Reviewed, UniProtKB/Swiss-Prot P39049 (ALXM_PHOS4)

Names and origin

Protein names

Recommended name:
    Alginate lyase
    EC=4.2.2.3
Alternative name(s):
    Poly(Beta-D-mannuronate) lyase
    Poly(mana) alginate lyase

Gene names

Name:

alxM

Organism

Photobacterium sp. (strain ATCC 433367)

Taxonomic identifier

39004 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends.
Sequence similarities	Belongs to the polysaccharide lyase 6 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.1
	Chain	21 – 285	265	Alginate lyase		PRO_0000024923

Sequences

	Sequence		Length	Mass (Da)
	P39049-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9F2918C4D8A87812	FASTA	285	32,129
	10 20 30 40 50 60 MIKSNLVISS LAIVSSMSYA GVEFSNPSGQ LGEPANYTQF ANILSASELQ ISDPNGKKGN 70 80 90 100 110 120 KEYFALDNDF TGIVNDNFYV DKQSQALVFK MANDHLRNEL RVQKNFRTDL PDHFYTLYAN 130 140 150 160 170 180 VEILHPLQSM ANSTSKQNEI TFLQVHNKGL DDQGTHNVPH PLLRVVWKEN NQGVKGHFWA 190 200 210 220 230 240 ITKNNAVICK GSFGKKNKDK EMCRADVAYS KIDLGPAPTD KGTDFTITVG NKTLAIDVNG 250 260 270 280 QRKVEKNIDY WRHLLSYFKA GVYNQFTQGE SEAHFNQLRY QVNTP

References

[1]

"Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate." Malissard M., Duez C., Guinand M., Vacheron M.-J., Michel G., Marty N., Joris B., Thamm I., Ghuysen J.-M. FEMS Microbiol. Lett. 110:101-106(1993) [PubMed: 8319887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 21-27.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70036 Genomic DNA. Translation: CAA49630.1.
PIR	S31708.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL7. Polysaccharide Lyase Family 7.
Enzyme and pathway databases
BRENDA	4.2.2.3. 39800.
Family and domain databases
InterPro	IPR014895. Alginate_lyase_2. [Graphical view]
Pfam	PF08787. Alginate_lyase2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALXM_PHOS4

Accession

Primary (citable) accession number: P39049

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 14, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)