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Protein

Transcriptional regulatory protein UME6

Gene

UME6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Binds to the URS1 site (5'-AGCCGCCGA-3') and recruits the RPD3 histone deacetylase complex to the promoters to negatively regulate the expression of many genes including CAR1 (arginase), several required for sporulation, mating type switching, inositol metabolism, and oxidative carbon metabolism. Recruits also the ISW2 chromatin remodeling complex to promoters in a second gene repression pathway. Associates with the master regulator of meiosis IME1 in order to activate the expression of meiosis genes. Has both a positive and negative role in regulating phospholipid biosynthesis.21 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi771 – 798Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

  • repressing transcription factor binding Source: SGD
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: SGD
  • transcription factor activity, transcription factor binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • chromatin remodeling Source: SGD
  • lipid particle organization Source: SGD
  • negative regulation of inositol biosynthetic process by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter during mitosis Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle Source: SGD
  • nitrogen catabolite repression of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of meiosis by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of meiosis by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of phosphatidylcholine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of phosphatidylserine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle Source: SGD
  • pseudohyphal growth Source: SGD
  • spore germination Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29791-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein UME6
Alternative name(s):
Negative transcriptional regulator of IME2
Regulator of inducer of meiosis protein 16
Unscheduled meiotic gene expression protein 6
Gene namesi
Name:UME6
Synonyms:CAR80, CARGR1, NIM2, RIM16
Ordered Locus Names:YDR207C
ORF Names:YD8142.04C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR207C.
SGDiS000002615. UME6.

Subcellular locationi

GO - Cellular componenti

  • Rpd3L complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi99 – 109TPVHTPSGSPS → APVHAPAGAPA: Impairs meiotic genes expression, sporulation and interactions with IME1 and RIM11, and abolishes phosphorylation. 1 PublicationAdd BLAST11
Mutagenesisi99 – 107TPVHTPSGS → APVHAPSGA: Impairs meiotic genes expression and sporulation, reduces the interaction with IME1, and abolishes phosphorylation. 9
Mutagenesisi99T → N or A: Impairs meiotic genes expression and sporulation, reduces interactions with IME1 and RIM11, and reduces phosphorylation. 2 Publications1
Mutagenesisi103T → A: Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation. 1 Publication1
Mutagenesisi107S → A: Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation. 1 Publication1
Mutagenesisi523A → S: Impairs SIN3-binding and gene repression activity. 1 Publication1
Mutagenesisi524A → T: Impairs gene repression activity. 1 Publication1
Mutagenesisi525Missing : Impairs gene repression activity. 1 Publication1
Mutagenesisi526V → Q: Impairs gene repression activity. 1 Publication1
Mutagenesisi527L → P: Impairs SIN3-binding and gene repression activity. 1 Publication1
Mutagenesisi528S → P: Impairs SIN3-binding and gene repression activity. 1 Publication1
Mutagenesisi530M → T or V: Impairs SIN3-binding and gene repression activity. 1 Publication1
Mutagenesisi635K → E: Impairs gene repression activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149871 – 836Transcriptional regulatory protein UME6Add BLAST836

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei114PhosphoserineCombined sources1
Modified residuei141PhosphoserineCombined sources1
Modified residuei150PhosphoserineCombined sources1
Modified residuei228PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1
Modified residuei318PhosphoserineCombined sources1
Modified residuei645PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by RIM11 and MCK1.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39001.
PRIDEiP39001.

PTM databases

iPTMnetiP39001.

Interactioni

Subunit structurei

Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6. Interacts with RIM11, MCK1 and IME1.6 Publications

GO - Molecular functioni

  • repressing transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi32257. 366 interactors.
DIPiDIP-959N.
IntActiP39001. 9 interactors.
MINTiMINT-387730.

Structurei

3D structure databases

ProteinModelPortaliP39001.
SMRiP39001.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni508 – 594SIN3-bindingAdd BLAST87

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP39001.
KOiK09243.
OMAiCTEERPR.
OrthoDBiEOG092C1LOZ.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDKARSQSK HMDESNAAAS LLSMETTANN HHYLHNKTSR ATLMNSSQDG
60 70 80 90 100
KKHAEDEVSD GANSRHPTIS SASIESLKTT YDENPLLSIM KSTCAPNNTP
110 120 130 140 150
VHTPSGSPSL KVQSGGDIKD DPKENDTTTT TNTTLQDRRD SDNAVHAAAS
160 170 180 190 200
PLAPSNTPSD PKSLCNGHVA QATDPQISGA IQPQYTATNE DVFPYSSTST
210 220 230 240 250
NSNTATTTIV AGAKKKIHLP PPQAPAVSSP GTTAAGSGAG TGSGIRSRTG
260 270 280 290 300
SDLPLIITSA NKNNGKTTNS PMSILSRNNS TNNNDNNSIQ SSDSRESSNN
310 320 330 340 350
NEIGGYLRGG TKRGGSPSND SQVQHNVHDD QCAVGVAPRN FYFNKDREIT
360 370 380 390 400
DPNVKLDENE SKINISFWLN SKYRDEAYSL NESSSNNASS NTDTPTNSRH
410 420 430 440 450
ANTSSSITSR NNFQHFRFNQ IPSQPPTSAS SFTSTNNNNP QRNNINRGED
460 470 480 490 500
PFATSSRPST GFFYGDLPNR NNRNSPFHTN EQYIPPPPPK YINSKLDGLR
510 520 530 540 550
SRLLLGPNSA SSSTKLDDDL GTAAAVLSNM RSSPYRTHDK PISNVNDMNN
560 570 580 590 600
TNALGVPASR PHSSSFPSKG VLRPILLRIH NSEQQPIFES NNSTAVFDED
610 620 630 640 650
QDQNQDLSPY HLNLNSKKVL DPTFESRTRQ VTWNKNGKRI DRRLSAPEQQ
660 670 680 690 700
QQLEVPPLKK SRRSVGNARV ASQTNSDYNS LGESSTSSAP SSPSLKASSG
710 720 730 740 750
LAYTADYPNA TSPDFAKSKG KNVKPKAKSK AKQSSKKRPN NTTSKSKANN
760 770 780 790 800
SQESNNATSS TSQGTRSRTG CWICRLRKKK CTEERPHCFN CERLKLDCHY
810 820 830
DAFKPDFVSD PKKKQMKLEE IKKKTKEAKR RAMKKK
Length:836
Mass (Da):91,124
Last modified:October 1, 1996 - v2
Checksum:i0DDA0A6B4A157182
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101V → G in BAA04890 (Ref. 3) Curated1
Sequence conflicti363I → V in AAA34471 (Ref. 1) Curated1
Sequence conflicti443N → T in AAA34471 (Ref. 1) Curated1
Sequence conflicti465G → D in AAA34471 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32186 Genomic DNA. Translation: AAA34471.1.
L24539 Unassigned DNA. Translation: AAC14472.1.
D23663 Genomic DNA. Translation: BAA04890.1.
Z68194 Genomic DNA. Translation: CAA92346.1.
BK006938 Genomic DNA. Translation: DAA12048.1.
PIRiS61570.
RefSeqiNP_010493.1. NM_001180515.1.

Genome annotation databases

EnsemblFungiiYDR207C; YDR207C; YDR207C.
GeneIDi851788.
KEGGisce:YDR207C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32186 Genomic DNA. Translation: AAA34471.1.
L24539 Unassigned DNA. Translation: AAC14472.1.
D23663 Genomic DNA. Translation: BAA04890.1.
Z68194 Genomic DNA. Translation: CAA92346.1.
BK006938 Genomic DNA. Translation: DAA12048.1.
PIRiS61570.
RefSeqiNP_010493.1. NM_001180515.1.

3D structure databases

ProteinModelPortaliP39001.
SMRiP39001.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32257. 366 interactors.
DIPiDIP-959N.
IntActiP39001. 9 interactors.
MINTiMINT-387730.

PTM databases

iPTMnetiP39001.

Proteomic databases

MaxQBiP39001.
PRIDEiP39001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR207C; YDR207C; YDR207C.
GeneIDi851788.
KEGGisce:YDR207C.

Organism-specific databases

EuPathDBiFungiDB:YDR207C.
SGDiS000002615. UME6.

Phylogenomic databases

InParanoidiP39001.
KOiK09243.
OMAiCTEERPR.
OrthoDBiEOG092C1LOZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-29791-MONOMER.

Miscellaneous databases

PROiP39001.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUME6_YEAST
AccessioniPrimary (citable) accession number: P39001
Secondary accession number(s): D6VSI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.