P39014 (MET30_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box protein MET30

Alternative name(s):
E3 ubiquitin ligase complex SCF(Met30) subunit MET30
Methionine-requiring protein 30

Gene names

Name:	MET30
Ordered Locus Names:	YIL046W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negatively regulates sulfur amino acids biosynthesis genes expression. Controls cell cycle function (being required for the G1/S transition and M-phase but not the S-phase), sulfur metabolism, and methionine biosynthesis as part of the E3 ubiquitin ligase complex SCF(Met30). Required for the efficient binding of CDC45 and MCM proteins to origins of replication. Involved in the S-adenosylmethionine (AdoMet)-mediated inhibition of the transcription function of MET4. In the context of the E3 ubiquitin ligase complex SCF(Met30), involved in the degradation of MET4 and the cellular response to cadmium. Required for efficient expression of G1 cyclins. Ref.1 Ref.5 Ref.9 Ref.10 Ref.11
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Homomultimer. Interacts with MET4. Interacts with CDC53 and SKP1/CBF3D to form the E3 ubiquitin ligase complex SCF(Met30). Ref.1 Ref.4 Ref.5 Ref.9 Ref.12
Subcellular location	Cytoplasm. Nucleus Ref.5 Ref.7 Ref.9.
Induction	Transcriptional activation requires MET4 as well as MET31 and MET32. Regulated by intracellular AdoMet levels. L-methionine regulates the abundance of MET30. The amount of MET30 regulates the activity of the E3 ubiquitin ligase complex SCF(Met30). Ref.5 Ref.6
Miscellaneous	Present with 217 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the WD repeat MET30/SCONB/SCON-2 family. Contains 1 F-box domain. Contains 8 WD repeats.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cell cycle Cysteine biosynthesis Methionine biosynthesis Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from mutant phenotype Ref.11. Source: SGD M phase of mitotic cell cycle Inferred from mutant phenotype Ref.11. Source: SGD SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from genetic interaction PubMed 9716410. Source: SGD cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW protein polyubiquitination Inferred from mutant phenotype PubMed 9716410. Source: SGD regulation of DNA-dependent DNA replication initiation Inferred from mutant phenotype Ref.11. Source: SGD regulation of transcription involved in G1 phase of mitotic cell cycle Inferred from mutant phenotype Ref.11. Source: SGD response to arsenic-containing substance Inferred from direct assay Ref.10. Source: SGD response to cadmium ion Inferred from direct assay Ref.10. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear SCF ubiquitin ligase complex Inferred from direct assay Ref.5. Source: SGD
Molecular_function	ubiquitin binding Inferred from direct assay PubMed 21070969. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-11507,EBI-11507
CDC53	Q12018	13	EBI-11507,EBI-4321
MET4	P32389	8	EBI-11507,EBI-10757
SKP1	P52286	14	EBI-11507,EBI-4090
SRL2	Q12020	3	EBI-11507,EBI-38714

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 640	640	F-box protein MET30		PRO_0000051087
Regions
	Domain	181 – 227	47	F-box
	Repeat	300 – 328	29	WD 1
	Repeat	340 – 368	29	WD 2
	Repeat	380 – 408	29	WD 3
	Repeat	419 – 449	31	WD 4
	Repeat	461 – 499	39	WD 5
	Repeat	509 – 538	30	WD 6
	Repeat	550 – 578	29	WD 7
	Repeat	607 – 635	29	WD 8
	Region	1 – 299	299	Necessary to mediate nuclear localization
	Region	180 – 277	98	Important for mediating homomultimerization
	Region	180 – 225	46	Interaction with SKP1/CBF3D
	Region	277 – 640	364	Interaction with MET4
Amino acid modifications
	Modified residue	47	1	Phosphoserine Ref.13
	Modified residue	48	1	Phosphoserine Ref.13
	Modified residue	69	1	Phosphoserine Ref.13
	Modified residue	105	1	Phosphoserine Ref.13
Experimental info
	Mutagenesis	187	1	L → D: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. Ref.9
	Mutagenesis	190	1	E → A: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. Ref.9
	Mutagenesis	386	1	L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425 and A-467. Ref.12
	Mutagenesis	425	1	N → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-530 or D-386 and A-467. Ref.12
	Mutagenesis	467	1	Q → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-386 and A-425. Ref.12
	Mutagenesis	530	1	L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425. Ref.12
	Sequence conflict	61	1	M → I in AAA96717. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39014 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 5135D4BCA2E1EB97	FASTA	640	72,835
	10 20 30 40 50 60 MRRERQRMMS FEDKDKDDLD NSNSNNSSEM TDTAMMPPLK RLLITGSSDD LAQGSSGKKK 70 80 90 100 110 120 MTMATRSPSS SPDLATNDSG TRVQPLPEYN FTKFCYRHNP DIQFSPTHTA CYKQDLKRTQ 130 140 150 160 170 180 EINANIAKLP LQEQSDIHHI ISKYSNSNDK IRKLILDGIL STSCFPQLSY ISSLVTHMIK 190 200 210 220 230 240 IDFISILPQE LSLKILSYLD CQSLCNATRV CRKWQKLADD DRVWYHMCEQ HIDRKCPNCG 250 260 270 280 290 300 WGLPLLHMKR ARIQQNSTGS SSNADIQTQT TRPWKVIYRE RFKVESNWRK GHCRIQEFKG 310 320 330 340 350 360 HMDGVLTLQF NYRLLFTGSY DSTIGIWDLF TGKLIRRLSG HSDGVKTLYF DDRKLITGSL 370 380 390 400 410 420 DKTIRVWNYI TGECISTYRG HSDSVLSVDS YQKVIVSGSA DKTVKVWHVE SRTCYTLRGH 430 440 450 460 470 480 TEWVNCVKLH PKSFSCFSCS DDTTIRMWDI RTNSCLKVFR GHVGQVQKII PLTIKDVENL 490 500 510 520 530 540 ATDNTSDGSS PQDDPTMTDG ADESDTPSNE QETVLDENIP YPTHLLSCGL DNTIKLWDVK 550 560 570 580 590 600 TGKCIRTQFG HVEGVWDIAA DNFRIISGSH DGSIKVWDLQ SGKCMHTFNG RRLQRETQHT 610 620 630 640 QTQSLGDKVA PIACVCIGDS ECFSGDEFGC VKMYKFDLND

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats." Thomas D., Kuras L., Barbey R., Cherest H., Blaiseau P.L., Surdin-Kerjan Y. Mol. Cell. Biol. 15:6526-6534(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH MET4. Strain: ATCC 26786 / X2180-1A.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast." Patton E.E., Willems A.R., Sa D., Kuras L., Thomas D., Craig K.L., Tyers M. Genes Dev. 12:692-705(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SKP1/CBF3D AND CDC53.
[5]	"Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30) complex." Rouillon A., Barbey R., Patton E.E., Tyers M., Thomas D. EMBO J. 19:282-294(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH MET4 AND SKP1/CBF3D.
[6]	"The abundance of Met30p limits SCF(Met30p) complex activity and is regulated by methionine availability." Smothers D.B., Kozubowski L., Dixon C., Goebl M.G., Mathias N. Mol. Cell. Biol. 20:7845-7852(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The amino-terminal portion of the F-box protein Met30p mediates its nuclear import and assimilation into an SCF complex." Brunson L.E., Dixon C., Kozubowski L., Mathias N. J. Biol. Chem. 279:6674-6682(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SKP1/CBF3D, HOMOMULTIMERIZATION, MUTAGENESIS OF LEU-187 AND GLU-190.
[10]	"The yeast ubiquitin ligase SCFMet30 regulates heavy metal response." Yen J.L., Su N.Y., Kaiser P. Mol. Biol. Cell 16:1872-1882(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"The F-box protein Met30 is required for multiple steps in the budding yeast cell cycle." Su N.Y., Flick K., Kaiser P. Mol. Cell. Biol. 25:3875-3885(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p." Brunson L.E., Dixon C., LeFebvre A., Sun L., Mathias N. Mol. Genet. Genomics 273:361-370(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MET4, MUTAGENESIS OF LEU-386; ASN-425; GLN-467 AND LEU-530.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; SER-48; SER-69 AND SER-105, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46861 Genomic DNA. Translation: CAA86905.1. L26505 Genomic DNA. Translation: AAA96717.1. BK006942 Genomic DNA. Translation: DAA08502.1.
PIR	S49932.
RefSeq	NP_012218.1. NM_001179396.1.
3D structure databases
ProteinModelPortal	P39014.
SMR	P39014. Positions 167-635.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1439N.
IntAct	P39014. 79 interactions.
MINT	MINT-385167.
STRING	4932.YIL046W.
Proteomic databases
PaxDb	P39014.
PeptideAtlas	P39014.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL046W; YIL046W; YIL046W.
GeneID	854765.
KEGG	sce:YIL046W.
Organism-specific databases
CYGD	YIL046w.
SGD	S000001308. MET30.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00690000101849.
HOGENOM	HOG000166480.
KO	K10259.
OMA	ILCYLDT.
OrthoDB	EOG496313.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	P39014.
GermOnline	YIL046W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 3 hits.
InterPro	IPR001810. F-box_dom_cyclin-like. IPR020472. G-protein_beta_WD-40_rep. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00646. F-box. 1 hit. PF00400. WD40. 6 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00256. FBOX. 1 hit. SM00320. WD40. 6 hits. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit. SSF50978. WD40_like. 1 hit.
PROSITE	PS50181. FBOX. 1 hit. PS00678. WD_REPEATS_1. 4 hits. PS50082. WD_REPEATS_2. 6 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977517.

Entry information

Entry name

MET30_YEAST

Accession

Primary (citable) accession number: P39014
Secondary accession number(s): D6VVN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program