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Protein

Spindle assembly checkpoint kinase

Gene

IPL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for high-fidelity chromosome segregation during the later part of each cell cycle. Acts in opposition to the phosphatase PP1. Has a role in attaching the kinetochores to the microtubules and ensuring that sister kinetochores connect to opposite poles. The promotion of bi-orientation is achieved by selectively detaching kinetochore-microtubule attachments that are not under tension. Phosphorylates histone H3 to form H3S10ph during mitosis and meiosis. Phosphorylates RGD1 in vitro.4 Publications

Miscellaneous

Present with 149 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei133ATPPROSITE-ProRule annotation1
Active sitei227Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi110 – 118ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • attachment of spindle microtubules to kinetochore Source: SGD
  • chromosome segregation Source: SGD
  • homologous chromosome segregation Source: SGD
  • meiotic sister chromatid segregation Source: SGD
  • mitotic DNA integrity checkpoint Source: SGD
  • mitotic spindle disassembly Source: SGD
  • negative regulation of protein import into nucleus during spindle assembly checkpoint Source: SGD
  • peptidyl-serine phosphorylation Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of cytokinesis Source: SGD
  • regulation of mitotic cell cycle spindle assembly checkpoint Source: SGD
  • regulation of mitotic spindle assembly Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Chromosome partition
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34100-MONOMER
BRENDAi2.7.11.1 984
ReactomeiR-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Spindle assembly checkpoint kinase (EC:2.7.11.1)
Alternative name(s):
Aurora kinase
Increase-in-ploidy protein 1
Gene namesi
Name:IPL1
Ordered Locus Names:YPL209C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL209C
SGDiS000006130 IPL1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi260T → A in IPL1-4; causes missegregation of chromosomes at 37 degrees Celsius. 1 Publication1
Mutagenesisi340P → L in IPL1-1; causes missegregation of chromosomes at 37 degrees Celsius. 1 Publication1
Mutagenesisi352H → Y in IPL1-2; causes missegregation of chromosomes at 37 degrees Celsius. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860291 – 367Spindle assembly checkpoint kinaseAdd BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5Phosphoserine; by autocatalysis1 Publication1
Modified residuei76Phosphoserine1 Publication1
Modified residuei260Phosphothreonine; by autocatalysis1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38991
PaxDbiP38991
PRIDEiP38991

PTM databases

iPTMnetiP38991

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35976, 499 interactors
DIPiDIP-2771N
IntActiP38991, 11 interactors
MINTiP38991
STRINGi4932.YPL209C

Structurei

3D structure databases

ProteinModelPortaliP38991
SMRiP38991
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 355Protein kinasePROSITE-ProRule annotationAdd BLAST252

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00910000144066
HOGENOMiHOG000233016
InParanoidiP38991
KOiK08850
OMAiQATSVPH
OrthoDBiEOG092C0QJU

Family and domain databases

InterProiView protein in InterPro
IPR030616 Aur
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR24350 PTHR24350, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P38991-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRNSLVNIK LNANSPSKKT TTRPNTSRIN KPWRISHSPQ QRNPNSKIPS
60 70 80 90 100
PVREKLNRLP VNNKKFLDME SSKIPSPIRK ATSSKMIHEN KKLPKFKSLS
110 120 130 140 150
LDDFELGKKL GKGKFGKVYC VRHRSTGYIC ALKVMEKEEI IKYNLQKQFR
160 170 180 190 200
REVEIQTSLN HPNLTKSYGY FHDEKRVYLL MEYLVNGEMY KLLRLHGPFN
210 220 230 240 250
DILASDYIYQ IANALDYMHK KNIIHRDIKP ENILIGFNNV IKLTDFGWSI
260 270 280 290 300
INPPENRRKT VCGTIDYLSP EMVESREYDH TIDAWALGVL AFELLTGAPP
310 320 330 340 350
FEEEMKDTTY KRIAALDIKM PSNISQDAQD LILKLLKYDP KDRMRLGDVK
360
MHPWILRNKP FWENKRL
Length:367
Mass (Da):42,946
Last modified:February 1, 1995 - v1
Checksum:i44E3EFDDE1CDB35E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07163 Genomic DNA Translation: AAA20496.1
Z73565 Genomic DNA Translation: CAA97924.1
AY693182 Genomic DNA Translation: AAT93201.1
BK006949 Genomic DNA Translation: DAA11227.1
PIRiS47923
RefSeqiNP_015115.1, NM_001184023.1

Genome annotation databases

EnsemblFungiiYPL209C; YPL209C; YPL209C
GeneIDi855892
KEGGisce:YPL209C

Similar proteinsi

Entry informationi

Entry nameiIPL1_YEAST
AccessioniPrimary (citable) accession number: P38991
Secondary accession number(s): D6W3G1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 185 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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