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P39054

- DYN2_MOUSE

UniProt

P39054 - DYN2_MOUSE

Protein

Dynamin-2

Gene

Dnm2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (26 Jul 2002)
      Previous versions | rss
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    Functioni

    Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis.1 Publication

    Catalytic activityi

    GTP + H2O = GDP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi38 – 458GTPBy similarity
    Nucleotide bindingi136 – 1405GTPBy similarity
    Nucleotide bindingi205 – 2084GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. receptor internalization Source: Ensembl
    2. transferrin transport Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Motor protein

    Keywords - Biological processi

    Endocytosis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196550. MHC class II antigen presentation.
    REACT_202778. Retrograde neurotrophin signalling.
    REACT_209040. Gap junction degradation.
    REACT_215995. NOSTRIN mediated eNOS trafficking.
    REACT_220352. Formation of annular gap junctions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dynamin-2 (EC:3.6.5.5)
    Alternative name(s):
    Dynamin UDNM
    Gene namesi
    Name:Dnm2
    Synonyms:Dyn2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:109547. Dnm2.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity. Cell junctionsynapse By similarity. Midbody By similarity
    Note: Microtubule-associated. Also found in the postsynaptic density of neuronal cells By similarity.By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytoplasm Source: UniProtKB-SubCell
    3. microtubule Source: UniProtKB-KW
    4. midbody Source: UniProtKB-SubCell
    5. postsynaptic density Source: UniProtKB-SubCell
    6. postsynaptic membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Disruption phenotypei

    Exhibits growth and cytokinesis defects.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 870870Dynamin-2PRO_0000206571Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei299 – 2991N6-acetyllysine1 Publication
    Modified residuei598 – 5981N6-acetyllysineBy similarity
    Modified residuei764 – 7641Phosphoserine; by CDK1By similarity

    Post-translational modificationi

    Phosphorylation at Ser-764 by CDK1 is greatly increased upon mitotic entry. It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis. Dephosphorylated by Calcineurin/PP2 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP39054.
    PaxDbiP39054.
    PRIDEiP39054.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00131445.

    PTM databases

    PhosphoSiteiP39054.

    Expressioni

    Tissue specificityi

    Expressed in most tissues during embryonic development, including the peripheral nervous system although no expression is evident in skeletal muscle or heart.1 Publication

    Gene expression databases

    ArrayExpressiP39054.
    BgeeiP39054.
    CleanExiMM_DNM2.
    GenevestigatoriP39054.

    Interactioni

    Subunit structurei

    Interacts with SHANK1, SHANK2 and NOSTRIN By similarity. Interacts with SNX9. Interacts with SNX33 (via SH3 domain). Interacts with MYO1E (via SH3 domain) By similarity. Interacts with MYOF and SH3BP4. Interacts with PSTPIP1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CttnQ605982EBI-642337,EBI-397955
    CTTN1Q014062EBI-642337,EBI-2530463From a different organism.

    Protein-protein interaction databases

    IntActiP39054. 15 interactions.
    MINTiMINT-1605893.

    Structurei

    3D structure databases

    ProteinModelPortaliP39054.
    SMRiP39054. Positions 6-740.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 294267Dynamin-type GAdd
    BLAST
    Domaini519 – 625107PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini653 – 74492GEDPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi747 – 866120Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 GED domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0699.
    GeneTreeiENSGT00740000115253.
    HOGENOMiHOG000161069.
    HOVERGENiHBG107833.
    InParanoidiP39054.
    KOiK01528.
    PhylomeDBiP39054.
    TreeFamiTF300362.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR027188. DNM2.
    IPR000375. Dynamin_central.
    IPR001401. Dynamin_GTPase.
    IPR019762. Dynamin_GTPase_CS.
    IPR022812. Dynamin_SF.
    IPR003130. GED.
    IPR020850. GTPase_effector_domain_GED.
    IPR027417. P-loop_NTPase.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view]
    PANTHERiPTHR11566. PTHR11566. 1 hit.
    PTHR11566:SF23. PTHR11566:SF23. 1 hit.
    PfamiPF01031. Dynamin_M. 1 hit.
    PF00350. Dynamin_N. 1 hit.
    PF02212. GED. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view]
    PRINTSiPR00195. DYNAMIN.
    SMARTiSM00053. DYNc. 1 hit.
    SM00302. GED. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    PS51388. GED. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P39054-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGNRGMEELI PLVNKLQDAF SSIGQSCHLD LPQIAVVGGQ SAGKSSVLEN    50
    FVGRDFLPRG SGIVTRRPLI LQLIFSKTEY AEFLHCKSKK FTDFDEVRQE 100
    IEAETDRVTG TNKGISPVPI NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP 150
    DIEYQIKDMI LQFISRESSL ILAVTPANMD LANSDALKLA KEVDPQGLRT 200
    IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK DIEGKKDIRA 250
    ALAAERKFFL SHPAYRHMAD RMGTPHLQKT LNQQLTNHIR ESLPTLRSKL 300
    QSQLLSLEKE VEEYKNFRPD DPTRKTKALL QMVQQFGVDF EKRIEGSGDQ 350
    VDTLELSGGA RINRIFHERF PFELVKMEFD EKDLRREISY AIKNIHGVRT 400
    GLFTPDLAFE AIVKKQVVKL KEPCLKCVDL VIQELISTVR QCTSKLSSYP 450
    RLREETERIV TTYIREREGR TKDQILLLID IEQSYINTNH EDFIGFANAQ 500
    QRSTQLNKKR AIPNQGEILV IRRGWLTINN ISLMKGGSKE YWFVLTAESL 550
    SWYKDEEEKE KKYMLPLDNL KIRDVEKGFM SNKHVFAIFN TEQRNVYKDL 600
    RQIELACDSQ EDVDSWKASF LRAGVYPEKD QAENEDGAQE NTFSMDPQLE 650
    RQVETIRNLV DSYVAIINKS IRDLMPKTIM HLMINNTKAF IHHELLAYLY 700
    SSADQSSLME ESAEQAQRRD DMLRMYHALK EALNIIGDIS TSTVSTPVPP 750
    PVDDTWLQNT SGHSPTPQRR PVSSVHPPGR PPAVRGPTPG PPLIPMPVGA 800
    TSSFSAPPIP SRPGPQSVFA NNDPFSAPPQ IPSRPARIPP GIPPGVPSRR 850
    APAAPSRPTI IRPAEPSLLD 870
    Length:870
    Mass (Da):98,145
    Last modified:July 26, 2002 - v2
    Checksum:iE80864AF94B8F778
    GO
    Isoform 2 (identifier: P39054-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         516-519: Missing.

    Show »
    Length:866
    Mass (Da):97,733
    Checksum:iCA0BEB6FA5E5028D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti297 – 2982RS → HG in AAA40523. (PubMed:9143510)Curated
    Sequence conflicti848 – 87023SRRAP…PSLLD → RRPPPLAPARPFF in BAB23745. (PubMed:16141072)CuratedAdd
    BLAST

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei516 – 5194Missing in isoform 2. 1 PublicationVSP_001326

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L31398 mRNA. Translation: AAA40523.1.
    AK005012 mRNA. Translation: BAB23745.1.
    CCDSiCCDS57657.1. [P39054-1]
    CCDS57659.1. [P39054-2]
    RefSeqiNP_001240822.1. NM_001253893.1. [P39054-1]
    NP_001240823.1. NM_001253894.1.
    NP_031897.2. NM_007871.2. [P39054-2]
    UniGeneiMm.433257.

    Genome annotation databases

    EnsembliENSMUST00000091087; ENSMUSP00000088616; ENSMUSG00000033335. [P39054-2]
    ENSMUST00000172482; ENSMUSP00000133564; ENSMUSG00000033335. [P39054-1]
    GeneIDi13430.
    KEGGimmu:13430.
    UCSCiuc009olk.2. mouse. [P39054-2]
    uc029wxt.1. mouse. [P39054-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L31398 mRNA. Translation: AAA40523.1 .
    AK005012 mRNA. Translation: BAB23745.1 .
    CCDSi CCDS57657.1. [P39054-1 ]
    CCDS57659.1. [P39054-2 ]
    RefSeqi NP_001240822.1. NM_001253893.1. [P39054-1 ]
    NP_001240823.1. NM_001253894.1.
    NP_031897.2. NM_007871.2. [P39054-2 ]
    UniGenei Mm.433257.

    3D structure databases

    ProteinModelPortali P39054.
    SMRi P39054. Positions 6-740.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P39054. 15 interactions.
    MINTi MINT-1605893.

    PTM databases

    PhosphoSitei P39054.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00131445.

    Proteomic databases

    MaxQBi P39054.
    PaxDbi P39054.
    PRIDEi P39054.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000091087 ; ENSMUSP00000088616 ; ENSMUSG00000033335 . [P39054-2 ]
    ENSMUST00000172482 ; ENSMUSP00000133564 ; ENSMUSG00000033335 . [P39054-1 ]
    GeneIDi 13430.
    KEGGi mmu:13430.
    UCSCi uc009olk.2. mouse. [P39054-2 ]
    uc029wxt.1. mouse. [P39054-1 ]

    Organism-specific databases

    CTDi 1785.
    MGIi MGI:109547. Dnm2.

    Phylogenomic databases

    eggNOGi COG0699.
    GeneTreei ENSGT00740000115253.
    HOGENOMi HOG000161069.
    HOVERGENi HBG107833.
    InParanoidi P39054.
    KOi K01528.
    PhylomeDBi P39054.
    TreeFami TF300362.

    Enzyme and pathway databases

    Reactomei REACT_196550. MHC class II antigen presentation.
    REACT_202778. Retrograde neurotrophin signalling.
    REACT_209040. Gap junction degradation.
    REACT_215995. NOSTRIN mediated eNOS trafficking.
    REACT_220352. Formation of annular gap junctions.

    Miscellaneous databases

    ChiTaRSi DNM2. mouse.
    NextBioi 283855.
    PROi P39054.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P39054.
    Bgeei P39054.
    CleanExi MM_DNM2.
    Genevestigatori P39054.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR027188. DNM2.
    IPR000375. Dynamin_central.
    IPR001401. Dynamin_GTPase.
    IPR019762. Dynamin_GTPase_CS.
    IPR022812. Dynamin_SF.
    IPR003130. GED.
    IPR020850. GTPase_effector_domain_GED.
    IPR027417. P-loop_NTPase.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view ]
    PANTHERi PTHR11566. PTHR11566. 1 hit.
    PTHR11566:SF23. PTHR11566:SF23. 1 hit.
    Pfami PF01031. Dynamin_M. 1 hit.
    PF00350. Dynamin_N. 1 hit.
    PF02212. GED. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view ]
    PRINTSi PR00195. DYNAMIN.
    SMARTi SM00053. DYNc. 1 hit.
    SM00302. GED. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00410. G_DYNAMIN_1. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    PS51388. GED. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dynamin genes Dnm1 and Dnm2 are located on proximal mouse chromosomes 2 and 9, respectively."
      Klocke R., Augustin A., Ronsiek M., Stief A., van der Putten H., Jockusch H.
      Genomics 41:290-292(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: NIH Swiss.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Liver.
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 45-54; 91-107; 114-142; 207-217; 230-237; 300-309; 370-376 AND 678-688, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    4. "TTP specifically regulates the internalization of the transferrin receptor."
      Tosoni D., Puri C., Confalonieri S., Salcini A.E., De Camilli P., Tacchetti C., Di Fiore P.P.
      Cell 123:875-888(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SH3BP4.
    5. Cited for: INTERACTION WITH MYOF.
    6. "Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cells."
      Liu Y.W., Surka M.C., Schroeter T., Lukiyanchuk V., Schmid S.L.
      Mol. Biol. Cell 19:5347-5359(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    7. Cited for: TISSUE SPECIFICITY.
    8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiDYN2_MOUSE
    AccessioniPrimary (citable) accession number: P39054
    Secondary accession number(s): Q9DBE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 26, 2002
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3