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P39014 (MET30_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
F-box protein MET30
Alternative name(s):
E3 ubiquitin ligase complex SCF(Met30) subunit MET30
Methionine-requiring protein 30
Gene names
Name:MET30
Ordered Locus Names:YIL046W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negatively regulates sulfur amino acids biosynthesis genes expression. Controls cell cycle function (being required for the G1/S transition and M-phase but not the S-phase), sulfur metabolism, and methionine biosynthesis as part of the E3 ubiquitin ligase complex SCF(Met30). Required for the efficient binding of CDC45 and MCM proteins to origins of replication. Involved in the S-adenosylmethionine (AdoMet)-mediated inhibition of the transcription function of MET4. In the context of the E3 ubiquitin ligase complex SCF(Met30), involved in the degradation of MET4 and the cellular response to cadmium. Required for efficient expression of G1 cyclins. Ref.1 Ref.5 Ref.9 Ref.10 Ref.11

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Homomultimer. Interacts with MET4. Interacts with CDC53 and SKP1/CBF3D to form the E3 ubiquitin ligase complex SCF(Met30). Ref.1 Ref.4 Ref.5 Ref.9 Ref.12

Subcellular location

Cytoplasm. Nucleus Ref.5 Ref.7 Ref.9.

Induction

Transcriptional activation requires MET4 as well as MET31 and MET32. Regulated by intracellular AdoMet levels. L-methionine regulates the abundance of MET30. The amount of MET30 regulates the activity of the E3 ubiquitin ligase complex SCF(Met30). Ref.5 Ref.6

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the WD repeat MET30/SCONB/SCON-2 family.

Contains 1 F-box domain.

Contains 8 WD repeats.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Cell cycle
Cysteine biosynthesis
Methionine biosynthesis
Transcription
Transcription regulation
Ubl conjugation pathway
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
WD repeat
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication initiation

Inferred from mutant phenotype Ref.11. Source: SGD

G1/S transition of mitotic cell cycle

Inferred from mutant phenotype Ref.11. Source: SGD

SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Inferred from genetic interaction PubMed 9716410. Source: SGD

cysteine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

methionine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

protein polyubiquitination

Inferred from mutant phenotype PubMed 9716410. Source: SGD

protein ubiquitination

Inferred from genetic interaction Ref.4. Source: SGD

regulation of DNA-dependent DNA replication initiation

Inferred from mutant phenotype Ref.11. Source: SGD

regulation of transcription involved in G1/S transition of mitotic cell cycle

Inferred from mutant phenotype Ref.11. Source: SGD

response to arsenic-containing substance

Inferred from direct assay Ref.10. Source: SGD

response to cadmium ion

Inferred from direct assay Ref.10. Source: SGD

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentSCF ubiquitin ligase complex

Inferred from direct assay PubMed 9716410. Source: SGD

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nuclear SCF ubiquitin ligase complex

Inferred from direct assay Ref.5. Source: SGD

nucleus

Inferred from direct assay Ref.5. Source: SGD

   Molecular_functionidentical protein binding

Inferred from physical interaction Ref.9. Source: IntAct

ubiquitin binding

Inferred from direct assay PubMed 21070969. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 640640F-box protein MET30
PRO_0000051087

Regions

Domain181 – 22747F-box
Repeat300 – 32829WD 1
Repeat340 – 36829WD 2
Repeat380 – 40829WD 3
Repeat419 – 44931WD 4
Repeat461 – 49939WD 5
Repeat509 – 53830WD 6
Repeat550 – 57829WD 7
Repeat607 – 63529WD 8
Region1 – 299299Necessary to mediate nuclear localization
Region180 – 27798Important for mediating homomultimerization
Region180 – 22546Interaction with SKP1/CBF3D
Region277 – 640364Interaction with MET4

Amino acid modifications

Modified residue671Phosphoserine Ref.15

Experimental info

Mutagenesis1871L → D: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. Ref.9
Mutagenesis1901E → A: Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D. Ref.9
Mutagenesis3861L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425 and A-467. Ref.12
Mutagenesis4251N → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-530 or D-386 and A-467. Ref.12
Mutagenesis4671Q → A: Inactivates MET30 and prevents MET4 interaction; when associated with D-386 and A-425. Ref.12
Mutagenesis5301L → D: Inactivates MET30 and prevents MET4 interaction; when associated with A-425. Ref.12
Sequence conflict611M → I in AAA96717. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39014 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 5135D4BCA2E1EB97

FASTA64072,835
        10         20         30         40         50         60 
MRRERQRMMS FEDKDKDDLD NSNSNNSSEM TDTAMMPPLK RLLITGSSDD LAQGSSGKKK 

        70         80         90        100        110        120 
MTMATRSPSS SPDLATNDSG TRVQPLPEYN FTKFCYRHNP DIQFSPTHTA CYKQDLKRTQ 

