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Protein

Signal recognition particle subunit SRP14

Gene

SRP14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP14 binds scR1 RNA to form the probable Alu domain of SRP responsible for elongation arrest.

GO - Molecular functioni

  • 7S RNA binding Source: SGD
  • endoplasmic reticulum signal peptide binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29499-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle subunit SRP14
Alternative name(s):
Signal recognition particle 14 kDa protein homolog
Gene namesi
Name:SRP14
Ordered Locus Names:YDL092W
ORF Names:D2404
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL092W.
SGDiS000002250. SRP14.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • signal recognition particle, endoplasmic reticulum targeting Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001351961 – 146Signal recognition particle subunit SRP14Add BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38985.
PRIDEiP38985.

PTM databases

iPTMnetiP38985.

Interactioni

Subunit structurei

Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14. At least SRP14, SRP21, SRP68 and SRP72 are proposed to get assembled together with scR1 RNA as a pre-SRP complex in the nucleolus which is exported to the cytoplasm. SRP14 binds RNA as a homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SEC65P294787EBI-17977,EBI-16641
SRP72P386886EBI-17977,EBI-18011

Protein-protein interaction databases

BioGridi31968. 29 interactors.
DIPiDIP-4864N.
IntActiP38985. 17 interactors.
MINTiMINT-483620.

Structurei

3D structure databases

ProteinModelPortaliP38985.
SMRiP38985.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SRP14 family.Curated

Phylogenomic databases

HOGENOMiHOG000065969.
InParanoidiP38985.
KOiK03104.
OMAiNEKHITV.
OrthoDBiEOG092C5UDY.

Family and domain databases

Gene3Di3.30.720.10. 1 hit.
InterProiIPR003210. Signal_recog_particle_SRP14.
IPR009018. Signal_recog_particle_SRP9/14.
[Graphical view]
PfamiPF02290. SRP14. 1 hit.
[Graphical view]
SUPFAMiSSF54762. SSF54762. 1 hit.

Sequencei

Sequence statusi: Complete.

P38985-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANTGCLSPG AFLSKVPEFF QTANEKHITV RLTAKRLIEH DPVEGNLEFD
60 70 80 90 100
STNHPDYDVS KKASEISVSS RSDREYPLLI RMSYGSHDKK TKCSTVVKAS
110 120 130 140
ELDQFWQEYS SVFKGGMQNL IKKKKKKSKN GTISKTGKKN KVAKKN
Length:146
Mass (Da):16,430
Last modified:February 1, 1995 - v1
Checksum:i4AF76A26018367EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35155 Genomic DNA. Translation: AAA53402.1.
X95644 Genomic DNA. Translation: CAA64919.1.
Z74140 Genomic DNA. Translation: CAA98659.1.
Z74139 Genomic DNA. Translation: CAA98658.1.
AY558159 Genomic DNA. Translation: AAS56485.1.
BK006938 Genomic DNA. Translation: DAA11766.1.
PIRiS51616.
RefSeqiNP_010191.1. NM_001180151.1.

Genome annotation databases

EnsemblFungiiYDL092W; YDL092W; YDL092W.
GeneIDi851466.
KEGGisce:YDL092W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35155 Genomic DNA. Translation: AAA53402.1.
X95644 Genomic DNA. Translation: CAA64919.1.
Z74140 Genomic DNA. Translation: CAA98659.1.
Z74139 Genomic DNA. Translation: CAA98658.1.
AY558159 Genomic DNA. Translation: AAS56485.1.
BK006938 Genomic DNA. Translation: DAA11766.1.
PIRiS51616.
RefSeqiNP_010191.1. NM_001180151.1.

3D structure databases

ProteinModelPortaliP38985.
SMRiP38985.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31968. 29 interactors.
DIPiDIP-4864N.
IntActiP38985. 17 interactors.
MINTiMINT-483620.

PTM databases

iPTMnetiP38985.

Proteomic databases

MaxQBiP38985.
PRIDEiP38985.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL092W; YDL092W; YDL092W.
GeneIDi851466.
KEGGisce:YDL092W.

Organism-specific databases

EuPathDBiFungiDB:YDL092W.
SGDiS000002250. SRP14.

Phylogenomic databases

HOGENOMiHOG000065969.
InParanoidiP38985.
KOiK03104.
OMAiNEKHITV.
OrthoDBiEOG092C5UDY.

Enzyme and pathway databases

BioCyciYEAST:G3O-29499-MONOMER.

Miscellaneous databases

PROiP38985.

Family and domain databases

Gene3Di3.30.720.10. 1 hit.
InterProiIPR003210. Signal_recog_particle_SRP14.
IPR009018. Signal_recog_particle_SRP9/14.
[Graphical view]
PfamiPF02290. SRP14. 1 hit.
[Graphical view]
SUPFAMiSSF54762. SSF54762. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSRP14_YEAST
AccessioniPrimary (citable) accession number: P38985
Secondary accession number(s): D6VRQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.