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Protein

Transcriptional regulatory protein UME6

Gene

UME6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Binds to the URS1 site (5'-AGCCGCCGA-3') and recruits the RPD3 histone deacetylase complex to the promoters to negatively regulate the expression of many genes including CAR1 (arginase), several required for sporulation, mating type switching, inositol metabolism, and oxidative carbon metabolism. Recruits also the ISW2 chromatin remodeling complex to promoters in a second gene repression pathway. Associates with the master regulator of meiosis IME1 in order to activate the expression of meiosis genes. Has both a positive and negative role in regulating phospholipid biosynthesis.21 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi771 – 79828Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. repressing transcription factor binding Source: SGD
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: SGD
  4. transcription factor binding transcription factor activity Source: SGD
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. chromatin remodeling Source: SGD
  2. lipid particle organization Source: SGD
  3. negative regulation of inositol biosynthetic process by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  4. negative regulation of transcription from RNA polymerase II promoter during meiosis Source: SGD
  5. negative regulation of transcription from RNA polymerase II promoter during mitosis Source: SGD
  6. nitrogen catabolite repression of transcription from RNA polymerase II promoter Source: SGD
  7. positive regulation of meiosis by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  8. positive regulation of meiosis by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  9. positive regulation of phosphatidylcholine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  10. positive regulation of phosphatidylserine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  11. positive regulation of transcription from RNA polymerase II promoter during meiosis Source: SGD
  12. pseudohyphal growth Source: SGD
  13. spore germination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29791-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein UME6
Alternative name(s):
Negative transcriptional regulator of IME2
Regulator of inducer of meiosis protein 16
Unscheduled meiotic gene expression protein 6
Gene namesi
Name:UME6
Synonyms:CAR80, CARGR1, NIM2, RIM16
Ordered Locus Names:YDR207C
ORF Names:YD8142.04C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR207c.
SGDiS000002615. UME6.

Subcellular locationi

Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  1. Rpd3L complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi99 – 10911TPVHTPSGSPS → APVHAPAGAPA: Impairs meiotic genes expression, sporulation and interactions with IME1 and RIM11, and abolishes phosphorylation. 1 PublicationAdd
BLAST
Mutagenesisi99 – 1079TPVHTPSGS → APVHAPSGA: Impairs meiotic genes expression and sporulation, reduces the interaction with IME1, and abolishes phosphorylation.
Mutagenesisi99 – 991T → N or A: Impairs meiotic genes expression and sporulation, reduces interactions with IME1 and RIM11, and reduces phosphorylation. 2 Publications
Mutagenesisi103 – 1031T → A: Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation. 1 Publication
Mutagenesisi107 – 1071S → A: Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation. 1 Publication
Mutagenesisi523 – 5231A → S: Impairs SIN3-binding and gene repression activity. 1 Publication
Mutagenesisi524 – 5241A → T: Impairs gene repression activity. 1 Publication
Mutagenesisi525 – 5251Missing : Impairs gene repression activity. 1 Publication
Mutagenesisi526 – 5261V → Q: Impairs gene repression activity. 1 Publication
Mutagenesisi527 – 5271L → P: Impairs SIN3-binding and gene repression activity. 1 Publication
Mutagenesisi528 – 5281S → P: Impairs SIN3-binding and gene repression activity. 1 Publication
Mutagenesisi530 – 5301M → T or V: Impairs SIN3-binding and gene repression activity. 1 Publication
Mutagenesisi635 – 6351K → E: Impairs gene repression activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 836836Transcriptional regulatory protein UME6PRO_0000114987Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei114 – 1141Phosphoserine1 Publication
Modified residuei141 – 1411Phosphoserine2 Publications
Modified residuei150 – 1501Phosphoserine2 Publications
Modified residuei228 – 2281Phosphoserine1 Publication
Modified residuei316 – 3161Phosphoserine1 Publication
Modified residuei318 – 3181Phosphoserine1 Publication
Modified residuei645 – 6451Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by RIM11 and MCK1.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39001.
PaxDbiP39001.
PeptideAtlasiP39001.
PRIDEiP39001.

Expressioni

Gene expression databases

GenevestigatoriP39001.

Interactioni

Subunit structurei

Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6. Interacts with RIM11, MCK1 and IME1.6 Publications

Protein-protein interaction databases

BioGridi32257. 365 interactions.
DIPiDIP-959N.
IntActiP39001. 9 interactions.
MINTiMINT-387730.
STRINGi4932.YDR207C.

Structurei

3D structure databases

ProteinModelPortaliP39001.
SMRiP39001. Positions 769-802.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni508 – 59487SIN3-bindingAdd
BLAST

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG41915.
InParanoidiP39001.
KOiK09243.
OMAiQHELPIS.
OrthoDBiEOG7DZ8TR.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDKARSQSK HMDESNAAAS LLSMETTANN HHYLHNKTSR ATLMNSSQDG
60 70 80 90 100
KKHAEDEVSD GANSRHPTIS SASIESLKTT YDENPLLSIM KSTCAPNNTP
110 120 130 140 150
VHTPSGSPSL KVQSGGDIKD DPKENDTTTT TNTTLQDRRD SDNAVHAAAS
160 170 180 190 200
PLAPSNTPSD PKSLCNGHVA QATDPQISGA IQPQYTATNE DVFPYSSTST
210 220 230 240 250
NSNTATTTIV AGAKKKIHLP PPQAPAVSSP GTTAAGSGAG TGSGIRSRTG
260 270 280 290 300
SDLPLIITSA NKNNGKTTNS PMSILSRNNS TNNNDNNSIQ SSDSRESSNN
310 320 330 340 350
NEIGGYLRGG TKRGGSPSND SQVQHNVHDD QCAVGVAPRN FYFNKDREIT
360 370 380 390 400
DPNVKLDENE SKINISFWLN SKYRDEAYSL NESSSNNASS NTDTPTNSRH
410 420 430 440 450
ANTSSSITSR NNFQHFRFNQ IPSQPPTSAS SFTSTNNNNP QRNNINRGED
460 470 480 490 500
PFATSSRPST GFFYGDLPNR NNRNSPFHTN EQYIPPPPPK YINSKLDGLR
510 520 530 540 550
SRLLLGPNSA SSSTKLDDDL GTAAAVLSNM RSSPYRTHDK PISNVNDMNN
560 570 580 590 600
TNALGVPASR PHSSSFPSKG VLRPILLRIH NSEQQPIFES NNSTAVFDED
610 620 630 640 650
QDQNQDLSPY HLNLNSKKVL DPTFESRTRQ VTWNKNGKRI DRRLSAPEQQ
660 670 680 690 700
QQLEVPPLKK SRRSVGNARV ASQTNSDYNS LGESSTSSAP SSPSLKASSG
710 720 730 740 750
LAYTADYPNA TSPDFAKSKG KNVKPKAKSK AKQSSKKRPN NTTSKSKANN
760 770 780 790 800
SQESNNATSS TSQGTRSRTG CWICRLRKKK CTEERPHCFN CERLKLDCHY
810 820 830
DAFKPDFVSD PKKKQMKLEE IKKKTKEAKR RAMKKK
Length:836
Mass (Da):91,124
Last modified:October 1, 1996 - v2
Checksum:i0DDA0A6B4A157182
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011V → G in BAA04890 (Ref. 3) Curated
Sequence conflicti363 – 3631I → V in AAA34471 (Ref. 1) Curated
Sequence conflicti443 – 4431N → T in AAA34471 (Ref. 1) Curated
Sequence conflicti465 – 4651G → D in AAA34471 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32186 Genomic DNA. Translation: AAA34471.1.
L24539 Unassigned DNA. Translation: AAC14472.1.
D23663 Genomic DNA. Translation: BAA04890.1.
Z68194 Genomic DNA. Translation: CAA92346.1.
BK006938 Genomic DNA. Translation: DAA12048.1.
PIRiS61570.
RefSeqiNP_010493.1. NM_001180515.1.

Genome annotation databases

EnsemblFungiiYDR207C; YDR207C; YDR207C.
GeneIDi851788.
KEGGisce:YDR207C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32186 Genomic DNA. Translation: AAA34471.1.
L24539 Unassigned DNA. Translation: AAC14472.1.
D23663 Genomic DNA. Translation: BAA04890.1.
Z68194 Genomic DNA. Translation: CAA92346.1.
BK006938 Genomic DNA. Translation: DAA12048.1.
PIRiS61570.
RefSeqiNP_010493.1. NM_001180515.1.

3D structure databases

ProteinModelPortaliP39001.
SMRiP39001. Positions 769-802.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32257. 365 interactions.
DIPiDIP-959N.
IntActiP39001. 9 interactions.
MINTiMINT-387730.
STRINGi4932.YDR207C.

Proteomic databases

MaxQBiP39001.
PaxDbiP39001.
PeptideAtlasiP39001.
PRIDEiP39001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR207C; YDR207C; YDR207C.
GeneIDi851788.
KEGGisce:YDR207C.

Organism-specific databases

CYGDiYDR207c.
SGDiS000002615. UME6.

Phylogenomic databases

eggNOGiNOG41915.
InParanoidiP39001.
KOiK09243.
OMAiQHELPIS.
OrthoDBiEOG7DZ8TR.

Enzyme and pathway databases

BioCyciYEAST:G3O-29791-MONOMER.

Miscellaneous databases

NextBioi969606.
PROiP39001.

Gene expression databases

GenevestigatoriP39001.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the UME6/CAR80 gene from Saccharomyces cerevisiae."
    Smart W.C., Park H.-D., Cooper T.G.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sigma 1278B.
  2. "UME6 is a key regulator of nitrogen repression and meiotic development."
    Strich R., Surosky R.T., Steber C.M., Messenguy F., Dubois E., Easton Esposito R.
    Genes Dev. 8:796-810(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  3. Kumeno A.
    Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Identification of negative regulatory genes that govern the expression of early meiotic genes in yeast."
    Strich R., Slater M.R., Easton Esposito R.
    Proc. Natl. Acad. Sci. U.S.A. 86:10018-10022(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Selection for early meiotic mutants in yeast."
    Mitchell A.P., Bowdish K.S.
    Genetics 131:65-72(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The yeast UME6 gene product is required for transcriptional repression mediated by the CAR1 URS1 repressor binding site."
    Park H.-D., Luche R.M., Cooper T.G.
    Nucleic Acids Res. 20:1909-1915(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Positive control of yeast meiotic genes by the negative regulator UME6."
    Bowdish K.S., Yuan H.E., Mitchell A.P.
    Mol. Cell. Biol. 15:2955-2961(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression."
    Steber C.M., Easton Esposito R.
    Proc. Natl. Acad. Sci. U.S.A. 92:12490-12494(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "UME6, a negative regulator of meiosis in Saccharomyces cerevisiae, contains a C-terminal Zn2Cys6 binuclear cluster that binds the URS1 DNA sequence in a zinc-dependent manner."
    Anderson S.F., Steber C.M., Easton Esposito R., Coleman J.E.
    Protein Sci. 4:1832-1843(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, DNA-BINDING.
  12. "Induction of meiosis in Saccharomyces cerevisiae depends on conversion of the transcriptional represssor Ume6 to a positive regulator by its regulated association with the transcriptional activator Ime1."
    Rubin-Bejerano I., Mandel S., Robzyk K., Kassir Y.
    Mol. Cell. Biol. 16:2518-2526(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH IME1.
  13. "The yeast UME6 gene is required for both negative and positive transcriptional regulation of phospholipid biosynthetic gene expression."
    Jackson J.C., Lopes J.M.
    Nucleic Acids Res. 24:1322-1329(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters."
    Kadosh D., Struhl K.
    Cell 89:365-371(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN, INTERACTION WITH SIN3.
  15. "Role of UME6 in transcriptional regulation of a DNA repair gene in Saccharomyces cerevisiae."
    Sweet D.H., Jang Y.K., Sancar G.B.
    Mol. Cell. Biol. 17:6223-6235(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  16. "Interaction of yeast repressor-activator protein Ume6p with glycogen synthase kinase 3 homolog Rim11p."
    Malathi K., Xiao Y., Mitchell A.P.
    Mol. Cell. Biol. 17:7230-7236(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RIM11 AND IME1, PHOSPHORYLATION, MUTAGENESIS OF THR-99 AND 99-THR--SER-109.
  17. "Targeted recruitment of the Sin3-Rpd3 histone deacetylase complex generates a highly localized domain of repressed chromatin in vivo."
    Kadosh D., Struhl K.
    Mol. Cell. Biol. 18:5121-5127(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. "Transcriptional repression by UME6 involves deacetylation of lysine 5 of histone H4 by RPD3."
    Rundlett S.E., Carmen A.A., Suka N., Turner B.M., Grunstein M.
    Nature 392:831-835(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  19. "The Isw2 chromatin remodeling complex represses early meiotic genes upon recruitment by Ume6p."
    Goldmark J.P., Fazzio T.G., Estep P.W., Church G.M., Tsukiyama T.
    Cell 103:423-433(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  20. "Shared roles of yeast glycogen synthase kinase 3 family members in nitrogen-responsive phosphorylation of meiotic regulator Ume6p."
    Xiao Y., Mitchell A.P.
    Mol. Cell. Biol. 20:5447-5453(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF THR-99; THR-103 AND SER-107, INTERACTION WITH MCK1.
  21. "Combinatorial regulation of phospholipid biosynthetic gene expression by the UME6, SIN3 and RPD3 genes."
    Elkhaimi M., Kaadige M.R., Kamath D., Jackson J.C., Biliran H. Jr., Lopes J.M.
    Nucleic Acids Res. 28:3160-3167(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  22. "Identification of the Sin3-binding site in Ume6 defines a two-step process for conversion of Ume6 from a transcriptional repressor to an activator in yeast."
    Washburn B.K., Easton Esposito R.
    Mol. Cell. Biol. 21:2057-2069(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SIN3 AND TEA1, MUTAGENESIS OF ALA-523; ALA-524; ALA-525; VAL-526; LEU-527; SER-528; MET-530 AND LYS-635.
  23. Cited for: FUNCTION.
  24. "Discovery of novel transcription control relationships with gene regulatory networks generated from multiple-disruption full genome expression libraries."
    Aburatani S., Tashiro K., Savoie C.J., Nishizawa M., Hayashi K., Ito Y., Muta S., Yamamoto K., Ogawa M., Enomoto A., Masaki M., Watanabe S., Maki Y., Takahashi Y., Eguchi Y., Sakaki Y., Kuhara S.
    DNA Res. 10:1-8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  25. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  26. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  27. "Yeast Ume6p repressor permits activator binding but restricts TBP binding at the HOP1 promoter."
    Shimizu M., Takahashi K., Lamb T.M., Shindo H., Mitchell A.P.
    Nucleic Acids Res. 31:3033-3037(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  28. "Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex."
    Carrozza M.J., Florens L., Swanson S.K., Shia W.-J., Anderson S., Yates J., Washburn M.P., Workman J.L.
    Biochim. Biophys. Acta 1731:77-87(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RPD3C(L) COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  29. "Identification of functional transcription factor binding sites using closely related Saccharomyces species."
    Doniger S.W., Huh J., Fay J.C.
    Genome Res. 15:701-709(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  30. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  31. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141 AND SER-150, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  32. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114; SER-141; SER-150; SER-228; SER-316; SER-318 AND SER-645, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUME6_YEAST
AccessioniPrimary (citable) accession number: P39001
Secondary accession number(s): D6VSI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: March 4, 2015
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.