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Reviewed, UniProtKB/Swiss-Prot P39014 (MET30_YEAST)

Last modified June 16, 2009. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    F-box protein MET30
Alternative name(s):
    Methionine-requiring protein 30
    E3 ubiquitin ligase complex SCF(Met30) subunit MET30
Gene names
Name: MET30
Ordered Locus Names: YIL046W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Negatively regulates sulfur amino acids biosynthesis genes expression. Controls cell cycle function (being required for the G1/S transition and M-phase but not the S-phase), sulfur metabolism, and methionine biosynthesis as part of the E3 ubiquitin ligase complex SCF(Met30). Required for the efficient binding of CDC45 and MCM proteins to origins of replication. Involved in the S-adenosylmethionine (AdoMet)-mediated inhibition of the transcription function of MET4. In the context of the E3 ubiquitin ligase complex SCF(Met30), involved in the degradation of MET4 and the cellular response to cadmium. Required for efficient expression of G1 cyclins. Ref.1 Ref.4 Ref.8 Ref.9 Ref.10

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Homomultimer. Interacts with MET4. Interacts with CDC53 and SKP1/CBF3D to form the E3 ubiquitin ligase complex SCF(Met30). Ref.1 Ref.4 Ref.8 Ref.3 Ref.11

Subcellular location

Cytoplasm. Nucleus. Ref.4 Ref.8 Ref.6

Induction

Transcriptional activation requires MET4 as well as MET31 and MET32. Regulated by intracellular AdoMet levels. L-methionine regulates the abundance of MET30. The amount of MET30 regulates the activity of the E3 ubiquitin ligase complex SCF(Met30). Ref.4 Ref.5

Miscellaneous

Present with 217 molecules/cell in log phase SD medium. Ref.7

Sequence similarities

Belongs to the WD repeat MET30/SCONB/SCON-2 family.

Contains 1 F-box domain.

Contains 8 WD repeats.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Cell cycle
Cysteine biosynthesis
Methionine biosynthesis
Transcription
Transcription regulation
Ubl conjugation pathway
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
WD repeat
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processG1/S transition of mitotic cell cycle Ref.10

Inferred from mutant phenotype. Source: SGD

M phase of mitotic cell cycle Ref.10

Inferred from mutant phenotype. Source: SGD

SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Inferred from direct assay. Source: SGD

cysteine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

methionine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

protein ubiquitination Ref.3

Inferred from genetic interaction. Source: SGD

regulation of DNA replication initiation Ref.10

Inferred from mutant phenotype. Source: SGD

regulation of transcription during G1 phase of mitotic cell cycle Ref.10

Inferred from mutant phenotype. Source: SGD

response to arsenic Ref.9

Inferred from direct assay. Source: SGD

response to cadmium ion Ref.9

Inferred from direct assay. Source: SGD

transcription

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nuclear SCF ubiquitin ligase complex Ref.4

Inferred from direct assay. Source: SGD

   Molecular functionidentical protein binding Ref.8

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 640640F-box protein MET30
PRO_0000051087

Regions

Domain181 – 22747F-box
Repeat300 – 32829WD 1
Repeat340 – 36829WD 2
Repeat380 – 40829WD 3
Repeat419 – 44931WD 4
Repeat461 – 49939WD 5
Repeat509 – 53830WD 6
Repeat550 – 57829WD 7
Repeat607 – 63529WD 8
Region1 – 299299Necessary to mediate nuclear localization
Region180 – 27798Important for mediating homomultimerization
Region180 – 22546Interaction with SKP1/CBF3D
Region277 – 640364Interaction with MET4

Amino acid modifications

Modified residue471Phosphoserine Ref.12
Modified residue481Phosphoserine Ref.12
Modified residue691Phosphoserine Ref.12
Modified residue1051Phosphoserine Ref.12

Experimental info

Mutagenesis1871L → D: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. Ref.8
Mutagenesis1901E → A: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. Ref.8
Mutagenesis3861L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425 and A-467. Ref.11
Mutagenesis4251N → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-530 or D-386 and A-467. Ref.11
Mutagenesis4671Q → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-386 and A-425. Ref.11
Mutagenesis5301L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425. Ref.11
Sequence conflict611M → I in AAA96717. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39014-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 5135D4BCA2E1EB97

FASTA64072,835
        10         20         30         40         50         60 
MRRERQRMMS FEDKDKDDLD NSNSNNSSEM TDTAMMPPLK RLLITGSSDD LAQGSSGKKK 

        70         80         90        100        110        120 
MTMATRSPSS SPDLATNDSG TRVQPLPEYN FTKFCYRHNP DIQFSPTHTA CYKQDLKRTQ 

       130        140        150        160        170        180 
EINANIAKLP LQEQSDIHHI ISKYSNSNDK IRKLILDGIL STSCFPQLSY ISSLVTHMIK 

       190        200        210        220        230        240 
IDFISILPQE LSLKILSYLD CQSLCNATRV CRKWQKLADD DRVWYHMCEQ HIDRKCPNCG 

       250        260        270        280        290        300 
WGLPLLHMKR ARIQQNSTGS SSNADIQTQT TRPWKVIYRE RFKVESNWRK GHCRIQEFKG 

       310        320        330        340        350        360 
HMDGVLTLQF NYRLLFTGSY DSTIGIWDLF TGKLIRRLSG HSDGVKTLYF DDRKLITGSL 

       370        380        390        400        410        420 
DKTIRVWNYI TGECISTYRG HSDSVLSVDS YQKVIVSGSA DKTVKVWHVE SRTCYTLRGH 

       430        440        450        460        470        480 
TEWVNCVKLH PKSFSCFSCS DDTTIRMWDI RTNSCLKVFR GHVGQVQKII PLTIKDVENL 

       490        500        510        520        530        540 
ATDNTSDGSS PQDDPTMTDG ADESDTPSNE QETVLDENIP YPTHLLSCGL DNTIKLWDVK 

       550        560        570        580        590        600 
TGKCIRTQFG HVEGVWDIAA DNFRIISGSH DGSIKVWDLQ SGKCMHTFNG RRLQRETQHT 

       610        620        630        640 
QTQSLGDKVA PIACVCIGDS ECFSGDEFGC VKMYKFDLND 

« Hide

References

« Hide 'large scale' references
[1]"Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats."
Thomas D., Kuras L., Barbey R., Cherest H., Blaiseau P.L., Surdin-Kerjan Y.
Mol. Cell. Biol. 15:6526-6534(1995) [PubMed: 8524217] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH MET4.
Strain: ATCC 26786 / X2180-1A.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed: 9169870] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast."
Patton E.E., Willems A.R., Sa D., Kuras L., Thomas D., Craig K.L., Tyers M.
Genes Dev. 12:692-705(1998) [PubMed: 9499404] [Abstract]
Cited for: INTERACTION WITH SKP1/CBF3D AND CDC53.
[4]"Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30) complex."
Rouillon A., Barbey R., Patton E.E., Tyers M., Thomas D.
EMBO J. 19:282-294(2000) [PubMed: 10637232] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH MET4 AND SKP1/CBF3D.
[5]"The abundance of Met30p limits SCF(Met30p) complex activity and is regulated by methionine availability."
Smothers D.B., Kozubowski L., Dixon C., Goebl M.G., Mathias N.
Mol. Cell. Biol. 20:7845-7852(2000) [PubMed: 11027256] [Abstract]
Cited for: INDUCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The amino-terminal portion of the F-box protein Met30p mediates its nuclear import and assimilation into an SCF complex."
Brunson L.E., Dixon C., Kozubowski L., Mathias N.
J. Biol. Chem. 279:6674-6682(2004) [PubMed: 14660673] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SKP1/CBF3D, HOMOMULTIMERIZATION, MUTAGENESIS OF LEU-187 AND GLU-190.
[9]"The yeast ubiquitin ligase SCFMet30 regulates heavy metal response."
Yen J.L., Su N.Y., Kaiser P.
Mol. Biol. Cell 16:1872-1882(2005) [PubMed: 15689486] [Abstract]
Cited for: FUNCTION.
[10]"The F-box protein Met30 is required for multiple steps in the budding yeast cell cycle."
Su N.Y., Flick K., Kaiser P.
Mol. Cell. Biol. 25:3875-3885(2005) [PubMed: 15870262] [Abstract]
Cited for: FUNCTION.
[11]"Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p."
Brunson L.E., Dixon C., LeFebvre A., Sun L., Mathias N.
Mol. Genet. Genomics 273:361-370(2005) [PubMed: 15883825] [Abstract]
Cited for: INTERACTION WITH MET4, MUTAGENESIS OF LEU-386; ASN-425; GLN-467 AND LEU-530.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; SER-48; SER-69 AND SER-105, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

Z46861 Genomic DNA. Translation: CAA86905.1.
L26505 Genomic DNA. Translation: AAA96717.1.
PIRS49932.
RefSeqNP_012218.1.

3D structure databases

HSSPHSSP built from PDB template 1ERJ based on UniProtKB P16649.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1439N.
IntActP39014. 78 interactions.

Proteomic databases

PeptideAtlasP39014.
PRIDEP39014.

Genome annotation databases

EnsemblYIL046W. Saccharomyces cerevisiae. [Contig view]
GeneID854765.
GenomeReviewsGene locus YIL046W in contig Z47047_GR.
KEGGsce:YIL046W.
NMPDRfig|4932.3.peg.1751.

Organism-specific databases

CYGDYIL046w.
SGDS000001308. MET30.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP39014.
OMAP39014. DDTTIRM.

Gene expression databases

GermOnlineYIL046W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001810. F-box.
IPR015943. WD40/YVTN_repeat-like.
IPR001680. WD40_repeat.
IPR019782. WD40_repeat_2.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_region.
IPR019781. WD40_repeat_sg.
[Graphical view]
Gene3DG3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PfamPF00646. F-box. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
ProDomPD000018. WD40. 4 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00256. FBOX. 1 hit.
SM00320. WD40. 6 hits.
[Graphical view]
PROSITEPS50181. FBOX. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio977517.

Entry information

Entry nameMET30_YEAST
AccessionPrimary (citable) accession number: P39014
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 16, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents