SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P38994

- MSS4_YEAST

UniProt

P38994 - MSS4_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Probable phosphatidylinositol 4-phosphate 5-kinase MSS4

Gene
MSS4, YDR208W, YD8142A.05
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate. Acts downstream of STT4, but in a pathway that does not involve PKC1. May be involved in the organization of the actin cytoskeleton.

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

GO - Molecular functioni

  1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: SGD
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. ascospore-type prospore assembly Source: SGD
  2. phosphatidylinositol phosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YDR208W-MONOMER.
ReactomeiREACT_188986. Synthesis of PIPs at the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphatidylinositol 4-phosphate 5-kinase MSS4 (EC:2.7.1.68)
Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase
Diphosphoinositide kinase
PIP5K
Gene namesi
Name:MSS4
Ordered Locus Names:YDR208W
ORF Names:YD8142A.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR208w.
SGDiS000002616. MSS4.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
  2. plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 779779Probable phosphatidylinositol 4-phosphate 5-kinase MSS4PRO_0000185455Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei207 – 2071Phosphoserine1 Publication
Modified residuei222 – 2221Phosphothreonine2 Publications
Modified residuei225 – 2251Phosphoserine1 Publication
Modified residuei659 – 6591Phosphothreonine2 Publications
Modified residuei661 – 6611Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38994.
PaxDbiP38994.
PeptideAtlasiP38994.

Expressioni

Gene expression databases

GenevestigatoriP38994.

Interactioni

Protein-protein interaction databases

BioGridi32258. 84 interactions.
IntActiP38994. 3 interactions.
MINTiMINT-2731747.
STRINGi4932.YDR208W.

Structurei

3D structure databases

ProteinModelPortaliP38994.
SMRiP38994. Positions 392-752.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini376 – 756381PIPKAdd
BLAST

Sequence similaritiesi

Contains 1 PIPK domain.

Phylogenomic databases

eggNOGiCOG5253.
GeneTreeiENSGT00690000101870.
HOGENOMiHOG000193875.
KOiK00889.
OMAiHSEHIHL.
OrthoDBiEOG7XSTPB.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 1 hit.
InterProiIPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38994-1 [UniParc]FASTAAdd to Basket

« Hide

MSVLRSQPPS VVPLHLTTST SRKTEQEPSL LHSAIIERHQ DRSVPNSNSN    50
PDSNHRIKKD RNNHTSYHSS SNSESNMESP RLSDGESSTP TSIEELNPTI 100
NNSRLVKRNY SISIDPLHDN SNNNTDDDHP NTITSPRPNS TSNKEMQKYS 150
FPEGKESKKI TTPSLNSNNC LDLDNSSLVH TDSYIQDLND DHILLNKRVS 200
RRSSRISAVT ATSTTIKQRR NTQDSNLPNI PFHASKHSQI LPMDDSDVIK 250
LANGDTSMKP NSATKISHSM TSLPLHPLPQ PSQKSKQYHM ISKSTTSLPP 300
ENDHYYQHSR GTNHNHAANA AAVNNNTTTT TAATGLKRSE SATAEIKKMR 350
QSLLHKREMK RKRKTFLVDD DRVLIGNKVS EGHVNFIIAY NMLTGIRVAV 400
SRCSGIMKPL TPADFRFTKK LAFDYHGNEL TPSSQYAFKF KDYCPEVFRE 450
LRALFGLDPA DYLVSLTSKY ILSELNSPGK SGSFFYYSRD YKYIIKTIHH 500
SEHIHLRKHI QEYYNHVRDN PNTLICQFYG LHRVKMPISF QNKIKHRKIY 550
FLVMNNLFPP HLDIHITYDL KGSTWGRFTN LDKERLAKDR SYRPVMKDLN 600
WLEEGQKIKF GPLKKKTFLT QLKKDVELLA KLNTMDYSLL IGIHDINKAK 650
EDDLQLADTA SIEEQPQTQG PIRTGTGTVV RHFFREFEGG IRASDQFNND 700
VDLIYYVGII DFLTNYSVMK KLETFWRSLR HDTKLVSAIP PRDYANRFYE 750
FIEDSVDPLP QKKTQSSYRD DPNQKNYKD 779
Length:779
Mass (Da):89,320
Last modified:October 1, 1996 - v2
Checksum:iF66B796229CFB8F6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti610 – 6101F → L in BAA02869. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13716 Genomic DNA. Translation: BAA02869.1.
Z68194 Genomic DNA. Translation: CAA92347.1.
BK006938 Genomic DNA. Translation: DAA12049.1.
PIRiS61571.
RefSeqiNP_010494.1. NM_001180516.1.

Genome annotation databases

EnsemblFungiiYDR208W; YDR208W; YDR208W.
GeneIDi851789.
KEGGisce:YDR208W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13716 Genomic DNA. Translation: BAA02869.1 .
Z68194 Genomic DNA. Translation: CAA92347.1 .
BK006938 Genomic DNA. Translation: DAA12049.1 .
PIRi S61571.
RefSeqi NP_010494.1. NM_001180516.1.

3D structure databases

ProteinModelPortali P38994.
SMRi P38994. Positions 392-752.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32258. 84 interactions.
IntActi P38994. 3 interactions.
MINTi MINT-2731747.
STRINGi 4932.YDR208W.

Proteomic databases

MaxQBi P38994.
PaxDbi P38994.
PeptideAtlasi P38994.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR208W ; YDR208W ; YDR208W .
GeneIDi 851789.
KEGGi sce:YDR208W.

Organism-specific databases

CYGDi YDR208w.
SGDi S000002616. MSS4.

Phylogenomic databases

eggNOGi COG5253.
GeneTreei ENSGT00690000101870.
HOGENOMi HOG000193875.
KOi K00889.
OMAi HSEHIHL.
OrthoDBi EOG7XSTPB.

Enzyme and pathway databases

BioCyci YEAST:YDR208W-MONOMER.
Reactomei REACT_188986. Synthesis of PIPs at the plasma membrane.

Miscellaneous databases

NextBioi 969609.

Gene expression databases

Genevestigatori P38994.

Family and domain databases

Gene3Di 3.30.800.10. 1 hit.
3.30.810.10. 1 hit.
InterProi IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view ]
PANTHERi PTHR23086. PTHR23086. 1 hit.
Pfami PF01504. PIP5K. 1 hit.
[Graphical view ]
SMARTi SM00330. PIPKc. 1 hit.
[Graphical view ]
PROSITEi PS51455. PIPK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae."
    Yoshida S., Ohya Y., Nakano A., Anraku Y.
    Mol. Gen. Genet. 242:631-640(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "MSS4, a phosphatidylinositol-4-phosphate 5-kinase required for organization of the actin cytoskeleton in Saccharomyces cerevisiae."
    Desrivieres S., Cooke F.T., Parker P.J., Hall M.N.
    J. Biol. Chem. 273:15787-15793(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-222, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-659 AND SER-661, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207; THR-222; SER-225; THR-659 AND SER-661, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMSS4_YEAST
AccessioniPrimary (citable) accession number: P38994
Secondary accession number(s): D6VSI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi