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P39014

- MET30_YEAST

UniProt

P39014 - MET30_YEAST

Protein

F-box protein MET30

Gene

MET30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Negatively regulates sulfur amino acids biosynthesis genes expression. Controls cell cycle function (being required for the G1/S transition and M-phase but not the S-phase), sulfur metabolism, and methionine biosynthesis as part of the E3 ubiquitin ligase complex SCF(Met30). Required for the efficient binding of CDC45 and MCM proteins to origins of replication. Involved in the S-adenosylmethionine (AdoMet)-mediated inhibition of the transcription function of MET4. In the context of the E3 ubiquitin ligase complex SCF(Met30), involved in the degradation of MET4 and the cellular response to cadmium. Required for efficient expression of G1 cyclins.5 Publications

    Pathwayi

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct
    3. ubiquitin binding Source: SGD

    GO - Biological processi

    1. cysteine biosynthetic process Source: UniProtKB-KW
    2. DNA replication initiation Source: SGD
    3. G1/S transition of mitotic cell cycle Source: SGD
    4. methionine biosynthetic process Source: UniProtKB-KW
    5. protein polyubiquitination Source: SGD
    6. protein ubiquitination Source: SGD
    7. regulation of DNA-dependent DNA replication initiation Source: SGD
    8. regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
    9. response to arsenic-containing substance Source: SGD
    10. response to cadmium ion Source: SGD
    11. SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
    12. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Amino-acid biosynthesis, Cell cycle, Cysteine biosynthesis, Methionine biosynthesis, Transcription, Transcription regulation, Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31317-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    F-box protein MET30
    Alternative name(s):
    E3 ubiquitin ligase complex SCF(Met30) subunit MET30
    Methionine-requiring protein 30
    Gene namesi
    Name:MET30
    Ordered Locus Names:YIL046W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL046w.
    SGDiS000001308. MET30.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nuclear SCF ubiquitin ligase complex Source: SGD
    3. nucleus Source: SGD
    4. SCF ubiquitin ligase complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi187 – 1871L → D: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. 1 Publication
    Mutagenesisi190 – 1901E → A: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. 1 Publication
    Mutagenesisi386 – 3861L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425 and A-467. 1 Publication
    Mutagenesisi425 – 4251N → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-530 or D-386 and A-467. 1 Publication
    Mutagenesisi467 – 4671Q → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-386 and A-425. 1 Publication
    Mutagenesisi530 – 5301L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 640640F-box protein MET30PRO_0000051087Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei67 – 671Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39014.
    PaxDbiP39014.
    PeptideAtlasiP39014.

    Expressioni

    Inductioni

    Transcriptional activation requires MET4 as well as MET31 and MET32. Regulated by intracellular AdoMet levels. L-methionine regulates the abundance of MET30. The amount of MET30 regulates the activity of the E3 ubiquitin ligase complex SCF(Met30).2 Publications

    Gene expression databases

    GenevestigatoriP39014.

    Interactioni

    Subunit structurei

    Homomultimer. Interacts with MET4. Interacts with CDC53 and SKP1/CBF3D to form the E3 ubiquitin ligase complex SCF(Met30).5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-11507,EBI-11507
    CDC53Q120189EBI-11507,EBI-4321
    MET4P323896EBI-11507,EBI-10757
    SKP1P5228613EBI-11507,EBI-4090
    SRL2Q120203EBI-11507,EBI-38714

    Protein-protein interaction databases

    BioGridi34944. 107 interactions.
    DIPiDIP-1439N.
    IntActiP39014. 74 interactions.
    MINTiMINT-385167.
    STRINGi4932.YIL046W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39014.
    SMRiP39014. Positions 167-590.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini181 – 22747F-boxPROSITE-ProRule annotationAdd
    BLAST
    Repeati300 – 32829WD 1Add
    BLAST
    Repeati340 – 36829WD 2Add
    BLAST
    Repeati380 – 40829WD 3Add
    BLAST
    Repeati419 – 44931WD 4Add
    BLAST
    Repeati461 – 49939WD 5Add
    BLAST
    Repeati509 – 53830WD 6Add
    BLAST
    Repeati550 – 57829WD 7Add
    BLAST
    Repeati607 – 63529WD 8Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 299299Necessary to mediate nuclear localizationAdd
    BLAST
    Regioni180 – 27798Important for mediating homomultimerizationAdd
    BLAST
    Regioni180 – 22546Interaction with SKP1/CBF3DAdd
    BLAST
    Regioni277 – 640364Interaction with MET4Add
    BLAST

    Sequence similaritiesi

    Contains 1 F-box domain.PROSITE-ProRule annotation
    Contains 8 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00710000106459.
    HOGENOMiHOG000166480.
    KOiK10259.
    OMAiYHTGECI.
    OrthoDBiEOG7C5MHX.

    Family and domain databases

    Gene3Di2.130.10.10. 3 hits.
    InterProiIPR001810. F-box_dom.
    IPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00646. F-box. 1 hit.
    PF12937. F-box-like. 1 hit.
    PF00400. WD40. 6 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00256. FBOX. 1 hit.
    SM00320. WD40. 6 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.
    SSF81383. SSF81383. 1 hit.
    PROSITEiPS50181. FBOX. 1 hit.
    PS00678. WD_REPEATS_1. 4 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39014-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRERQRMMS FEDKDKDDLD NSNSNNSSEM TDTAMMPPLK RLLITGSSDD    50
    LAQGSSGKKK MTMATRSPSS SPDLATNDSG TRVQPLPEYN FTKFCYRHNP 100
    DIQFSPTHTA CYKQDLKRTQ EINANIAKLP LQEQSDIHHI ISKYSNSNDK 150
    IRKLILDGIL STSCFPQLSY ISSLVTHMIK IDFISILPQE LSLKILSYLD 200
    CQSLCNATRV CRKWQKLADD DRVWYHMCEQ HIDRKCPNCG WGLPLLHMKR 250
    ARIQQNSTGS SSNADIQTQT TRPWKVIYRE RFKVESNWRK GHCRIQEFKG 300
    HMDGVLTLQF NYRLLFTGSY DSTIGIWDLF TGKLIRRLSG HSDGVKTLYF 350
    DDRKLITGSL DKTIRVWNYI TGECISTYRG HSDSVLSVDS YQKVIVSGSA 400
    DKTVKVWHVE SRTCYTLRGH TEWVNCVKLH PKSFSCFSCS DDTTIRMWDI 450
    RTNSCLKVFR GHVGQVQKII PLTIKDVENL ATDNTSDGSS PQDDPTMTDG 500
    ADESDTPSNE QETVLDENIP YPTHLLSCGL DNTIKLWDVK TGKCIRTQFG 550
    HVEGVWDIAA DNFRIISGSH DGSIKVWDLQ SGKCMHTFNG RRLQRETQHT 600
    QTQSLGDKVA PIACVCIGDS ECFSGDEFGC VKMYKFDLND 640
    Length:640
    Mass (Da):72,835
    Last modified:February 1, 1995 - v1
    Checksum:i5135D4BCA2E1EB97
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti61 – 611M → I in AAA96717. (PubMed:8524217)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46861 Genomic DNA. Translation: CAA86905.1.
    L26505 Genomic DNA. Translation: AAA96717.1.
    BK006942 Genomic DNA. Translation: DAA08502.1.
    PIRiS49932.
    RefSeqiNP_012218.1. NM_001179396.1.

    Genome annotation databases

    EnsemblFungiiYIL046W; YIL046W; YIL046W.
    GeneIDi854765.
    KEGGisce:YIL046W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46861 Genomic DNA. Translation: CAA86905.1 .
    L26505 Genomic DNA. Translation: AAA96717.1 .
    BK006942 Genomic DNA. Translation: DAA08502.1 .
    PIRi S49932.
    RefSeqi NP_012218.1. NM_001179396.1.

    3D structure databases

    ProteinModelPortali P39014.
    SMRi P39014. Positions 167-590.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34944. 107 interactions.
    DIPi DIP-1439N.
    IntActi P39014. 74 interactions.
    MINTi MINT-385167.
    STRINGi 4932.YIL046W.

    Proteomic databases

    MaxQBi P39014.
    PaxDbi P39014.
    PeptideAtlasi P39014.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL046W ; YIL046W ; YIL046W .
    GeneIDi 854765.
    KEGGi sce:YIL046W.

    Organism-specific databases

    CYGDi YIL046w.
    SGDi S000001308. MET30.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00710000106459.
    HOGENOMi HOG000166480.
    KOi K10259.
    OMAi YHTGECI.
    OrthoDBi EOG7C5MHX.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-31317-MONOMER.

    Miscellaneous databases

    NextBioi 977517.

    Gene expression databases

    Genevestigatori P39014.

    Family and domain databases

    Gene3Di 2.130.10.10. 3 hits.
    InterProi IPR001810. F-box_dom.
    IPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00646. F-box. 1 hit.
    PF12937. F-box-like. 1 hit.
    PF00400. WD40. 6 hits.
    [Graphical view ]
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00256. FBOX. 1 hit.
    SM00320. WD40. 6 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    SSF81383. SSF81383. 1 hit.
    PROSITEi PS50181. FBOX. 1 hit.
    PS00678. WD_REPEATS_1. 4 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats."
      Thomas D., Kuras L., Barbey R., Cherest H., Blaiseau P.L., Surdin-Kerjan Y.
      Mol. Cell. Biol. 15:6526-6534(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH MET4.
      Strain: ATCC 26786 / X2180-1A.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast."
      Patton E.E., Willems A.R., Sa D., Kuras L., Thomas D., Craig K.L., Tyers M.
      Genes Dev. 12:692-705(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKP1/CBF3D AND CDC53.
    5. "Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30) complex."
      Rouillon A., Barbey R., Patton E.E., Tyers M., Thomas D.
      EMBO J. 19:282-294(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH MET4 AND SKP1/CBF3D.
    6. "The abundance of Met30p limits SCF(Met30p) complex activity and is regulated by methionine availability."
      Smothers D.B., Kozubowski L., Dixon C., Goebl M.G., Mathias N.
      Mol. Cell. Biol. 20:7845-7852(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "The amino-terminal portion of the F-box protein Met30p mediates its nuclear import and assimilation into an SCF complex."
      Brunson L.E., Dixon C., Kozubowski L., Mathias N.
      J. Biol. Chem. 279:6674-6682(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SKP1/CBF3D, HOMOMULTIMERIZATION, MUTAGENESIS OF LEU-187 AND GLU-190.
    10. "The yeast ubiquitin ligase SCFMet30 regulates heavy metal response."
      Yen J.L., Su N.Y., Kaiser P.
      Mol. Biol. Cell 16:1872-1882(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "The F-box protein Met30 is required for multiple steps in the budding yeast cell cycle."
      Su N.Y., Flick K., Kaiser P.
      Mol. Cell. Biol. 25:3875-3885(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p."
      Brunson L.E., Dixon C., LeFebvre A., Sun L., Mathias N.
      Mol. Genet. Genomics 273:361-370(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MET4, MUTAGENESIS OF LEU-386; ASN-425; GLN-467 AND LEU-530.
    13. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMET30_YEAST
    AccessioniPrimary (citable) accession number: P39014
    Secondary accession number(s): D6VVN6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 146 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 217 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3