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P38994

- MSS4_YEAST

UniProt

P38994 - MSS4_YEAST

Protein

Probable phosphatidylinositol 4-phosphate 5-kinase MSS4

Gene

MSS4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate. Acts downstream of STT4, but in a pathway that does not involve PKC1. May be involved in the organization of the actin cytoskeleton.

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. ascospore-type prospore assembly Source: SGD
    2. phosphatidylinositol phosphorylation Source: SGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YDR208W-MONOMER.
    ReactomeiREACT_188986. Synthesis of PIPs at the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable phosphatidylinositol 4-phosphate 5-kinase MSS4 (EC:2.7.1.68)
    Alternative name(s):
    1-phosphatidylinositol 4-phosphate kinase
    Diphosphoinositide kinase
    PIP5K
    Gene namesi
    Name:MSS4
    Ordered Locus Names:YDR208W
    ORF Names:YD8142A.05
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR208w.
    SGDiS000002616. MSS4.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD
    2. plasma membrane Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 779779Probable phosphatidylinositol 4-phosphate 5-kinase MSS4PRO_0000185455Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei207 – 2071Phosphoserine1 Publication
    Modified residuei222 – 2221Phosphothreonine2 Publications
    Modified residuei225 – 2251Phosphoserine1 Publication
    Modified residuei659 – 6591Phosphothreonine2 Publications
    Modified residuei661 – 6611Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38994.
    PaxDbiP38994.
    PeptideAtlasiP38994.

    Expressioni

    Gene expression databases

    GenevestigatoriP38994.

    Interactioni

    Protein-protein interaction databases

    BioGridi32258. 84 interactions.
    IntActiP38994. 3 interactions.
    MINTiMINT-2731747.
    STRINGi4932.YDR208W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38994.
    SMRiP38994. Positions 392-752.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini376 – 756381PIPKPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PIPK domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5253.
    GeneTreeiENSGT00690000101870.
    HOGENOMiHOG000193875.
    KOiK00889.
    OMAiHSEHIHL.
    OrthoDBiEOG7XSTPB.

    Family and domain databases

    Gene3Di3.30.800.10. 1 hit.
    3.30.810.10. 1 hit.
    InterProiIPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view]
    PANTHERiPTHR23086. PTHR23086. 1 hit.
    PfamiPF01504. PIP5K. 1 hit.
    [Graphical view]
    SMARTiSM00330. PIPKc. 1 hit.
    [Graphical view]
    PROSITEiPS51455. PIPK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38994-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVLRSQPPS VVPLHLTTST SRKTEQEPSL LHSAIIERHQ DRSVPNSNSN    50
    PDSNHRIKKD RNNHTSYHSS SNSESNMESP RLSDGESSTP TSIEELNPTI 100
    NNSRLVKRNY SISIDPLHDN SNNNTDDDHP NTITSPRPNS TSNKEMQKYS 150
    FPEGKESKKI TTPSLNSNNC LDLDNSSLVH TDSYIQDLND DHILLNKRVS 200
    RRSSRISAVT ATSTTIKQRR NTQDSNLPNI PFHASKHSQI LPMDDSDVIK 250
    LANGDTSMKP NSATKISHSM TSLPLHPLPQ PSQKSKQYHM ISKSTTSLPP 300
    ENDHYYQHSR GTNHNHAANA AAVNNNTTTT TAATGLKRSE SATAEIKKMR 350
    QSLLHKREMK RKRKTFLVDD DRVLIGNKVS EGHVNFIIAY NMLTGIRVAV 400
    SRCSGIMKPL TPADFRFTKK LAFDYHGNEL TPSSQYAFKF KDYCPEVFRE 450
    LRALFGLDPA DYLVSLTSKY ILSELNSPGK SGSFFYYSRD YKYIIKTIHH 500
    SEHIHLRKHI QEYYNHVRDN PNTLICQFYG LHRVKMPISF QNKIKHRKIY 550
    FLVMNNLFPP HLDIHITYDL KGSTWGRFTN LDKERLAKDR SYRPVMKDLN 600
    WLEEGQKIKF GPLKKKTFLT QLKKDVELLA KLNTMDYSLL IGIHDINKAK 650
    EDDLQLADTA SIEEQPQTQG PIRTGTGTVV RHFFREFEGG IRASDQFNND 700
    VDLIYYVGII DFLTNYSVMK KLETFWRSLR HDTKLVSAIP PRDYANRFYE 750
    FIEDSVDPLP QKKTQSSYRD DPNQKNYKD 779
    Length:779
    Mass (Da):89,320
    Last modified:October 1, 1996 - v2
    Checksum:iF66B796229CFB8F6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti610 – 6101F → L in BAA02869. (PubMed:8152413)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13716 Genomic DNA. Translation: BAA02869.1.
    Z68194 Genomic DNA. Translation: CAA92347.1.
    BK006938 Genomic DNA. Translation: DAA12049.1.
    PIRiS61571.
    RefSeqiNP_010494.1. NM_001180516.1.

    Genome annotation databases

    EnsemblFungiiYDR208W; YDR208W; YDR208W.
    GeneIDi851789.
    KEGGisce:YDR208W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13716 Genomic DNA. Translation: BAA02869.1 .
    Z68194 Genomic DNA. Translation: CAA92347.1 .
    BK006938 Genomic DNA. Translation: DAA12049.1 .
    PIRi S61571.
    RefSeqi NP_010494.1. NM_001180516.1.

    3D structure databases

    ProteinModelPortali P38994.
    SMRi P38994. Positions 392-752.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32258. 84 interactions.
    IntActi P38994. 3 interactions.
    MINTi MINT-2731747.
    STRINGi 4932.YDR208W.

    Proteomic databases

    MaxQBi P38994.
    PaxDbi P38994.
    PeptideAtlasi P38994.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR208W ; YDR208W ; YDR208W .
    GeneIDi 851789.
    KEGGi sce:YDR208W.

    Organism-specific databases

    CYGDi YDR208w.
    SGDi S000002616. MSS4.

    Phylogenomic databases

    eggNOGi COG5253.
    GeneTreei ENSGT00690000101870.
    HOGENOMi HOG000193875.
    KOi K00889.
    OMAi HSEHIHL.
    OrthoDBi EOG7XSTPB.

    Enzyme and pathway databases

    BioCyci YEAST:YDR208W-MONOMER.
    Reactomei REACT_188986. Synthesis of PIPs at the plasma membrane.

    Miscellaneous databases

    NextBioi 969609.

    Gene expression databases

    Genevestigatori P38994.

    Family and domain databases

    Gene3Di 3.30.800.10. 1 hit.
    3.30.810.10. 1 hit.
    InterProi IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view ]
    PANTHERi PTHR23086. PTHR23086. 1 hit.
    Pfami PF01504. PIP5K. 1 hit.
    [Graphical view ]
    SMARTi SM00330. PIPKc. 1 hit.
    [Graphical view ]
    PROSITEi PS51455. PIPK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae."
      Yoshida S., Ohya Y., Nakano A., Anraku Y.
      Mol. Gen. Genet. 242:631-640(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "MSS4, a phosphatidylinositol-4-phosphate 5-kinase required for organization of the actin cytoskeleton in Saccharomyces cerevisiae."
      Desrivieres S., Cooke F.T., Parker P.J., Hall M.N.
      J. Biol. Chem. 273:15787-15793(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-222, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-659 AND SER-661, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207; THR-222; SER-225; THR-659 AND SER-661, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMSS4_YEAST
    AccessioniPrimary (citable) accession number: P38994
    Secondary accession number(s): D6VSI9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3