Serine/threonine-protein kinase HAL5 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38970 (HAL5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase HAL5
EC=2.7.11.1

Alternative name(s):
Halotolerance protein 5

Gene names

Name:	HAL5
Ordered Locus Names:	YJL165C
ORF Names:	J0531

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	855 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein kinase involved in salt tolerance and pH sensitivity, probably by regulating plasma membrane potential and cation influx. Positively controls the TRK1-TRK2 potassium transport system in response to potassium starvation. Stabilizes TRK1 in the plasma membrane by preventing its vacuolar sorting and degradation. Also stabilizes other plasma membrane nutrient transporters like CAN1, FUR4 and HXT1. May itself be subject to regulation by ARL1. Ref.4 Ref.5 Ref.7
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NPR/HAL subfamily. HAL5 sub-subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAA50395.1 differs from that shown. Reason: Frameshift at positions 587 and 606.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular cation homeostasis Inferred from genetic interaction Ref.4. Source: SGD cellular protein localization Inferred from genetic interaction PubMed 21237705. Source: SGD protein dephosphorylation Inferred from mutant phenotype PubMed 21237705. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase activity Inferred from direct assay PubMed 16319894. Source: SGD protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 855

855

Serine/threonine-protein kinase HAL5

PRO_0000085988

Regions

Domain

503 – 837

335

Protein kinase

Nucleotide binding

509 – 517

ATP By similarity

Compositional bias

369 – 377

Poly-Gln

Sites

Active site

688

Proton acceptor By similarity

Binding site

546

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphothreonine Ref.8

Modified residue

Phosphoserine Ref.8 Ref.9

Modified residue

Phosphoserine Ref.8 Ref.9

Modified residue

Phosphoserine Ref.10

Modified residue

Phosphoserine Ref.6 Ref.10

Modified residue

Phosphoserine Ref.6 Ref.10

Modified residue

160

Phosphoserine Ref.10

Modified residue

217

Phosphoserine Ref.9

Modified residue

233

Phosphoserine Ref.9 Ref.10

Modified residue

273

Phosphoserine Ref.9 Ref.10

Modified residue

280

Phosphoserine Ref.10

Modified residue

324

Phosphoserine Ref.10

Modified residue

336

Phosphoserine Ref.10

Modified residue

358

Phosphoserine Ref.6

Modified residue

391

Phosphoserine Ref.6 Ref.10

Modified residue

395

Phosphoserine Ref.6 Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

P38970 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 4F23A97996489F39
Show »

FASTA

855

95,455

        10         20         30         40         50         60 
MGDEKLSRHT SLKRARSLSE SIKGLFKPSG ISGSNNAAAP SSRPGQDQAH SHQTARIITS 

        70         80         90        100        110        120 
NVSSPSISPV HSPVLQAAPK HHKLGVPNIA KLSLSPSREP SLNSENEMFS QESFISEKDE 

       130        140        150        160        170        180 
DEANLLERED LQNKKEEKAR AKHVRSKEAY VPHHRYTVGS DEVERQPRER LKNFPQNAGS 

       190        200        210        220        230        240 
SNPANSNANH VLDQENNFSI DAMLDYDEES KLRRRNSLGV RNHSNRTRSR KNSLSTPRSP 

       250        260        270        280        290        300 
PMKNGNGGMN SNATNNVGNG TGNRIYMRGR NHSDSISASS LPKFQEIECK CILDLGHFKV 

       310        320        330        340        350        360 
FENGYHEHSL RVLPIITNNK NVDSGDEKDA DASVNSGDDG DNDSEANMHK QKSVFSLSGL 

       370        380        390        400        410        420 
FKSHKDGNQQ QQQQQQQEEN GEQINLEKAF SIIPSQRFIK SQTLKKSRTS NLKNGNNDEL 

       430        440        450        460        470        480 
MKNDGKNIPQ IVNPNAAVGV EELKLINALS EKIRKGLKSE NTKGNNGEGR SNSNKQEDSD 

       490        500        510        520        530        540 
DTEGKAGTTN DDTSHKPCSQ KYGKSIGVVG AGAYGVVKIC ARCKTAKDVL PYSTYSNGKK 

       550        560        570        580        590        600 
LFFAVKELKP KPGDQIDKFC TRLTSEFIIG HSLSHPHFEA NAMIAGNVSR TTPPKHVFNA 

       610        620        630        640        650        660 
PNILKILDLM EYSNSFVEVM EFCASGDLYS LLTRNNISNE SNNGSSRLIQ TVKEGSGSPL 

       670        680        690        700        710        720 
HPLEADCFMK QLLNGVQYMH DHGIAHCDLK PENILFQPNG LLKICDFGTS SVFQTAWEKH 

       730        740        750        760        770        780 
VHFQSGAMGS EPYVAPEEFI RDAEYDPRLV DCWSCGIVYC TMVMGQYLWK IAIPEKDSLF 

       790        800        810        820        830        840 
KSFLSEIKDD GQFYLFEELR HVSSELNRLR KIALYRTFQV DPTKRITIEQ LLQSSWMRKT 

       850 
KCCVVYRHLH TKVSK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Saccharomyces cerevisiae HAL5 gene, mRNA." Kron S.J. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A novel mechanism of ion homeostasis and salt tolerance in yeast: the Hal4 and Hal5 protein kinases modulate the Trk1-Trk2 potassium transporter." Mulet J.M., Leube M.P., Kron S.J., Rios G., Fink G.R., Serrano R. Mol. Cell. Biol. 19:3328-3337(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Yeast ARL1 encodes a regulator of K+ influx." Munson A.M., Haydon D.H., Love S.L., Fell G.L., Palanivel V.R., Rosenwald A.G. J. Cell Sci. 117:2309-2320(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-72; SER-358; SER-391 AND SER-395, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Key role for intracellular K+ and protein kinases Sat4/Hal4 and Hal5 in the plasma membrane stabilization of yeast nutrient transporters." Perez-Valle J., Jenkins H., Merchan S., Montiel V., Ramos J., Sharma S., Serrano R., Yenush L. Mol. Cell. Biol. 27:5725-5736(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7; THR-10; SER-17 AND SER-19, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-19; SER-217; SER-233 AND SER-273, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63; SER-68; SER-72; SER-160; SER-233; SER-273; SER-280; SER-324; SER-336; SER-391 AND SER-395, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15300 mRNA. Translation: AAA50395.1. Frameshift. Z49440 Genomic DNA. Translation: CAA89460.1. BK006943 Genomic DNA. Translation: DAA08638.1.
PIR	S56948.
RefSeq	NP_012370.1. NM_001181598.1.
3D structure databases
ProteinModelPortal	P38970.
SMR	P38970. Positions 506-839.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6399N.
IntAct	P38970. 3 interactions.
MINT	MINT-689966.
STRING	4932.YJL165C.
Proteomic databases
PaxDb	P38970.
PeptideAtlas	P38970.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL165C; YJL165C; YJL165C.
GeneID	853274.
KEGG	sce:YJL165C.
Organism-specific databases
CYGD	YJL165c.
SGD	S000003701. HAL5.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00550000075309.
HOGENOM	HOG000112846.
KO	K08286.
OMA	IANADEK.
OrthoDB	EOG4J6W0P.
Gene expression databases
Genevestigator	P38970.
GermOnline	YJL165C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973553.

Entry information

Entry name

HAL5_YEAST

Accession

Primary (citable) accession number: P38970
Secondary accession number(s): D6VW22

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents