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Protein

F-box protein MET30

Gene

MET30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates sulfur amino acids biosynthesis genes expression. Controls cell cycle function (being required for the G1/S transition and M-phase but not the S-phase), sulfur metabolism, and methionine biosynthesis as part of the E3 ubiquitin ligase complex SCF(Met30). Required for the efficient binding of CDC45 and MCM proteins to origins of replication. Involved in the S-adenosylmethionine (AdoMet)-mediated inhibition of the transcription function of MET4. In the context of the E3 ubiquitin ligase complex SCF(Met30), involved in the degradation of MET4 and the cellular response to cadmium. Required for efficient expression of G1 cyclins.5 Publications

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • ubiquitin binding Source: SGD

GO - Biological processi

  • cysteine biosynthetic process Source: UniProtKB-KW
  • DNA replication initiation Source: SGD
  • G1/S transition of mitotic cell cycle Source: SGD
  • methionine biosynthetic process Source: UniProtKB-KW
  • protein polyubiquitination Source: SGD
  • protein ubiquitination Source: SGD
  • regulation of DNA-dependent DNA replication initiation Source: SGD
  • regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
  • response to arsenic-containing substance Source: SGD
  • response to cadmium ion Source: SGD
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processAmino-acid biosynthesis, Cell cycle, Cysteine biosynthesis, Methionine biosynthesis, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31317-MONOMER.
ReactomeiR-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-8951664. Neddylation.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box protein MET30
Alternative name(s):
E3 ubiquitin ligase complex SCF(Met30) subunit MET30
Methionine-requiring protein 30
Gene namesi
Name:MET30
Ordered Locus Names:YIL046W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL046W.
SGDiS000001308. MET30.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi187L → D: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. 1 Publication1
Mutagenesisi190E → A: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. 1 Publication1
Mutagenesisi386L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425 and A-467. 1 Publication1
Mutagenesisi425N → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-530 or D-386 and A-467. 1 Publication1
Mutagenesisi467Q → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-386 and A-425. 1 Publication1
Mutagenesisi530L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510871 – 640F-box protein MET30Add BLAST640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39014.
PRIDEiP39014.

PTM databases

iPTMnetiP39014.

Expressioni

Inductioni

Transcriptional activation requires MET4 as well as MET31 and MET32. Regulated by intracellular AdoMet levels. L-methionine regulates the abundance of MET30. The amount of MET30 regulates the activity of the E3 ubiquitin ligase complex SCF(Met30).2 Publications

Interactioni

Subunit structurei

Homomultimer. Interacts with MET4. Interacts with CDC53 and SKP1/CBF3D to form the E3 ubiquitin ligase complex SCF(Met30).5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi34944. 477 interactors.
DIPiDIP-1439N.
IntActiP39014. 92 interactors.
MINTiMINT-385167.
STRINGi4932.YIL046W.

Structurei

3D structure databases

ProteinModelPortaliP39014.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini181 – 227F-boxPROSITE-ProRule annotationAdd BLAST47
Repeati300 – 328WD 1Add BLAST29
Repeati340 – 368WD 2Add BLAST29
Repeati380 – 408WD 3Add BLAST29
Repeati419 – 449WD 4Add BLAST31
Repeati461 – 499WD 5Add BLAST39
Repeati509 – 538WD 6Add BLAST30
Repeati550 – 578WD 7Add BLAST29
Repeati607 – 635WD 8Add BLAST29

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 299Necessary to mediate nuclear localizationAdd BLAST299
Regioni180 – 277Important for mediating homomultimerizationAdd BLAST98
Regioni180 – 225Interaction with SKP1/CBF3DAdd BLAST46
Regioni277 – 640Interaction with MET4Add BLAST364

Sequence similaritiesi

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00760000119106.
HOGENOMiHOG000166480.
InParanoidiP39014.
KOiK10259.
OMAiTQCCFPQ.
OrthoDBiEOG092C10HM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR036047. F-box-like_dom_sf.
IPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR036322. WD40_repeat_dom_sf.
PfamiView protein in Pfam
PF12937. F-box-like. 1 hit.
PF00400. WD40. 5 hits.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiView protein in SMART
SM00256. FBOX. 1 hit.
SM00320. WD40. 6 hits.
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF81383. SSF81383. 1 hit.
PROSITEiView protein in PROSITE
PS50181. FBOX. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

P39014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRERQRMMS FEDKDKDDLD NSNSNNSSEM TDTAMMPPLK RLLITGSSDD
60 70 80 90 100
LAQGSSGKKK MTMATRSPSS SPDLATNDSG TRVQPLPEYN FTKFCYRHNP
110 120 130 140 150
DIQFSPTHTA CYKQDLKRTQ EINANIAKLP LQEQSDIHHI ISKYSNSNDK
160 170 180 190 200
IRKLILDGIL STSCFPQLSY ISSLVTHMIK IDFISILPQE LSLKILSYLD
210 220 230 240 250
CQSLCNATRV CRKWQKLADD DRVWYHMCEQ HIDRKCPNCG WGLPLLHMKR
260 270 280 290 300
ARIQQNSTGS SSNADIQTQT TRPWKVIYRE RFKVESNWRK GHCRIQEFKG
310 320 330 340 350
HMDGVLTLQF NYRLLFTGSY DSTIGIWDLF TGKLIRRLSG HSDGVKTLYF
360 370 380 390 400
DDRKLITGSL DKTIRVWNYI TGECISTYRG HSDSVLSVDS YQKVIVSGSA
410 420 430 440 450
DKTVKVWHVE SRTCYTLRGH TEWVNCVKLH PKSFSCFSCS DDTTIRMWDI
460 470 480 490 500
RTNSCLKVFR GHVGQVQKII PLTIKDVENL ATDNTSDGSS PQDDPTMTDG
510 520 530 540 550
ADESDTPSNE QETVLDENIP YPTHLLSCGL DNTIKLWDVK TGKCIRTQFG
560 570 580 590 600
HVEGVWDIAA DNFRIISGSH DGSIKVWDLQ SGKCMHTFNG RRLQRETQHT
610 620 630 640
QTQSLGDKVA PIACVCIGDS ECFSGDEFGC VKMYKFDLND
Length:640
Mass (Da):72,835
Last modified:February 1, 1995 - v1
Checksum:i5135D4BCA2E1EB97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61M → I in AAA96717 (PubMed:8524217).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46861 Genomic DNA. Translation: CAA86905.1.
L26505 Genomic DNA. Translation: AAA96717.1.
BK006942 Genomic DNA. Translation: DAA08502.1.
PIRiS49932.
RefSeqiNP_012218.1. NM_001179396.1.

Genome annotation databases

EnsemblFungiiYIL046W; YIL046W; YIL046W.
GeneIDi854765.
KEGGisce:YIL046W.

Similar proteinsi

Entry informationi

Entry nameiMET30_YEAST
AccessioniPrimary (citable) accession number: P39014
Secondary accession number(s): D6VVN6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 22, 2017
This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names