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P38989

- SMC2_YEAST

UniProt

P38989 - SMC2_YEAST

Protein

Structural maintenance of chromosomes protein 2

Gene

SMC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi32 – 398ATPSequence Analysis

    GO - Molecular functioni

    1. AT DNA binding Source: SGD
    2. ATP binding Source: SGD
    3. chromatin binding Source: SGD
    4. DNA secondary structure binding Source: SGD
    5. double-stranded DNA binding Source: SGD
    6. protein binding Source: IntAct

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. meiotic chromosome condensation Source: SGD
    3. meiotic chromosome separation Source: SGD
    4. mitotic chromosome condensation Source: SGD
    5. mitotic sister chromatid segregation Source: SGD
    6. rDNA condensation Source: SGD
    7. synaptonemal complex assembly Source: SGD
    8. tRNA gene clustering Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, DNA condensation, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30479-MONOMER.
    ReactomeiREACT_188908. Condensation of Prometaphase Chromosomes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Structural maintenance of chromosomes protein 2
    Alternative name(s):
    DA-box protein SMC2
    Gene namesi
    Name:SMC2
    Ordered Locus Names:YFR031C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR031c.
    SGDiS000001927. SMC2.

    Subcellular locationi

    Nucleus. Cytoplasm. Chromosome
    Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, condensin associates with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nuclear condensin complex Source: SGD

    Keywords - Cellular componenti

    Chromosome, Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11701170Structural maintenance of chromosomes protein 2PRO_0000119013Add
    BLAST

    Proteomic databases

    MaxQBiP38989.
    PaxDbiP38989.

    Expressioni

    Gene expression databases

    GenevestigatoriP38989.

    Interactioni

    Subunit structurei

    Forms a heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRN1, YCS4 and YCG1/YCS5.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BRN1P381702EBI-17412,EBI-4792
    SMC1P329083EBI-17412,EBI-17402
    YCG1Q066803EBI-17412,EBI-4799

    Protein-protein interaction databases

    BioGridi31186. 54 interactions.
    DIPiDIP-2983N.
    IntActiP38989. 12 interactions.
    MINTiMINT-435495.
    STRINGi4932.YFR031C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38989.
    SMRiP38989. Positions 1-221, 507-691, 950-1169.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni470 – 677208Flexible hingeAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili172 – 469298Sequence AnalysisAdd
    BLAST
    Coiled coili678 – 1027350Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1084 – 111936Ala/Asp-rich (DA-box)Add
    BLAST

    Domaini

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.By similarity

    Sequence similaritiesi

    Belongs to the SMC family. SMC2 subfamily.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG1196.
    GeneTreeiENSGT00550000074857.
    HOGENOMiHOG000163792.
    KOiK06674.
    OMAiCQNGKIP.
    OrthoDBiEOG7M0P0T.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR027417. P-loop_NTPase.
    IPR003395. RecF/RecN/SMC_N.
    IPR024704. SMC.
    IPR027120. Smc2.
    IPR010935. SMC_hinge.
    [Graphical view]
    PANTHERiPTHR18937:SF9. PTHR18937:SF9. 1 hit.
    PfamiPF06470. SMC_hinge. 1 hit.
    PF02463. SMC_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005719. SMC. 1 hit.
    SMARTiSM00968. SMC_hinge. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF75553. SSF75553. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38989-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVEELIIDG FKSYATRTVI TDWDPQFNAI TGLNGSGKSN ILDAICFVLG     50
    IASMSTVRAS SLQDLIYKRG QAGVTKASVT IVFDNTDKSN SPIGFTNSPQ 100
    ISVTRQVVLG GTSKYLINGH RAPQQSVLQL FQSVQLNINN PNFLIMQGKI 150
    TKVLNMKPSE ILSLIEEAAG TKMFEDRREK AERTMSKKET KLQENRTLLT 200
    EEIEPKLEKL RNEKRMFLEF QSTQTDLEKT ERIVVSYEYY NIKHKHTSIR 250
    ETLENGETRM KMLNEFVKKT SEEIDSLNED VEEIKLQKEK ELHKEGTISK 300
    LENKENGLLN EISRLKTSLS IKVENLNDTT EKSKALESEI ASSSAKLIEK 350
    KSAYANTEKD YKMVQEQLSK QRDLYKRKEE LVSTLTTGIS STGAADGGYN 400
    AQLAKAKTEL NEVSLAIKKS SMKMELLKKE LLTIEPKLKE ATKDNELNVK 450
    HVKQCQETCD KLRARLVEYG FDPSRIKDLK QREDKLKSHY YQTCKNSEYL 500
    KRRVTNLEFN YTKPYPNFEA SFVHGVVGQL FQIDNDNIRY ATALQTCAGG 550
    RLFNVVVQDS QTATQLLERG RLRKRVTIIP LDKIYTRPIS SQVLDLAKKI 600
    APGKVELAIN LIRFDESITK AMEFIFGNSL ICEDPETAKK ITFHPKIRAR 650
    SITLQGDVYD PEGTLSGGSR NTSESLLVDI QKYNQIQKQI ETIQADLNHV 700
    TEELQTQYAT SQKTKTIQSD LNLSLHKLDL AKRNLDANPS SQIIARNEEI 750
    LRDIGECENE IKTKQMSLKK CQEEVSTIEK DMKEYDSDKG SKLNELKKEL 800
    KLLAKELEEQ ESESERKYDL FQNLELETEQ LSSELDSNKT LLHNHLKSIE 850
    SLKLENSDLE GKIRGVEDDL VTVQTELNEE KKRLMDIDDE LNELETLIKK 900
    KQDEKKSSEL ELQKLVHDLN KYKSNTNNME KIIEDLRQKH EFLEDFDLVR 950
    NIVKQNEGID LDTYRERSKQ LNEKFQELRK KVNPNIMNMI ENVEKKEAAL 1000
    KTMIKTIEKD KMKIQETISK LNEYKRETLV KTWEKVTLDF GNIFADLLPN 1050
    SFAKLVPCEG KDVTQGLEVK VKLGNIWKES LIELSGGQRS LIALSLIMAL 1100
    LQFRPAPMYI LDEVDAALDL SHTQNIGHLI KTRFKGSQFI VVSLKEGMFA 1150
    NANRVFRTRF QDGTSVVSIM 1170
    Length:1,170
    Mass (Da):133,928
    Last modified:February 1, 1995 - v1
    Checksum:i142B41AAE109621F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05820 Genomic DNA. Translation: AAA17416.1.
    D50617 Genomic DNA. Translation: BAA09270.1.
    BK006940 Genomic DNA. Translation: DAA12471.1.
    PIRiA56157.
    RefSeqiNP_116687.1. NM_001179996.2.

    Genome annotation databases

    EnsemblFungiiYFR031C; YFR031C; YFR031C.
    GeneIDi850589.
    KEGGisce:YFR031C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05820 Genomic DNA. Translation: AAA17416.1 .
    D50617 Genomic DNA. Translation: BAA09270.1 .
    BK006940 Genomic DNA. Translation: DAA12471.1 .
    PIRi A56157.
    RefSeqi NP_116687.1. NM_001179996.2.

    3D structure databases

    ProteinModelPortali P38989.
    SMRi P38989. Positions 1-221, 507-691, 950-1169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31186. 54 interactions.
    DIPi DIP-2983N.
    IntActi P38989. 12 interactions.
    MINTi MINT-435495.
    STRINGi 4932.YFR031C.

    Proteomic databases

    MaxQBi P38989.
    PaxDbi P38989.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR031C ; YFR031C ; YFR031C .
    GeneIDi 850589.
    KEGGi sce:YFR031C.

    Organism-specific databases

    CYGDi YFR031c.
    SGDi S000001927. SMC2.

    Phylogenomic databases

    eggNOGi COG1196.
    GeneTreei ENSGT00550000074857.
    HOGENOMi HOG000163792.
    KOi K06674.
    OMAi CQNGKIP.
    OrthoDBi EOG7M0P0T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30479-MONOMER.
    Reactomei REACT_188908. Condensation of Prometaphase Chromosomes.

    Miscellaneous databases

    NextBioi 966434.
    PROi P38989.

    Gene expression databases

    Genevestigatori P38989.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    InterProi IPR027417. P-loop_NTPase.
    IPR003395. RecF/RecN/SMC_N.
    IPR024704. SMC.
    IPR027120. Smc2.
    IPR010935. SMC_hinge.
    [Graphical view ]
    PANTHERi PTHR18937:SF9. PTHR18937:SF9. 1 hit.
    Pfami PF06470. SMC_hinge. 1 hit.
    PF02463. SMC_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005719. SMC. 1 hit.
    SMARTi SM00968. SMC_hinge. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF75553. SSF75553. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family."
      Strunnikov A.V., Hogan E., Koshland D.
      Genes Dev. 9:587-599(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
      Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
      Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    5. "The condensin complex governs chromosome condensation and mitotic transmission of rDNA."
      Freeman L., Aragon-Alcaide L., Strunnikov A.V.
      J. Cell Biol. 149:811-824(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A CONDENSIN COMPLEX WITH SMC4; BRN1; YCS4 AND YCG1.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSMC2_YEAST
    AccessioniPrimary (citable) accession number: P38989
    Secondary accession number(s): D6VTR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3290 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3