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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3

Gene

STT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.1 Publication

Miscellaneous

Present with 3000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YGL022W-MONOMER.
YEAST:YGL022W-MONOMER.
BRENDAi2.4.99.18. 984.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit STT3
Gene namesi
Name:STT3
Ordered Locus Names:YGL022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL022W.
SGDiS000002990. STT3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 113LumenalSequence analysisAdd BLAST84
Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
Topological domaini135 – 168CytoplasmicSequence analysisAdd BLAST34
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 207LumenalSequence analysisAdd BLAST18
Transmembranei208 – 228HelicalSequence analysisAdd BLAST21
Topological domaini229 – 240CytoplasmicSequence analysisAdd BLAST12
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
Topological domaini262 – 266LumenalSequence analysis5
Transmembranei267 – 287HelicalSequence analysisAdd BLAST21
Topological domaini288 – 296CytoplasmicSequence analysis9
Transmembranei297 – 316HelicalSequence analysisAdd BLAST20
Topological domaini317 – 358LumenalSequence analysisAdd BLAST42
Transmembranei359 – 379HelicalSequence analysisAdd BLAST21
Topological domaini380 – 385CytoplasmicSequence analysis6
Transmembranei386 – 403HelicalSequence analysisAdd BLAST18
Topological domaini404LumenalSequence analysis1
Transmembranei405 – 425HelicalSequence analysisAdd BLAST21
Topological domaini426 – 443CytoplasmicSequence analysisAdd BLAST18
Transmembranei444 – 464HelicalSequence analysisAdd BLAST21
Topological domaini465 – 718LumenalSequence analysisAdd BLAST254

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi159R → A: Temperature sensitive and staurosporine sensitive. 1 Publication1
Mutagenesisi160S → A: Temperature sensitive and staurosporine sensitive. 1 Publication1
Mutagenesisi163G → R: Temperature sensitive and staurosporine sensitive. 1
Mutagenesisi164S → A: Temperature sensitive and staurosporine sensitive. 1 Publication1
Mutagenesisi208W → A: Lethal; abolishes interaction with OST1 and WBP1. 1 Publication1
Mutagenesisi210G → D: Temperature sensitive and staurosporine sensitive. 1 Publication1
Mutagenesisi393V → I: Staurosporine sensitive. 1 Publication1
Mutagenesisi404R → A: Lethal; abolishes interaction with OST1 and WBP1. 1 Publication1
Mutagenesisi516 – 519WWDY → AAAA: Lethal; greatly reduces amount of OST1 in complex. 4
Mutagenesisi516W → A: Temperature-sensitive. 1 Publication1
Mutagenesisi517W → A: Lethal. 1 Publication1
Mutagenesisi518D → A: Lethal. 1 Publication1
Mutagenesisi519Y → A: Temperature-sensitive. 1 Publication1
Mutagenesisi520G → D: Temperature-sensitive. 1 Publication1
Mutagenesisi554 – 556EEK → AAA: Temperature-sensitive; reduces amount of OST1 in complex. 3
Mutagenesisi592 – 594RIS → AAA: Lethal; reduces glycosylation; greatly reduces amount of OST1 in complex. 3
Mutagenesisi593I → D: Temperature-sensitive. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000722931 – 718Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi535N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi539N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP39007.
PRIDEiP39007.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST3, OST4 and STT3 probably form a subcomplex. May interact directly with OST3, OST4, OST6, WBP1 and SWP1. Interacts with SEC61.5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33224. 638 interactors.
DIPiDIP-2459N.
IntActiP39007. 8 interactors.
MINTiMINT-627098.
STRINGi4932.YGL022W.

Structurei

Secondary structure

1718
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi467 – 470Combined sources4
Helixi472 – 474Combined sources3
Turni481 – 483Combined sources3
Helixi491 – 503Combined sources13
Beta strandi508 – 510Combined sources3
Helixi512 – 521Combined sources10
Turni525 – 527Combined sources3
Turni531 – 535Combined sources5
Helixi536 – 551Combined sources16
Beta strandi552 – 555Combined sources4
Helixi560 – 573Combined sources14
Beta strandi578 – 581Combined sources4
Helixi601 – 606Combined sources6
Beta strandi611 – 616Combined sources6
Helixi621 – 635Combined sources15
Helixi646 – 652Combined sources7
Turni663 – 665Combined sources3
Helixi666 – 670Combined sources5
Helixi675 – 686Combined sources12
Helixi692 – 695Combined sources4
Helixi698 – 700Combined sources3
Beta strandi705 – 708Combined sources4
Beta strandi712 – 716Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LGZNMR-A465-718[»]
ProteinModelPortaliP39007.
SMRiP39007.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the STT3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000015238.
HOGENOMiHOG000157471.
InParanoidiP39007.
KOiK07151.
OMAiSWSSFYF.
OrthoDBiEOG092C0UC6.

Family and domain databases

InterProiView protein in InterPro
IPR003674. Oligo_trans_STT3.
PfamiView protein in Pfam
PF02516. STT3. 1 hit.

Sequencei

Sequence statusi: Complete.

P39007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSDRSCVLS VFQTILKLVI FVAIFGAAIS SRLFAVIKFE SIIHEFDPWF
60 70 80 90 100
NYRATKYLVN NSFYKFLNWF DDRTWYPLGR VTGGTLYPGL MTTSAFIWHA
110 120 130 140 150
LRNWLGLPID IRNVCVLFAP LFSGVTAWAT YEFTKEIKDA SAGLLAAGFI
160 170 180 190 200
AIVPGYISRS VAGSYDNEAI AITLLMVTFM FWIKAQKTGS IMHATCAALF
210 220 230 240 250
YFYMVSAWGG YVFITNLIPL HVFLLILMGR YSSKLYSAYT TWYAIGTVAS
260 270 280 290 300
MQIPFVGFLP IRSNDHMAAL GVFGLIQIVA FGDFVKGQIS TAKFKVIMMV
310 320 330 340 350
SLFLILVLGV VGLSALTYMG LIAPWTGRFY SLWDTNYAKI HIPIIASVSE
360 370 380 390 400
HQPVSWPAFF FDTHFLIWLF PAGVFLLFLD LKDEHVFVIA YSVLCSYFAG
410 420 430 440 450
VMVRLMLTLT PVICVSAAVA LSKIFDIYLD FKTSDRKYAI KPAALLAKLI
460 470 480 490 500
VSGSFIFYLY LFVFHSTWVT RTAYSSPSVV LPSQTPDGKL ALIDDFREAY
510 520 530 540 550
YWLRMNSDED SKVAAWWDYG YQIGGMADRT TLVDNNTWNN THIAIVGKAM
560 570 580 590 600
ASPEEKSYEI LKEHDVDYVL VIFGGLIGFG GDDINKFLWM IRISEGIWPE
610 620 630 640 650
EIKERDFYTA EGEYRVDARA SETMRNSLLY KMSYKDFPQL FNGGQATDRV
660 670 680 690 700
RQQMITPLDV PPLDYFDEVF TSENWMVRIY QLKKDDAQGR TLRDVGELTR
710
SSTKTRRSIK RPELGLRV
Length:718
Mass (Da):81,529
Last modified:October 1, 1996 - v2
Checksum:iD00B8FF7B2B11B9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28952 Genomic DNA. Translation: BAA06079.1.
Z72544 Genomic DNA. Translation: CAA96722.1.
BK006941 Genomic DNA. Translation: DAA08076.1.
PIRiS64024.
RefSeqiNP_011493.1. NM_001180887.1.

Genome annotation databases

EnsemblFungiiYGL022W; YGL022W; YGL022W.
GeneIDi852862.
KEGGisce:YGL022W.

Similar proteinsi

Entry informationi

Entry nameiSTT3_YEAST
AccessioniPrimary (citable) accession number: P39007
Secondary accession number(s): D6VUB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names