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P38970

- HAL5_YEAST

UniProt

P38970 - HAL5_YEAST

Protein

Serine/threonine-protein kinase HAL5

Gene

HAL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Protein kinase involved in salt tolerance and pH sensitivity, probably by regulating plasma membrane potential and cation influx. Positively controls the TRK1-TRK2 potassium transport system in response to potassium starvation. Stabilizes TRK1 in the plasma membrane by preventing its vacuolar sorting and degradation. Also stabilizes other plasma membrane nutrient transporters like CAN1, FUR4 and HXT1. May itself be subject to regulation by ARL1.3 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei546 – 5461ATPPROSITE-ProRule annotation
    Active sitei688 – 6881Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi509 – 5179ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase activity Source: SGD
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. cellular cation homeostasis Source: SGD
    2. cellular protein localization Source: SGD
    3. protein dephosphorylation Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31605-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase HAL5 (EC:2.7.11.1)
    Alternative name(s):
    Halotolerance protein 5
    Gene namesi
    Name:HAL5
    Ordered Locus Names:YJL165C
    ORF Names:J0531
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL165c.
    SGDiS000003701. HAL5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 855855Serine/threonine-protein kinase HAL5PRO_0000085988Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei17 – 171Phosphoserine1 Publication
    Modified residuei19 – 191Phosphoserine1 Publication
    Modified residuei68 – 681Phosphoserine3 Publications
    Modified residuei72 – 721Phosphoserine2 Publications
    Modified residuei160 – 1601Phosphoserine1 Publication
    Modified residuei273 – 2731Phosphoserine1 Publication
    Modified residuei277 – 2771Phosphoserine1 Publication
    Modified residuei324 – 3241Phosphoserine2 Publications
    Modified residuei333 – 3331Phosphoserine1 Publication
    Modified residuei336 – 3361Phosphoserine2 Publications
    Modified residuei358 – 3581Phosphoserine1 Publication
    Modified residuei391 – 3911Phosphoserine1 Publication
    Modified residuei395 – 3951Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38970.
    PaxDbiP38970.
    PeptideAtlasiP38970.

    Expressioni

    Gene expression databases

    GenevestigatoriP38970.

    Interactioni

    Protein-protein interaction databases

    BioGridi33594. 153 interactions.
    DIPiDIP-6399N.
    IntActiP38970. 3 interactions.
    MINTiMINT-689966.
    STRINGi4932.YJL165C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38970.
    SMRiP38970. Positions 506-839.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini503 – 837335Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi369 – 3779Poly-Gln

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000075309.
    HOGENOMiHOG000112846.
    KOiK08286.
    OMAiIANADEK.
    OrthoDBiEOG7008J9.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38970-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDEKLSRHT SLKRARSLSE SIKGLFKPSG ISGSNNAAAP SSRPGQDQAH    50
    SHQTARIITS NVSSPSISPV HSPVLQAAPK HHKLGVPNIA KLSLSPSREP 100
    SLNSENEMFS QESFISEKDE DEANLLERED LQNKKEEKAR AKHVRSKEAY 150
    VPHHRYTVGS DEVERQPRER LKNFPQNAGS SNPANSNANH VLDQENNFSI 200
    DAMLDYDEES KLRRRNSLGV RNHSNRTRSR KNSLSTPRSP PMKNGNGGMN 250
    SNATNNVGNG TGNRIYMRGR NHSDSISASS LPKFQEIECK CILDLGHFKV 300
    FENGYHEHSL RVLPIITNNK NVDSGDEKDA DASVNSGDDG DNDSEANMHK 350
    QKSVFSLSGL FKSHKDGNQQ QQQQQQQEEN GEQINLEKAF SIIPSQRFIK 400
    SQTLKKSRTS NLKNGNNDEL MKNDGKNIPQ IVNPNAAVGV EELKLINALS 450
    EKIRKGLKSE NTKGNNGEGR SNSNKQEDSD DTEGKAGTTN DDTSHKPCSQ 500
    KYGKSIGVVG AGAYGVVKIC ARCKTAKDVL PYSTYSNGKK LFFAVKELKP 550
    KPGDQIDKFC TRLTSEFIIG HSLSHPHFEA NAMIAGNVSR TTPPKHVFNA 600
    PNILKILDLM EYSNSFVEVM EFCASGDLYS LLTRNNISNE SNNGSSRLIQ 650
    TVKEGSGSPL HPLEADCFMK QLLNGVQYMH DHGIAHCDLK PENILFQPNG 700
    LLKICDFGTS SVFQTAWEKH VHFQSGAMGS EPYVAPEEFI RDAEYDPRLV 750
    DCWSCGIVYC TMVMGQYLWK IAIPEKDSLF KSFLSEIKDD GQFYLFEELR 800
    HVSSELNRLR KIALYRTFQV DPTKRITIEQ LLQSSWMRKT KCCVVYRHLH 850
    TKVSK 855
    Length:855
    Mass (Da):95,455
    Last modified:November 1, 1995 - v2
    Checksum:i4F23A97996489F39
    GO

    Sequence cautioni

    The sequence AAA50395.1 differs from that shown. Reason: Frameshift at positions 587 and 606.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15300 mRNA. Translation: AAA50395.1. Frameshift.
    Z49440 Genomic DNA. Translation: CAA89460.1.
    BK006943 Genomic DNA. Translation: DAA08638.1.
    PIRiS56948.
    RefSeqiNP_012370.1. NM_001181598.1.

    Genome annotation databases

    EnsemblFungiiYJL165C; YJL165C; YJL165C.
    GeneIDi853274.
    KEGGisce:YJL165C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15300 mRNA. Translation: AAA50395.1 . Frameshift.
    Z49440 Genomic DNA. Translation: CAA89460.1 .
    BK006943 Genomic DNA. Translation: DAA08638.1 .
    PIRi S56948.
    RefSeqi NP_012370.1. NM_001181598.1.

    3D structure databases

    ProteinModelPortali P38970.
    SMRi P38970. Positions 506-839.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33594. 153 interactions.
    DIPi DIP-6399N.
    IntActi P38970. 3 interactions.
    MINTi MINT-689966.
    STRINGi 4932.YJL165C.

    Proteomic databases

    MaxQBi P38970.
    PaxDbi P38970.
    PeptideAtlasi P38970.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL165C ; YJL165C ; YJL165C .
    GeneIDi 853274.
    KEGGi sce:YJL165C.

    Organism-specific databases

    CYGDi YJL165c.
    SGDi S000003701. HAL5.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000075309.
    HOGENOMi HOG000112846.
    KOi K08286.
    OMAi IANADEK.
    OrthoDBi EOG7008J9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31605-MONOMER.

    Miscellaneous databases

    NextBioi 973553.

    Gene expression databases

    Genevestigatori P38970.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Saccharomyces cerevisiae HAL5 gene, mRNA."
      Kron S.J.
      Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A novel mechanism of ion homeostasis and salt tolerance in yeast: the Hal4 and Hal5 protein kinases modulate the Trk1-Trk2 potassium transporter."
      Mulet J.M., Leube M.P., Kron S.J., Rios G., Fink G.R., Serrano R.
      Mol. Cell. Biol. 19:3328-3337(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: FUNCTION.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-72; SER-358; SER-391 AND SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Key role for intracellular K+ and protein kinases Sat4/Hal4 and Hal5 in the plasma membrane stabilization of yeast nutrient transporters."
      Perez-Valle J., Jenkins H., Merchan S., Montiel V., Ramos J., Sharma S., Serrano R., Yenush L.
      Mol. Cell. Biol. 27:5725-5736(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17 AND SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-160; SER-324; SER-336 AND SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-72; SER-273; SER-277; SER-324; SER-333; SER-336 AND SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHAL5_YEAST
    AccessioniPrimary (citable) accession number: P38970
    Secondary accession number(s): D6VW22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3