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P38987

- TEM1_YEAST

UniProt

P38987 - TEM1_YEAST

Protein

Protein TEM1

Gene

TEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    GTP-binding protein involved in termination of M phase. May play a role in triggering the degradation of G2 cyclin to inactivate M-phase promoting factor at the termination of mitosis. Acts upstream of CDC15 kinase and may be required to activate the CDC15 protein kinase pathway.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi27 – 348GTPBy similarity
    Nucleotide bindingi75 – 795GTPBy similarity
    Nucleotide bindingi132 – 1354GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. GTP catabolic process Source: GOC
    2. mitotic nuclear division Source: UniProtKB-KW
    3. regulation of exit from mitosis Source: SGD
    4. small GTPase mediated signal transduction Source: InterPro

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32659-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein TEM1
    Gene namesi
    Name:TEM1
    Ordered Locus Names:YML064C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML064c.
    SGDiS000004529. TEM1.

    Subcellular locationi

    GO - Cellular componenti

    1. spindle pole body Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 245245Protein TEM1PRO_0000122466Add
    BLAST

    Proteomic databases

    MaxQBiP38987.
    PaxDbiP38987.
    PeptideAtlasiP38987.

    Expressioni

    Gene expression databases

    GenevestigatoriP38987.

    Interactioni

    Subunit structurei

    Interacts with CDC15 and AMN1. AMN1 and CDC15 compete for association with TEM1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AMN1P382856EBI-19113,EBI-20853
    BUB2P264482EBI-19113,EBI-3824
    CDC15P276362EBI-19113,EBI-4200

    Protein-protein interaction databases

    BioGridi35102. 124 interactions.
    DIPiDIP-1691N.
    IntActiP38987. 50 interactions.
    MINTiMINT-383751.
    STRINGi4932.YML064C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38987.
    SMRiP38987. Positions 25-172.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni16 – 244229Small GTPase-likeAdd
    BLAST

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00750000117736.
    HOGENOMiHOG000216335.
    KOiK06682.
    OMAiEPILIYV.
    OrthoDBiEOG73RBPK.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR017231. Small_GTPase_Tem1.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037527. Small_GTPase_Tem1. 1 hit.
    PRINTSiPR00449. RASTRNSFRMNG.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38987-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT    50
    QTLGVNFLKR KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF 100
    DLTRPETLSS IKEWYRQAYG LNDSAIPILV GTKYDLLIDL DPEYQEQISR 150
    TSMKYAQVMN APLIFCSTAK SINIQKIFKI ALAKIFNLTL TIPEINEIGD 200
    PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS PGVNT 245
    Length:245
    Mass (Da):27,296
    Last modified:February 1, 1995 - v1
    Checksum:iF650C8946A03707C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38172 Genomic DNA. Translation: BAA07371.1.
    Z38114 Genomic DNA. Translation: CAA86257.1.
    AY557980 Genomic DNA. Translation: AAS56306.1.
    BK006946 Genomic DNA. Translation: DAA09833.1.
    PIRiS48334.
    RefSeqiNP_013647.1. NM_001182423.1.

    Genome annotation databases

    EnsemblFungiiYML064C; YML064C; YML064C.
    GeneIDi854938.
    KEGGisce:YML064C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38172 Genomic DNA. Translation: BAA07371.1 .
    Z38114 Genomic DNA. Translation: CAA86257.1 .
    AY557980 Genomic DNA. Translation: AAS56306.1 .
    BK006946 Genomic DNA. Translation: DAA09833.1 .
    PIRi S48334.
    RefSeqi NP_013647.1. NM_001182423.1.

    3D structure databases

    ProteinModelPortali P38987.
    SMRi P38987. Positions 25-172.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35102. 124 interactions.
    DIPi DIP-1691N.
    IntActi P38987. 50 interactions.
    MINTi MINT-383751.
    STRINGi 4932.YML064C.

    Proteomic databases

    MaxQBi P38987.
    PaxDbi P38987.
    PeptideAtlasi P38987.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML064C ; YML064C ; YML064C .
    GeneIDi 854938.
    KEGGi sce:YML064C.

    Organism-specific databases

    CYGDi YML064c.
    SGDi S000004529. TEM1.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00750000117736.
    HOGENOMi HOG000216335.
    KOi K06682.
    OMAi EPILIYV.
    OrthoDBi EOG73RBPK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32659-MONOMER.

    Miscellaneous databases

    NextBioi 977983.

    Gene expression databases

    Genevestigatori P38987.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR017231. Small_GTPase_Tem1.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037527. Small_GTPase_Tem1. 1 hit.
    PRINTSi PR00449. RASTRNSFRMNG.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase."
      Shirayama M., Matsui Y., Toh-e A.
      Mol. Cell. Biol. 14:7476-7482(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling."
      Wang Y., Shirogane T., Liu D., Harper J.W., Elledge S.J.
      Cell 112:697-709(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH AMN1 AND CDC15.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTEM1_YEAST
    AccessioniPrimary (citable) accession number: P38987
    Secondary accession number(s): D6VZA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 573 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3