       130        140        150        160        170        180 
EINANIAKLP LQEQSDIHHI ISKYSNSNDK IRKLILDGIL STSCFPQLSY ISSLVTHMIK 

       190        200        210        220        230        240 
IDFISILPQE LSLKILSYLD CQSLCNATRV CRKWQKLADD DRVWYHMCEQ HIDRKCPNCG 

       250        260        270        280        290        300 
WGLPLLHMKR ARIQQNSTGS SSNADIQTQT TRPWKVIYRE RFKVESNWRK GHCRIQEFKG 

       310        320        330        340        350        360 
HMDGVLTLQF NYRLLFTGSY DSTIGIWDLF TGKLIRRLSG HSDGVKTLYF DDRKLITGSL 

       370        380        390        400        410        420 
DKTIRVWNYI TGECISTYRG HSDSVLSVDS YQKVIVSGSA DKTVKVWHVE SRTCYTLRGH 

       430        440        450        460        470        480 
TEWVNCVKLH PKSFSCFSCS DDTTIRMWDI RTNSCLKVFR GHVGQVQKII PLTIKDVENL 

       490        500        510        520        530        540 
ATDNTSDGSS PQDDPTMTDG ADESDTPSNE QETVLDENIP YPTHLLSCGL DNTIKLWDVK 

       550        560        570        580        590        600 
TGKCIRTQFG HVEGVWDIAA DNFRIISGSH DGSIKVWDLQ SGKCMHTFNG RRLQRETQHT 

       610        620        630        640 
QTQSLGDKVA PIACVCIGDS ECFSGDEFGC VKMYKFDLND 

« Hide

References

« Hide 'large scale' references
[1]"Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats."
Thomas D., Kuras L., Barbey R., Cherest H., Blaiseau P.L., Surdin-Kerjan Y.
Mol. Cell. Biol. 15:6526-6534(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH MET4.
Strain: ATCC 26786 / X2180-1A.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast."
Patton E.E., Willems A.R., Sa D., Kuras L., Thomas D., Craig K.L., Tyers M.
Genes Dev. 12:692-705(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKP1/CBF3D AND CDC53.
[5]"Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30) complex."
Rouillon A., Barbey R., Patton E.E., Tyers M., Thomas D.
EMBO J. 19:282-294(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH MET4 AND SKP1/CBF3D.
[6]"The abundance of Met30p limits SCF(Met30p) complex activity and is regulated by methionine availability."
Smothers D.B., Kozubowski L., Dixon C., Goebl M.G., Mathias N.
Mol. Cell. Biol. 20:7845-7852(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"The amino-terminal portion of the F-box protein Met30p mediates its nuclear import and assimilation into an SCF complex."
Brunson L.E., Dixon C., Kozubowski L., Mathias N.
J. Biol. Chem. 279:6674-6682(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SKP1/CBF3D, HOMOMULTIMERIZATION, MUTAGENESIS OF LEU-187 AND GLU-190.
[10]"The yeast ubiquitin ligase SCFMet30 regulates heavy metal response."
Yen J.L., Su N.Y., Kaiser P.
Mol. Biol. Cell 16:1872-1882(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"The F-box protein Met30 is required for multiple steps in the budding yeast cell cycle."
Su N.Y., Flick K., Kaiser P.
Mol. Cell. Biol. 25:3875-3885(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p."
Brunson L.E., Dixon C., LeFebvre A., Sun L., Mathias N.
Mol. Genet. Genomics 273:361-370(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MET4, MUTAGENESIS OF LEU-386; ASN-425; GLN-467 AND LEU-530.
[13]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[14]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46861 Genomic DNA. Translation: CAA86905.1.
L26505 Genomic DNA. Translation: AAA96717.1.
BK006942 Genomic DNA. Translation: DAA08502.1.
PIRS49932.
RefSeqNP_012218.1. NM_001179396.1.

3D structure databases

ProteinModelPortalP39014.
SMRP39014. Positions 166-639.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34944. 106 interactions.
DIPDIP-1439N.
IntActP39014. 74 interactions.
MINTMINT-385167.
STRING4932.YIL046W.

Proteomic databases

PaxDbP39014.
PeptideAtlasP39014.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL046W; YIL046W; YIL046W.
GeneID854765.
KEGGsce:YIL046W.

Organism-specific databases

CYGDYIL046w.
SGDS000001308. MET30.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00710000106459.
HOGENOMHOG000166480.
KOK10259.
OMAYHTGECI.
OrthoDBEOG7C5MHX.

Enzyme and pathway databases

BioCycYEAST:G3O-31317-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorP39014.

Family and domain databases

Gene3D2.130.10.10. 3 hits.
InterProIPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00646. F-box. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00256. FBOX. 1 hit.
SM00320. WD40. 6 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 2 hits.
SSF81383. SSF81383. 1 hit.
PROSITEPS50181. FBOX. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977517.

Entry information

Entry nameMET30_YEAST
AccessionPrimary (citable) accession number: P39014
Secondary accession number(s): D6VVN6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways