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Protein

Transcriptional regulatory protein UME6

Gene

UME6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Binds to the URS1 site (5'-AGCCGCCGA-3') and recruits the RPD3 histone deacetylase complex to the promoters to negatively regulate the expression of many genes including CAR1 (arginase), several required for sporulation, mating type switching, inositol metabolism, and oxidative carbon metabolism. Recruits also the ISW2 chromatin remodeling complex to promoters in a second gene repression pathway. Associates with the master regulator of meiosis IME1 in order to activate the expression of meiosis genes. Has both a positive and negative role in regulating phospholipid biosynthesis.21 Publications

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi771 – 798Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

  • repressing transcription factor binding Source: SGD
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: SGD
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: SGD
  • transcription factor activity, transcription factor binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromatin remodeling Source: SGD
  • lipid particle organization Source: SGD
  • negative regulation of inositol biosynthetic process by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of transcription by RNA polymerase II Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle Source: SGD
  • nitrogen catabolite repression of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of phosphatidylcholine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of phosphatidylserine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle Source: SGD
  • pseudohyphal growth Source: SGD
  • spore germination Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Chromatin regulator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29791-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein UME6
Alternative name(s):
Negative transcriptional regulator of IME2
Regulator of inducer of meiosis protein 16
Unscheduled meiotic gene expression protein 6
Gene namesi
Name:UME6
Synonyms:CAR80, CARGR1, NIM2, RIM16
Ordered Locus Names:YDR207C
ORF Names:YD8142.04C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR207C
SGDiS000002615 UME6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi99 – 109TPVHTPSGSPS → APVHAPAGAPA: Impairs meiotic genes expression, sporulation and interactions with IME1 and RIM11, and abolishes phosphorylation. 1 PublicationAdd BLAST11
Mutagenesisi99 – 107TPVHTPSGS → APVHAPSGA: Impairs meiotic genes expression and sporulation, reduces the interaction with IME1, and abolishes phosphorylation. 9
Mutagenesisi99T → N or A: Impairs meiotic genes expression and sporulation, reduces interactions with IME1 and RIM11, and reduces phosphorylation. 2 Publications1
Mutagenesisi103T → A: Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation. 1 Publication1
Mutagenesisi107S → A: Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation. 1 Publication1
Mutagenesisi523A → S: Impairs SIN3-binding and gene repression activity. 1 Publication1
Mutagenesisi524A → T: Impairs gene repression activity. 1 Publication1
Mutagenesisi525Missing : Impairs gene repression activity. 1 Publication1
Mutagenesisi526V → Q: Impairs gene repression activity. 1 Publication1
Mutagenesisi527L → P: Impairs SIN3-binding and gene repression activity. 1 Publication1
Mutagenesisi528S → P: Impairs SIN3-binding and gene repression activity. 1 Publication1
Mutagenesisi530M → T or V: Impairs SIN3-binding and gene repression activity. 1 Publication1
Mutagenesisi635K → E: Impairs gene repression activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149871 – 836Transcriptional regulatory protein UME6Add BLAST836

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei114PhosphoserineCombined sources1
Modified residuei141PhosphoserineCombined sources1
Modified residuei150PhosphoserineCombined sources1
Modified residuei228PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1
Modified residuei318PhosphoserineCombined sources1
Modified residuei645PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by RIM11 and MCK1.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39001
PaxDbiP39001
PRIDEiP39001

PTM databases

iPTMnetiP39001

Interactioni

Subunit structurei

Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6. Interacts with RIM11, MCK1 and IME1.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HTA2P049122EBI-20086,EBI-8076

GO - Molecular functioni

  • repressing transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi32257, 750 interactors
DIPiDIP-959N
ELMiP39001
IntActiP39001, 21 interactors
MINTiP39001
STRINGi4932.YDR207C

Structurei

3D structure databases

ProteinModelPortaliP39001
SMRiP39001
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni508 – 594SIN3-bindingAdd BLAST87

Phylogenomic databases

InParanoidiP39001
KOiK09243
OMAiCTEERPR
OrthoDBiEOG092C1LOZ

Family and domain databases

CDDicd00067 GAL4, 1 hit
Gene3Di4.10.240.10, 1 hit
InterProiView protein in InterPro
IPR001138 Zn2-C6_fun-type_DNA-bd
IPR036864 Zn2-C6_fun-type_DNA-bd_sf
PfamiView protein in Pfam
PF00172 Zn_clus, 1 hit
SMARTiView protein in SMART
SM00066 GAL4, 1 hit
SUPFAMiSSF57701 SSF57701, 1 hit
PROSITEiView protein in PROSITE
PS00463 ZN2_CY6_FUNGAL_1, 1 hit
PS50048 ZN2_CY6_FUNGAL_2, 1 hit

Sequencei

Sequence statusi: Complete.

P39001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDKARSQSK HMDESNAAAS LLSMETTANN HHYLHNKTSR ATLMNSSQDG
60 70 80 90 100
KKHAEDEVSD GANSRHPTIS SASIESLKTT YDENPLLSIM KSTCAPNNTP
110 120 130 140 150
VHTPSGSPSL KVQSGGDIKD DPKENDTTTT TNTTLQDRRD SDNAVHAAAS
160 170 180 190 200
PLAPSNTPSD PKSLCNGHVA QATDPQISGA IQPQYTATNE DVFPYSSTST
210 220 230 240 250
NSNTATTTIV AGAKKKIHLP PPQAPAVSSP GTTAAGSGAG TGSGIRSRTG
260 270 280 290 300
SDLPLIITSA NKNNGKTTNS PMSILSRNNS TNNNDNNSIQ SSDSRESSNN
310 320 330 340 350
NEIGGYLRGG TKRGGSPSND SQVQHNVHDD QCAVGVAPRN FYFNKDREIT
360 370 380 390 400
DPNVKLDENE SKINISFWLN SKYRDEAYSL NESSSNNASS NTDTPTNSRH
410 420 430 440 450
ANTSSSITSR NNFQHFRFNQ IPSQPPTSAS SFTSTNNNNP QRNNINRGED
460 470 480 490 500
PFATSSRPST GFFYGDLPNR NNRNSPFHTN EQYIPPPPPK YINSKLDGLR
510 520 530 540 550
SRLLLGPNSA SSSTKLDDDL GTAAAVLSNM RSSPYRTHDK PISNVNDMNN
560 570 580 590 600
TNALGVPASR PHSSSFPSKG VLRPILLRIH NSEQQPIFES NNSTAVFDED
610 620 630 640 650
QDQNQDLSPY HLNLNSKKVL DPTFESRTRQ VTWNKNGKRI DRRLSAPEQQ
660 670 680 690 700
QQLEVPPLKK SRRSVGNARV ASQTNSDYNS LGESSTSSAP SSPSLKASSG
710 720 730 740 750
LAYTADYPNA TSPDFAKSKG KNVKPKAKSK AKQSSKKRPN NTTSKSKANN
760 770 780 790 800
SQESNNATSS TSQGTRSRTG CWICRLRKKK CTEERPHCFN CERLKLDCHY
810 820 830
DAFKPDFVSD PKKKQMKLEE IKKKTKEAKR RAMKKK
Length:836
Mass (Da):91,124
Last modified:October 1, 1996 - v2
Checksum:i0DDA0A6B4A157182
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101V → G in BAA04890 (Ref. 3) Curated1
Sequence conflicti363I → V in AAA34471 (Ref. 1) Curated1
Sequence conflicti443N → T in AAA34471 (Ref. 1) Curated1
Sequence conflicti465G → D in AAA34471 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32186 Genomic DNA Translation: AAA34471.1
L24539 Unassigned DNA Translation: AAC14472.1
D23663 Genomic DNA Translation: BAA04890.1
Z68194 Genomic DNA Translation: CAA92346.1
BK006938 Genomic DNA Translation: DAA12048.1
PIRiS61570
RefSeqiNP_010493.1, NM_001180515.1

Genome annotation databases

EnsemblFungiiYDR207C; YDR207C; YDR207C
GeneIDi851788
KEGGisce:YDR207C

Similar proteinsi

Entry informationi

Entry nameiUME6_YEAST
AccessioniPrimary (citable) accession number: P39001
Secondary accession number(s): D6VSI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health