P38987 (TEM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein TEM1

Gene names

Name:	TEM1
Ordered Locus Names:	YML064C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTP-binding protein involved in termination of M phase. May play a role in triggering the degradation of G2 cyclin to inactivate M-phase promoting factor at the termination of mitosis. Acts upstream of CDC15 kinase and may be required to activate the CDC15 protein kinase pathway. Ref.5
Subunit structure	Interacts with CDC15 and AMN1. AMN1 and CDC15 compete for association with TEM1. Ref.5
Miscellaneous	Present with 573 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of exit from mitosis Traceable author statement PubMed 16039591. Source: SGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	spindle pole body Inferred from direct assay PubMed 19154724. Source: SGD
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay PubMed 12048186. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AMN1	P38285	6	EBI-19113,EBI-20853
BFA1	P47113	2	EBI-19113,EBI-3586
BUB2	P26448	2	EBI-19113,EBI-3824
CDC15	P27636	6	EBI-19113,EBI-4200

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Protein TEM1		PRO_0000122466
Regions
	Nucleotide binding	27 – 34	8	GTP By similarity
	Nucleotide binding	75 – 79	5	GTP By similarity
	Nucleotide binding	132 – 135	4	GTP By similarity
	Region	16 – 244	229	Small GTPase-like

Sequences

	Sequence		Length	Mass (Da)
	P38987 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: F650C8946A03707C	FASTA	245	27,296
	10 20 30 40 50 60 MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT QTLGVNFLKR 70 80 90 100 110 120 KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF DLTRPETLSS IKEWYRQAYG 130 140 150 160 170 180 LNDSAIPILV GTKYDLLIDL DPEYQEQISR TSMKYAQVMN APLIFCSTAK SINIQKIFKI 190 200 210 220 230 240 ALAKIFNLTL TIPEINEIGD PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS PGVNT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase." Shirayama M., Matsui Y., Toh-e A. Mol. Cell. Biol. 14:7476-7482(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling." Wang Y., Shirogane T., Liu D., Harper J.W., Elledge S.J. Cell 112:697-709(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH AMN1 AND CDC15.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38172 Genomic DNA. Translation: BAA07371.1. Z38114 Genomic DNA. Translation: CAA86257.1. AY557980 Genomic DNA. Translation: AAS56306.1. BK006946 Genomic DNA. Translation: DAA09833.1.
PIR	S48334.
RefSeq	NP_013647.1. NM_001182423.1.
3D structure databases
ProteinModelPortal	P38987.
SMR	P38987. Positions 19-202.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1691N.
IntAct	P38987. 87 interactions.
MINT	MINT-383751.
STRING	4932.YML064C.
Proteomic databases
PaxDb	P38987.
PeptideAtlas	P38987.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML064C; YML064C; YML064C.
GeneID	854938.
KEGG	sce:YML064C.
Organism-specific databases
CYGD	YML064c.
SGD	S000004529. TEM1.
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000216335.
KO	K06682.
OMA	DSQIGKT.
OrthoDB	EOG4C5GTN.
Gene expression databases
Genevestigator	P38987.
GermOnline	YML064C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR017231. Small_GTPase_Tem1. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PIRSF	PIRSF037527. Small_GTPase_Tem1. 1 hit.
PRINTS	PR00449. RASTRNSFRMNG.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977983.

Entry information

Entry name

TEM1_YEAST

Accession

Primary (citable) accession number: P38987
Secondary accession number(s): D6VZA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program