P39016 (MPT5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Suppressor protein MPT5

Alternative name(s):
Protein HTR1
Pumilio homology domain family member 5

Gene names

Name:	MPT5
Synonyms:	HTR1, PUF5
Ordered Locus Names:	YGL178W
ORF Names:	BIC834

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-binding protein involved in post-transcriptional regulation. Negatively regulates expression of HO by binding to the 3'-UTR of HO mRNA. Predominantly binds to mRNAs encoding chromatin modifiers and spindle pole body components. Recognizes and binds to 5'-TGTAA[CT]A[AT]TA-3' in the 3'-UTR of target mRNAs. Multicopy suppressor of POP2 mutation. Required for high temperature growth. Ref.7
Subcellular location	Cytoplasm Ref.9.
Miscellaneous	Present with 1240 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Contains 1 PUM-HD domain. Contains 8 pumilio repeats.
Sequence caution	The sequence BAA05024.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAA05172.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA58660.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA58663.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fungal-type cell wall organization Inferred from mutant phenotype. Source: SGD negative regulation of chromatin silencing involved in replicative cell aging Inferred from genetic interaction. Source: SGD negative regulation of translation Inferred from direct assay. Source: SGD nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Inferred from genetic interaction. Source: SGD protein localization Inferred from mutant phenotype. Source: SGD re-entry into mitotic cell cycle after pheromone arrest Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	mRNA binding Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 859	859	Suppressor protein MPT5		PRO_0000075923
Regions
	Domain	188 – 596	409	PUM-HD
	Repeat	209 – 247	39	Pumilio 1
	Repeat	248 – 283	36	Pumilio 2
	Repeat	284 – 320	37	Pumilio 3
	Repeat	325 – 362	38	Pumilio 4
	Repeat	363 – 400	38	Pumilio 5
	Repeat	401 – 438	38	Pumilio 6
	Repeat	439 – 474	36	Pumilio 7
	Repeat	503 – 539	37	Pumilio 8
	Compositional bias	647 – 653	7	Poly-Asn
Amino acid modifications
	Modified residue	138	1	Phosphoserine Ref.12
	Modified residue	139	1	Phosphoserine Ref.12
	Modified residue	176	1	Phosphoserine Ref.12
	Modified residue	662	1	Phosphoserine Ref.10 Ref.12
	Modified residue	675	1	Phosphoserine Ref.12
	Modified residue	749	1	Phosphoserine Ref.12
	Modified residue	753	1	Phosphoserine Ref.12
	Modified residue	756	1	Phosphothreonine Ref.12
	Modified residue	834	1	Phosphoserine Ref.11
	Modified residue	838	1	Phosphoserine Ref.12

