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P38987

- TEM1_YEAST

UniProt

P38987 - TEM1_YEAST

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Protein
Protein TEM1
Gene
TEM1, YML064C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in termination of M phase. May play a role in triggering the degradation of G2 cyclin to inactivate M-phase promoting factor at the termination of mitosis. Acts upstream of CDC15 kinase and may be required to activate the CDC15 protein kinase pathway.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 348GTP By similarity
Nucleotide bindingi75 – 795GTP By similarity
Nucleotide bindingi132 – 1354GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTPase activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. mitotic nuclear division Source: UniProtKB-KW
  3. regulation of exit from mitosis Source: SGD
  4. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32659-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TEM1
Gene namesi
Name:TEM1
Ordered Locus Names:YML064C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML064c.
SGDiS000004529. TEM1.

Subcellular locationi

GO - Cellular componenti

  1. spindle pole body Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Protein TEM1
PRO_0000122466Add
BLAST

Proteomic databases

MaxQBiP38987.
PaxDbiP38987.
PeptideAtlasiP38987.

Expressioni

Gene expression databases

GenevestigatoriP38987.

Interactioni

Subunit structurei

Interacts with CDC15 and AMN1. AMN1 and CDC15 compete for association with TEM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AMN1P382856EBI-19113,EBI-20853
BUB2P264482EBI-19113,EBI-3824
CDC15P276362EBI-19113,EBI-4200

Protein-protein interaction databases

BioGridi35102. 124 interactions.
DIPiDIP-1691N.
IntActiP38987. 50 interactions.
MINTiMINT-383751.
STRINGi4932.YML064C.

Structurei

3D structure databases

ProteinModelPortaliP38987.
SMRiP38987. Positions 25-172.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 244229Small GTPase-like
Add
BLAST

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00750000117736.
HOGENOMiHOG000216335.
KOiK06682.
OMAiEPILIYV.
OrthoDBiEOG73RBPK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017231. Small_GTPase_Tem1.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PIRSFiPIRSF037527. Small_GTPase_Tem1. 1 hit.
PRINTSiPR00449. RASTRNSFRMNG.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38987-1 [UniParc]FASTAAdd to Basket

« Hide

MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT    50
QTLGVNFLKR KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF 100
DLTRPETLSS IKEWYRQAYG LNDSAIPILV GTKYDLLIDL DPEYQEQISR 150
TSMKYAQVMN APLIFCSTAK SINIQKIFKI ALAKIFNLTL TIPEINEIGD 200
PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS PGVNT 245
Length:245
Mass (Da):27,296
Last modified:February 1, 1995 - v1
Checksum:iF650C8946A03707C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38172 Genomic DNA. Translation: BAA07371.1.
Z38114 Genomic DNA. Translation: CAA86257.1.
AY557980 Genomic DNA. Translation: AAS56306.1.
BK006946 Genomic DNA. Translation: DAA09833.1.
PIRiS48334.
RefSeqiNP_013647.1. NM_001182423.1.

Genome annotation databases

EnsemblFungiiYML064C; YML064C; YML064C.
GeneIDi854938.
KEGGisce:YML064C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38172 Genomic DNA. Translation: BAA07371.1 .
Z38114 Genomic DNA. Translation: CAA86257.1 .
AY557980 Genomic DNA. Translation: AAS56306.1 .
BK006946 Genomic DNA. Translation: DAA09833.1 .
PIRi S48334.
RefSeqi NP_013647.1. NM_001182423.1.

3D structure databases

ProteinModelPortali P38987.
SMRi P38987. Positions 25-172.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35102. 124 interactions.
DIPi DIP-1691N.
IntActi P38987. 50 interactions.
MINTi MINT-383751.
STRINGi 4932.YML064C.

Proteomic databases

MaxQBi P38987.
PaxDbi P38987.
PeptideAtlasi P38987.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML064C ; YML064C ; YML064C .
GeneIDi 854938.
KEGGi sce:YML064C.

Organism-specific databases

CYGDi YML064c.
SGDi S000004529. TEM1.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00750000117736.
HOGENOMi HOG000216335.
KOi K06682.
OMAi EPILIYV.
OrthoDBi EOG73RBPK.

Enzyme and pathway databases

BioCyci YEAST:G3O-32659-MONOMER.

Miscellaneous databases

NextBioi 977983.

Gene expression databases

Genevestigatori P38987.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017231. Small_GTPase_Tem1.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PIRSFi PIRSF037527. Small_GTPase_Tem1. 1 hit.
PRINTSi PR00449. RASTRNSFRMNG.
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase."
    Shirayama M., Matsui Y., Toh-e A.
    Mol. Cell. Biol. 14:7476-7482(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling."
    Wang Y., Shirogane T., Liu D., Harper J.W., Elledge S.J.
    Cell 112:697-709(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH AMN1 AND CDC15.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTEM1_YEAST
AccessioniPrimary (citable) accession number: P38987
Secondary accession number(s): D6VZA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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