Serine/threonine-protein kinase HAL5 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P38970 (HAL5_YEAST)

Last modified November 24, 2009. Version 87. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase HAL5
    EC=2.7.11.1
Alternative name(s):
    Halotolerance protein 5

Gene names

Name:	HAL5
Ordered Locus Names:	YJL165C
ORF Names:	J0531

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	855 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Protein kinase involved in salt tolerance and pH sensitivity, probably by regulating plasma membrane potential and cation influx. Positively controls the TRK1-TRK2 potassium transport system in response to potassium starvation. Stabilizes TRK1 in the plasma membrane by preventing its vacuolar sorting and degradation. Also stabilizes other plasma membrane nutrient transporters like CAN1, FUR4 and HXT1. May itself be subjet to regulation by ARL1. Ref.3 Ref.4 Ref.6
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NPR/HAL subfamily. HAL5 sub-subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAA50395.1 differs from that shown. Reason: Frameshift at positions 587 and 606.

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Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cation homeostasis Ref.3 Inferred from genetic interaction. Source: SGD protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
ITR2	P30606	1	EBI-8142,EBI-9395

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 855

855

Serine/threonine-protein kinase HAL5

PRO_0000085988

Regions

Domain

503 – 837

335

Protein kinase

Nucleotide binding

509 – 517

ATP By similarity

Compositional bias

369 – 377

Poly-Gln

Sites

Active site

688

Proton acceptor By similarity

Binding site

546

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Modified residue

Phosphothreonine Ref.7

Modified residue

Phosphoserine Ref.7 Ref.8

Modified residue

Phosphoserine Ref.7 Ref.8

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.9 Ref.5

Modified residue

Phosphoserine Ref.9 Ref.5

Modified residue

160

Phosphoserine Ref.9

Modified residue

217

Phosphoserine Ref.8

Modified residue

233

Phosphoserine Ref.8 Ref.9

Modified residue

273

Phosphoserine Ref.8 Ref.9

Modified residue

280

Phosphoserine Ref.9

Modified residue

324

Phosphoserine Ref.9

Modified residue

336

Phosphoserine Ref.9

Modified residue

358

Phosphoserine Ref.5

Modified residue

391

Phosphoserine Ref.9 Ref.5

Modified residue

395

Phosphoserine Ref.9 Ref.5

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Sequences

Sequence

Length

Mass (Da)

Tools

P38970-1 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 4F23A97996489F39
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FASTA

855

95,455

        10         20         30         40         50         60 
MGDEKLSRHT SLKRARSLSE SIKGLFKPSG ISGSNNAAAP SSRPGQDQAH SHQTARIITS 

        70         80         90        100        110        120 
NVSSPSISPV HSPVLQAAPK HHKLGVPNIA KLSLSPSREP SLNSENEMFS QESFISEKDE 

       130        140        150        160        170        180 
DEANLLERED LQNKKEEKAR AKHVRSKEAY VPHHRYTVGS DEVERQPRER LKNFPQNAGS 

       190        200        210        220        230        240 
SNPANSNANH VLDQENNFSI DAMLDYDEES KLRRRNSLGV RNHSNRTRSR KNSLSTPRSP 

       250        260        270        280        290        300 
PMKNGNGGMN SNATNNVGNG TGNRIYMRGR NHSDSISASS LPKFQEIECK CILDLGHFKV 

       310        320        330        340        350        360 
FENGYHEHSL RVLPIITNNK NVDSGDEKDA DASVNSGDDG DNDSEANMHK QKSVFSLSGL 

       370        380        390        400        410        420 
FKSHKDGNQQ QQQQQQQEEN GEQINLEKAF SIIPSQRFIK SQTLKKSRTS NLKNGNNDEL 

       430        440        450        460        470        480 
MKNDGKNIPQ IVNPNAAVGV EELKLINALS EKIRKGLKSE NTKGNNGEGR SNSNKQEDSD 

       490        500        510        520        530        540 
DTEGKAGTTN DDTSHKPCSQ KYGKSIGVVG AGAYGVVKIC ARCKTAKDVL PYSTYSNGKK 

       550        560        570        580        590        600 
LFFAVKELKP KPGDQIDKFC TRLTSEFIIG HSLSHPHFEA NAMIAGNVSR TTPPKHVFNA 

       610        620        630        640        650        660 
PNILKILDLM EYSNSFVEVM EFCASGDLYS LLTRNNISNE SNNGSSRLIQ TVKEGSGSPL 

       670        680        690        700        710        720 
HPLEADCFMK QLLNGVQYMH DHGIAHCDLK PENILFQPNG LLKICDFGTS SVFQTAWEKH 

       730        740        750        760        770        780 
VHFQSGAMGS EPYVAPEEFI RDAEYDPRLV DCWSCGIVYC TMVMGQYLWK IAIPEKDSLF 

       790        800        810        820        830        840 
KSFLSEIKDD GQFYLFEELR HVSSELNRLR KIALYRTFQV DPTKRITIEQ LLQSSWMRKT 

       850 
KCCVVYRHLH TKVSK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Saccharomyces cerevisiae HAL5 gene, mRNA." Kron S.J. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"A novel mechanism of ion homeostasis and salt tolerance in yeast: the Hal4 and Hal5 protein kinases modulate the Trk1-Trk2 potassium transporter." Mulet J.M., Leube M.P., Kron S.J., Rios G., Fink G.R., Serrano R. Mol. Cell. Biol. 19:3328-3337(1999) [PubMed: 10207057] [Abstract] Cited for: FUNCTION.
[4]	"Yeast ARL1 encodes a regulator of K+ influx." Munson A.M., Haydon D.H., Love S.L., Fell G.L., Palanivel V.R., Rosenwald A.G. J. Cell Sci. 117:2309-2320(2004) [PubMed: 15126631] [Abstract] Cited for: FUNCTION.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-72; SER-358; SER-391 AND SER-395, MASS SPECTROMETRY.
[6]	"Key role for intracellular K+ and protein kinases Sat4/Hal4 and Hal5 in the plasma membrane stabilization of yeast nutrient transporters." Perez-Valle J., Jenkins H., Merchan S., Montiel V., Ramos J., Sharma S., Serrano R., Yenush L. Mol. Cell. Biol. 27:5725-5736(2007) [PubMed: 17548466] [Abstract] Cited for: FUNCTION.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7; THR-10; SER-17 AND SER-19, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-19; SER-217; SER-233 AND SER-273, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63; SER-68; SER-72; SER-160; SER-233; SER-273; SER-280; SER-324; SER-336; SER-391 AND SER-395, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U15300 mRNA. Translation: AAA50395.1. Frameshift. Z49440 Genomic DNA. Translation: CAA89460.1.
PIR	S56948.
RefSeq	NP_012370.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6399N.
IntAct	P38970. 3 interactions.
STRING	P38970.
Proteomic databases
PeptideAtlas	P38970.
PRIDE	P38970.
Genome annotation databases
Ensembl	YJL165C; YJL165C; YJL165C; Saccharomyces cerevisiae. [Genome view]
GeneID	853274.
KEGG	sce:YJL165C.
NMPDR	fig\|4932.3.peg.3334.
Organism-specific databases
CYGD	YJL165c.
SGD	S000003701. HAL5.
Phylogenomic databases
HOGENOM	P38970.
OMA	IANADEK
OrthoDB	EOG9QVDVF
Enzyme and pathway databases
BRENDA	2.7.11.1. 250.
Gene expression databases
ArrayExpress	P38970.
Genevestigator	P38970.
GermOnline	YJL165C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973553.

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Entry information

Entry name

HAL5_YEAST

Accession

Primary (citable) accession number: P38970

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents