Reviewed, UniProtKB/Swiss-Prot P39052 (DYN2_RAT)

Names and origin

Protein names

Recommended name:
    Dynamin-2
    EC=3.6.5.5

Gene names

Name:	Dnm2
Synonyms:	Dyn2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	870 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
Catalytic activity	GTP + H2O = GDP + phosphate.
Subunit structure	Interacts with MYOF, SHANK1, SHANK2 and NOSTRIN By similarity. Interacts with SH3BP4.
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density By similarity. Cell junction › synapse By similarity. Note: Microtubule-associated. Also found in the postsynaptic density of neuronal cells By similarity. Isoform 2: Cytoplasmic vesicle › clathrin-coated vesicle. Membrane › clathrin-coated pit. Ref.3 Isoform 4: Cell membrane. Ref.3
Tissue specificity	Ubiquitously expressed. Brain expression is restricted to glial cells and fibroblasts. Highest levels in the testis. Ref.3
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell junction Cell membrane Coated pit Cytoplasm Cytoplasmic vesicle Cytoskeleton Membrane Microtubule Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Motor protein
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	receptor-mediated endocytosis Inferred from direct assay. Source: RGD
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW clathrin-coated endocytic vesicle Inferred from direct assay. Source: RGD microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: RGD postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	GTP binding Traceable author statement. Source: RGD GTPase activity Traceable author statement. Source: RGD motor activity Inferred from electronic annotation. Source: UniProtKB-KW protein complex binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P39052-1)

Also known as: ba; IIBA;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P39052-2)

Also known as: aa;

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEAIVKKQ...LISTVRQCTS → MAFEAIVKKQ...LATVIKKCAE

Note: Sequestered to clathrin-coated pits and vesicles at the plasma membrane and the Golgi apparatus.

Isoform 3 (identifier: P39052-3)

Also known as: bb; IIC;

The sequence of this isoform differs from the canonical sequence as follows:
     516-519: Missing.

Isoform 4 (identifier: P39052-4)

Also known as: ab;

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEAIVKKQ...LISTVRQCTS → MAFEAIVKKQ...LATVIKKCAE
     516-519: Missing.

Note: Localized to numerous punctate spots on the plasma membrane.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 870	870	Dynamin-2		PRO_0000206572
Regions
	Domain	519 – 625	107	PH
	Domain	653 – 744	92	GED
	Nucleotide binding	38 – 45	8	GTP By similarity
	Nucleotide binding	136 – 140	5	GTP By similarity
	Nucleotide binding	205 – 208	4	GTP By similarity
	Compositional bias	747 – 866	120	Pro-rich
Amino acid modifications
	Modified residue	298	1	Phosphoserine By similarity
	Modified residue	302	1	Phosphoserine By similarity
	Modified residue	449	1	Phosphotyrosine By similarity
	Modified residue	597	1	Phosphotyrosine By similarity
	Modified residue	598	1	N6-acetyllysine By similarity
	Modified residue	766	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	407 – 444	38	LAFEA…RQCTS → MAFEAIVKKQLVKLKEPSLK CVDLVVSELATVIKKCAE in isoform 2 and isoform 4.		VSP_001327
	Alternative sequence	516 – 519	4	Missing in isoform 3 and isoform 4.		VSP_001328
Experimental info
	Sequence conflict	298	1	S → T in AAA16746. Ref.2
	Sequence conflict	389	1	S → T in AAA16746. Ref.2
	Sequence conflict	487	1	N → K in AAA16746. Ref.2
	Sequence conflict	637	1	G → E in AAA16746. Ref.2
	Sequence conflict	719	1	Missing in AAA16746. Ref.2
	Sequence conflict	786 – 791	6	GPTPGP → PHTGA in AAA16746. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ba) (IIBA) [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 3802DBAFA3ABBE98	FASTA	870	98,230
	10 20 30 40 50 60 MGNRGMEELI PLVNKLQDAF SSIGQSCHLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLI LQLIFSKTEY AEFLHCKSKK FTDFDEVRQE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIKDMI LQFISRESSL ILAVTPANMD 190 200 210 220 230 240 LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIEGRKDIRA ALAAERKFFL SHPAYRHMAD RMGTPHLQKT LNQQLTNHIR ESLPTLRSKL 310 320 330 340 350 360 QSQLLSLEKE VEEYKNFRPD DPTRKTKALL QMVQQFGVDF EKRIEGSGDQ VDTLELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKDLRREISY AIKNIHGVRT GLFTPDLAFE AIVKKQVVKL 430 440 450 460 470 480 KEPCLKCVDL VIQELISTVR QCTSKLSSYP RLREETERIV TTYIREREGR TKDQILLLID 490 500 510 520 530 540 IEQSYINTNH EDFIGFANAQ QRSTQLNKKR AIPNQGEILV IRRGWLTINN ISLMKGGSKE 550 560 570 580 590 600 YWFVLTAESL SWYKDEEEKE KKYMLPLDNL KIRDVEKGFM SNKHVFAIFN TEQRNVYKDL 610 620 630 640 650 660 RQIELACDSQ EDVDSWKASF LRAGVYPEKD QAENEDGAQE NTFSMDPQLE RQVETIRNLV 670 680 690 700 710 720 DSYVAIINKS IRDLMPKTIM HLMINNTKAF IHHELLAYLY SSADQSSLME ESAEQAQRRD 730 740 750 760 770 780 DMLRMYHALK EALNIIGDIS TSTVSTPVPP PVDDTWLQNT SSHSPTPQRR PVSSVHPPGR 790 800 810 820 830 840 PPAVRGPTPG PPLIPMPVGA TSSFSAPPIP SRPGPQNVFA NNDPFSAPPQ IPSRPARIPP 850 860 870 GIPPGVPSRR APAAPSRPTI IRPAEPSLLD
	Isoform 2 (aa). Checksum: F06960A8E93126A6	FASTA	870	98,159
	10 20 30 40 50 60 MGNRGMEELI PLVNKLQDAF SSIGQSCHLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLI LQLIFSKTEY AEFLHCKSKK FTDFDEVRQE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIKDMI LQFISRESSL ILAVTPANMD 190 200 210 220 230 240 LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIEGRKDIRA ALAAERKFFL SHPAYRHMAD RMGTPHLQKT LNQQLTNHIR ESLPTLRSKL 310 320 330 340 350 360 QSQLLSLEKE VEEYKNFRPD DPTRKTKALL QMVQQFGVDF EKRIEGSGDQ VDTLELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKDLRREISY AIKNIHGVRT GLFTPDMAFE AIVKKQLVKL 430 440 450 460 470 480 KEPSLKCVDL VVSELATVIK KCAEKLSSYP RLREETERIV TTYIREREGR TKDQILLLID 490 500 510 520 530 540 IEQSYINTNH EDFIGFANAQ QRSTQLNKKR AIPNQGEILV IRRGWLTINN ISLMKGGSKE 550 560 570 580 590 600 YWFVLTAESL SWYKDEEEKE KKYMLPLDNL KIRDVEKGFM SNKHVFAIFN TEQRNVYKDL 610 620 630 640 650 660 RQIELACDSQ EDVDSWKASF LRAGVYPEKD QAENEDGAQE NTFSMDPQLE RQVETIRNLV 670 680 690 700 710 720 DSYVAIINKS IRDLMPKTIM HLMINNTKAF IHHELLAYLY SSADQSSLME ESAEQAQRRD 730 740 750 760 770 780 DMLRMYHALK EALNIIGDIS TSTVSTPVPP PVDDTWLQNT SSHSPTPQRR PVSSVHPPGR 790 800 810 820 830 840 PPAVRGPTPG PPLIPMPVGA TSSFSAPPIP SRPGPQNVFA NNDPFSAPPQ IPSRPARIPP 850 860 870 GIPPGVPSRR APAAPSRPTI IRPAEPSLLD
	Isoform 3 (bb) (IIC). Checksum: 0B3D9DB3C7A14827	FASTA	866	97,818
	10 20 30 40 50 60 MGNRGMEELI PLVNKLQDAF SSIGQSCHLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLI LQLIFSKTEY AEFLHCKSKK FTDFDEVRQE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIKDMI LQFISRESSL ILAVTPANMD 190 200 210 220 230 240 LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIEGRKDIRA ALAAERKFFL SHPAYRHMAD RMGTPHLQKT LNQQLTNHIR ESLPTLRSKL 310 320 330 340 350 360 QSQLLSLEKE VEEYKNFRPD DPTRKTKALL QMVQQFGVDF EKRIEGSGDQ VDTLELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKDLRREISY AIKNIHGVRT GLFTPDLAFE AIVKKQVVKL 430 440 450 460 470 480 KEPCLKCVDL VIQELISTVR QCTSKLSSYP RLREETERIV TTYIREREGR TKDQILLLID 490 500 510 520 530 540 IEQSYINTNH EDFIGFANAQ QRSTQLNKKR AIPNQVIRRG WLTINNISLM KGGSKEYWFV 550 560 570 580 590 600 LTAESLSWYK DEEEKEKKYM LPLDNLKIRD VEKGFMSNKH VFAIFNTEQR NVYKDLRQIE 610 620 630 640 650 660 LACDSQEDVD SWKASFLRAG VYPEKDQAEN EDGAQENTFS MDPQLERQVE TIRNLVDSYV 670 680 690 700 710 720 AIINKSIRDL MPKTIMHLMI NNTKAFIHHE LLAYLYSSAD QSSLMEESAE QAQRRDDMLR 730 740 750 760 770 780 MYHALKEALN IIGDISTSTV STPVPPPVDD TWLQNTSSHS PTPQRRPVSS VHPPGRPPAV 790 800 810 820 830 840 RGPTPGPPLI PMPVGATSSF SAPPIPSRPG PQNVFANNDP FSAPPQIPSR PARIPPGIPP 850 860 GVPSRRAPAA PSRPTIIRPA EPSLLD
	Isoform 4 (ab). Checksum: F1A7058D5B951106	FASTA	866	97,747
	10 20 30 40 50 60 MGNRGMEELI PLVNKLQDAF SSIGQSCHLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLI LQLIFSKTEY AEFLHCKSKK FTDFDEVRQE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIKDMI LQFISRESSL ILAVTPANMD 190 200 210 220 230 240 LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIEGRKDIRA ALAAERKFFL SHPAYRHMAD RMGTPHLQKT LNQQLTNHIR ESLPTLRSKL 310 320 330 340 350 360 QSQLLSLEKE VEEYKNFRPD DPTRKTKALL QMVQQFGVDF EKRIEGSGDQ VDTLELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKDLRREISY AIKNIHGVRT GLFTPDMAFE AIVKKQLVKL 430 440 450 460 470 480 KEPSLKCVDL VVSELATVIK KCAEKLSSYP RLREETERIV TTYIREREGR TKDQILLLID 490 500 510 520 530 540 IEQSYINTNH EDFIGFANAQ QRSTQLNKKR AIPNQVIRRG WLTINNISLM KGGSKEYWFV 550 560 570 580 590 600 LTAESLSWYK DEEEKEKKYM LPLDNLKIRD VEKGFMSNKH VFAIFNTEQR NVYKDLRQIE 610 620 630 640 650 660 LACDSQEDVD SWKASFLRAG VYPEKDQAEN EDGAQENTFS MDPQLERQVE TIRNLVDSYV 670 680 690 700 710 720 AIINKSIRDL MPKTIMHLMI NNTKAFIHHE LLAYLYSSAD QSSLMEESAE QAQRRDDMLR 730 740 750 760 770 780 MYHALKEALN IIGDISTSTV STPVPPPVDD TWLQNTSSHS PTPQRRPVSS VHPPGRPPAV 790 800 810 820 830 840 RGPTPGPPLI PMPVGATSSF SAPPIPSRPG PQNVFANNDP FSAPPQIPSR PARIPPGIPP 850 860 GVPSRRAPAA PSRPTIIRPA EPSLLD

References

[1]	"Differential expression and regulation of multiple dynamins." Sontag J.-M., Fykse E.M., Ushkaryov Y., Liu J.-P., Robinson P.J., Suedhof T.C. J. Biol. Chem. 269:4547-4554(1994) [PubMed: 8308025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1-4). Tissue: Brain.
[2]	"Identification of dynamin 2, an isoform ubiquitously expressed in rat tissues." Cook T.A., Urrutia R., McNiven M.A. Proc. Natl. Acad. Sci. U.S.A. 91:644-648(1994) [PubMed: 8290576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Strain: Sprague-Dawley.
[3]	"Differential distribution of dynamin isoforms in mammalian cells." Cao H., Garcia F., McNiven M.A. Mol. Biol. Cell 9:2595-2609(1998) [PubMed: 9725914] [Abstract] Cited for: ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]	"TTP specifically regulates the internalization of the transferrin receptor." Tosoni D., Puri C., Confalonieri S., Salcini A.E., De Camilli P., Tacchetti C., Di Fiore P.P. Cell 123:875-888(2005) [PubMed: 16325581] [Abstract] Cited for: INTERACTION WITH SH3BP4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L25605 mRNA. Translation: AAA19736.1. L24562 mRNA. Translation: AAA16746.1.
IPI	IPI00210319. IPI00389749. IPI00389750. IPI00896218.
PIR	A36878. A53165. B53165.
RefSeq	NP_037331.1.
UniGene	Rn.11231
3D structure databases
HSSP	HSSP built from PDB template 2DYN based on UniProtKB Q05193.
SMR	P39052. Positions 6-304, 518-629.
ModBase	Search...
Protein-protein interaction databases
STRING	P39052.
PTM databases
PhosphoSite	P39052.
Proteomic databases
PRIDE	P39052.
Genome annotation databases
Ensembl	ENSRNOT00000010362; ENSRNOP00000010362; ENSRNOG00000007649; Rattus norvegicus. [Genome view] ENSRNOT00000010947; ENSRNOP00000010948; ENSRNOG00000007649; Rattus norvegicus. [Genome view] ENSRNOT00000052366; ENSRNOP00000046629; ENSRNOG00000007649; Rattus norvegicus. [Genome view]
GeneID	25751.
KEGG	rno:25751.
UCSC	NM_013199. rat.
Organism-specific databases
CTD	25751.
RGD	2513. Dnm2.
Phylogenomic databases
HOVERGEN	P39052.
Enzyme and pathway databases
BRENDA	3.6.5.5. 248.
Gene expression databases
ArrayExpress	P39052.
Genevestigator	P39052.
GermOnline	ENSRNOG00000007649. Rattus norvegicus.
Family and domain databases
InterPro	IPR000375. Dynamin_central. IPR001401. Dynamin_GTPase. IPR019762. Dynamin_GTPase_CS. IPR003130. GED. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF01031. Dynamin_M. 1 hit. PF00350. Dynamin_N. 1 hit. PF02212. GED. 1 hit. PF00169. PH. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
SMART	SM00053. DYNc. 1 hit. SM00302. GED. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS00410. DYNAMIN. 1 hit. PS51388. GED. 1 hit. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	607943.

Entry information

Entry name

DYN2_RAT

Accession

Primary (citable) accession number: P39052

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)