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Protein

Iron transport multicopper oxidase FET3

Gene

FET3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron transport multicopper ferroxidase required for Fe2+ ion high affinity uptake. Required to oxidize Fe2+ and release it from the transporter. Essential component of copper-dependent iron transport.

Cofactori

Cu cationNote: Binds 4 Cu cations per monomer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi81Copper 1; type 21
Metal bindingi83Copper 2; type 31
Metal bindingi126Copper 2; type 31
Metal bindingi128Copper 3; type 31
Metal bindingi413Copper 4; type 11
Metal bindingi416Copper 1; type 21
Metal bindingi418Copper 3; type 31
Metal bindingi483Copper 3; type 31
Metal bindingi484Copper 4; type 11
Metal bindingi485Copper 2; type 31
Metal bindingi489Copper 4; type 11

GO - Molecular functioni

  • copper ion binding Source: GO_Central
  • ferroxidase activity Source: SGD

GO - Biological processi

  • arsenate ion transmembrane transport Source: SGD
  • high-affinity iron ion transmembrane transport Source: SGD
  • iron assimilation by reduction and transport Source: SGD
  • response to copper ion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Copper, Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YMR058W-MONOMER.
SABIO-RKP38993.

Protein family/group databases

TCDBi2.A.108.1.1. the iron/lead transporter (ilt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron transport multicopper oxidase FET3 (EC:1.-.-.-)
Gene namesi
Name:FET3
Ordered Locus Names:YMR058W
ORF Names:YM9796.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR058W.
SGDiS000004662. FET3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 559ExtracellularSequence analysisAdd BLAST538
Transmembranei560 – 584HelicalSequence analysisAdd BLAST25
Topological domaini585 – 636CytoplasmicSequence analysisAdd BLAST52

GO - Cellular componenti

  • high-affinity iron permease complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000295922 – 636Iron transport multicopper oxidase FET3Add BLAST615

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...)1 Publication1
Glycosylationi74N-linked (GlcNAc...)Sequence analysis1
Glycosylationi77N-linked (GlcNAc...)1 Publication1
Glycosylationi88N-linked (GlcNAc...)1 Publication1
Glycosylationi113N-linked (GlcNAc...)1 Publication1
Glycosylationi194N-linked (GlcNAc...)1 Publication1
Glycosylationi198N-linked (GlcNAc...)1 Publication1
Glycosylationi244N-linked (GlcNAc...)1 Publication1
Glycosylationi265N-linked (GlcNAc...)Sequence analysis1
Glycosylationi292N-linked (GlcNAc...)1
Glycosylationi300N-linked (GlcNAc...)1 Publication1
Glycosylationi359N-linked (GlcNAc...)1 Publication1
Glycosylationi381N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP38993.
PRIDEiP38993.

PTM databases

iPTMnetiP38993.

Expressioni

Inductioni

By iron deprivation. Repressed by iron excess.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FTR1P400886EBI-6876,EBI-7138

Protein-protein interaction databases

BioGridi35233. 93 interactors.
DIPiDIP-5314N.
IntActiP38993. 3 interactors.
MINTiMINT-550704.

Structurei

Secondary structure

1636
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 35Combined sources13
Beta strandi37 – 40Combined sources4
Beta strandi43 – 48Combined sources6
Beta strandi51 – 53Combined sources3
Beta strandi57 – 60Combined sources4
Beta strandi64 – 70Combined sources7
Beta strandi81 – 83Combined sources3
Helixi91 – 93Combined sources3
Turni97 – 99Combined sources3
Beta strandi109 – 115Combined sources7
Beta strandi121 – 126Combined sources6
Beta strandi129 – 131Combined sources3
Helixi132 – 135Combined sources4
Beta strandi138 – 144Combined sources7
Beta strandi153 – 163Combined sources11
Helixi168 – 175Combined sources8
Beta strandi189 – 193Combined sources5
Beta strandi196 – 198Combined sources3
Beta strandi200 – 202Combined sources3
Beta strandi208 – 215Combined sources8
Beta strandi222 – 226Combined sources5
Beta strandi231 – 236Combined sources6
Beta strandi239 – 247Combined sources9
Beta strandi249 – 251Combined sources3
Beta strandi256 – 262Combined sources7
Beta strandi271 – 277Combined sources7
Helixi279 – 281Combined sources3
Beta strandi282 – 284Combined sources3
Beta strandi292 – 299Combined sources8
Helixi320 – 322Combined sources3
Beta strandi325 – 327Combined sources3
Beta strandi336 – 347Combined sources12
Beta strandi353 – 357Combined sources5
Helixi369 – 373Combined sources5
Helixi377 – 379Combined sources3
Helixi383 – 386Combined sources4
Beta strandi388 – 390Combined sources3
Beta strandi392 – 395Combined sources4
Beta strandi400 – 407Combined sources8
Beta strandi409 – 411Combined sources3
Beta strandi413 – 417Combined sources5
Beta strandi422 – 427Combined sources6
Helixi433 – 435Combined sources3
Beta strandi455 – 462Combined sources8
Beta strandi467 – 473Combined sources7
Beta strandi478 – 484Combined sources7
Helixi487 – 491Combined sources5
Beta strandi495 – 500Combined sources6
Helixi502 – 507Combined sources6
Helixi509 – 511Combined sources3
Helixi515 – 523Combined sources9
Beta strandi529 – 533Combined sources5
Beta strandi535 – 537Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZPUX-ray2.80A/B/C/D/E/F22-555[»]
ProteinModelPortaliP38993.
SMRiP38993.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38993.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 144Plastocyanin-like 1Add BLAST72
Domaini190 – 300Plastocyanin-like 2Add BLAST111
Domaini382 – 500Plastocyanin-like 3Add BLAST119

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000064508.
HOGENOMiHOG000159793.
InParanoidiP38993.
KOiK19791.
OrthoDBiEOG092C10OL.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 2 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38993-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNALLSIAV LLFSMLSLAQ AETHTFNWTT GWDYRNVDGL KSRPVITCNG
60 70 80 90 100
QFPWPDITVN KGDRVQIYLT NGMNNTNTSM HFHGLFQNGT ASMDGVPFLT
110 120 130 140 150
QCPIAPGSTM LYNFTVDYNV GTYWYHSHTD GQYEDGMKGL FIIKDDSFPY
160 170 180 190 200
DYDEELSLSL SEWYHDLVTD LTKSFMSVYN PTGAEPIPQN LIVNNTMNLT
210 220 230 240 250
WEVQPDTTYL LRIVNVGGFV SQYFWIEDHE MTVVEIDGIT TEKNVTDMLY
260 270 280 290 300
ITVAQRYTVL VHTKNDTDKN FAIMQKFDDT MLDVIPSDLQ LNATSYMVYN
310 320 330 340 350
KTAALPTQNY VDSIDNFLDD FYLQPYEKEA IYGEPDHVIT VDVVMDNLKN
360 370 380 390 400
GVNYAFFNNI TYTAPKVPTL MTVLSSGDQA NNSEIYGSNT HTFILEKDEI
410 420 430 440 450
VEIVLNNQDT GTHPFHLHGH AFQTIQRDRT YDDALGEVPH SFDPDNHPAF
460 470 480 490 500
PEYPMRRDTL YVRPQSNFVI RFKADNPGVW FFHCHIEWHL LQGLGLVLVE
510 520 530 540 550
DPFGIQDAHS QQLSENHLEV CQSCSVATEG NAAANTLDLT DLTGENVQHA
560 570 580 590 600
FIPTGFTKKG IIAMTFSCFA GILGIITIAI YGMMDMEDAT EKVIRDLHVD
610 620 630
PEVLLNEVDE NEERQVNEDR HSTEKHQFLT KAKRFF
Length:636
Mass (Da):72,360
Last modified:November 1, 1997 - v2
Checksum:i71CB4352B87746E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25090 Genomic DNA. Translation: AAA64929.1.
Z49703 Genomic DNA. Translation: CAA89768.1.
BK006946 Genomic DNA. Translation: DAA09956.1.
PIRiA55428.
RefSeqiNP_013774.1. NM_001182556.1.

Genome annotation databases

EnsemblFungiiYMR058W; YMR058W; YMR058W.
GeneIDi855080.
KEGGisce:YMR058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25090 Genomic DNA. Translation: AAA64929.1.
Z49703 Genomic DNA. Translation: CAA89768.1.
BK006946 Genomic DNA. Translation: DAA09956.1.
PIRiA55428.
RefSeqiNP_013774.1. NM_001182556.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZPUX-ray2.80A/B/C/D/E/F22-555[»]
ProteinModelPortaliP38993.
SMRiP38993.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35233. 93 interactors.
DIPiDIP-5314N.
IntActiP38993. 3 interactors.
MINTiMINT-550704.

Protein family/group databases

TCDBi2.A.108.1.1. the iron/lead transporter (ilt) family.

PTM databases

iPTMnetiP38993.

Proteomic databases

MaxQBiP38993.
PRIDEiP38993.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR058W; YMR058W; YMR058W.
GeneIDi855080.
KEGGisce:YMR058W.

Organism-specific databases

EuPathDBiFungiDB:YMR058W.
SGDiS000004662. FET3.

Phylogenomic databases

GeneTreeiENSGT00530000064508.
HOGENOMiHOG000159793.
InParanoidiP38993.
KOiK19791.
OrthoDBiEOG092C10OL.

Enzyme and pathway databases

BioCyciYEAST:YMR058W-MONOMER.
SABIO-RKP38993.

Miscellaneous databases

EvolutionaryTraceiP38993.
PROiP38993.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 2 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFET3_YEAST
AccessioniPrimary (citable) accession number: P38993
Secondary accession number(s): D6VZN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1050 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.