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P38999 (LYS9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]

EC=1.5.1.10
Alternative name(s):
Saccharopine reductase
Gene names
Name:LYS9
Synonyms:LYS13
Ordered Locus Names:YNR050C
ORF Names:N3461
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.

Subunit structure

Interacts with TRM112. Ref.7

Miscellaneous

Present with 57500 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the saccharopine dehydrogenase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-10294,EBI-10294

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]
PRO_0000212839

Experimental info

Sequence conflict3931A → T in AAT93229. Ref.4

Secondary structure

............................................................................. 446
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38999 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 1EC4CE1CE3BCD916

FASTA44648,918
        10         20         30         40         50         60 
MGKNVLLLGS GFVAQPVIDT LAANDDINVT VACRTLANAQ ALAKPSGSKA ISLDVTDDSA 

        70         80         90        100        110        120 
LDKVLADNDV VISLIPYTFH PNVVKSAIRT KTDVVTSSYI SPALRELEPE IVKAGITVMN 

       130        140        150        160        170        180 
EIGLDPGIDH LYAVKTIDEV HRAGGKLKSF LSYCGGLPAP EDSDNPLGYK FSWSSRGVLL 

       190        200        210        220        230        240 
ALRNSAKYWK DGKIETVSSE DLMATAKPYF IYPGYAFVCY PNRDSTLFKD LYHIPEAETV 

       250        260        270        280        290        300 
IRGTLRYQGF PEFVKALVDM GMLKDDANEI FSKPIAWNEA LKQYLGAKST SKEDLIASID 

       310        320        330        340        350        360 
SKATWKDDED RERILSGFAW LGLFSDAKIT PRGNALDTLC ARLEELMQYE DNERDMVVLQ 

       370        380        390        400        410        420 
HKFGIEWADG TTETRTSTLV DYGKVGGYSS MAATVGYPVA IATKFVLDGT IKGPGLLAPY 

       430        440 
SPEINDPIMK ELKDKYGIYL KEKTVA 

« Hide

References

« Hide 'large scale' references
[1]Feller A.
Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1278B.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
Norbeck J., Blomberg A.
FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 314-324.
Strain: ATCC 38531 / Y41.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA."
Purushothaman S.K., Bujnicki J.M., Grosjean H., Lapeyre B.
Mol. Cell. Biol. 25:4359-4370(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TRM112.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77363 Genomic DNA. Translation: CAA54552.1.
Z71665 Genomic DNA. Translation: CAA96331.1.
AY693210 Genomic DNA. Translation: AAT93229.1.
BK006947 Genomic DNA. Translation: DAA10591.1.
PIRS41937.
RefSeqNP_014448.1. NM_001183227.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AXQX-ray1.70A1-446[»]
ProteinModelPortalP38999.
SMRP38999. Positions 2-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35875. 14 interactions.
DIPDIP-776N.
IntActP38999. 9 interactions.
MINTMINT-425978.
STRING4932.YNR050C.

Proteomic databases

MaxQBP38999.
PaxDbP38999.
PeptideAtlasP38999.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNR050C; YNR050C; YNR050C.
GeneID855786.
KEGGsce:YNR050C.

Organism-specific databases

CYGDYNR050c.
SGDS000005333. LYS9.

Phylogenomic databases

eggNOGCOG1748.
GeneTreeENSGT00390000013249.
HOGENOMHOG000023158.
KOK00293.
OMAYAVKTIE.
OrthoDBEOG7FJH8S.

Enzyme and pathway databases

BioCycYEAST:MONOMER3O-363.
UniPathwayUPA00033; UER00033.

Gene expression databases

GenevestigatorP38999.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR016040. NAD(P)-bd_dom.
IPR005097. Saccharopine_DH/HSpermid_syn.
[Graphical view]
PfamPF03435. Saccharop_dh. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38999.
NextBio980265.

Entry information

Entry nameLYS9_YEAST
AccessionPrimary (citable) accession number: P38999
Secondary accession number(s): D6W1M5, E9P930
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways