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Protein

Protein TEM1

Gene

TEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in termination of M phase. May play a role in triggering the degradation of G2 cyclin to inactivate M-phase promoting factor at the termination of mitosis. Acts upstream of CDC15 kinase and may be required to activate the CDC15 protein kinase pathway.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 348GTPBy similarity
Nucleotide bindingi75 – 795GTPBy similarity
Nucleotide bindingi132 – 1354GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • establishment of mitotic spindle localization Source: SGD
  • exit from mitosis Source: SGD
  • metabolic process Source: GOC
  • mitotic spindle orientation checkpoint Source: SGD
  • regulation of protein localization to mitotic spindle pole body Source: SGD
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32659-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TEM1
Gene namesi
Name:TEM1
Ordered Locus Names:YML064C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYML064c.
EuPathDBiFungiDB:YML064C.
SGDiS000004529. TEM1.

Subcellular locationi

GO - Cellular componenti

  • spindle pole body Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Protein TEM1PRO_0000122466Add
BLAST

Proteomic databases

MaxQBiP38987.
PaxDbiP38987.
PeptideAtlasiP38987.

Interactioni

Subunit structurei

Interacts with CDC15 and AMN1. AMN1 and CDC15 compete for association with TEM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AMN1P382856EBI-19113,EBI-20853
BUB2P264482EBI-19113,EBI-3824
CDC15P276362EBI-19113,EBI-4200

Protein-protein interaction databases

BioGridi35102. 126 interactions.
DIPiDIP-1691N.
IntActiP38987. 50 interactions.
MINTiMINT-383751.
STRINGi4932.YML064C.

Structurei

3D structure databases

ProteinModelPortaliP38987.
SMRiP38987. Positions 25-172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 244229Small GTPase-likeAdd
BLAST

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00790000123010.
HOGENOMiHOG000216335.
InParanoidiP38987.
KOiK06682.
OMAiRLDLRWA.
OrthoDBiEOG73RBPK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38987-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT
60 70 80 90 100
QTLGVNFLKR KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF
110 120 130 140 150
DLTRPETLSS IKEWYRQAYG LNDSAIPILV GTKYDLLIDL DPEYQEQISR
160 170 180 190 200
TSMKYAQVMN APLIFCSTAK SINIQKIFKI ALAKIFNLTL TIPEINEIGD
210 220 230 240
PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS PGVNT
Length:245
Mass (Da):27,296
Last modified:February 1, 1995 - v1
Checksum:iF650C8946A03707C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38172 Genomic DNA. Translation: BAA07371.1.
Z38114 Genomic DNA. Translation: CAA86257.1.
AY557980 Genomic DNA. Translation: AAS56306.1.
BK006946 Genomic DNA. Translation: DAA09833.1.
PIRiS48334.
RefSeqiNP_013647.1. NM_001182423.1.

Genome annotation databases

EnsemblFungiiYML064C; YML064C; YML064C.
GeneIDi854938.
KEGGisce:YML064C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38172 Genomic DNA. Translation: BAA07371.1.
Z38114 Genomic DNA. Translation: CAA86257.1.
AY557980 Genomic DNA. Translation: AAS56306.1.
BK006946 Genomic DNA. Translation: DAA09833.1.
PIRiS48334.
RefSeqiNP_013647.1. NM_001182423.1.

3D structure databases

ProteinModelPortaliP38987.
SMRiP38987. Positions 25-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35102. 126 interactions.
DIPiDIP-1691N.
IntActiP38987. 50 interactions.
MINTiMINT-383751.
STRINGi4932.YML064C.

Proteomic databases

MaxQBiP38987.
PaxDbiP38987.
PeptideAtlasiP38987.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML064C; YML064C; YML064C.
GeneIDi854938.
KEGGisce:YML064C.

Organism-specific databases

CYGDiYML064c.
EuPathDBiFungiDB:YML064C.
SGDiS000004529. TEM1.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00790000123010.
HOGENOMiHOG000216335.
InParanoidiP38987.
KOiK06682.
OMAiRLDLRWA.
OrthoDBiEOG73RBPK.

Enzyme and pathway databases

BioCyciYEAST:G3O-32659-MONOMER.

Miscellaneous databases

NextBioi977983.
PROiP38987.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase."
    Shirayama M., Matsui Y., Toh-e A.
    Mol. Cell. Biol. 14:7476-7482(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling."
    Wang Y., Shirogane T., Liu D., Harper J.W., Elledge S.J.
    Cell 112:697-709(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH AMN1 AND CDC15.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTEM1_YEAST
AccessioniPrimary (citable) accession number: P38987
Secondary accession number(s): D6VZA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 24, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.