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P39046

- MUR2_ENTHA

UniProt

P39046 - MUR2_ENTHA

Protein

Muramidase-2

Gene

EHR_05900

Organism
Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    May work in concert with and potentiate the processive hydrolytic action of muramidase-1, which requires binding of the enzyme to non-reducing ends of glycan chains. Hydrolysis in the midst of glycan chains would increase the number of binding sites for muramidase-1. May function in facilitating septum formation and cell separation. Active on M.luteus cell walls and on E.hirae cell wall fractions, but not active on E.hirae intact cell walls. Can covalently bind penicillin.2 Publications

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

    GO - Molecular functioni

    1. amidase activity Source: InterPro
    2. lysozyme activity Source: UniProtKB-EC

    GO - Biological processi

    1. barrier septum assembly Source: UniProtKB-KW
    2. cell wall macromolecule metabolic process Source: InterPro
    3. cytolysis Source: UniProtKB-KW
    4. defense response to bacterium Source: UniProtKB-KW
    5. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell cycle, Cell division, Cell wall biogenesis/degradation, Septation

    Protein family/group databases

    CAZyiCBM50. Carbohydrate-Binding Module Family 50.
    GH73. Glycoside Hydrolase Family 73.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muramidase-2 (EC:3.2.1.17)
    Alternative name(s):
    1,4-beta-N-acetylmuramoylhydrolase
    Lysozyme
    Peptidoglycan hydrolase
    Pg-hydrolase 2
    Gene namesi
    Ordered Locus Names:EHR_05900
    OrganismiEnterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
    Taxonomic identifieri768486 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
    ProteomesiUP000002895: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 49491 PublicationAdd
    BLAST
    Chaini50 – 666617Muramidase-2PRO_0000012120Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP39046.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati257 – 29943LysM 1Add
    BLAST
    Repeati338 – 38043LysM 2Add
    BLAST
    Repeati414 – 45643LysM 3Add
    BLAST
    Repeati489 – 53143LysM 4Add
    BLAST
    Repeati565 – 60743LysM 5Add
    BLAST
    Repeati623 – 66543LysM 6Add
    BLAST

    Domaini

    LysM repeats are thought to be involved in peptidoglycan binding.

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 73 family.Curated
    Contains 6 LysM repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di3.10.350.10. 6 hits.
    InterProiIPR018392. LysM_dom.
    IPR013338. Lysozyme_subfam2_dom.
    IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
    [Graphical view]
    PfamiPF01832. Glucosaminidase. 1 hit.
    PF01476. LysM. 6 hits.
    [Graphical view]
    SMARTiSM00257. LysM. 6 hits.
    SM00047. LYZ2. 1 hit.
    [Graphical view]
    SUPFAMiSSF54106. SSF54106. 6 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39046-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENIARKERR RLNETKRFRK VKRSAALVGT AMVGCSVAAP LIQPVQVDAD    50
    QTPTQFGARI NTAAFIAEIA TYAQPIAQAN DLYASVMIAQ AVVESGWGSS 100
    ALSQAPYYNL FGIKGSYQGQ TVYMDTLEYL NNKWVSKKEP FRQYPSFAES 150
    FNDNAYVLRN TSFGNGYYYA GTWKSNTKSY TDATACLTGR YATDPGYAGK 200
    LNNIITTYGL TKYDTPASGN AGGGVTIGNG GNTGNTSNSG STSGNSGGSA 250
    TTTGTTYTVK SGDSVWGISH SFGITMAQLI EWNNIKNNFI YPGQKLTIKG 300
    GQSAGSSTTN TGNNASSGNT SGNTNTSGST GQATGAKYTV KSGDSVWKIA 350
    NDHGISMNQL IEWNNIKNNF VYPGQQLVVS KGSSSASGST SNTSTGNTSS 400
    NTANTGSTTS GSTYTVKAGE SVWSVSNKFG ISMNQLIQWN NIKNNFIYPG 450
    QKLIVKGGSS SSNASTSTAN NKNTASSNTS STATGQATYT VKAGESVWGV 500
    ANKNGISMNQ LIEWNNIKNN FIYPGQKLIV KGGSSKASAT ATIKPTASTP 550
    ASTTPTASST GDTKYTVKAG ESVWGVANKH HITMDQLIEW NNIKNNFIYP 600
    GQEVIVKKGT AQSTPAKSDE KTYTVKAGES VWGVADSHGI TMNQLIEWNN 650
    IKNNFIYPGQ QLIVKK 666
    Length:666
    Mass (Da):70,670
    Last modified:February 1, 1995 - v1
    Checksum:iFF0A7FAFCD810BA3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77639 Genomic DNA. Translation: AAA24776.1.
    CP003504 Genomic DNA. Translation: AFM70129.1.
    PIRiA42296.
    RefSeqiWP_010736969.1. NZ_KB946228.1.
    YP_006487020.1. NC_018081.1.

    Genome annotation databases

    EnsemblBacteriaiAFM70129; AFM70129; EHR_05900.
    GeneIDi13177133.
    KEGGiehr:EHR_05900.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77639 Genomic DNA. Translation: AAA24776.1 .
    CP003504 Genomic DNA. Translation: AFM70129.1 .
    PIRi A42296.
    RefSeqi WP_010736969.1. NZ_KB946228.1.
    YP_006487020.1. NC_018081.1.

    3D structure databases

    ProteinModelPortali P39046.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM50. Carbohydrate-Binding Module Family 50.
    GH73. Glycoside Hydrolase Family 73.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AFM70129 ; AFM70129 ; EHR_05900 .
    GeneIDi 13177133.
    KEGGi ehr:EHR_05900.

    Family and domain databases

    Gene3Di 3.10.350.10. 6 hits.
    InterProi IPR018392. LysM_dom.
    IPR013338. Lysozyme_subfam2_dom.
    IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
    [Graphical view ]
    Pfami PF01832. Glucosaminidase. 1 hit.
    PF01476. LysM. 6 hits.
    [Graphical view ]
    SMARTi SM00257. LysM. 6 hits.
    SM00047. LYZ2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54106. SSF54106. 6 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of the muramidase-2 gene from Enterococcus hirae."
      Chu C.-P., Kariyama R., Daneo-Moore L., Shockman G.D.
      J. Bacteriol. 174:1619-1625(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 50-73.
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
    2. "Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis."
      Gaechter T., Wunderlin C., Schmidheini T., Solioz M.
      J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
    3. "The second peptidoglycan hydrolase of Streptococcus faecium ATCC 9790 covalently binds penicillin."
      Dolinger D.L., Daneo-Moore L., Shockman G.D.
      J. Bacteriol. 171:4355-4361(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
    4. "Thermosensitive cell growth mutants of Enterococcus hirae that elongate at non-permissive temperature are stimulated to divide by parental autolytic enzymes."
      Del Mar Lleo M., Canepari P., Satta G.
      J. Gen. Microbiol. 139:3099-3117(1993)
      Cited for: FUNCTION.
      Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.

    Entry informationi

    Entry nameiMUR2_ENTHA
    AccessioniPrimary (citable) accession number: P39046
    Secondary accession number(s): I6TA70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3