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P39002

- LCF3_YEAST

UniProt

P39002 - LCF3_YEAST

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Protein

Long-chain-fatty-acid--CoA ligase 3

Gene
FAA3, YIL009W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Esterification, concomitant with transport, of endogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. This enzyme acts preferentially on C16 and C18 fatty acids with a cis-double bond at C-9-C-10.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Magnesium By similarity.

Temperature dependencei

Optimum temperature is 25 degrees Celsius.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: SGD
  3. very long-chain fatty acid-CoA ligase activity Source: SGD

GO - Biological processi

  1. long-chain fatty acid metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YIL009W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 3 (EC:6.2.1.3)
Alternative name(s):
Fatty acid activator 3
Long-chain acyl-CoA synthetase 3
Gene namesi
Name:FAA3
Ordered Locus Names:YIL009W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL009w.
SGDiS000001271. FAA3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 694693Long-chain-fatty-acid--CoA ligase 3PRO_0000193121Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP39002.
PaxDbiP39002.
PeptideAtlasiP39002.
PRIDEiP39002.

Expressioni

Gene expression databases

GenevestigatoriP39002.

Interactioni

Subunit structurei

Interacts with FRK1.1 Publication

Protein-protein interaction databases

BioGridi34983. 32 interactions.
DIPiDIP-4210N.
IntActiP39002. 8 interactions.
MINTiMINT-562678.
STRINGi4932.YIL009W.

Structurei

3D structure databases

ProteinModelPortaliP39002.
SMRiP39002. Positions 100-683.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1022.
GeneTreeiENSGT00690000102168.
HOGENOMiHOG000159459.
KOiK01897.
OMAiTHENIMA.
OrthoDBiEOG7CCC0K.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39002-1 [UniParc]FASTAAdd to Basket

« Hide

MSEQHSVAVG KAANEHETAP RRNVRVKKRP LIRPLNSSAS TLYEFALECF    50
NKGGKRDGMA WRDVIEIHET KKTIVRKVDG KDKSIEKTWL YYEMSPYKMM 100
TYQELIWVMH DMGRGLAKIG IKPNGEHKFH IFASTSHKWM KIFLGCISQG 150
IPVVTAYDTL GESGLIHSMV ETESAAIFTD NQLLAKMIVP LQSAKDIKFL 200
IHNEPIDPND RRQNGKLYKA AKDAINKIRE VRPDIKIYSF EEVVKIGKKS 250
KDEVKLHPPE PKDLACIMYT SGSISAPKGV VLTHYNIVSG IAGVGHNVFG 300
WIGSTDRVLS FLPLAHIFEL VFEFEAFYWN GILGYGSVKT LTNTSTRNCK 350
GDLVEFKPTI MIGVAAVWET VRKAILEKIS DLTPVLQKIF WSAYSMKEKS 400
VPCTGFLSRM VFKKVRQATG GHLKYIMNGG SAISIDAQKF FSIVLCPMII 450
GYGLTETVAN ACVLEPDHFE YGIVGDLVGS VTAKLVDVKD LGYYAKNNQG 500
ELLLKGAPVC SEYYKNPIET AVSFTYDGWF RTGDIVEWTP KGQLKIIDRR 550
KNLVKTLNGE YIALEKLESV YRSNSYVKNI CVYADESRVK PVGIVVPNPG 600
PLSKFAVKLR IMKKGEDIEN YIHDKALRNA VFKEMIATAK SQGLVGIELL 650
CGIVFFDEEW TPENGFVTSA QKLKRREILA AVKSEVERVY KENS 694
Length:694
Mass (Da):77,947
Last modified:February 1, 1995 - v1
Checksum:i7AB33044335A5295
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z29647 Genomic DNA. Translation: CAA82755.1.
Z38113 Genomic DNA. Translation: CAA86241.1.
AY558110 Genomic DNA. Translation: AAS56436.1.
BK006942 Genomic DNA. Translation: DAA08537.1.
PIRiB54901.
RefSeqiNP_012257.1. NM_001179359.1.

Genome annotation databases

EnsemblFungiiYIL009W; YIL009W; YIL009W.
GeneIDi854808.
KEGGisce:YIL009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z29647 Genomic DNA. Translation: CAA82755.1 .
Z38113 Genomic DNA. Translation: CAA86241.1 .
AY558110 Genomic DNA. Translation: AAS56436.1 .
BK006942 Genomic DNA. Translation: DAA08537.1 .
PIRi B54901.
RefSeqi NP_012257.1. NM_001179359.1.

3D structure databases

ProteinModelPortali P39002.
SMRi P39002. Positions 100-683.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34983. 32 interactions.
DIPi DIP-4210N.
IntActi P39002. 8 interactions.
MINTi MINT-562678.
STRINGi 4932.YIL009W.

Proteomic databases

MaxQBi P39002.
PaxDbi P39002.
PeptideAtlasi P39002.
PRIDEi P39002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL009W ; YIL009W ; YIL009W .
GeneIDi 854808.
KEGGi sce:YIL009W.

Organism-specific databases

CYGDi YIL009w.
SGDi S000001271. FAA3.

Phylogenomic databases

eggNOGi COG1022.
GeneTreei ENSGT00690000102168.
HOGENOMi HOG000159459.
KOi K01897.
OMAi THENIMA.
OrthoDBi EOG7CCC0K.

Enzyme and pathway databases

BioCyci YEAST:YIL009W-MONOMER.

Miscellaneous databases

NextBioi 977630.

Gene expression databases

Genevestigatori P39002.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic analysis of the role of Saccharomyces cerevisiae acyl-CoA synthetase genes in regulating protein N-myristoylation."
    Johnson D.R., Knoll L.J., Rowley N., Gordon J.I.
    J. Biol. Chem. 269:18037-18046(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1p, Faa2p, and Faa3p."
    Knoll L.J., Johnson D.R., Gordon J.I.
    J. Biol. Chem. 269:16348-16356(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH FRK1.
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLCF3_YEAST
AccessioniPrimary (citable) accession number: P39002
Secondary accession number(s): D6VVS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6440 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi