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Protein

Palmitoyltransferase AKR1

Gene

AKR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Palmitoyltransferase specific for casein kinase 1. Palmitoylates isoforms YCK1 and YCK2 at both C-terminal cysteine residues, which is required for their proper plasma membrane localization. Required for constitutive endocytosis of a-factor receptor STE3 and both constitutive and pheromone-induced endocytosis of alpha-factor receptor STE2.5 Publications

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei500S-palmitoyl cysteine intermediate1 Publication1

GO - Molecular functioni

GO - Biological processi

  • protein palmitoylation Source: UniProtKB
  • protein targeting to membrane Source: SGD
  • regulation of endocytosis Source: SGD
  • regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-29834-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase AKR1 (EC:2.3.1.225)
Alternative name(s):
Ankyrin repeat-containing protein AKR1
Gene namesi
Name:AKR1
Ordered Locus Names:YDR264C
ORF Names:D9954.9, YD9230B.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR264C.
SGDiS000002672. AKR1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 321CytoplasmicAdd BLAST321
Transmembranei322 – 341HelicalSequence analysisAdd BLAST20
Topological domaini342 – 346Lumenal5
Transmembranei347 – 364HelicalSequence analysisAdd BLAST18
Topological domaini365 – 384CytoplasmicAdd BLAST20
Transmembranei385 – 405HelicalSequence analysisAdd BLAST21
Topological domaini406 – 418LumenalAdd BLAST13
Transmembranei419 – 439HelicalSequence analysisAdd BLAST21
Topological domaini440 – 513CytoplasmicAdd BLAST74
Transmembranei514 – 534HelicalSequence analysisAdd BLAST21
Topological domaini535 – 570LumenalAdd BLAST36
Transmembranei571 – 591HelicalSequence analysisAdd BLAST21
Topological domaini592 – 764CytoplasmicAdd BLAST173

GO - Cellular componenti

  • early endosome membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi497 – 498DH → AA: Abolishes YCK2 palmitoylation. 1 Publication2
Mutagenesisi500C → A: Abolishes YCK2 palmitoylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002129341 – 764Palmitoyltransferase AKR1Add BLAST764

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51PhosphoserineCombined sources1
Modified residuei57PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP39010.
PRIDEiP39010.

PTM databases

iPTMnetiP39010.
SwissPalmiP39010.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
STE4P188513EBI-2421,EBI-7390
YCK1P232913EBI-2421,EBI-4718

Protein-protein interaction databases

BioGridi32320. 142 interactors.
DIPiDIP-1145N.
IntActiP39010. 39 interactors.
MINTiMINT-544657.

Structurei

3D structure databases

ProteinModelPortaliP39010.
SMRiP39010.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati72 – 102ANK 1Add BLAST31
Repeati108 – 137ANK 2Add BLAST30
Repeati142 – 171ANK 3Add BLAST30
Repeati175 – 204ANK 4Add BLAST30
Repeati213 – 242ANK 5Add BLAST30
Repeati246 – 275ANK 6Add BLAST30
Domaini470 – 520DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 DHHC domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063074.
HOGENOMiHOG000033883.
InParanoidiP39010.
KOiK20032.
OMAiRWGANVN.
OrthoDBiEOG092C216R.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF01529. zf-DHHC. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS50216. DHHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNELENVPR ASTLTNEEQT VDPSNNDSQE DISLGDSNEI TSLASLKAIR
60 70 80 90 100
SGNEEESGNE QVNHNDEAEE DPLLTRYHTA CQRGDLATVK EMIHGKLLEV
110 120 130 140 150
NNDGDSTEHI TGLHWASINN RLSVVDFLVS QGADVNARAG ALHATPLHWA
160 170 180 190 200
ARYGYVYIVD FLLKHGADPT MTDDQGFNLL HLSVNSSNIM LVLYVLFNVV
210 220 230 240 250
SKGLLDIDCR DPKGRTSLLW AAYQGDSLTV AELLKFGASI KIADTEGFTP
260 270 280 290 300
LHWGTVKGQP HVLKYLIQDG ADFFQKTDTG KDCFAIAQEM NTVYSLREAL
310 320 330 340 350
THSGFDYHGY PIKKWFKKSQ HAKLVTFITP FLFLGIAFAL FSHINPLFVI
360 370 380 390 400
IVLFLLAIAT NKGLNKFVLP SYGRMGVHNV TLLRSPLLSG VFFGTLLWVT
410 420 430 440 450
IVWFFKVMPR TFSDEQYTNI LMLVILVSVF YLFGQLVIMD PGCLPEETDH
460 470 480 490 500
ENVRQTISNL LEIGKFDTKN FCIETWIRKP LRSKFSPLNN AVVARFDHYC
510 520 530 540 550
PWIFNDVGLK NHKAFIFFIT LMESGIFTFL ALCLEYFDEL EDAHEDTSQK
560 570 580 590 600
NGKCFILGAS DLCSGLIYDR FVFLILLWAL LQSIWVASLI FVQAFQICKG
610 620 630 640 650
MTNTEFNVLM KESKSIGPDG LSFNENFNTT PEGFAPSIDP GEESNDTVLA
660 670 680 690 700
PVPGSTIRKP RTCFGVCYAV TGMDQWLAVI KETIGIKDST GHNVYSITSR
710 720 730 740 750
IPTNYGWKRN VKDFWLTSDI NAPLWRRILY PPSGSKALLN GIEVDYFKLY
760
KLPNKDVEQG NDMV
Length:764
Mass (Da):85,840
Last modified:February 1, 1995 - v1
Checksum:i9C9759F0140CE3F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31407 Genomic DNA. Translation: AAC41676.1.
U51030 Genomic DNA. Translation: AAB64454.1.
Z70202 Genomic DNA. Translation: CAA94103.1.
Z68290 Genomic DNA. Translation: CAA92582.1.
BK006938 Genomic DNA. Translation: DAA12108.1.
PIRiS48521.
RefSeqiNP_010550.1. NM_001180572.1.

Genome annotation databases

EnsemblFungiiYDR264C; YDR264C; YDR264C.
GeneIDi851857.
KEGGisce:YDR264C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31407 Genomic DNA. Translation: AAC41676.1.
U51030 Genomic DNA. Translation: AAB64454.1.
Z70202 Genomic DNA. Translation: CAA94103.1.
Z68290 Genomic DNA. Translation: CAA92582.1.
BK006938 Genomic DNA. Translation: DAA12108.1.
PIRiS48521.
RefSeqiNP_010550.1. NM_001180572.1.

3D structure databases

ProteinModelPortaliP39010.
SMRiP39010.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32320. 142 interactors.
DIPiDIP-1145N.
IntActiP39010. 39 interactors.
MINTiMINT-544657.

PTM databases

iPTMnetiP39010.
SwissPalmiP39010.

Proteomic databases

MaxQBiP39010.
PRIDEiP39010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR264C; YDR264C; YDR264C.
GeneIDi851857.
KEGGisce:YDR264C.

Organism-specific databases

EuPathDBiFungiDB:YDR264C.
SGDiS000002672. AKR1.

Phylogenomic databases

GeneTreeiENSGT00530000063074.
HOGENOMiHOG000033883.
InParanoidiP39010.
KOiK20032.
OMAiRWGANVN.
OrthoDBiEOG092C216R.

Enzyme and pathway databases

BioCyciYEAST:G3O-29834-MONOMER.

Miscellaneous databases

PROiP39010.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001594. Znf_DHHC_palmitoyltrfase.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF01529. zf-DHHC. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS50216. DHHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKR1_YEAST
AccessioniPrimary (citable) accession number: P39010
Secondary accession number(s): D6VSP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4072 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.