SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P39046

- MUR2_ENTHA

UniProt

P39046 - MUR2_ENTHA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Muramidase-2

Gene
EHR_05900
Organism
Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

May work in concert with and potentiate the processive hydrolytic action of muramidase-1, which requires binding of the enzyme to non-reducing ends of glycan chains. Hydrolysis in the midst of glycan chains would increase the number of binding sites for muramidase-1. May function in facilitating septum formation and cell separation. Active on M.luteus cell walls and on E.hirae cell wall fractions, but not active on E.hirae intact cell walls. Can covalently bind penicillin.2 Publications

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

GO - Molecular functioni

  1. amidase activity Source: InterPro
  2. lysozyme activity Source: UniProtKB-EC

GO - Biological processi

  1. barrier septum assembly Source: UniProtKB-KW
  2. cell wall macromolecule metabolic process Source: InterPro
  3. cytolysis Source: UniProtKB-KW
  4. defense response to bacterium Source: UniProtKB-KW
  5. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Cell wall biogenesis/degradation, Septation

Protein family/group databases

CAZyiCBM50. Carbohydrate-Binding Module Family 50.
GH73. Glycoside Hydrolase Family 73.

Names & Taxonomyi

Protein namesi
Recommended name:
Muramidase-2 (EC:3.2.1.17)
Alternative name(s):
1,4-beta-N-acetylmuramoylhydrolase
Lysozyme
Peptidoglycan hydrolase
Pg-hydrolase 2
Gene namesi
Ordered Locus Names:EHR_05900
OrganismiEnterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
Taxonomic identifieri768486 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000002895: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 49491 PublicationAdd
BLAST
Chaini50 – 666617Muramidase-2PRO_0000012120Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP39046.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati257 – 29943LysM 1Add
BLAST
Repeati338 – 38043LysM 2Add
BLAST
Repeati414 – 45643LysM 3Add
BLAST
Repeati489 – 53143LysM 4Add
BLAST
Repeati565 – 60743LysM 5Add
BLAST
Repeati623 – 66543LysM 6Add
BLAST

Domaini

LysM repeats are thought to be involved in peptidoglycan binding.

Sequence similaritiesi

Contains 6 LysM repeats.

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di3.10.350.10. 6 hits.
InterProiIPR018392. LysM_dom.
IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
[Graphical view]
PfamiPF01832. Glucosaminidase. 1 hit.
PF01476. LysM. 6 hits.
[Graphical view]
SMARTiSM00257. LysM. 6 hits.
SM00047. LYZ2. 1 hit.
[Graphical view]
SUPFAMiSSF54106. SSF54106. 6 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39046-1 [UniParc]FASTAAdd to Basket

« Hide

MENIARKERR RLNETKRFRK VKRSAALVGT AMVGCSVAAP LIQPVQVDAD    50
QTPTQFGARI NTAAFIAEIA TYAQPIAQAN DLYASVMIAQ AVVESGWGSS 100
ALSQAPYYNL FGIKGSYQGQ TVYMDTLEYL NNKWVSKKEP FRQYPSFAES 150
FNDNAYVLRN TSFGNGYYYA GTWKSNTKSY TDATACLTGR YATDPGYAGK 200
LNNIITTYGL TKYDTPASGN AGGGVTIGNG GNTGNTSNSG STSGNSGGSA 250
TTTGTTYTVK SGDSVWGISH SFGITMAQLI EWNNIKNNFI YPGQKLTIKG 300
GQSAGSSTTN TGNNASSGNT SGNTNTSGST GQATGAKYTV KSGDSVWKIA 350
NDHGISMNQL IEWNNIKNNF VYPGQQLVVS KGSSSASGST SNTSTGNTSS 400
NTANTGSTTS GSTYTVKAGE SVWSVSNKFG ISMNQLIQWN NIKNNFIYPG 450
QKLIVKGGSS SSNASTSTAN NKNTASSNTS STATGQATYT VKAGESVWGV 500
ANKNGISMNQ LIEWNNIKNN FIYPGQKLIV KGGSSKASAT ATIKPTASTP 550
ASTTPTASST GDTKYTVKAG ESVWGVANKH HITMDQLIEW NNIKNNFIYP 600
GQEVIVKKGT AQSTPAKSDE KTYTVKAGES VWGVADSHGI TMNQLIEWNN 650
IKNNFIYPGQ QLIVKK 666
Length:666
Mass (Da):70,670
Last modified:February 1, 1995 - v1
Checksum:iFF0A7FAFCD810BA3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M77639 Genomic DNA. Translation: AAA24776.1.
CP003504 Genomic DNA. Translation: AFM70129.1.
PIRiA42296.
RefSeqiWP_010736969.1. NZ_KB946228.1.
YP_006487020.1. NC_018081.1.

Genome annotation databases

EnsemblBacteriaiAFM70129; AFM70129; EHR_05900.
GeneIDi13177133.
KEGGiehr:EHR_05900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M77639 Genomic DNA. Translation: AAA24776.1 .
CP003504 Genomic DNA. Translation: AFM70129.1 .
PIRi A42296.
RefSeqi WP_010736969.1. NZ_KB946228.1.
YP_006487020.1. NC_018081.1.

3D structure databases

ProteinModelPortali P39046.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM50. Carbohydrate-Binding Module Family 50.
GH73. Glycoside Hydrolase Family 73.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFM70129 ; AFM70129 ; EHR_05900 .
GeneIDi 13177133.
KEGGi ehr:EHR_05900.

Family and domain databases

Gene3Di 3.10.350.10. 6 hits.
InterProi IPR018392. LysM_dom.
IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
[Graphical view ]
Pfami PF01832. Glucosaminidase. 1 hit.
PF01476. LysM. 6 hits.
[Graphical view ]
SMARTi SM00257. LysM. 6 hits.
SM00047. LYZ2. 1 hit.
[Graphical view ]
SUPFAMi SSF54106. SSF54106. 6 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of the muramidase-2 gene from Enterococcus hirae."
    Chu C.-P., Kariyama R., Daneo-Moore L., Shockman G.D.
    J. Bacteriol. 174:1619-1625(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 50-73.
    Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
  2. "Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis."
    Gaechter T., Wunderlin C., Schmidheini T., Solioz M.
    J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
  3. "The second peptidoglycan hydrolase of Streptococcus faecium ATCC 9790 covalently binds penicillin."
    Dolinger D.L., Daneo-Moore L., Shockman G.D.
    J. Bacteriol. 171:4355-4361(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
  4. "Thermosensitive cell growth mutants of Enterococcus hirae that elongate at non-permissive temperature are stimulated to divide by parental autolytic enzymes."
    Del Mar Lleo M., Canepari P., Satta G.
    J. Gen. Microbiol. 139:3099-3117(1993)
    Cited for: FUNCTION.
    Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.

Entry informationi

Entry nameiMUR2_ENTHA
AccessioniPrimary (citable) accession number: P39046
Secondary accession number(s): I6TA70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi