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P38999

- LYS9_YEAST

UniProt

P38999 - LYS9_YEAST

Protein

Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]

Gene

LYS9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH.

    Pathwayi

    GO - Molecular functioni

    1. saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity Source: SGD

    GO - Biological processi

    1. lysine biosynthetic process via aminoadipic acid Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciYEAST:MONOMER3O-363.
    UniPathwayiUPA00033; UER00033.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Saccharopine dehydrogenase [NADP(+), L-glutamate-forming] (EC:1.5.1.10)
    Alternative name(s):
    Saccharopine reductase
    Gene namesi
    Name:LYS9
    Synonyms:LYS13
    Ordered Locus Names:YNR050C
    ORF Names:N3461
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNR050c.
    SGDiS000005333. LYS9.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 446446Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]PRO_0000212839Add
    BLAST

    Proteomic databases

    MaxQBiP38999.
    PaxDbiP38999.
    PeptideAtlasiP38999.

    Expressioni

    Gene expression databases

    GenevestigatoriP38999.

    Interactioni

    Subunit structurei

    Interacts with TRM112.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-10294,EBI-10294

    Protein-protein interaction databases

    BioGridi35875. 14 interactions.
    DIPiDIP-776N.
    IntActiP38999. 9 interactions.
    MINTiMINT-425978.
    STRINGi4932.YNR050C.

    Structurei

    Secondary structure

    1
    446
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 86
    Helixi14 – 229
    Beta strandi27 – 359
    Helixi36 – 438
    Turni44 – 474
    Beta strandi49 – 524
    Helixi58 – 669
    Beta strandi68 – 736
    Helixi77 – 793
    Helixi80 – 9011
    Beta strandi93 – 964
    Helixi102 – 11413
    Beta strandi117 – 1193
    Turni124 – 1263
    Helixi128 – 14215
    Beta strandi146 – 15813
    Helixi160 – 1623
    Beta strandi171 – 1733
    Helixi176 – 1805
    Helixi181 – 1833
    Beta strandi186 – 1905
    Beta strandi193 – 1975
    Turni199 – 2013
    Helixi202 – 2054
    Beta strandi217 – 2215
    Helixi228 – 2314
    Beta strandi238 – 24710
    Helixi250 – 25910
    Turni260 – 2634
    Helixi269 – 2713
    Helixi277 – 2859
    Beta strandi288 – 2914
    Helixi292 – 3009
    Helixi308 – 32013
    Turni321 – 3244
    Helixi335 – 34612
    Beta strandi355 – 36612
    Beta strandi372 – 38211
    Helixi390 – 40718
    Beta strandi414 – 4174
    Helixi422 – 43615
    Beta strandi441 – 4444

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AXQX-ray1.70A1-446[»]
    ProteinModelPortaliP38999.
    SMRiP38999. Positions 2-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38999.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the saccharopine dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG1748.
    GeneTreeiENSGT00390000013249.
    HOGENOMiHOG000023158.
    KOiK00293.
    OMAiYAVKTIE.
    OrthoDBiEOG7FJH8S.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR005097. Saccharopine_DH/HSpermid_syn.
    [Graphical view]
    PfamiPF03435. Saccharop_dh. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38999-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKNVLLLGS GFVAQPVIDT LAANDDINVT VACRTLANAQ ALAKPSGSKA    50
    ISLDVTDDSA LDKVLADNDV VISLIPYTFH PNVVKSAIRT KTDVVTSSYI 100
    SPALRELEPE IVKAGITVMN EIGLDPGIDH LYAVKTIDEV HRAGGKLKSF 150
    LSYCGGLPAP EDSDNPLGYK FSWSSRGVLL ALRNSAKYWK DGKIETVSSE 200
    DLMATAKPYF IYPGYAFVCY PNRDSTLFKD LYHIPEAETV IRGTLRYQGF 250
    PEFVKALVDM GMLKDDANEI FSKPIAWNEA LKQYLGAKST SKEDLIASID 300
    SKATWKDDED RERILSGFAW LGLFSDAKIT PRGNALDTLC ARLEELMQYE 350
    DNERDMVVLQ HKFGIEWADG TTETRTSTLV DYGKVGGYSS MAATVGYPVA 400
    IATKFVLDGT IKGPGLLAPY SPEINDPIMK ELKDKYGIYL KEKTVA 446
    Length:446
    Mass (Da):48,918
    Last modified:February 1, 1995 - v1
    Checksum:i1EC4CE1CE3BCD916
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti393 – 3931A → T in AAT93229. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77363 Genomic DNA. Translation: CAA54552.1.
    Z71665 Genomic DNA. Translation: CAA96331.1.
    AY693210 Genomic DNA. Translation: AAT93229.1.
    BK006947 Genomic DNA. Translation: DAA10591.1.
    PIRiS41937.
    RefSeqiNP_014448.1. NM_001183227.1.

    Genome annotation databases

    EnsemblFungiiYNR050C; YNR050C; YNR050C.
    GeneIDi855786.
    KEGGisce:YNR050C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77363 Genomic DNA. Translation: CAA54552.1 .
    Z71665 Genomic DNA. Translation: CAA96331.1 .
    AY693210 Genomic DNA. Translation: AAT93229.1 .
    BK006947 Genomic DNA. Translation: DAA10591.1 .
    PIRi S41937.
    RefSeqi NP_014448.1. NM_001183227.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2AXQ X-ray 1.70 A 1-446 [» ]
    ProteinModelPortali P38999.
    SMRi P38999. Positions 2-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35875. 14 interactions.
    DIPi DIP-776N.
    IntActi P38999. 9 interactions.
    MINTi MINT-425978.
    STRINGi 4932.YNR050C.

    Proteomic databases

    MaxQBi P38999.
    PaxDbi P38999.
    PeptideAtlasi P38999.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNR050C ; YNR050C ; YNR050C .
    GeneIDi 855786.
    KEGGi sce:YNR050C.

    Organism-specific databases

    CYGDi YNR050c.
    SGDi S000005333. LYS9.

    Phylogenomic databases

    eggNOGi COG1748.
    GeneTreei ENSGT00390000013249.
    HOGENOMi HOG000023158.
    KOi K00293.
    OMAi YAVKTIE.
    OrthoDBi EOG7FJH8S.

    Enzyme and pathway databases

    UniPathwayi UPA00033 ; UER00033 .
    BioCyci YEAST:MONOMER3O-363.

    Miscellaneous databases

    EvolutionaryTracei P38999.
    NextBioi 980265.

    Gene expression databases

    Genevestigatori P38999.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR016040. NAD(P)-bd_dom.
    IPR005097. Saccharopine_DH/HSpermid_syn.
    [Graphical view ]
    Pfami PF03435. Saccharop_dh. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Feller A.
      Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 1278B.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
      Norbeck J., Blomberg A.
      FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 314-324.
      Strain: ATCC 38531 / Y41.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA."
      Purushothaman S.K., Bujnicki J.M., Grosjean H., Lapeyre B.
      Mol. Cell. Biol. 25:4359-4370(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TRM112.

    Entry informationi

    Entry nameiLYS9_YEAST
    AccessioniPrimary (citable) accession number: P38999
    Secondary accession number(s): D6W1M5, E9P930
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 57500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3