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P38989 (SMC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Structural maintenance of chromosomes protein 2
Alternative name(s):
DA-box protein SMC2
Gene names
Name:SMC2
Ordered Locus Names:YFR031C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1170 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

Subunit structure

Forms a heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRN1, YCS4 and YCG1/YCS5. Ref.5

Subcellular location

Nucleus. Cytoplasm. Chromosome. Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, condensin associates with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

Domain

The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer By similarity.

Miscellaneous

Present with 3290 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SMC family. SMC2 subfamily.

Ontologies

Keywords
   Biological processCell cycle
Cell division
DNA condensation
Mitosis
   Cellular componentChromosome
Cytoplasm
Nucleus
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from direct assay PubMed 12719426. Source: GOC

DNA recombination

Inferred from electronic annotation. Source: InterPro

DNA repair

Inferred from electronic annotation. Source: InterPro

meiotic chromosome condensation

Inferred by curator Ref.5. Source: SGD

meiotic chromosome separation

Inferred by curator Ref.5. Source: SGD

mitotic chromosome condensation

Inferred from mutant phenotype Ref.5. Source: SGD

mitotic sister chromatid segregation

Inferred from mutant phenotype Ref.5. Source: SGD

rDNA condensation

Inferred from mutant phenotype Ref.5. Source: SGD

synaptonemal complex assembly

Inferred by curator Ref.5. Source: SGD

tRNA gene clustering

Inferred from mutant phenotype PubMed 18708579. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nuclear condensin complex

Inferred from direct assay Ref.5. Source: SGD

   Molecular_functionAT DNA binding

Inferred from direct assay PubMed 9727028. Source: SGD

ATP binding

Inferred from direct assay PubMed 9727028. Source: SGD

DNA secondary structure binding

Inferred from direct assay PubMed 9727028. Source: SGD

chromatin binding

Inferred from direct assay PubMed 16055730. Source: SGD

double-stranded DNA binding

Inferred from direct assay PubMed 9727028. Source: SGD

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11701170Structural maintenance of chromosomes protein 2
PRO_0000119013

Regions

Nucleotide binding32 – 398ATP Potential
Region470 – 677208Flexible hinge
Coiled coil172 – 469298 Potential
Coiled coil678 – 1027350 Potential
Compositional bias1084 – 111936Ala/Asp-rich (DA-box)

Sequences

Sequence LengthMass (Da)Tools
P38989 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 142B41AAE109621F

FASTA1,170133,928
        10         20         30         40         50         60 
MKVEELIIDG FKSYATRTVI TDWDPQFNAI TGLNGSGKSN ILDAICFVLG IASMSTVRAS 

        70         80         90        100        110        120 
SLQDLIYKRG QAGVTKASVT IVFDNTDKSN SPIGFTNSPQ ISVTRQVVLG GTSKYLINGH 

       130        140        150        160        170        180 
RAPQQSVLQL FQSVQLNINN PNFLIMQGKI TKVLNMKPSE ILSLIEEAAG TKMFEDRREK 

       190        200        210        220        230        240 
AERTMSKKET KLQENRTLLT EEIEPKLEKL RNEKRMFLEF QSTQTDLEKT ERIVVSYEYY 

       250        260        270        280        290        300 
NIKHKHTSIR ETLENGETRM KMLNEFVKKT SEEIDSLNED VEEIKLQKEK ELHKEGTISK 

       310        320        330        340        350        360 
LENKENGLLN EISRLKTSLS IKVENLNDTT EKSKALESEI ASSSAKLIEK KSAYANTEKD 

       370        380        390        400        410        420 
YKMVQEQLSK QRDLYKRKEE LVSTLTTGIS STGAADGGYN AQLAKAKTEL NEVSLAIKKS 

       430        440        450        460        470        480 
SMKMELLKKE LLTIEPKLKE ATKDNELNVK HVKQCQETCD KLRARLVEYG FDPSRIKDLK 

       490        500        510        520        530        540 
QREDKLKSHY YQTCKNSEYL KRRVTNLEFN YTKPYPNFEA SFVHGVVGQL FQIDNDNIRY 

       550        560        570        580        590        600 
ATALQTCAGG RLFNVVVQDS QTATQLLERG RLRKRVTIIP LDKIYTRPIS SQVLDLAKKI 

       610        620        630        640        650        660 
APGKVELAIN LIRFDESITK AMEFIFGNSL ICEDPETAKK ITFHPKIRAR SITLQGDVYD 

       670        680        690        700        710        720 
PEGTLSGGSR NTSESLLVDI QKYNQIQKQI ETIQADLNHV TEELQTQYAT SQKTKTIQSD 

       730        740        750        760        770        780 
LNLSLHKLDL AKRNLDANPS SQIIARNEEI LRDIGECENE IKTKQMSLKK CQEEVSTIEK 

       790        800        810        820        830        840 
DMKEYDSDKG SKLNELKKEL KLLAKELEEQ ESESERKYDL FQNLELETEQ LSSELDSNKT 

       850        860        870        880        890        900 
LLHNHLKSIE SLKLENSDLE GKIRGVEDDL VTVQTELNEE KKRLMDIDDE LNELETLIKK 

       910        920        930        940        950        960 
KQDEKKSSEL ELQKLVHDLN KYKSNTNNME KIIEDLRQKH EFLEDFDLVR NIVKQNEGID 

       970        980        990       1000       1010       1020 
LDTYRERSKQ LNEKFQELRK KVNPNIMNMI ENVEKKEAAL KTMIKTIEKD KMKIQETISK 

      1030       1040       1050       1060       1070       1080 
LNEYKRETLV KTWEKVTLDF GNIFADLLPN SFAKLVPCEG KDVTQGLEVK VKLGNIWKES 

      1090       1100       1110       1120       1130       1140 
LIELSGGQRS LIALSLIMAL LQFRPAPMYI LDEVDAALDL SHTQNIGHLI KTRFKGSQFI 

      1150       1160       1170 
VVSLKEGMFA NANRVFRTRF QDGTSVVSIM 

« Hide

References

« Hide 'large scale' references
[1]"SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family."
Strunnikov A.V., Hogan E., Koshland D.
Genes Dev. 9:587-599(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[5]"The condensin complex governs chromosome condensation and mitotic transmission of rDNA."
Freeman L., Aragon-Alcaide L., Strunnikov A.V.
J. Cell Biol. 149:811-824(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A CONDENSIN COMPLEX WITH SMC4; BRN1; YCS4 AND YCG1.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U05820 Genomic DNA. Translation: AAA17416.1.
D50617 Genomic DNA. Translation: BAA09270.1.
BK006940 Genomic DNA. Translation: DAA12471.1.
PIRA56157.
RefSeqNP_116687.1. NM_001179996.2.

3D structure databases

ProteinModelPortalP38989.
SMRP38989. Positions 1-221, 526-661, 950-1169.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31186. 54 interactions.
DIPDIP-2983N.
IntActP38989. 12 interactions.
MINTMINT-435495.
STRING4932.YFR031C.

Proteomic databases

PaxDbP38989.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFR031C; YFR031C; YFR031C.
GeneID850589.
KEGGsce:YFR031C.

Organism-specific databases

CYGDYFR031c.
SGDS000001927. SMC2.

Phylogenomic databases

eggNOGCOG1196.
GeneTreeENSGT00550000074857.
HOGENOMHOG000163792.
KOK06674.
OMACQNGKIP.
OrthoDBEOG7M0P0T.

Enzyme and pathway databases

BioCycYEAST:G3O-30479-MONOMER.

Gene expression databases

GenevestigatorP38989.

Family and domain databases

Gene3D3.40.50.300. 3 hits.
InterProIPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
IPR024704. SMC.
IPR027120. Smc2.
IPR010935. SMC_hinge.
[Graphical view]
PANTHERPTHR18937:SF9. PTHR18937:SF9. 1 hit.
PfamPF06470. SMC_hinge. 1 hit.
PF02463. SMC_N. 1 hit.
[Graphical view]
PIRSFPIRSF005719. SMC. 1 hit.
SMARTSM00968. SMC_hinge. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF75553. SSF75553. 1 hit.
ProtoNetSearch...

Other

NextBio966434.
PROP38989.

Entry information

Entry nameSMC2_YEAST
AccessionPrimary (citable) accession number: P38989
Secondary accession number(s): D6VTR1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families