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P38994 (MSS4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable phosphatidylinositol 4-phosphate 5-kinase MSS4
EC=2.7.1.68
Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase
Diphosphoinositide kinase
PIP5K
Gene names
Name:MSS4
Ordered Locus Names:YDR208W
ORF Names:YD8142A.05
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length779 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate. Acts downstream of STT4, but in a pathway that does not involve PKC1. May be involved in the organization of the actin cytoskeleton.

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Sequence similarities

Contains 1 PIPK domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 779779Probable phosphatidylinositol 4-phosphate 5-kinase MSS4
PRO_0000185455

Regions

Domain376 – 756381PIPK

Amino acid modifications

Modified residue2221Phosphothreonine Ref.5
Modified residue2691Phosphoserine Ref.7
Modified residue6591Phosphothreonine Ref.6 Ref.7
Modified residue6611Phosphoserine Ref.6 Ref.7

Experimental info

Sequence conflict6101F → L in BAA02869. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P38994 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: F66B796229CFB8F6

FASTA77989,320
        10         20         30         40         50         60 
MSVLRSQPPS VVPLHLTTST SRKTEQEPSL LHSAIIERHQ DRSVPNSNSN PDSNHRIKKD 

        70         80         90        100        110        120 
RNNHTSYHSS SNSESNMESP RLSDGESSTP TSIEELNPTI NNSRLVKRNY SISIDPLHDN 

       130        140        150        160        170        180 
SNNNTDDDHP NTITSPRPNS TSNKEMQKYS FPEGKESKKI TTPSLNSNNC LDLDNSSLVH 

       190        200        210        220        230        240 
TDSYIQDLND DHILLNKRVS RRSSRISAVT ATSTTIKQRR NTQDSNLPNI PFHASKHSQI 

       250        260        270        280        290        300 
LPMDDSDVIK LANGDTSMKP NSATKISHSM TSLPLHPLPQ PSQKSKQYHM ISKSTTSLPP 

       310        320        330        340        350        360 
ENDHYYQHSR GTNHNHAANA AAVNNNTTTT TAATGLKRSE SATAEIKKMR QSLLHKREMK 

       370        380        390        400        410        420 
RKRKTFLVDD DRVLIGNKVS EGHVNFIIAY NMLTGIRVAV SRCSGIMKPL TPADFRFTKK 

       430        440        450        460        470        480 
LAFDYHGNEL TPSSQYAFKF KDYCPEVFRE LRALFGLDPA DYLVSLTSKY ILSELNSPGK 

       490        500        510        520        530        540 
SGSFFYYSRD YKYIIKTIHH SEHIHLRKHI QEYYNHVRDN PNTLICQFYG LHRVKMPISF 

       550        560        570        580        590        600 
QNKIKHRKIY FLVMNNLFPP HLDIHITYDL KGSTWGRFTN LDKERLAKDR SYRPVMKDLN 

       610        620        630        640        650        660 
WLEEGQKIKF GPLKKKTFLT QLKKDVELLA KLNTMDYSLL IGIHDINKAK EDDLQLADTA 

       670        680        690        700        710        720 
SIEEQPQTQG PIRTGTGTVV RHFFREFEGG IRASDQFNND VDLIYYVGII DFLTNYSVMK 

       730        740        750        760        770 
KLETFWRSLR HDTKLVSAIP PRDYANRFYE FIEDSVDPLP QKKTQSSYRD DPNQKNYKD

« Hide

References

« Hide 'large scale' references
[1]"Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae."
Yoshida S., Ohya Y., Nakano A., Anraku Y.
Mol. Gen. Genet. 242:631-640(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"MSS4, a phosphatidylinositol-4-phosphate 5-kinase required for organization of the actin cytoskeleton in Saccharomyces cerevisiae."
Desrivieres S., Cooke F.T., Parker P.J., Hall M.N.
J. Biol. Chem. 273:15787-15793(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-222, MASS SPECTROMETRY.
Strain: ADR376.
[6]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-659 AND SER-661, MASS SPECTROMETRY.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269; THR-659 AND SER-661, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D13716 Genomic DNA. Translation: BAA02869.1.
Z68194 Genomic DNA. Translation: CAA92347.1.
BK006938 Genomic DNA. Translation: DAA12049.1.
PIRS61571.
RefSeqNP_010494.1. NM_001180516.1.

3D structure databases

ProteinModelPortalP38994.
SMRP38994. Positions 392-752.
ModBaseSearch...

Protein-protein interaction databases

IntActP38994. 2 interactions.
MINTMINT-2731747.
STRING4932.YDR208W.

Proteomic databases

PaxDbP38994.
PeptideAtlasP38994.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR208W; YDR208W; YDR208W.
GeneID851789.
KEGGsce:YDR208W.

Organism-specific databases

CYGDYDR208w.
SGDS000002616. MSS4.

Phylogenomic databases

eggNOGCOG5253.
GeneTreeENSGT00690000101870.
HOGENOMHOG000193875.
KOK00889.
OMAAIPPRDY.
OrthoDBEOG4H49CG.

Gene expression databases

GenevestigatorP38994.
GermOnlineYDR208W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR023610. PInositol-4-P-5-kinase.
IPR002498. PInositol-4-P-5-kinase_core.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERPTHR23086. PTHR23086. 1 hit.
PfamPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio969609.

Entry information

Entry nameMSS4_YEAST
AccessionPrimary (citable) accession number: P38994
Secondary accession number(s): D6VSI9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents