Reviewed, UniProtKB/Swiss-Prot P39054 (DYN2_MOUSE)

Names and origin

Protein names

Recommended name:
    Dynamin-2
    EC=3.6.5.5
Alternative name(s):
    Dynamin UDNM

Gene names

Name:	Dnm2
Synonyms:	Dyn2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	870 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
Catalytic activity	GTP + H2O = GDP + phosphate.
Subunit structure	Interacts with SHANK1, SHANK2 and NOSTRIN By similarity. Interacts with MYOF and SH3BP4. Ref.4 Ref.5
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density By similarity. Cell junction › synapse By similarity. Note: Microtubule-associated. Also found in the postsynaptic density of neuronal cells By similarity.
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell junction Cell membrane Cytoplasm Cytoskeleton Membrane Microtubule Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Motor protein
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro motor activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Grb2	Q60631	2	EBI-642353,EBI-1688

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P39054-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P39054-2)

The sequence of this isoform differs from the canonical sequence as follows:
     516-519: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 870	870	Dynamin-2		PRO_0000206571
Regions
	Domain	519 – 625	107	PH
	Domain	653 – 744	92	GED
	Nucleotide binding	38 – 45	8	GTP By similarity
	Nucleotide binding	136 – 140	5	GTP By similarity
	Nucleotide binding	205 – 208	4	GTP By similarity
	Compositional bias	747 – 866	120	Pro-rich
Amino acid modifications
	Modified residue	298	1	Phosphoserine Ref.6
	Modified residue	302	1	Phosphoserine Ref.6
	Modified residue	449	1	Phosphotyrosine By similarity
	Modified residue	597	1	Phosphotyrosine Ref.6
	Modified residue	598	1	N6-acetyllysine By similarity
	Modified residue	766	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	516 – 519	4	Missing in isoform 2.		VSP_001326
Experimental info
	Sequence conflict	297 – 298	2	RS → HG in AAA40523. Ref.1
	Sequence conflict	848 – 870	23	SRRAP…PSLLD → RRPPPLAPARPFF in BAB23745. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 26, 2002. Version 2. Checksum: E80864AF94B8F778	FASTA	870	98,145
	10 20 30 40 50 60 MGNRGMEELI PLVNKLQDAF SSIGQSCHLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLI LQLIFSKTEY AEFLHCKSKK FTDFDEVRQE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIKDMI LQFISRESSL ILAVTPANMD 190 200 210 220 230 240 LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIEGKKDIRA ALAAERKFFL SHPAYRHMAD RMGTPHLQKT LNQQLTNHIR ESLPTLRSKL 310 320 330 340 350 360 QSQLLSLEKE VEEYKNFRPD DPTRKTKALL QMVQQFGVDF EKRIEGSGDQ VDTLELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKDLRREISY AIKNIHGVRT GLFTPDLAFE AIVKKQVVKL 430 440 450 460 470 480 KEPCLKCVDL VIQELISTVR QCTSKLSSYP RLREETERIV TTYIREREGR TKDQILLLID 490 500 510 520 530 540 IEQSYINTNH EDFIGFANAQ QRSTQLNKKR AIPNQGEILV IRRGWLTINN ISLMKGGSKE 550 560 570 580 590 600 YWFVLTAESL SWYKDEEEKE KKYMLPLDNL KIRDVEKGFM SNKHVFAIFN TEQRNVYKDL 610 620 630 640 650 660 RQIELACDSQ EDVDSWKASF LRAGVYPEKD QAENEDGAQE NTFSMDPQLE RQVETIRNLV 670 680 690 700 710 720 DSYVAIINKS IRDLMPKTIM HLMINNTKAF IHHELLAYLY SSADQSSLME ESAEQAQRRD 730 740 750 760 770 780 DMLRMYHALK EALNIIGDIS TSTVSTPVPP PVDDTWLQNT SGHSPTPQRR PVSSVHPPGR 790 800 810 820 830 840 PPAVRGPTPG PPLIPMPVGA TSSFSAPPIP SRPGPQSVFA NNDPFSAPPQ IPSRPARIPP 850 860 870 GIPPGVPSRR APAAPSRPTI IRPAEPSLLD
	Isoform 2. Checksum: CA0BEB6FA5E5028D	FASTA	866	97,733
	10 20 30 40 50 60 MGNRGMEELI PLVNKLQDAF SSIGQSCHLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLI LQLIFSKTEY AEFLHCKSKK FTDFDEVRQE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIKDMI LQFISRESSL ILAVTPANMD 190 200 210 220 230 240 LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIEGKKDIRA ALAAERKFFL SHPAYRHMAD RMGTPHLQKT LNQQLTNHIR ESLPTLRSKL 310 320 330 340 350 360 QSQLLSLEKE VEEYKNFRPD DPTRKTKALL QMVQQFGVDF EKRIEGSGDQ VDTLELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKDLRREISY AIKNIHGVRT GLFTPDLAFE AIVKKQVVKL 430 440 450 460 470 480 KEPCLKCVDL VIQELISTVR QCTSKLSSYP RLREETERIV TTYIREREGR TKDQILLLID 490 500 510 520 530 540 IEQSYINTNH EDFIGFANAQ QRSTQLNKKR AIPNQVIRRG WLTINNISLM KGGSKEYWFV 550 560 570 580 590 600 LTAESLSWYK DEEEKEKKYM LPLDNLKIRD VEKGFMSNKH VFAIFNTEQR NVYKDLRQIE 610 620 630 640 650 660 LACDSQEDVD SWKASFLRAG VYPEKDQAEN EDGAQENTFS MDPQLERQVE TIRNLVDSYV 670 680 690 700 710 720 AIINKSIRDL MPKTIMHLMI NNTKAFIHHE LLAYLYSSAD QSSLMEESAE QAQRRDDMLR 730 740 750 760 770 780 MYHALKEALN IIGDISTSTV STPVPPPVDD TWLQNTSGHS PTPQRRPVSS VHPPGRPPAV 790 800 810 820 830 840 RGPTPGPPLI PMPVGATSSF SAPPIPSRPG PQSVFANNDP FSAPPQIPSR PARIPPGIPP 850 860 GVPSRRAPAA PSRPTIIRPA EPSLLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dynamin genes Dnm1 and Dnm2 are located on proximal mouse chromosomes 2 and 9, respectively." Klocke R., Augustin A., Ronsiek M., Stief A., van der Putten H., Jockusch H. Genomics 41:290-292(1997) [PubMed: 9143510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: NIH Swiss.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Liver.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 45-54; 91-107; 114-142; 207-217; 230-237; 300-309; 370-376 AND 678-688, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[4]	"TTP specifically regulates the internalization of the transferrin receptor." Tosoni D., Puri C., Confalonieri S., Salcini A.E., De Camilli P., Tacchetti C., Di Fiore P.P. Cell 123:875-888(2005) [PubMed: 16325581] [Abstract] Cited for: INTERACTION WITH SH3BP4.
[5]	"Myoferlin regulates vascular endothelial growth factor receptor-2 stability and function." Bernatchez P.N., Acevedo L., Fernandez-Hernando C., Murata T., Chalouni C., Kim J., Erdjument-Bromage H., Shah V., Gratton J.-P., McNally E.M., Tempst P., Sessa W.C. J. Biol. Chem. 282:30745-30753(2007) [PubMed: 17702744] [Abstract] Cited for: INTERACTION WITH MYOF.
[6]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-298; SER-302 AND TYR-597, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L31398 mRNA. Translation: AAA40523.1. AK005012 mRNA. Translation: BAB23745.1.
IPI	IPI00131445. IPI00314883.
UniGene	Mm.433257
3D structure databases
SMR	P39054. Positions 6-304, 520-625.
ModBase	Search...
Protein-protein interaction databases
IntAct	P39054. 4 interactions.
STRING	P39054.
PTM databases
PhosphoSite	P39054.
Proteomic databases
PRIDE	P39054.
Genome annotation databases
Ensembl	ENSMUST00000072362; ENSMUSP00000072199; ENSMUSG00000033335; Mus musculus. [Genome view] ENSMUST00000091087; ENSMUSP00000088616; ENSMUSG00000033335; Mus musculus. [Genome view]
UCSC	uc009olk.1. mouse.
Organism-specific databases
MGI	MGI:109547. Dnm2.
Phylogenomic databases
eggNOG	roNOG04822.
HOGENOM	HBG434086.
HOVERGEN	P39054.
InParanoid	P39054.
OMA	NNDPFSA.
PhylomeDB	P39054.
Enzyme and pathway databases
BRENDA	3.6.5.5. 244.
Gene expression databases
ArrayExpress	P39054.
Bgee	P39054.
CleanEx	MM_DNM2.
Genevestigator	P39054.
GermOnline	ENSMUSG00000033335. Mus musculus.
Family and domain databases
InterPro	IPR000375. Dynamin_central. IPR001401. Dynamin_GTPase. IPR019762. Dynamin_GTPase_CS. IPR003130. GED. IPR020850. GTPase_effector_domain_GED. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF01031. Dynamin_M. 1 hit. PF00350. Dynamin_N. 1 hit. PF02212. GED. 1 hit. PF00169. PH. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
SMART	SM00053. DYNc. 1 hit. SM00302. GED. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS00410. DYNAMIN. 1 hit. PS51388. GED. 1 hit. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

DYN2_MOUSE

Accession

Primary (citable) accession number: P39054
Secondary accession number(s): Q9DBE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 26, 2002
Last modified:	January 19, 2010
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)