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P38971 (ALP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Basic amino-acid permease
Gene names
Name:ALP1
Ordered Locus Names:YNL270C
ORF Names:N0660
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length573 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-affinity permease for basic amino acids. Ref.5

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family. [View classification]

Ontologies

Keywords
   Biological processAmino-acid transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionbasic amino acid transmembrane transporter activity

Inferred from direct assay Ref.5. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 573573Basic amino-acid permease
PRO_0000054145

Regions

Topological domain1 – 7373Cytoplasmic Potential
Transmembrane74 – 9320Helical; Potential
Topological domain94 – 952Extracellular Potential
Transmembrane96 – 11621Helical; Potential
Topological domain117 – 15337Cytoplasmic Potential
Transmembrane154 – 17421Helical; Potential
Topological domain175 – 1795Extracellular Potential
Transmembrane180 – 20021Helical; Potential
Topological domain201 – 2099Cytoplasmic Potential
Transmembrane210 – 23021Helical; Potential
Topological domain231 – 26535Extracellular Potential
Transmembrane266 – 28621Helical; Potential
Topological domain287 – 30620Cytoplasmic Potential
Transmembrane307 – 32721Helical; Potential
Topological domain328 – 35932Extracellular Potential
Transmembrane360 – 38021Helical; Potential
Topological domain381 – 40323Cytoplasmic Potential
Transmembrane404 – 42421Helical; Potential
Topological domain425 – 4317Extracellular Potential
Transmembrane432 – 45221Helical; Potential
Topological domain453 – 47725Cytoplasmic Potential
Transmembrane478 – 49821Helical; Potential
Topological domain499 – 51012Extracellular Potential
Transmembrane511 – 53121Helical; Potential
Topological domain532 – 57342Cytoplasmic Potential

Experimental info

Sequence conflict511D → DD in CAA52199. Ref.1
Sequence conflict1261V → A in CAA52199. Ref.1
Sequence conflict2601D → N in CAA52199. Ref.1
Sequence conflict5171I → V in CAA52199. Ref.1
Sequence conflict5481R → H in CAA52199. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P38971 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 359DFE1466C348A1

FASTA57364,014
        10         20         30         40         50         60 
MDETVNIQMS KEGQYEINSS SIIKEEEFVD EQYSGENVTK AITTERKVED DAAKETESSP 

        70         80         90        100        110        120 
QERREVKRKL KQRHIGMIAL GGTIGTGLII GIGPPLAHAG PVGALISYLF MGTVIYSVTQ 

       130        140        150        160        170        180 
SLGEMVTFIP VTSSFSVFAQ RFLSPALGAT NGYMYWLSWC FTFALELSVL GKVIQYWTEA 

       190        200        210        220        230        240 
VPLAAWIVIF WCLLTSMNMF PVKYYGEFEF CIASIKVIAL LGFIIFSFCV VCGAGQSDGP 

       250        260        270        280        290        300 
IGFRYWRNPG AWGPGIISSD KNEGRFLGWV SSLINAAFTY QGTELVGITA GEAANPRKAL 

       310        320        330        340        350        360 
PRAIKKVVVR ILVFYILSLF FIGLLVPYND PKLDSDGIFV SSSPFMISIE NSGTKVLPDI 

       370        380        390        400        410        420 
FNAVVLITIL SAGNSNVYIG SRVLYSLSKN SLAPRFLSNV TRGGVPYFSV LSTSVFGFLA 

       430        440        450        460        470        480 
FLEVSAGSGK AFNWLLNITG VAGFFAWLLI SFSHIRFMQA IRKRGISRDD LPYKAQMMPF 

       490        500        510        520        530        540 
LAYYASFFIA LIVLIQGFTA FAPTFQPIDF VAAYISIFLF LAIWLSFQVW FKCRLLWKLQ 

       550        560        570 
DIDIDSDRRQ IEELVWIEPE CKTRWQRVWD VLS

« Hide

References

« Hide 'large scale' references
[1]"APL1, a yeast gene encoding a putative permease for basic amino acids."
Sychrova H., Chevallier M.R.
Yeast 10:653-657(1994) [PubMed: 7941748] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes."
Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H.
Yeast 12:505-514(1996) [PubMed: 8740425] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed: 9169873] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae."
Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S.
Curr. Genet. 36:317-328(1999) [PubMed: 10654085] [Abstract]
Cited for: FUNCTION.
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X74069 Genomic DNA. Translation: CAA52199.1.
X92494 Genomic DNA. Translation: CAA63228.1.
Z71546 Genomic DNA. Translation: CAA96177.1.
BK006947 Genomic DNA. Translation: DAA10290.1.
PIRS60912.
RefSeqNP_014129.1. NM_001183108.1.

3D structure databases

ProteinModelPortalP38971.
ModBaseSearch...

Protein-protein interaction databases

IntActP38971. 1 interaction.
STRINGP38971.

Protein family/group databases

TCDB2.A.3.10.11. amino acid-polyamine-organocation (APC) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL270C; YNL270C; YNL270C.
GeneID855451.
KEGGsce:YNL270C.
NMPDRfig|4932.3.peg.5194.

Organism-specific databases

CYGDYNL270c.
SGDS000005214. ALP1.

Phylogenomic databases

eggNOGfuNOG04851.
GeneTreeEFGT00050000001131.
HOGENOMHBG492579.
OMASAGECEN.
OrthoDBEOG4JDMG2.

Gene expression databases

ArrayExpressP38971.
GenevestigatorP38971.
GermOnlineYNL270C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004841. AA-permease_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERPTHR11785. AA/rel_permease1. 1 hit.
PfamPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsTIGR00913. 2A0310. 1 hit.
PROSITEPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979361.

Entry information

Entry nameALP1_YEAST
AccessionPrimary (citable) accession number: P38971
Secondary accession number(s): D6W0S4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: December 14, 2011
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents