Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-asparaginase 1

Gene

ASP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Yeast contains two L-asparaginase isoenzymes: cytoplasmic L-asparaginase I, and cell wall L-asparaginase II.

Catalytic activityi

L-asparagine + H2O = L-aspartate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei64O-isoaspartyl threonine intermediatePROSITE-ProRule annotation1
Binding sitei110SubstrateBy similarity1

GO - Molecular functioni

  • asparaginase activity Source: SGD

GO - Biological processi

  • asparagine catabolic process Source: SGD

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciYEAST:YDR321W-MONOMER.
BRENDAi3.5.1.1. 984.
SABIO-RKiP38986.

Names & Taxonomyi

Protein namesi
Recommended name:
L-asparaginase 1 (EC:3.5.1.1)
Alternative name(s):
L-asparaginase I
L-asparagine amidohydrolase I
Short name:
ASP I
Gene namesi
Name:ASP1
Ordered Locus Names:YDR321W
ORF Names:D9798.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR321W.
SGDiS000002729. ASP1.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176A → V: Almost no activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001710911 – 381L-asparaginase 1Add BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei350PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38986.
PRIDEiP38986.

PTM databases

iPTMnetiP38986.

Expressioni

Inductioni

Expressed constitutively.

Interactioni

Subunit structurei

Homopolymer.

Protein-protein interaction databases

BioGridi32378. 36 interactors.
DIPiDIP-4030N.
IntActiP38986. 18 interactors.
MINTiMINT-497546.
STRINGi4932.YDR321W.

Structurei

3D structure databases

ProteinModelPortaliP38986.
SMRiP38986.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 378Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST325

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 142Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the asparaginase 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00510000050435.
HOGENOMiHOG000044165.
InParanoidiP38986.
KOiK01424.
OMAiKRSMDGM.
OrthoDBiEOG092C3Z6Q.

Family and domain databases

Gene3Di3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
InterProiView protein in InterPro
IPR004550. AsnASE_II.
IPR036152. Asp/glu_Ase-like_sf.
IPR006034. Asparaginase/glutaminase-like.
IPR020827. Asparaginase/glutaminase_AS1.
IPR027475. Asparaginase/glutaminase_AS2.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
IPR037152. L-asparaginase_N_sf.
PfamiView protein in Pfam
PF00710. Asparaginase. 1 hit.
PIRSFiPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR00139. ASNGLNASE.
SMARTiView protein in SMART
SM00870. Asparaginase. 1 hit.
SUPFAMiSSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00520. asnASE_II. 1 hit.
PROSITEiView protein in PROSITE
PS00144. ASN_GLN_ASE_1. 1 hit.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P38986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSDSVEITT ICPDVENSQF VVQSNCPETI PEILKSQNAA VNGSGIACQQ
60 70 80 90 100
RSLPRIKILG TGGTIASKAI DSSQTAGYHV DLTIQDLLDA IPDISKVCDI
110 120 130 140 150
EYEQLCNVDS KDINEDILYK IYKGVSESLQ AFDGIVITHG TDTLSETAFF
160 170 180 190 200
IESTIDAGDV PIVFVGSMRP STSVSADGPM NLYQAICIAS NPKSRGRGVL
210 220 230 240 250
VSLNDQISSG YYITKTNANS LDSFNVRQGY LGNFVNNEIH YYYPPVKPQG
260 270 280 290 300
CHKFKLRVDG KHFKLPEVCI LYAHQAFPPA IVNLVADKYD GIVLATMGAG
310 320 330 340 350
SLPEEVNETC MKLSLPIVYS KRSMDGMVPI ANVPKKGSKE DNLIASGYLS
360 370 380
PEKSRILLQL CLAGNYTLEE IKHVFTGVYG G
Length:381
Mass (Da):41,395
Last modified:February 1, 1995 - v1
Checksum:i8204CB7C58B5B75F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z27406 Genomic DNA. Translation: CAA81794.1.
U32517 Genomic DNA. Translation: AAB64757.1.
BK006938 Genomic DNA. Translation: DAA12163.1.
PIRiS48513.
RefSeqiNP_010607.3. NM_001180629.3.

Genome annotation databases

EnsemblFungiiYDR321W; YDR321W; YDR321W.
GeneIDi851920.
KEGGisce:YDR321W.

Similar proteinsi

Entry informationi

Entry nameiASPG1_YEAST
AccessioniPrimary (citable) accession number: P38986
Secondary accession number(s): D6VSV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 22, 2017
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names