Sequences

	Sequence		Length	Mass (Da)
	P39016 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 6B289BAF3CEA2200	FASTA	859	95,422
	10 20 30 40 50 60 MINNEPFPSA DSASILTTST SNNSLMSYNH QPQLSINSVQ SLLEPVTPPP LGQMNNKRNH 70 80 90 100 110 120 QKAHSLDLSG FNQFISSTQS PLALMNNTST SNSANSFSPN PNAASNSTGL SASMANPPAI 130 140 150 160 170 180 LPLINEFDLE MDGPRRKSSH DFTVVAPSNS GVNTSSLIME TPSSSVTPAA SLRNFSNSNN 190 200 210 220 230 240 AASKCGVDNS SFGLSSSTSS SMVEISALPL RDLDYIKLAT DQFGCRFLQK KLETPSESNM 250 260 270 280 290 300 VRDLMYEQIK PFFLDLILDP FGNYLVQKLC DYLTAEQKTL LIQTIYPNVF QISINQYGTR 310 320 330 340 350 360 SLQKIIDTVD NEVQIDLIIK GFSQEFTSIE QVVTLINDLN GNHVIQKCIF KFSPSKFGFI 370 380 390 400 410 420 IDAIVEQNNI ITISTHKHGC CVLQKLLSVC TLQQIFKISV KIVQFLPGLI NDQFGNYIIQ 430 440 450 460 470 480 FLLDIKELDF YLLAELFNRL SNELCQLSCL KFSSNVVEKF IKKLFRIITG FIVNNNGGAS 490 500 510 520 530 540 QRTAVASDDV INASMNILLT TIDIFTVNLN VLIRDNFGNY ALQTLLDVKN YSPLLAYNKN 550 560 570 580 590 600 SNAIGQNSSS TLNYGNFCND FSLKIGNLIV LTKELLPSIK TTSYAKKIKL KVKAYAEATG 610 620 630 640 650 660 IPFTDISPQV TAMSHNNLQT INNENKNPHN KNSHNHNHNH NHNHAHNNNN NNNQKSHTRH 670 680 690 700 710 720 FSLPANAYHR RSNSSVTNNF SNQYAQDQKI HSPQQIMNFN QNAYPSMGAP SFNSQTNPPL 730 740 750 760 770 780 VSHNSLQNFD NRQFANLMAH PNSAAPIHSF SSSNITNVNP NVSRGFKQPG FMMNETDKIN 790 800 810 820 830 840 ANHFSPYSNA NSQNFNESFV PRMQYQTEGA NWDSSLSMKS QHIGQGPYNQ VNMSRNASIS 850 NMPAMNTART SDELQFTLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence of a 7941 bp fragment of the left arm of chromosome VII of Saccharomyces cerevisiae contains four open reading frames including the multicopy suppressor gene of the pop2 mutation and a putative serine/threonine protein kinase gene." Coglievina M., Bertani I., Klima R., Zaccaria P., Bruschi C.V. Yeast 11:767-774(1995) [PubMed: 7668046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A new yeast gene, HTR1, required for growth at high temperature, is needed for recovery from mating pheromone-induced G1 arrest." Kikuchi Y., Oka Y., Kobayashi M., Uesono Y., Toh-e A., Kikuchi A. Mol. Gen. Genet. 245:107-116(1994) [PubMed: 7845352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-859. Strain: ATCC 70391.
[5]	"Multicopy suppressors of the yeast pop2 mutation." Sakai A. Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-859. Strain: ATCC 204508 / S288c.
[6]	"Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast." Davis C.A., Grate L., Spingola M., Ares M. Jr. Nucleic Acids Res. 28:1700-1706(2000) [PubMed: 10734188] [Abstract] Cited for: IDENTIFICATION OF INTRON.
[7]	"Post-transcriptional regulation through the HO 3'-UTR by Mpt5, a yeast homolog of Pumilio and FBF." Tadauchi T., Matsumoto K., Herskowitz I., Irie K. EMBO J. 20:552-561(2001) [PubMed: 11157761] [Abstract] Cited for: FUNCTION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast." Gerber A.P., Herschlag D., Brown P.O. PLoS Biol. 2:342-354(2004) [PubMed: 15024427] [Abstract] Cited for: RNA-BINDING, SUBCELLULAR LOCATION.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-662, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-834, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138; SER-139; SER-176; SER-662; SER-675; SER-749; SER-753; THR-756 AND SER-838, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83690 Genomic DNA. Translation: CAA58660.1. Sequence problems. X83690 Genomic DNA. Translation: CAA58663.1. Sequence problems. Z72700 Genomic DNA. Translation: CAA96889.1. D25541 Genomic DNA. Translation: BAA05024.1. Sequence problems. D26184 Genomic DNA. Translation: BAA05172.1. Sequence problems. BK006941 Genomic DNA. Translation: DAA07936.1.
PIR	S64195.
RefSeq	NP_011337.1. NM_001181043.1.
3D structure databases
ProteinModelPortal	P39016.
SMR	P39016. Positions 202-593.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2271N.
IntAct	P39016. 9 interactions.
MINT	MINT-2779525.
STRING	P39016.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL178W; YGL178W; YGL178W.
GeneID	852697.
KEGG	sce:YGL178W.
NMPDR	fig|4932.3.peg.2441.
Organism-specific databases
CYGD	YGL178w.
SGD	S000003146. MPT5.
Phylogenomic databases
eggNOG	fuNOG05346.
GeneTree	EFGT00050000001630.
HOGENOM	HBG204462.
OMA	ISINQYG.
OrthoDB	EOG42NN7Z.
Gene expression databases
ArrayExpress	P39016.
Genevestigator	P39016.
GermOnline	YGL178W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR001313. Pumilio_RNA-bd_rpt. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 2 hits.
Pfam	PF00806. PUF. 7 hits. [Graphical view]
SMART	SM00025. Pumilio. 8 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50302. PUM. 8 hits. PS50303. PUM_HD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972038.

Entry information

Entry name

MPT5_YEAST

Accession

Primary (citable) accession number: P39016
Secondary accession number(s): D6VTX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 17, 2006
Last modified:	December 14, 2011
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program