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P38970

- HAL5_YEAST

UniProt

P38970 - HAL5_YEAST

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Protein

Serine/threonine-protein kinase HAL5

Gene

HAL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Protein kinase involved in salt tolerance and pH sensitivity, probably by regulating plasma membrane potential and cation influx. Positively controls the TRK1-TRK2 potassium transport system in response to potassium starvation. Stabilizes TRK1 in the plasma membrane by preventing its vacuolar sorting and degradation. Also stabilizes other plasma membrane nutrient transporters like CAN1, FUR4 and HXT1. May itself be subject to regulation by ARL1.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei546 – 5461ATPPROSITE-ProRule annotation
Active sitei688 – 6881Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi509 – 5179ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein kinase activity Source: SGD
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. cellular cation homeostasis Source: SGD
  2. cellular protein localization Source: SGD
  3. protein dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31605-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase HAL5 (EC:2.7.11.1)
Alternative name(s):
Halotolerance protein 5
Gene namesi
Name:HAL5
Ordered Locus Names:YJL165C
ORF Names:J0531
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL165c.
SGDiS000003701. HAL5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 855855Serine/threonine-protein kinase HAL5PRO_0000085988Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171Phosphoserine1 Publication
Modified residuei19 – 191Phosphoserine1 Publication
Modified residuei68 – 681Phosphoserine3 Publications
Modified residuei72 – 721Phosphoserine2 Publications
Modified residuei160 – 1601Phosphoserine1 Publication
Modified residuei273 – 2731Phosphoserine1 Publication
Modified residuei277 – 2771Phosphoserine1 Publication
Modified residuei324 – 3241Phosphoserine2 Publications
Modified residuei333 – 3331Phosphoserine1 Publication
Modified residuei336 – 3361Phosphoserine2 Publications
Modified residuei358 – 3581Phosphoserine1 Publication
Modified residuei391 – 3911Phosphoserine1 Publication
Modified residuei395 – 3951Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38970.
PaxDbiP38970.
PeptideAtlasiP38970.

Expressioni

Gene expression databases

GenevestigatoriP38970.

Interactioni

Protein-protein interaction databases

BioGridi33594. 153 interactions.
DIPiDIP-6399N.
IntActiP38970. 3 interactions.
MINTiMINT-689966.
STRINGi4932.YJL165C.

Structurei

3D structure databases

ProteinModelPortaliP38970.
SMRiP38970. Positions 506-839.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini503 – 837335Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi369 – 3779Poly-Gln

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000075309.
HOGENOMiHOG000112846.
InParanoidiP38970.
KOiK08286.
OMAiIANADEK.
OrthoDBiEOG7008J9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38970-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGDEKLSRHT SLKRARSLSE SIKGLFKPSG ISGSNNAAAP SSRPGQDQAH
60 70 80 90 100
SHQTARIITS NVSSPSISPV HSPVLQAAPK HHKLGVPNIA KLSLSPSREP
110 120 130 140 150
SLNSENEMFS QESFISEKDE DEANLLERED LQNKKEEKAR AKHVRSKEAY
160 170 180 190 200
VPHHRYTVGS DEVERQPRER LKNFPQNAGS SNPANSNANH VLDQENNFSI
210 220 230 240 250
DAMLDYDEES KLRRRNSLGV RNHSNRTRSR KNSLSTPRSP PMKNGNGGMN
260 270 280 290 300
SNATNNVGNG TGNRIYMRGR NHSDSISASS LPKFQEIECK CILDLGHFKV
310 320 330 340 350
FENGYHEHSL RVLPIITNNK NVDSGDEKDA DASVNSGDDG DNDSEANMHK
360 370 380 390 400
QKSVFSLSGL FKSHKDGNQQ QQQQQQQEEN GEQINLEKAF SIIPSQRFIK
410 420 430 440 450
SQTLKKSRTS NLKNGNNDEL MKNDGKNIPQ IVNPNAAVGV EELKLINALS
460 470 480 490 500
EKIRKGLKSE NTKGNNGEGR SNSNKQEDSD DTEGKAGTTN DDTSHKPCSQ
510 520 530 540 550
KYGKSIGVVG AGAYGVVKIC ARCKTAKDVL PYSTYSNGKK LFFAVKELKP
560 570 580 590 600
KPGDQIDKFC TRLTSEFIIG HSLSHPHFEA NAMIAGNVSR TTPPKHVFNA
610 620 630 640 650
PNILKILDLM EYSNSFVEVM EFCASGDLYS LLTRNNISNE SNNGSSRLIQ
660 670 680 690 700
TVKEGSGSPL HPLEADCFMK QLLNGVQYMH DHGIAHCDLK PENILFQPNG
710 720 730 740 750
LLKICDFGTS SVFQTAWEKH VHFQSGAMGS EPYVAPEEFI RDAEYDPRLV
760 770 780 790 800
DCWSCGIVYC TMVMGQYLWK IAIPEKDSLF KSFLSEIKDD GQFYLFEELR
810 820 830 840 850
HVSSELNRLR KIALYRTFQV DPTKRITIEQ LLQSSWMRKT KCCVVYRHLH

TKVSK
Length:855
Mass (Da):95,455
Last modified:November 1, 1995 - v2
Checksum:i4F23A97996489F39
GO

Sequence cautioni

The sequence AAA50395.1 differs from that shown. Reason: Frameshift at positions 587 and 606. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15300 mRNA. Translation: AAA50395.1. Frameshift.
Z49440 Genomic DNA. Translation: CAA89460.1.
BK006943 Genomic DNA. Translation: DAA08638.1.
PIRiS56948.
RefSeqiNP_012370.1. NM_001181598.1.

Genome annotation databases

EnsemblFungiiYJL165C; YJL165C; YJL165C.
GeneIDi853274.
KEGGisce:YJL165C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15300 mRNA. Translation: AAA50395.1 . Frameshift.
Z49440 Genomic DNA. Translation: CAA89460.1 .
BK006943 Genomic DNA. Translation: DAA08638.1 .
PIRi S56948.
RefSeqi NP_012370.1. NM_001181598.1.

3D structure databases

ProteinModelPortali P38970.
SMRi P38970. Positions 506-839.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33594. 153 interactions.
DIPi DIP-6399N.
IntActi P38970. 3 interactions.
MINTi MINT-689966.
STRINGi 4932.YJL165C.

Proteomic databases

MaxQBi P38970.
PaxDbi P38970.
PeptideAtlasi P38970.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL165C ; YJL165C ; YJL165C .
GeneIDi 853274.
KEGGi sce:YJL165C.

Organism-specific databases

CYGDi YJL165c.
SGDi S000003701. HAL5.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000075309.
HOGENOMi HOG000112846.
InParanoidi P38970.
KOi K08286.
OMAi IANADEK.
OrthoDBi EOG7008J9.

Enzyme and pathway databases

BioCyci YEAST:G3O-31605-MONOMER.

Miscellaneous databases

NextBioi 973553.

Gene expression databases

Genevestigatori P38970.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Saccharomyces cerevisiae HAL5 gene, mRNA."
    Kron S.J.
    Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A novel mechanism of ion homeostasis and salt tolerance in yeast: the Hal4 and Hal5 protein kinases modulate the Trk1-Trk2 potassium transporter."
    Mulet J.M., Leube M.P., Kron S.J., Rios G., Fink G.R., Serrano R.
    Mol. Cell. Biol. 19:3328-3337(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: FUNCTION.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-72; SER-358; SER-391 AND SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Key role for intracellular K+ and protein kinases Sat4/Hal4 and Hal5 in the plasma membrane stabilization of yeast nutrient transporters."
    Perez-Valle J., Jenkins H., Merchan S., Montiel V., Ramos J., Sharma S., Serrano R., Yenush L.
    Mol. Cell. Biol. 27:5725-5736(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17 AND SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-160; SER-324; SER-336 AND SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-72; SER-273; SER-277; SER-324; SER-333; SER-336 AND SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHAL5_YEAST
AccessioniPrimary (citable) accession number: P38970
Secondary accession number(s): D6VW22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3