00000033335. [P39054-1]
GeneIDi13430.
KEGGimmu:13430.
UCSCiuc009olk.2. mouse. [P39054-2]
uc029wxt.1. mouse. [P39054-1]

Organism-specific databases

CTDi1785.
MGIiMGI:109547. Dnm2.

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.
GeneTreeiENSGT00760000119213.
HOGENOMiHOG000161069.
HOVERGENiHBG107833.
InParanoidiP39054.
KOiK01528.
PhylomeDBiP39054.
TreeFamiTF300362.

Enzyme and pathway databases

BRENDAi3.6.5.5. 3474.
ReactomeiR-MMU-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-MMU-190873. Gap junction degradation.
R-MMU-196025. Formation of annular gap junctions.
R-MMU-203641. NOSTRIN mediated eNOS trafficking.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-437239. Recycling pathway of L1.

Miscellaneous databases

ChiTaRSiDnm2. mouse.
PROiP39054.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033335.
CleanExiMM_DNM2.
ExpressionAtlasiP39054. baseline and differential.
GenevisibleiP39054. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR027188. DNM2.
IPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PTHR11566:SF23. PTHR11566:SF23. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDYN2_MOUSE
AccessioniPrimary (citable) accession number: P39054
Secondary accession number(s): Q9DBE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 26, 2002
Last modified: September 7, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.