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Protein

Basic amino-acid permease

Gene

ALP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity permease for basic amino acids.1 Publication

GO - Molecular functioni

GO - Biological processi

  • basic amino acid transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33264-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.11. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Basic amino-acid permease
Gene namesi
Name:ALP1
Ordered Locus Names:YNL270C
ORF Names:N0660
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL270C.
SGDiS000005214. ALP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7373CytoplasmicSequence analysisAdd
BLAST
Transmembranei74 – 9320HelicalSequence analysisAdd
BLAST
Topological domaini94 – 952ExtracellularSequence analysis
Transmembranei96 – 11621HelicalSequence analysisAdd
BLAST
Topological domaini117 – 15337CytoplasmicSequence analysisAdd
BLAST
Transmembranei154 – 17421HelicalSequence analysisAdd
BLAST
Topological domaini175 – 1795ExtracellularSequence analysis
Transmembranei180 – 20021HelicalSequence analysisAdd
BLAST
Topological domaini201 – 2099CytoplasmicSequence analysis
Transmembranei210 – 23021HelicalSequence analysisAdd
BLAST
Topological domaini231 – 26535ExtracellularSequence analysisAdd
BLAST
Transmembranei266 – 28621HelicalSequence analysisAdd
BLAST
Topological domaini287 – 30620CytoplasmicSequence analysisAdd
BLAST
Transmembranei307 – 32721HelicalSequence analysisAdd
BLAST
Topological domaini328 – 35932ExtracellularSequence analysisAdd
BLAST
Transmembranei360 – 38021HelicalSequence analysisAdd
BLAST
Topological domaini381 – 40323CytoplasmicSequence analysisAdd
BLAST
Transmembranei404 – 42421HelicalSequence analysisAdd
BLAST
Topological domaini425 – 4317ExtracellularSequence analysis
Transmembranei432 – 45221HelicalSequence analysisAdd
BLAST
Topological domaini453 – 47725CytoplasmicSequence analysisAdd
BLAST
Transmembranei478 – 49821HelicalSequence analysisAdd
BLAST
Topological domaini499 – 51012ExtracellularSequence analysisAdd
BLAST
Transmembranei511 – 53121HelicalSequence analysisAdd
BLAST
Topological domaini532 – 57342CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
  • vacuole Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 573573Basic amino-acid permeasePRO_0000054145Add
BLAST

Proteomic databases

TopDownProteomicsiP38971.

Interactioni

Protein-protein interaction databases

BioGridi35570. 38 interactions.
IntActiP38971. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP38971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261850.
InParanoidiP38971.
KOiK16261.
OMAiGEQTSFR.
OrthoDBiEOG74J9HZ.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDETVNIQMS KEGQYEINSS SIIKEEEFVD EQYSGENVTK AITTERKVED
60 70 80 90 100
DAAKETESSP QERREVKRKL KQRHIGMIAL GGTIGTGLII GIGPPLAHAG
110 120 130 140 150
PVGALISYLF MGTVIYSVTQ SLGEMVTFIP VTSSFSVFAQ RFLSPALGAT
160 170 180 190 200
NGYMYWLSWC FTFALELSVL GKVIQYWTEA VPLAAWIVIF WCLLTSMNMF
210 220 230 240 250
PVKYYGEFEF CIASIKVIAL LGFIIFSFCV VCGAGQSDGP IGFRYWRNPG
260 270 280 290 300
AWGPGIISSD KNEGRFLGWV SSLINAAFTY QGTELVGITA GEAANPRKAL
310 320 330 340 350
PRAIKKVVVR ILVFYILSLF FIGLLVPYND PKLDSDGIFV SSSPFMISIE
360 370 380 390 400
NSGTKVLPDI FNAVVLITIL SAGNSNVYIG SRVLYSLSKN SLAPRFLSNV
410 420 430 440 450
TRGGVPYFSV LSTSVFGFLA FLEVSAGSGK AFNWLLNITG VAGFFAWLLI
460 470 480 490 500
SFSHIRFMQA IRKRGISRDD LPYKAQMMPF LAYYASFFIA LIVLIQGFTA
510 520 530 540 550
FAPTFQPIDF VAAYISIFLF LAIWLSFQVW FKCRLLWKLQ DIDIDSDRRQ
560 570
IEELVWIEPE CKTRWQRVWD VLS
Length:573
Mass (Da):64,014
Last modified:October 1, 1996 - v2
Checksum:i359DFE1466C348A1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511D → DD in CAA52199 (PubMed:7941748).Curated
Sequence conflicti126 – 1261V → A in CAA52199 (PubMed:7941748).Curated
Sequence conflicti260 – 2601D → N in CAA52199 (PubMed:7941748).Curated
Sequence conflicti517 – 5171I → V in CAA52199 (PubMed:7941748).Curated
Sequence conflicti548 – 5481R → H in CAA52199 (PubMed:7941748).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74069 Genomic DNA. Translation: CAA52199.1.
X92494 Genomic DNA. Translation: CAA63228.1.
Z71546 Genomic DNA. Translation: CAA96177.1.
BK006947 Genomic DNA. Translation: DAA10290.1.
PIRiS60912.
RefSeqiNP_014129.1. NM_001183108.1.

Genome annotation databases

EnsemblFungiiYNL270C; YNL270C; YNL270C.
GeneIDi855451.
KEGGisce:YNL270C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74069 Genomic DNA. Translation: CAA52199.1.
X92494 Genomic DNA. Translation: CAA63228.1.
Z71546 Genomic DNA. Translation: CAA96177.1.
BK006947 Genomic DNA. Translation: DAA10290.1.
PIRiS60912.
RefSeqiNP_014129.1. NM_001183108.1.

3D structure databases

ProteinModelPortaliP38971.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35570. 38 interactions.
IntActiP38971. 1 interaction.

Protein family/group databases

TCDBi2.A.3.10.11. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

TopDownProteomicsiP38971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL270C; YNL270C; YNL270C.
GeneIDi855451.
KEGGisce:YNL270C.

Organism-specific databases

EuPathDBiFungiDB:YNL270C.
SGDiS000005214. ALP1.

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261850.
InParanoidiP38971.
KOiK16261.
OMAiGEQTSFR.
OrthoDBiEOG74J9HZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-33264-MONOMER.

Miscellaneous databases

PROiP38971.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "APL1, a yeast gene encoding a putative permease for basic amino acids."
    Sychrova H., Chevallier M.R.
    Yeast 10:653-657(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes."
    Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H.
    Yeast 12:505-514(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae."
    Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S.
    Curr. Genet. 36:317-328(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiALP1_YEAST
AccessioniPrimary (citable) accession number: P38971
Secondary accession number(s): D6W0S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.