P38989 (SMC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Structural maintenance of chromosomes protein 2

Alternative name(s):
DA-box protein SMC2

Gene names

Name:	SMC2
Ordered Locus Names:	YFR031C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1170 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Subunit structure	Forms a heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRN1, YCS4 and YCG1/YCS5. Ref.5
Subcellular location	Nucleus. Cytoplasm. Chromosome. Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, condensin associates with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
Domain	The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer By similarity.
Miscellaneous	Present with 3290 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SMC family. SMC2 subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA condensation Mitosis
Cellular component	Chromosome Cytoplasm Nucleus
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro cell division Inferred from electronic annotation. Source: UniProtKB-KW mitotic chromosome condensation Inferred from mutant phenotype Ref.5Ref.1. Source: SGD sister chromatid cohesion Inferred from electronic annotation. Source: InterPro tRNA gene clustering Inferred from mutant phenotype PubMed 18708579. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD nuclear condensin complex Inferred from physical interaction Ref.1. Source: SGD
Molecular_function	AT DNA binding Inferred from direct assay PubMed 9727028. Source: SGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from direct assay PubMed 9727028. Source: SGD DNA secondary structure binding Inferred from direct assay PubMed 9727028. Source: SGD double-stranded DNA binding Inferred from direct assay PubMed 9727028. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRN1	P38170	2	EBI-17412,EBI-4792
SMC1	P32908	3	EBI-17412,EBI-17402
YCG1	Q06680	3	EBI-17412,EBI-4799

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1170	1170	Structural maintenance of chromosomes protein 2		PRO_0000119013
Regions
	Nucleotide binding	32 – 39	8	ATP Potential
	Region	470 – 677	208	Flexible hinge
	Coiled coil	172 – 469	298	Potential
	Coiled coil	678 – 1027	350	Potential
	Compositional bias	1084 – 1119	36	Ala/Asp-rich (DA-box)
Amino acid modifications
	Modified residue	1085	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P38989 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 142B41AAE109621F	FASTA	1,170	133,928
	10 20 30 40 50 60 MKVEELIIDG FKSYATRTVI TDWDPQFNAI TGLNGSGKSN ILDAICFVLG IASMSTVRAS 70 80 90 100 110 120 SLQDLIYKRG QAGVTKASVT IVFDNTDKSN SPIGFTNSPQ ISVTRQVVLG GTSKYLINGH 130 140 150 160 170 180 RAPQQSVLQL FQSVQLNINN PNFLIMQGKI TKVLNMKPSE ILSLIEEAAG TKMFEDRREK 190 200 210 220 230 240 AERTMSKKET KLQENRTLLT EEIEPKLEKL RNEKRMFLEF QSTQTDLEKT ERIVVSYEYY 250 260 270 280 290 300 NIKHKHTSIR ETLENGETRM KMLNEFVKKT SEEIDSLNED VEEIKLQKEK ELHKEGTISK 310 320 330 340 350 360 LENKENGLLN EISRLKTSLS IKVENLNDTT EKSKALESEI ASSSAKLIEK KSAYANTEKD 370 380 390 400 410 420 YKMVQEQLSK QRDLYKRKEE LVSTLTTGIS STGAADGGYN AQLAKAKTEL NEVSLAIKKS 430 440 450 460 470 480 SMKMELLKKE LLTIEPKLKE ATKDNELNVK HVKQCQETCD KLRARLVEYG FDPSRIKDLK 490 500 510 520 530 540 QREDKLKSHY YQTCKNSEYL KRRVTNLEFN YTKPYPNFEA SFVHGVVGQL FQIDNDNIRY 550 560 570 580 590 600 ATALQTCAGG RLFNVVVQDS QTATQLLERG RLRKRVTIIP LDKIYTRPIS SQVLDLAKKI 610 620 630 640 650 660 APGKVELAIN LIRFDESITK AMEFIFGNSL ICEDPETAKK ITFHPKIRAR SITLQGDVYD 670 680 690 700 710 720 PEGTLSGGSR NTSESLLVDI QKYNQIQKQI ETIQADLNHV TEELQTQYAT SQKTKTIQSD 730 740 750 760 770 780 LNLSLHKLDL AKRNLDANPS SQIIARNEEI LRDIGECENE IKTKQMSLKK CQEEVSTIEK 790 800 810 820 830 840 DMKEYDSDKG SKLNELKKEL KLLAKELEEQ ESESERKYDL FQNLELETEQ LSSELDSNKT 850 860 870 880 890 900 LLHNHLKSIE SLKLENSDLE GKIRGVEDDL VTVQTELNEE KKRLMDIDDE LNELETLIKK 910 920 930 940 950 960 KQDEKKSSEL ELQKLVHDLN KYKSNTNNME KIIEDLRQKH EFLEDFDLVR NIVKQNEGID 970 980 990 1000 1010 1020 LDTYRERSKQ LNEKFQELRK KVNPNIMNMI ENVEKKEAAL KTMIKTIEKD KMKIQETISK 1030 1040 1050 1060 1070 1080 LNEYKRETLV KTWEKVTLDF GNIFADLLPN SFAKLVPCEG KDVTQGLEVK VKLGNIWKES 1090 1100 1110 1120 1130 1140 LIELSGGQRS LIALSLIMAL LQFRPAPMYI LDEVDAALDL SHTQNIGHLI KTRFKGSQFI 1150 1160 1170 VVSLKEGMFA NANRVFRTRF QDGTSVVSIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family." Strunnikov A.V., Hogan E., Koshland D. Genes Dev. 9:587-599(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y. Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"The condensin complex governs chromosome condensation and mitotic transmission of rDNA." Freeman L., Aragon-Alcaide L., Strunnikov A.V. J. Cell Biol. 149:811-824(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A CONDENSIN COMPLEX WITH SMC4; BRN1; YCS4 AND YCG1.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1085, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U05820 Genomic DNA. Translation: AAA17416.1. D50617 Genomic DNA. Translation: BAA09270.1. BK006940 Genomic DNA. Translation: DAA12471.1.
PIR	A56157.
RefSeq	NP_116687.1. NM_001179996.2.
3D structure databases
ProteinModelPortal	P38989.
SMR	P38989. Positions 3-170, 507-691, 1029-1169.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2983N.
IntAct	P38989. 12 interactions.
MINT	MINT-435495.
STRING	4932.YFR031C.
Proteomic databases
PaxDb	P38989.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR031C; YFR031C; YFR031C.
GeneID	850589.
KEGG	sce:YFR031C.
Organism-specific databases
CYGD	YFR031c.
SGD	S000001927. SMC2.
Phylogenomic databases
eggNOG	COG1196.
GeneTree	ENSGT00550000074857.
HOGENOM	HOG000163792.
KO	K06674.
OMA	SECESAE.
OrthoDB	EOG4P2T9B.
Gene expression databases
Genevestigator	P38989.
GermOnline	YFR031C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003395. RecF/RecN/SMC. IPR024704. SMC. IPR027120. Smc2. IPR010935. SMC_hinge. [Graphical view]
PANTHER	PTHR18937:SF9. PTHR18937:SF9. 1 hit.
Pfam	PF06470. SMC_hinge. 1 hit. PF02463. SMC_N. 1 hit. [Graphical view]
PIRSF	PIRSF005719. SMC. 1 hit.
SMART	SM00968. SMC_hinge. 1 hit. [Graphical view]
SUPFAM	SSF75553. SMC_hinge. 1 hit.
ProtoNet	Search...
Other
NextBio	966434.

Entry information

Entry name

SMC2_YEAST

Accession

Primary (citable) accession number: P38989
Secondary accession number(s): D6VTR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program