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Protein

Probable phosphatidylinositol 4-phosphate 5-kinase MSS4

Gene

MSS4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate. Acts downstream of STT4, but in a pathway that does not involve PKC1. May be involved in the organization of the actin cytoskeleton.

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

GO - Molecular functioni

  • 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: SGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • ascospore-type prospore assembly Source: SGD
  • cortical actin cytoskeleton organization Source: CACAO
  • phosphatidylinositol phosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YDR208W-MONOMER.
ReactomeiR-SCE-1660499. Synthesis of PIPs at the plasma membrane.
R-SCE-6811555. PI5P Regulates TP53 Acetylation.
R-SCE-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-SCE-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphatidylinositol 4-phosphate 5-kinase MSS4 (EC:2.7.1.68)
Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase
Diphosphoinositide kinase
PIP5K
Gene namesi
Name:MSS4
Ordered Locus Names:YDR208W
ORF Names:YD8142A.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR208W.
SGDiS000002616. MSS4.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001854551 – 779Probable phosphatidylinositol 4-phosphate 5-kinase MSS4Add BLAST779

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei207PhosphoserineCombined sources1
Modified residuei222PhosphothreonineCombined sources1
Modified residuei225PhosphoserineCombined sources1
Modified residuei659PhosphothreonineCombined sources1
Modified residuei661PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38994.
PRIDEiP38994.

PTM databases

iPTMnetiP38994.

Interactioni

Protein-protein interaction databases

BioGridi32258. 86 interactors.
IntActiP38994. 3 interactors.
MINTiMINT-2731747.

Structurei

3D structure databases

ProteinModelPortaliP38994.
SMRiP38994.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini376 – 756PIPKPROSITE-ProRule annotationAdd BLAST381

Sequence similaritiesi

Contains 1 PIPK domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119184.
HOGENOMiHOG000193875.
InParanoidiP38994.
KOiK00889.
OMAiFYEFIED.
OrthoDBiEOG092C4AR2.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 1 hit.
InterProiIPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38994-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVLRSQPPS VVPLHLTTST SRKTEQEPSL LHSAIIERHQ DRSVPNSNSN
60 70 80 90 100
PDSNHRIKKD RNNHTSYHSS SNSESNMESP RLSDGESSTP TSIEELNPTI
110 120 130 140 150
NNSRLVKRNY SISIDPLHDN SNNNTDDDHP NTITSPRPNS TSNKEMQKYS
160 170 180 190 200
FPEGKESKKI TTPSLNSNNC LDLDNSSLVH TDSYIQDLND DHILLNKRVS
210 220 230 240 250
RRSSRISAVT ATSTTIKQRR NTQDSNLPNI PFHASKHSQI LPMDDSDVIK
260 270 280 290 300
LANGDTSMKP NSATKISHSM TSLPLHPLPQ PSQKSKQYHM ISKSTTSLPP
310 320 330 340 350
ENDHYYQHSR GTNHNHAANA AAVNNNTTTT TAATGLKRSE SATAEIKKMR
360 370 380 390 400
QSLLHKREMK RKRKTFLVDD DRVLIGNKVS EGHVNFIIAY NMLTGIRVAV
410 420 430 440 450
SRCSGIMKPL TPADFRFTKK LAFDYHGNEL TPSSQYAFKF KDYCPEVFRE
460 470 480 490 500
LRALFGLDPA DYLVSLTSKY ILSELNSPGK SGSFFYYSRD YKYIIKTIHH
510 520 530 540 550
SEHIHLRKHI QEYYNHVRDN PNTLICQFYG LHRVKMPISF QNKIKHRKIY
560 570 580 590 600
FLVMNNLFPP HLDIHITYDL KGSTWGRFTN LDKERLAKDR SYRPVMKDLN
610 620 630 640 650
WLEEGQKIKF GPLKKKTFLT QLKKDVELLA KLNTMDYSLL IGIHDINKAK
660 670 680 690 700
EDDLQLADTA SIEEQPQTQG PIRTGTGTVV RHFFREFEGG IRASDQFNND
710 720 730 740 750
VDLIYYVGII DFLTNYSVMK KLETFWRSLR HDTKLVSAIP PRDYANRFYE
760 770
FIEDSVDPLP QKKTQSSYRD DPNQKNYKD
Length:779
Mass (Da):89,320
Last modified:October 1, 1996 - v2
Checksum:iF66B796229CFB8F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti610F → L in BAA02869 (PubMed:8152413).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13716 Genomic DNA. Translation: BAA02869.1.
Z68194 Genomic DNA. Translation: CAA92347.1.
BK006938 Genomic DNA. Translation: DAA12049.1.
PIRiS61571.
RefSeqiNP_010494.1. NM_001180516.1.

Genome annotation databases

EnsemblFungiiYDR208W; YDR208W; YDR208W.
GeneIDi851789.
KEGGisce:YDR208W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13716 Genomic DNA. Translation: BAA02869.1.
Z68194 Genomic DNA. Translation: CAA92347.1.
BK006938 Genomic DNA. Translation: DAA12049.1.
PIRiS61571.
RefSeqiNP_010494.1. NM_001180516.1.

3D structure databases

ProteinModelPortaliP38994.
SMRiP38994.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32258. 86 interactors.
IntActiP38994. 3 interactors.
MINTiMINT-2731747.

PTM databases

iPTMnetiP38994.

Proteomic databases

MaxQBiP38994.
PRIDEiP38994.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR208W; YDR208W; YDR208W.
GeneIDi851789.
KEGGisce:YDR208W.

Organism-specific databases

EuPathDBiFungiDB:YDR208W.
SGDiS000002616. MSS4.

Phylogenomic databases

GeneTreeiENSGT00760000119184.
HOGENOMiHOG000193875.
InParanoidiP38994.
KOiK00889.
OMAiFYEFIED.
OrthoDBiEOG092C4AR2.

Enzyme and pathway databases

BioCyciYEAST:YDR208W-MONOMER.
ReactomeiR-SCE-1660499. Synthesis of PIPs at the plasma membrane.
R-SCE-6811555. PI5P Regulates TP53 Acetylation.
R-SCE-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-SCE-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiP38994.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 1 hit.
InterProiIPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSS4_YEAST
AccessioniPrimary (citable) accession number: P38994
Secondary accession number(s): D6VSI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.