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P39001

- UME6_YEAST

UniProt

P39001 - UME6_YEAST

Protein

Transcriptional regulatory protein UME6

Gene

UME6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Binds to the URS1 site (5'-AGCCGCCGA-3') and recruits the RPD3 histone deacetylase complex to the promoters to negatively regulate the expression of many genes including CAR1 (arginase), several required for sporulation, mating type switching, inositol metabolism, and oxidative carbon metabolism. Recruits also the ISW2 chromatin remodeling complex to promoters in a second gene repression pathway. Associates with the master regulator of meiosis IME1 in order to activate the expression of meiosis genes. Has both a positive and negative role in regulating phospholipid biosynthesis.21 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi771 – 79828Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. repressing transcription factor binding Source: SGD
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: SGD
    4. transcription factor binding transcription factor activity Source: SGD
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. chromatin remodeling Source: SGD
    2. lipid particle organization Source: SGD
    3. negative regulation of inositol biosynthetic process by negative regulation of transcription from RNA polymerase II promoter Source: SGD
    4. negative regulation of transcription from RNA polymerase II promoter during meiosis Source: SGD
    5. negative regulation of transcription from RNA polymerase II promoter during mitosis Source: SGD
    6. nitrogen catabolite repression of transcription from RNA polymerase II promoter Source: SGD
    7. positive regulation of meiosis by negative regulation of transcription from RNA polymerase II promoter Source: SGD
    8. positive regulation of meiosis by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    9. positive regulation of phosphatidylcholine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    10. positive regulation of phosphatidylserine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    11. positive regulation of transcription from RNA polymerase II promoter during meiosis Source: SGD
    12. pseudohyphal growth Source: SGD
    13. spore germination Source: SGD

    Keywords - Molecular functioni

    Activator, Chromatin regulator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29791-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional regulatory protein UME6
    Alternative name(s):
    Negative transcriptional regulator of IME2
    Regulator of inducer of meiosis protein 16
    Unscheduled meiotic gene expression protein 6
    Gene namesi
    Name:UME6
    Synonyms:CAR80, CARGR1, NIM2, RIM16
    Ordered Locus Names:YDR207C
    ORF Names:YD8142.04C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR207c.
    SGDiS000002615. UME6.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. Rpd3L complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi99 – 10911TPVHTPSGSPS → APVHAPAGAPA: Impairs meiotic genes expression, sporulation and interactions with IME1 and RIM11, and abolishes phosphorylation. 2 PublicationsAdd
    BLAST
    Mutagenesisi99 – 1079TPVHTPSGS → APVHAPSGA: Impairs meiotic genes expression and sporulation, reduces the interaction with IME1, and abolishes phosphorylation. 2 Publications
    Mutagenesisi99 – 991T → N or A: Impairs meiotic genes expression and sporulation, reduces interactions with IME1 and RIM11, and reduces phosphorylation. 2 Publications
    Mutagenesisi103 – 1031T → A: Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation. 1 Publication
    Mutagenesisi107 – 1071S → A: Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation. 1 Publication
    Mutagenesisi523 – 5231A → S: Impairs SIN3-binding and gene repression activity. 1 Publication
    Mutagenesisi524 – 5241A → T: Impairs gene repression activity. 1 Publication
    Mutagenesisi525 – 5251Missing: Impairs gene repression activity. 1 Publication
    Mutagenesisi526 – 5261V → Q: Impairs gene repression activity. 1 Publication
    Mutagenesisi527 – 5271L → P: Impairs SIN3-binding and gene repression activity. 1 Publication
    Mutagenesisi528 – 5281S → P: Impairs SIN3-binding and gene repression activity. 1 Publication
    Mutagenesisi530 – 5301M → T or V: Impairs SIN3-binding and gene repression activity. 1 Publication
    Mutagenesisi635 – 6351K → E: Impairs gene repression activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 836836Transcriptional regulatory protein UME6PRO_0000114987Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei114 – 1141Phosphoserine1 Publication
    Modified residuei141 – 1411Phosphoserine2 Publications
    Modified residuei150 – 1501Phosphoserine2 Publications
    Modified residuei228 – 2281Phosphoserine1 Publication
    Modified residuei316 – 3161Phosphoserine1 Publication
    Modified residuei318 – 3181Phosphoserine1 Publication
    Modified residuei645 – 6451Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated by RIM11 and MCK1.4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39001.
    PaxDbiP39001.
    PeptideAtlasiP39001.
    PRIDEiP39001.

    Expressioni

    Gene expression databases

    GenevestigatoriP39001.

    Interactioni

    Subunit structurei

    Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6. Interacts with RIM11, MCK1 and IME1.6 Publications

    Protein-protein interaction databases

    BioGridi32257. 363 interactions.
    DIPiDIP-959N.
    IntActiP39001. 9 interactions.
    MINTiMINT-387730.
    STRINGi4932.YDR207C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39001.
    SMRiP39001. Positions 769-802.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni508 – 59487SIN3-bindingAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG41915.
    KOiK09243.
    OMAiQHELPIS.
    OrthoDBiEOG7DZ8TR.

    Family and domain databases

    Gene3Di4.10.240.10. 1 hit.
    InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view]
    PfamiPF00172. Zn_clus. 1 hit.
    [Graphical view]
    SMARTiSM00066. GAL4. 1 hit.
    [Graphical view]
    SUPFAMiSSF57701. SSF57701. 1 hit.
    PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39001-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLDKARSQSK HMDESNAAAS LLSMETTANN HHYLHNKTSR ATLMNSSQDG    50
    KKHAEDEVSD GANSRHPTIS SASIESLKTT YDENPLLSIM KSTCAPNNTP 100
    VHTPSGSPSL KVQSGGDIKD DPKENDTTTT TNTTLQDRRD SDNAVHAAAS 150
    PLAPSNTPSD PKSLCNGHVA QATDPQISGA IQPQYTATNE DVFPYSSTST 200
    NSNTATTTIV AGAKKKIHLP PPQAPAVSSP GTTAAGSGAG TGSGIRSRTG 250
    SDLPLIITSA NKNNGKTTNS PMSILSRNNS TNNNDNNSIQ SSDSRESSNN 300
    NEIGGYLRGG TKRGGSPSND SQVQHNVHDD QCAVGVAPRN FYFNKDREIT 350
    DPNVKLDENE SKINISFWLN SKYRDEAYSL NESSSNNASS NTDTPTNSRH 400
    ANTSSSITSR NNFQHFRFNQ IPSQPPTSAS SFTSTNNNNP QRNNINRGED 450
    PFATSSRPST GFFYGDLPNR NNRNSPFHTN EQYIPPPPPK YINSKLDGLR 500
    SRLLLGPNSA SSSTKLDDDL GTAAAVLSNM RSSPYRTHDK PISNVNDMNN 550
    TNALGVPASR PHSSSFPSKG VLRPILLRIH NSEQQPIFES NNSTAVFDED 600
    QDQNQDLSPY HLNLNSKKVL DPTFESRTRQ VTWNKNGKRI DRRLSAPEQQ 650
    QQLEVPPLKK SRRSVGNARV ASQTNSDYNS LGESSTSSAP SSPSLKASSG 700
    LAYTADYPNA TSPDFAKSKG KNVKPKAKSK AKQSSKKRPN NTTSKSKANN 750
    SQESNNATSS TSQGTRSRTG CWICRLRKKK CTEERPHCFN CERLKLDCHY 800
    DAFKPDFVSD PKKKQMKLEE IKKKTKEAKR RAMKKK 836
    Length:836
    Mass (Da):91,124
    Last modified:October 1, 1996 - v2
    Checksum:i0DDA0A6B4A157182
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti101 – 1011V → G in BAA04890. 1 PublicationCurated
    Sequence conflicti363 – 3631I → V in AAA34471. 1 PublicationCurated
    Sequence conflicti443 – 4431N → T in AAA34471. 1 PublicationCurated
    Sequence conflicti465 – 4651G → D in AAA34471. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32186 Genomic DNA. Translation: AAA34471.1.
    L24539 Unassigned DNA. Translation: AAC14472.1.
    D23663 Genomic DNA. Translation: BAA04890.1.
    Z68194 Genomic DNA. Translation: CAA92346.1.
    BK006938 Genomic DNA. Translation: DAA12048.1.
    PIRiS61570.
    RefSeqiNP_010493.1. NM_001180515.1.

    Genome annotation databases

    EnsemblFungiiYDR207C; YDR207C; YDR207C.
    GeneIDi851788.
    KEGGisce:YDR207C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32186 Genomic DNA. Translation: AAA34471.1 .
    L24539 Unassigned DNA. Translation: AAC14472.1 .
    D23663 Genomic DNA. Translation: BAA04890.1 .
    Z68194 Genomic DNA. Translation: CAA92346.1 .
    BK006938 Genomic DNA. Translation: DAA12048.1 .
    PIRi S61570.
    RefSeqi NP_010493.1. NM_001180515.1.

    3D structure databases

    ProteinModelPortali P39001.
    SMRi P39001. Positions 769-802.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32257. 363 interactions.
    DIPi DIP-959N.
    IntActi P39001. 9 interactions.
    MINTi MINT-387730.
    STRINGi 4932.YDR207C.

    Proteomic databases

    MaxQBi P39001.
    PaxDbi P39001.
    PeptideAtlasi P39001.
    PRIDEi P39001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR207C ; YDR207C ; YDR207C .
    GeneIDi 851788.
    KEGGi sce:YDR207C.

    Organism-specific databases

    CYGDi YDR207c.
    SGDi S000002615. UME6.

    Phylogenomic databases

    eggNOGi NOG41915.
    KOi K09243.
    OMAi QHELPIS.
    OrthoDBi EOG7DZ8TR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29791-MONOMER.

    Miscellaneous databases

    NextBioi 969606.
    PROi P39001.

    Gene expression databases

    Genevestigatori P39001.

    Family and domain databases

    Gene3Di 4.10.240.10. 1 hit.
    InterProi IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view ]
    Pfami PF00172. Zn_clus. 1 hit.
    [Graphical view ]
    SMARTi SM00066. GAL4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57701. SSF57701. 1 hit.
    PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the UME6/CAR80 gene from Saccharomyces cerevisiae."
      Smart W.C., Park H.-D., Cooper T.G.
      Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sigma 1278B.
    2. "UME6 is a key regulator of nitrogen repression and meiotic development."
      Strich R., Surosky R.T., Steber C.M., Messenguy F., Dubois E., Easton Esposito R.
      Genes Dev. 8:796-810(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    3. Kumeno A.
      Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Identification of negative regulatory genes that govern the expression of early meiotic genes in yeast."
      Strich R., Slater M.R., Easton Esposito R.
      Proc. Natl. Acad. Sci. U.S.A. 86:10018-10022(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Selection for early meiotic mutants in yeast."
      Mitchell A.P., Bowdish K.S.
      Genetics 131:65-72(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The yeast UME6 gene product is required for transcriptional repression mediated by the CAR1 URS1 repressor binding site."
      Park H.-D., Luche R.M., Cooper T.G.
      Nucleic Acids Res. 20:1909-1915(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Positive control of yeast meiotic genes by the negative regulator UME6."
      Bowdish K.S., Yuan H.E., Mitchell A.P.
      Mol. Cell. Biol. 15:2955-2961(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression."
      Steber C.M., Easton Esposito R.
      Proc. Natl. Acad. Sci. U.S.A. 92:12490-12494(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "UME6, a negative regulator of meiosis in Saccharomyces cerevisiae, contains a C-terminal Zn2Cys6 binuclear cluster that binds the URS1 DNA sequence in a zinc-dependent manner."
      Anderson S.F., Steber C.M., Easton Esposito R., Coleman J.E.
      Protein Sci. 4:1832-1843(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN, DNA-BINDING.
    12. "Induction of meiosis in Saccharomyces cerevisiae depends on conversion of the transcriptional represssor Ume6 to a positive regulator by its regulated association with the transcriptional activator Ime1."
      Rubin-Bejerano I., Mandel S., Robzyk K., Kassir Y.
      Mol. Cell. Biol. 16:2518-2526(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH IME1.
    13. "The yeast UME6 gene is required for both negative and positive transcriptional regulation of phospholipid biosynthetic gene expression."
      Jackson J.C., Lopes J.M.
      Nucleic Acids Res. 24:1322-1329(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters."
      Kadosh D., Struhl K.
      Cell 89:365-371(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DOMAIN, INTERACTION WITH SIN3.
    15. "Role of UME6 in transcriptional regulation of a DNA repair gene in Saccharomyces cerevisiae."
      Sweet D.H., Jang Y.K., Sancar G.B.
      Mol. Cell. Biol. 17:6223-6235(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING.
    16. "Interaction of yeast repressor-activator protein Ume6p with glycogen synthase kinase 3 homolog Rim11p."
      Malathi K., Xiao Y., Mitchell A.P.
      Mol. Cell. Biol. 17:7230-7236(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RIM11 AND IME1, PHOSPHORYLATION, MUTAGENESIS OF THR-99 AND 99-THR--SER-109.
    17. "Targeted recruitment of the Sin3-Rpd3 histone deacetylase complex generates a highly localized domain of repressed chromatin in vivo."
      Kadosh D., Struhl K.
      Mol. Cell. Biol. 18:5121-5127(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    18. "Transcriptional repression by UME6 involves deacetylation of lysine 5 of histone H4 by RPD3."
      Rundlett S.E., Carmen A.A., Suka N., Turner B.M., Grunstein M.
      Nature 392:831-835(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    19. "The Isw2 chromatin remodeling complex represses early meiotic genes upon recruitment by Ume6p."
      Goldmark J.P., Fazzio T.G., Estep P.W., Church G.M., Tsukiyama T.
      Cell 103:423-433(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING.
    20. "Shared roles of yeast glycogen synthase kinase 3 family members in nitrogen-responsive phosphorylation of meiotic regulator Ume6p."
      Xiao Y., Mitchell A.P.
      Mol. Cell. Biol. 20:5447-5453(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF THR-99; THR-103 AND SER-107, INTERACTION WITH MCK1.
    21. "Combinatorial regulation of phospholipid biosynthetic gene expression by the UME6, SIN3 and RPD3 genes."
      Elkhaimi M., Kaadige M.R., Kamath D., Jackson J.C., Biliran H. Jr., Lopes J.M.
      Nucleic Acids Res. 28:3160-3167(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    22. "Identification of the Sin3-binding site in Ume6 defines a two-step process for conversion of Ume6 from a transcriptional repressor to an activator in yeast."
      Washburn B.K., Easton Esposito R.
      Mol. Cell. Biol. 21:2057-2069(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SIN3 AND TEA1, MUTAGENESIS OF ALA-523; ALA-524; ALA-525; VAL-526; LEU-527; SER-528; MET-530 AND LYS-635.
    23. Cited for: FUNCTION.
    24. "Discovery of novel transcription control relationships with gene regulatory networks generated from multiple-disruption full genome expression libraries."
      Aburatani S., Tashiro K., Savoie C.J., Nishizawa M., Hayashi K., Ito Y., Muta S., Yamamoto K., Ogawa M., Enomoto A., Masaki M., Watanabe S., Maki Y., Takahashi Y., Eguchi Y., Sakaki Y., Kuhara S.
      DNA Res. 10:1-8(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    25. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    26. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    27. "Yeast Ume6p repressor permits activator binding but restricts TBP binding at the HOP1 promoter."
      Shimizu M., Takahashi K., Lamb T.M., Shindo H., Mitchell A.P.
      Nucleic Acids Res. 31:3033-3037(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    28. "Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex."
      Carrozza M.J., Florens L., Swanson S.K., Shia W.-J., Anderson S., Yates J., Washburn M.P., Workman J.L.
      Biochim. Biophys. Acta 1731:77-87(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RPD3C(L) COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    29. "Identification of functional transcription factor binding sites using closely related Saccharomyces species."
      Doniger S.W., Huh J., Fay J.C.
      Genome Res. 15:701-709(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING.
    30. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    31. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141 AND SER-150, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    32. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114; SER-141; SER-150; SER-228; SER-316; SER-318 AND SER-645, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUME6_YEAST
    AccessioniPrimary (citable) accession number: P39001
    Secondary accession number(s): D6VSI8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 217 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3