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Protein

SNF1-activating kinase 1

Gene

SAK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that phosphorylates SNF1, the catalytic subunit of the SNF1 kinase complex. Acts as an activator of the SNF1 kinase complex and controls its nuclear localization upon glucose and nitrogen depletion. Also required for SNF1 kinase activation under other stress conditions like alkaline pH or presence of cadmium.13 Publications

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei162ATPPROSITE-ProRule annotation1
Active sitei277Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi139 – 147ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • DNA-dependent DNA replication Source: SGD
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: SGD
  • protein phosphorylation Source: SGD
  • pseudohyphal growth Source: SGD
  • regulation of glycogen biosynthetic process Source: SGD
  • regulation of protein localization to nucleus Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30292-MONOMER
BRENDAi2.7.11.1 984
ReactomeiR-SCE-380972 Energy dependent regulation of mTOR by LKB1-AMPK
R-SCE-442717 CREB phosphorylation through the activation of CaMKK

Names & Taxonomyi

Protein namesi
Recommended name:
SNF1-activating kinase 1 (EC:2.7.11.1)
Gene namesi
Name:SAK1
Synonyms:PAK1
Ordered Locus Names:YER129W
ORF Names:SYGP-ORF45
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER129W
SGDiS000000931 SAK1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864641 – 1142SNF1-activating kinase 1Add BLAST1142

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43PhosphothreonineCombined sources1
Modified residuei964PhosphoserineCombined sources1
Modified residuei1126PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38990
PaxDbiP38990
PRIDEiP38990

PTM databases

iPTMnetiP38990

Interactioni

Subunit structurei

Associates with the SNF1 kinase complex. Interacts with SNF1 and REG1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006842EBI-12863,EBI-4192

Protein-protein interaction databases

BioGridi36873, 158 interactors
DIPiDIP-5407N
IntActiP38990, 14 interactors
MINTiP38990
STRINGi4932.YER129W

Structurei

3D structure databases

ProteinModelPortaliP38990
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 448Protein kinasePROSITE-ProRule annotationAdd BLAST316

Domaini

The kinase domain is not sufficient by themself for proper function and that the non-conserved N-terminal and C-terminal domains are critical for the biological activity. The C-terminus promotes interaction of ELM1 and TOS3 kinases with SNF1.2 Publications

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063214
HOGENOMiHOG000115426
InParanoidiP38990
KOiK21157
OMAiAIMKKCH
OrthoDBiEOG092C4F3T

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P38990-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRSDKKVNV EEVNVPSNLQ IELEKSGTSS SVSLRSPTKS SATNLAGMAE
60 70 80 90 100
GARDNASIAS SSVDSLNMLL ERQRVRQLNH PQHQQHISSS LAKTPTTTSS
110 120 130 140 150
FCSSGSSKNK VKETNRISLT YDPVSKRKVL NTYEIIKELG HGQHGKVKLA
160 170 180 190 200
RDILSKQLVA IKIVDRHEKK QRKFFTFIKS SKISENDKIK REIAIMKKCH
210 220 230 240 250
HKHVVQLIEV LDDLKSRKIY LVLEYCSRGE VKWCPPDCME SDAKGPSLLS
260 270 280 290 300
FQETREILRG VVLGLEYLHY QGIIHRDIKP ANLLISGDGT VKISDFGVSL
310 320 330 340 350
AASSTNSSDS SESLDELELA KTVGTPAFFA PEMCLGEDAF TRYNLTKENL
360 370 380 390 400
FRGSCISFMI DIWAVGVTLY CLLFGMLPFF SDFELKLFEK IVNDPLKFPT
410 420 430 440 450
FKEIQSNKVS KVSCEEEYEM AKDLLLKLLE KNPQKRMTIP AIKKHPFVSW
460 470 480 490 500
DFDHVPENDE KLLSSVLEQK LRFQCNQTDQ FEPISISKHE LKNAVSGVGK
510 520 530 540 550
KIKESVLKSI PLKDPSDLSN KNYLHPTETT RGRGDANVIV SEGSVLSNIK
560 570 580 590 600
ELSANDGCLN TDSDTNININ DDDHYSGDDN DGHLTKRELE RELNKFDDKH
610 620 630 640 650
EAGNMVNLPI NSSFASLDSF YIDNFAMARM GMSSPEAGDS VSSVPNLPSA
660 670 680 690 700
PSSTRLGRSP VFSGVTNQPS PIRPVLPQQK SSFCATGRYD KSHNSLLRNS
710 720 730 740 750
SSHLTSYNSG RPSSRTGRMN SRNQNLPKIP NSLSKISTTK LTELRVPKDS
760 770 780 790 800
EIPSPAKNPN ADRLRRFPVK KNTKTPAIKD PPRININSSD KSGSKNSPIK
810 820 830 840 850
SLYQRMKQSK DNSKTFEVRR GNFFSHFNGD DDDSSSQSSV TSSGSESDSE
860 870 880 890 900
LSSTSSSCTS GTQSRNSSNN NAYSETESLP FEFGVDSEDG SGVLLRDLPN
910 920 930 940 950
EDQIRPFLDI QPCRRMKVKS SLNLEPPSVS SSSSSSSDED ELILNVGTAG
960 970 980 990 1000
HRRRHNSSKL SELSNSPQKG SNNFMYSNGS VHDSETTITP QNMDDLTLHQ
1010 1020 1030 1040 1050
ALSRSQPISK PGPLVLPKRL DQKKATTETS NLTDIVEFNG NNDHRKDKNF
1060 1070 1080 1090 1100
DKVLYSRDLL KDALSSTNAG RRRSIPSNKI RGRKDASITM STNVGNDEHA
1110 1120 1130 1140
RNTSCHGDKG QENGAIKQRT HERSRSLTVA ELNEEKRRSA LP
Length:1,142
Mass (Da):126,872
Last modified:February 1, 1995 - v1
Checksum:i425D71B8340B3F8F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171Q → H in AAC49840 (PubMed:9341678).Curated1
Sequence conflicti266 – 268EYL → DS in AAC49840 (PubMed:9341678).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18916 Genomic DNA Translation: AAC03227.1
U13398 Genomic DNA Translation: AAC49840.1 Different termination.
BK006939 Genomic DNA Translation: DAA07789.1
PIRiS50632
RefSeqiNP_011055.3, NM_001179019.3

Genome annotation databases

EnsemblFungiiYER129W; YER129W; YER129W
GeneIDi856866
KEGGisce:YER129W

Similar proteinsi

Entry informationi

Entry nameiSAK1_YEAST
AccessioniPrimary (citable) accession number: P38990
Secondary accession number(s): D3DM35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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