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Protein

L-asparaginase 1

Gene

ASP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Yeast contains two L-asparaginase isoenzymes: cytoplasmic L-asparaginase I, and cell wall L-asparaginase II.

Catalytic activityi

L-asparagine + H2O = L-aspartate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei64O-isoaspartyl threonine intermediatePROSITE-ProRule annotation1
Binding sitei110SubstrateBy similarity1

GO - Molecular functioni

  • asparaginase activity Source: SGD

GO - Biological processi

  • asparagine catabolic process Source: SGD

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciYEAST:YDR321W-MONOMER
BRENDAi3.5.1.1 984
SABIO-RKiP38986

Names & Taxonomyi

Protein namesi
Recommended name:
L-asparaginase 1 (EC:3.5.1.1)
Alternative name(s):
L-asparaginase I
L-asparagine amidohydrolase I
Short name:
ASP I
Gene namesi
Name:ASP1
Ordered Locus Names:YDR321W
ORF Names:D9798.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR321W
SGDiS000002729 ASP1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176A → V: Almost no activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001710911 – 381L-asparaginase 1Add BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei350PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38986
PaxDbiP38986
PRIDEiP38986

PTM databases

iPTMnetiP38986

Expressioni

Inductioni

Expressed constitutively.

Interactioni

Subunit structurei

Homopolymer.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi3237837 interactors.
DIPiDIP-4030N
IntActiP38986 21 interactors.
MINTiP38986
STRINGi4932.YDR321W

Structurei

3D structure databases

ProteinModelPortaliP38986
SMRiP38986
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 378Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST325

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 142Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the asparaginase 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00510000050435
HOGENOMiHOG000044165
InParanoidiP38986
KOiK01424
OMAiARYITKT
OrthoDBiEOG092C3Z6Q

Family and domain databases

Gene3Di3.40.50.11701 hit
3.40.50.401 hit
InterProiView protein in InterPro
IPR004550 AsnASE_II
IPR036152 Asp/glu_Ase-like_sf
IPR006034 Asparaginase/glutaminase-like
IPR020827 Asparaginase/glutaminase_AS1
IPR027475 Asparaginase/glutaminase_AS2
IPR027473 L-asparaginase_C
IPR027474 L-asparaginase_N
IPR037152 L-asparaginase_N_sf
PfamiView protein in Pfam
PF00710 Asparaginase, 1 hit
PIRSFiPIRSF001220 L-ASNase_gatD, 1 hit
PRINTSiPR00139 ASNGLNASE
SMARTiView protein in SMART
SM00870 Asparaginase, 1 hit
SUPFAMiSSF53774 SSF53774, 1 hit
TIGRFAMsiTIGR00520 asnASE_II, 1 hit
PROSITEiView protein in PROSITE
PS00144 ASN_GLN_ASE_1, 1 hit
PS00917 ASN_GLN_ASE_2, 1 hit
PS51732 ASN_GLN_ASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

P38986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSDSVEITT ICPDVENSQF VVQSNCPETI PEILKSQNAA VNGSGIACQQ
60 70 80 90 100
RSLPRIKILG TGGTIASKAI DSSQTAGYHV DLTIQDLLDA IPDISKVCDI
110 120 130 140 150
EYEQLCNVDS KDINEDILYK IYKGVSESLQ AFDGIVITHG TDTLSETAFF
160 170 180 190 200
IESTIDAGDV PIVFVGSMRP STSVSADGPM NLYQAICIAS NPKSRGRGVL
210 220 230 240 250
VSLNDQISSG YYITKTNANS LDSFNVRQGY LGNFVNNEIH YYYPPVKPQG
260 270 280 290 300
CHKFKLRVDG KHFKLPEVCI LYAHQAFPPA IVNLVADKYD GIVLATMGAG
310 320 330 340 350
SLPEEVNETC MKLSLPIVYS KRSMDGMVPI ANVPKKGSKE DNLIASGYLS
360 370 380
PEKSRILLQL CLAGNYTLEE IKHVFTGVYG G
Length:381
Mass (Da):41,395
Last modified:February 1, 1995 - v1
Checksum:i8204CB7C58B5B75F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z27406 Genomic DNA Translation: CAA81794.1
U32517 Genomic DNA Translation: AAB64757.1
BK006938 Genomic DNA Translation: DAA12163.1
PIRiS48513
RefSeqiNP_010607.3, NM_001180629.3

Genome annotation databases

EnsemblFungiiYDR321W; YDR321W; YDR321W
GeneIDi851920
KEGGisce:YDR321W

Similar proteinsi

Entry informationi

Entry nameiASPG1_YEAST
AccessioniPrimary (citable) accession number: P38986
Secondary accession number(s): D6VSV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome