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Protein

Protein TEM1

Gene

TEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in termination of M phase. May play a role in triggering the degradation of G2 cyclin to inactivate M-phase promoting factor at the termination of mitosis. Acts upstream of CDC15 kinase and may be required to activate the CDC15 protein kinase pathway.1 Publication

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34GTPBy similarity8
Nucleotide bindingi75 – 79GTPBy similarity5
Nucleotide bindingi132 – 135GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • establishment of mitotic spindle localization Source: SGD
  • exit from mitosis Source: SGD
  • mitotic spindle orientation checkpoint Source: SGD
  • regulation of protein localization to mitotic spindle pole body Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32659-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TEM1
Gene namesi
Name:TEM1
Ordered Locus Names:YML064C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML064C.
SGDiS000004529. TEM1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224661 – 245Protein TEM1Add BLAST245

Proteomic databases

MaxQBiP38987.
PRIDEiP38987.

PTM databases

iPTMnetiP38987.

Interactioni

Subunit structurei

Interacts with CDC15 and AMN1. AMN1 and CDC15 compete for association with TEM1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35102. 345 interactors.
DIPiDIP-1691N.
IntActiP38987. 121 interactors.
MINTiMINT-383751.
STRINGi4932.YML064C.

Structurei

3D structure databases

ProteinModelPortaliP38987.
SMRiP38987.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 244Small GTPase-likeAdd BLAST229

Phylogenomic databases

GeneTreeiENSGT00800000124173.
HOGENOMiHOG000216335.
InParanoidiP38987.
KOiK06682.
OMAiLKCVIPE.
OrthoDBiEOG092C4PMQ.

Family and domain databases

CDDicd04128. Spg1. 1 hit.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017231. Small_GTPase_Tem1/Spg1.
PANTHERiPTHR24073:SF875. PTHR24073:SF875. 1 hit.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
PIRSFiPIRSF037527. Small_GTPase_Tem1. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51419. RAB. 1 hit.

Sequencei

Sequence statusi: Complete.

P38987-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT
60 70 80 90 100
QTLGVNFLKR KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF
110 120 130 140 150
DLTRPETLSS IKEWYRQAYG LNDSAIPILV GTKYDLLIDL DPEYQEQISR
160 170 180 190 200
TSMKYAQVMN APLIFCSTAK SINIQKIFKI ALAKIFNLTL TIPEINEIGD
210 220 230 240
PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS PGVNT
Length:245
Mass (Da):27,296
Last modified:February 1, 1995 - v1
Checksum:iF650C8946A03707C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38172 Genomic DNA. Translation: BAA07371.1.
Z38114 Genomic DNA. Translation: CAA86257.1.
AY557980 Genomic DNA. Translation: AAS56306.1.
BK006946 Genomic DNA. Translation: DAA09833.1.
PIRiS48334.
RefSeqiNP_013647.1. NM_001182423.1.

Genome annotation databases

EnsemblFungiiYML064C; YML064C; YML064C.
GeneIDi854938.
KEGGisce:YML064C.

Similar proteinsi

Entry informationi

Entry nameiTEM1_YEAST
AccessioniPrimary (citable) accession number: P38987
Secondary accession number(s): D6VZA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 22, 2017
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names