Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38970 (HAL5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase HAL5

EC=2.7.11.1
Alternative name(s):
Halotolerance protein 5
Gene names
Name:HAL5
Ordered Locus Names:YJL165C
ORF Names:J0531
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length855 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein kinase involved in salt tolerance and pH sensitivity, probably by regulating plasma membrane potential and cation influx. Positively controls the TRK1-TRK2 potassium transport system in response to potassium starvation. Stabilizes TRK1 in the plasma membrane by preventing its vacuolar sorting and degradation. Also stabilizes other plasma membrane nutrient transporters like CAN1, FUR4 and HXT1. May itself be subject to regulation by ARL1. Ref.4 Ref.5 Ref.7

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NPR/HAL subfamily. HAL5 sub-subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAA50395.1 differs from that shown. Reason: Frameshift at positions 587 and 606.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 855855Serine/threonine-protein kinase HAL5
PRO_0000085988

Regions

Domain503 – 837335Protein kinase
Nucleotide binding509 – 5179ATP By similarity
Compositional bias369 – 3779Poly-Gln

Sites

Active site6881Proton acceptor By similarity
Binding site5461ATP By similarity

Amino acid modifications

Modified residue171Phosphoserine Ref.8
Modified residue191Phosphoserine Ref.8
Modified residue681Phosphoserine Ref.6 Ref.9 Ref.10
Modified residue721Phosphoserine Ref.6 Ref.10
Modified residue1601Phosphoserine Ref.9
Modified residue2731Phosphoserine Ref.10
Modified residue2771Phosphoserine Ref.10
Modified residue3241Phosphoserine Ref.9 Ref.10
Modified residue3331Phosphoserine Ref.10
Modified residue3361Phosphoserine Ref.9 Ref.10
Modified residue3581Phosphoserine Ref.6
Modified residue3911Phosphoserine Ref.6
Modified residue3951Phosphoserine Ref.6 Ref.9 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P38970 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 4F23A97996489F39

FASTA85595,455
        10         20         30         40         50         60 
MGDEKLSRHT SLKRARSLSE SIKGLFKPSG ISGSNNAAAP SSRPGQDQAH SHQTARIITS 

        70         80         90        100        110        120 
NVSSPSISPV HSPVLQAAPK HHKLGVPNIA KLSLSPSREP SLNSENEMFS QESFISEKDE 

       130        140        150        160        170        180 
DEANLLERED LQNKKEEKAR AKHVRSKEAY VPHHRYTVGS DEVERQPRER LKNFPQNAGS 

       190        200        210        220        230        240 
SNPANSNANH VLDQENNFSI DAMLDYDEES KLRRRNSLGV RNHSNRTRSR KNSLSTPRSP 

       250        260        270        280        290        300 
PMKNGNGGMN SNATNNVGNG TGNRIYMRGR NHSDSISASS LPKFQEIECK CILDLGHFKV 

       310        320        330        340        350        360 
FENGYHEHSL RVLPIITNNK NVDSGDEKDA DASVNSGDDG DNDSEANMHK QKSVFSLSGL 

       370        380        390        400        410        420 
FKSHKDGNQQ QQQQQQQEEN GEQINLEKAF SIIPSQRFIK SQTLKKSRTS NLKNGNNDEL 

       430        440        450        460        470        480 
MKNDGKNIPQ IVNPNAAVGV EELKLINALS EKIRKGLKSE NTKGNNGEGR SNSNKQEDSD 

       490        500        510        520        530        540 
DTEGKAGTTN DDTSHKPCSQ KYGKSIGVVG AGAYGVVKIC ARCKTAKDVL PYSTYSNGKK 

       550        560        570        580        590        600 
LFFAVKELKP KPGDQIDKFC TRLTSEFIIG HSLSHPHFEA NAMIAGNVSR TTPPKHVFNA 

       610        620        630        640        650        660 
PNILKILDLM EYSNSFVEVM EFCASGDLYS LLTRNNISNE SNNGSSRLIQ TVKEGSGSPL 

       670        680        690        700        710        720 
HPLEADCFMK QLLNGVQYMH DHGIAHCDLK PENILFQPNG LLKICDFGTS SVFQTAWEKH 

       730        740        750        760        770        780 
VHFQSGAMGS EPYVAPEEFI RDAEYDPRLV DCWSCGIVYC TMVMGQYLWK IAIPEKDSLF 

       790        800        810        820        830        840 
KSFLSEIKDD GQFYLFEELR HVSSELNRLR KIALYRTFQV DPTKRITIEQ LLQSSWMRKT 

       850 
KCCVVYRHLH TKVSK 

« Hide

References

« Hide 'large scale' references
[1]"Saccharomyces cerevisiae HAL5 gene, mRNA."
Kron S.J.
Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A novel mechanism of ion homeostasis and salt tolerance in yeast: the Hal4 and Hal5 protein kinases modulate the Trk1-Trk2 potassium transporter."
Mulet J.M., Leube M.P., Kron S.J., Rios G., Fink G.R., Serrano R.
Mol. Cell. Biol. 19:3328-3337(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Yeast ARL1 encodes a regulator of K+ influx."
Munson A.M., Haydon D.H., Love S.L., Fell G.L., Palanivel V.R., Rosenwald A.G.
J. Cell Sci. 117:2309-2320(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-72; SER-358; SER-391 AND SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Key role for intracellular K+ and protein kinases Sat4/Hal4 and Hal5 in the plasma membrane stabilization of yeast nutrient transporters."
Perez-Valle J., Jenkins H., Merchan S., Montiel V., Ramos J., Sharma S., Serrano R., Yenush L.
Mol. Cell. Biol. 27:5725-5736(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17 AND SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-160; SER-324; SER-336 AND SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-72; SER-273; SER-277; SER-324; SER-333; SER-336 AND SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15300 mRNA. Translation: AAA50395.1. Frameshift.
Z49440 Genomic DNA. Translation: CAA89460.1.
BK006943 Genomic DNA. Translation: DAA08638.1.
PIRS56948.
RefSeqNP_012370.1. NM_001181598.1.

3D structure databases

ProteinModelPortalP38970.
SMRP38970. Positions 506-839.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33594. 153 interactions.
DIPDIP-6399N.
IntActP38970. 3 interactions.
MINTMINT-689966.
STRING4932.YJL165C.

Proteomic databases

MaxQBP38970.
PaxDbP38970.
PeptideAtlasP38970.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL165C; YJL165C; YJL165C.
GeneID853274.
KEGGsce:YJL165C.

Organism-specific databases

CYGDYJL165c.
SGDS000003701. HAL5.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000075309.
HOGENOMHOG000112846.
KOK08286.
OMAIANADEK.
OrthoDBEOG7008J9.

Enzyme and pathway databases

BioCycYEAST:G3O-31605-MONOMER.

Gene expression databases

GenevestigatorP38970.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973553.

Entry information

Entry nameHAL5_YEAST
AccessionPrimary (citable) accession number: P38970
Secondary accession number(s): D6VW22
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families