Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38987 (TEM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein TEM1
Gene names
Name:TEM1
Ordered Locus Names:YML064C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTP-binding protein involved in termination of M phase. May play a role in triggering the degradation of G2 cyclin to inactivate M-phase promoting factor at the termination of mitosis. Acts upstream of CDC15 kinase and may be required to activate the CDC15 protein kinase pathway. Ref.5

Subunit structure

Interacts with CDC15 and AMN1. AMN1 and CDC15 compete for association with TEM1. Ref.5

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245Protein TEM1
PRO_0000122466

Regions

Nucleotide binding27 – 348GTP By similarity
Nucleotide binding75 – 795GTP By similarity
Nucleotide binding132 – 1354GTP By similarity
Region16 – 244229Small GTPase-like

Sequences

Sequence LengthMass (Da)Tools
P38987 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: F650C8946A03707C

FASTA24527,296
        10         20         30         40         50         60 
MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT QTLGVNFLKR 

        70         80         90        100        110        120 
KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF DLTRPETLSS IKEWYRQAYG 

       130        140        150        160        170        180 
LNDSAIPILV GTKYDLLIDL DPEYQEQISR TSMKYAQVMN APLIFCSTAK SINIQKIFKI 

       190        200        210        220        230        240 
ALAKIFNLTL TIPEINEIGD PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS 


PGVNT 

« Hide

References

« Hide 'large scale' references
[1]"The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase."
Shirayama M., Matsui Y., Toh-e A.
Mol. Cell. Biol. 14:7476-7482(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling."
Wang Y., Shirogane T., Liu D., Harper J.W., Elledge S.J.
Cell 112:697-709(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH AMN1 AND CDC15.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D38172 Genomic DNA. Translation: BAA07371.1.
Z38114 Genomic DNA. Translation: CAA86257.1.
AY557980 Genomic DNA. Translation: AAS56306.1.
BK006946 Genomic DNA. Translation: DAA09833.1.
PIRS48334.
RefSeqNP_013647.1. NM_001182423.1.

3D structure databases

ProteinModelPortalP38987.
SMRP38987. Positions 25-172.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35102. 124 interactions.
DIPDIP-1691N.
IntActP38987. 50 interactions.
MINTMINT-383751.
STRING4932.YML064C.

Proteomic databases

PaxDbP38987.
PeptideAtlasP38987.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML064C; YML064C; YML064C.
GeneID854938.
KEGGsce:YML064C.

Organism-specific databases

CYGDYML064c.
SGDS000004529. TEM1.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00750000117736.
HOGENOMHOG000216335.
KOK06682.
OMAKTISVRR.
OrthoDBEOG73RBPK.

Enzyme and pathway databases

BioCycYEAST:G3O-32659-MONOMER.

Gene expression databases

GenevestigatorP38987.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017231. Small_GTPase_Tem1.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PIRSFPIRSF037527. Small_GTPase_Tem1. 1 hit.
PRINTSPR00449. RASTRNSFRMNG.
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977983.

Entry information

Entry nameTEM1_YEAST
AccessionPrimary (citable) accession number: P38987
Secondary accession number(s): D6VZA9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD