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Protein

F-box protein MET30

Gene

MET30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates sulfur amino acids biosynthesis genes expression. Controls cell cycle function (being required for the G1/S transition and M-phase but not the S-phase), sulfur metabolism, and methionine biosynthesis as part of the E3 ubiquitin ligase complex SCF(Met30). Required for the efficient binding of CDC45 and MCM proteins to origins of replication. Involved in the S-adenosylmethionine (AdoMet)-mediated inhibition of the transcription function of MET4. In the context of the E3 ubiquitin ligase complex SCF(Met30), involved in the degradation of MET4 and the cellular response to cadmium. Required for efficient expression of G1 cyclins.5 Publications

Pathway: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • ubiquitin binding Source: SGD

GO - Biological processi

  • cysteine biosynthetic process Source: UniProtKB-KW
  • DNA replication initiation Source: SGD
  • G1/S transition of mitotic cell cycle Source: SGD
  • methionine biosynthetic process Source: UniProtKB-KW
  • protein polyubiquitination Source: SGD
  • protein ubiquitination Source: SGD
  • regulation of DNA-dependent DNA replication initiation Source: SGD
  • regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
  • response to arsenic-containing substance Source: SGD
  • response to cadmium ion Source: SGD
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Amino-acid biosynthesis, Cell cycle, Cysteine biosynthesis, Methionine biosynthesis, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31317-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box protein MET30
Alternative name(s):
E3 ubiquitin ligase complex SCF(Met30) subunit MET30
Methionine-requiring protein 30
Gene namesi
Name:MET30
Ordered Locus Names:YIL046W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IX

Organism-specific databases

CYGDiYIL046w.
EuPathDBiFungiDB:YIL046W.
SGDiS000001308. MET30.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear SCF ubiquitin ligase complex Source: SGD
  • nucleus Source: SGD
  • SCF ubiquitin ligase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi187 – 1871L → D: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. 1 Publication
Mutagenesisi190 – 1901E → A: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. 1 Publication
Mutagenesisi386 – 3861L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425 and A-467. 1 Publication
Mutagenesisi425 – 4251N → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-530 or D-386 and A-467. 1 Publication
Mutagenesisi467 – 4671Q → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-386 and A-425. 1 Publication
Mutagenesisi530 – 5301L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 640640F-box protein MET30PRO_0000051087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39014.
PaxDbiP39014.
PeptideAtlasiP39014.

Expressioni

Inductioni

Transcriptional activation requires MET4 as well as MET31 and MET32. Regulated by intracellular AdoMet levels. L-methionine regulates the abundance of MET30. The amount of MET30 regulates the activity of the E3 ubiquitin ligase complex SCF(Met30).2 Publications

Interactioni

Subunit structurei

Homomultimer. Interacts with MET4. Interacts with CDC53 and SKP1/CBF3D to form the E3 ubiquitin ligase complex SCF(Met30).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-11507,EBI-11507
CDC53Q120189EBI-11507,EBI-4321
MET4P323896EBI-11507,EBI-10757
SKP1P5228613EBI-11507,EBI-4090
SRL2Q120203EBI-11507,EBI-38714

Protein-protein interaction databases

BioGridi34944. 108 interactions.
DIPiDIP-1439N.
IntActiP39014. 74 interactions.
MINTiMINT-385167.
STRINGi4932.YIL046W.

Structurei

3D structure databases

ProteinModelPortaliP39014.
SMRiP39014. Positions 167-636.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini181 – 22747F-boxPROSITE-ProRule annotationAdd
BLAST
Repeati300 – 32829WD 1Add
BLAST
Repeati340 – 36829WD 2Add
BLAST
Repeati380 – 40829WD 3Add
BLAST
Repeati419 – 44931WD 4Add
BLAST
Repeati461 – 49939WD 5Add
BLAST
Repeati509 – 53830WD 6Add
BLAST
Repeati550 – 57829WD 7Add
BLAST
Repeati607 – 63529WD 8Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 299299Necessary to mediate nuclear localizationAdd
BLAST
Regioni180 – 27798Important for mediating homomultimerizationAdd
BLAST
Regioni180 – 22546Interaction with SKP1/CBF3DAdd
BLAST
Regioni277 – 640364Interaction with MET4Add
BLAST

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119106.
HOGENOMiHOG000166480.
InParanoidiP39014.
KOiK10259.
OMAiYHTGECI.
OrthoDBiEOG7C5MHX.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00646. F-box. 1 hit.
PF12937. F-box-like. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00256. FBOX. 1 hit.
SM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRERQRMMS FEDKDKDDLD NSNSNNSSEM TDTAMMPPLK RLLITGSSDD
60 70 80 90 100
LAQGSSGKKK MTMATRSPSS SPDLATNDSG TRVQPLPEYN FTKFCYRHNP
110 120 130 140 150
DIQFSPTHTA CYKQDLKRTQ EINANIAKLP LQEQSDIHHI ISKYSNSNDK
160 170 180 190 200
IRKLILDGIL STSCFPQLSY ISSLVTHMIK IDFISILPQE LSLKILSYLD
210 220 230 240 250
CQSLCNATRV CRKWQKLADD DRVWYHMCEQ HIDRKCPNCG WGLPLLHMKR
260 270 280 290 300
ARIQQNSTGS SSNADIQTQT TRPWKVIYRE RFKVESNWRK GHCRIQEFKG
310 320 330 340 350
HMDGVLTLQF NYRLLFTGSY DSTIGIWDLF TGKLIRRLSG HSDGVKTLYF
360 370 380 390 400
DDRKLITGSL DKTIRVWNYI TGECISTYRG HSDSVLSVDS YQKVIVSGSA
410 420 430 440 450
DKTVKVWHVE SRTCYTLRGH TEWVNCVKLH PKSFSCFSCS DDTTIRMWDI
460 470 480 490 500
RTNSCLKVFR GHVGQVQKII PLTIKDVENL ATDNTSDGSS PQDDPTMTDG
510 520 530 540 550
ADESDTPSNE QETVLDENIP YPTHLLSCGL DNTIKLWDVK TGKCIRTQFG
560 570 580 590 600
HVEGVWDIAA DNFRIISGSH DGSIKVWDLQ SGKCMHTFNG RRLQRETQHT
610 620 630 640
QTQSLGDKVA PIACVCIGDS ECFSGDEFGC VKMYKFDLND
Length:640
Mass (Da):72,835
Last modified:February 1, 1995 - v1
Checksum:i5135D4BCA2E1EB97
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611M → I in AAA96717 (PubMed:8524217).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46861 Genomic DNA. Translation: CAA86905.1.
L26505 Genomic DNA. Translation: AAA96717.1.
BK006942 Genomic DNA. Translation: DAA08502.1.
PIRiS49932.
RefSeqiNP_012218.1. NM_001179396.1.

Genome annotation databases

EnsemblFungiiYIL046W; YIL046W; YIL046W.
GeneIDi854765.
KEGGisce:YIL046W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46861 Genomic DNA. Translation: CAA86905.1.
L26505 Genomic DNA. Translation: AAA96717.1.
BK006942 Genomic DNA. Translation: DAA08502.1.
PIRiS49932.
RefSeqiNP_012218.1. NM_001179396.1.

3D structure databases

ProteinModelPortaliP39014.
SMRiP39014. Positions 167-636.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34944. 108 interactions.
DIPiDIP-1439N.
IntActiP39014. 74 interactions.
MINTiMINT-385167.
STRINGi4932.YIL046W.

Proteomic databases

MaxQBiP39014.
PaxDbiP39014.
PeptideAtlasiP39014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL046W; YIL046W; YIL046W.
GeneIDi854765.
KEGGisce:YIL046W.

Organism-specific databases

CYGDiYIL046w.
EuPathDBiFungiDB:YIL046W.
SGDiS000001308. MET30.

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119106.
HOGENOMiHOG000166480.
InParanoidiP39014.
KOiK10259.
OMAiYHTGECI.
OrthoDBiEOG7C5MHX.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-31317-MONOMER.

Miscellaneous databases

NextBioi977517.
PROiP39014.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00646. F-box. 1 hit.
PF12937. F-box-like. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00256. FBOX. 1 hit.
SM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats."
    Thomas D., Kuras L., Barbey R., Cherest H., Blaiseau P.L., Surdin-Kerjan Y.
    Mol. Cell. Biol. 15:6526-6534(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH MET4.
    Strain: ATCC 26786 / X2180-1A.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast."
    Patton E.E., Willems A.R., Sa D., Kuras L., Thomas D., Craig K.L., Tyers M.
    Genes Dev. 12:692-705(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKP1/CBF3D AND CDC53.
  5. "Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30) complex."
    Rouillon A., Barbey R., Patton E.E., Tyers M., Thomas D.
    EMBO J. 19:282-294(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH MET4 AND SKP1/CBF3D.
  6. "The abundance of Met30p limits SCF(Met30p) complex activity and is regulated by methionine availability."
    Smothers D.B., Kozubowski L., Dixon C., Goebl M.G., Mathias N.
    Mol. Cell. Biol. 20:7845-7852(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "The amino-terminal portion of the F-box protein Met30p mediates its nuclear import and assimilation into an SCF complex."
    Brunson L.E., Dixon C., Kozubowski L., Mathias N.
    J. Biol. Chem. 279:6674-6682(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SKP1/CBF3D, HOMOMULTIMERIZATION, MUTAGENESIS OF LEU-187 AND GLU-190.
  10. "The yeast ubiquitin ligase SCFMet30 regulates heavy metal response."
    Yen J.L., Su N.Y., Kaiser P.
    Mol. Biol. Cell 16:1872-1882(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "The F-box protein Met30 is required for multiple steps in the budding yeast cell cycle."
    Su N.Y., Flick K., Kaiser P.
    Mol. Cell. Biol. 25:3875-3885(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p."
    Brunson L.E., Dixon C., LeFebvre A., Sun L., Mathias N.
    Mol. Genet. Genomics 273:361-370(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MET4, MUTAGENESIS OF LEU-386; ASN-425; GLN-467 AND LEU-530.
  13. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMET30_YEAST
AccessioniPrimary (citable) accession number: P39014
Secondary accession number(s): D6VVN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 24, 2015
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.