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Protein

Tryptophan permease

Gene

TAT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for high-affinity tryptophan transport. Also transports cysteine, phenyalanine and tyrosine.2 Publications

GO - Molecular functioni

  • aromatic amino acid transmembrane transporter activity Source: SGD
  • catalytic activity Source: InterPro
  • high-affinity tryptophan transmembrane transporter activity Source: SGD

GO - Biological processi

  • aromatic amino acid transport Source: SGD
  • tryptophan transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33436-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.8. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan permease
Alternative name(s):
Tryptophan amino acid transporter
Gene namesi
Name:TAT2
Synonyms:LTG3, SAB2, SCM2, TAP2
Ordered Locus Names:YOL020W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL020W.
SGDiS000005380. TAT2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8787CytoplasmicSequence analysisAdd
BLAST
Transmembranei88 – 10821HelicalSequence analysisAdd
BLAST
Topological domaini109 – 1135ExtracellularSequence analysis
Transmembranei114 – 13421HelicalSequence analysisAdd
BLAST
Topological domaini135 – 16026CytoplasmicSequence analysisAdd
BLAST
Transmembranei161 – 18121HelicalSequence analysisAdd
BLAST
Topological domaini182 – 19211ExtracellularSequence analysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence analysisAdd
BLAST
Topological domaini214 – 22613CytoplasmicSequence analysisAdd
BLAST
Transmembranei227 – 24721HelicalSequence analysisAdd
BLAST
Topological domaini248 – 26619ExtracellularSequence analysisAdd
BLAST
Transmembranei267 – 28721HelicalSequence analysisAdd
BLAST
Topological domaini288 – 30619CytoplasmicSequence analysisAdd
BLAST
Transmembranei307 – 32721HelicalSequence analysisAdd
BLAST
Topological domaini328 – 35831ExtracellularSequence analysisAdd
BLAST
Transmembranei359 – 37921HelicalSequence analysisAdd
BLAST
Topological domaini380 – 40425CytoplasmicSequence analysisAdd
BLAST
Transmembranei405 – 42521HelicalSequence analysisAdd
BLAST
Topological domaini426 – 4283ExtracellularSequence analysis
Transmembranei429 – 44921HelicalSequence analysisAdd
BLAST
Topological domaini450 – 47223CytoplasmicSequence analysisAdd
BLAST
Transmembranei473 – 49321HelicalSequence analysisAdd
BLAST
Topological domaini494 – 51421ExtracellularSequence analysisAdd
BLAST
Transmembranei515 – 53521HelicalSequence analysisAdd
BLAST
Topological domaini536 – 59257CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 592592Tryptophan permeasePRO_0000054160Add
BLAST

Proteomic databases

MaxQBiP38967.
TopDownProteomicsiP38967.

PTM databases

SwissPalmiP38967.

Interactioni

Protein-protein interaction databases

BioGridi34382. 52 interactions.
DIPiDIP-1782N.
IntActiP38967. 4 interactions.
MINTiMINT-408126.

Structurei

3D structure databases

ProteinModelPortaliP38967.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP38967.
KOiK16261.
OMAiNSCIFAS.
OrthoDBiEOG74J9HZ.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 4 hits.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38967-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEDFISSVK RSNEELKERK SNFGFVEYKS KQLTSSSSHN SNSSHHDDDN
60 70 80 90 100
QHGKRNIFQR CVDSFKSPLD GSFDTSNLKR TLKPRHLIMI AIGGSIGTGL
110 120 130 140 150
FVGSGKAIAE GGPLGVVIGW AIAGSQIIGT IHGLGEITVR FPVVGAFANY
160 170 180 190 200
GTRFLDPSIS FVVSTIYVLQ WFFVLPLEII AAAMTVQYWN SSIDPVIWVA
210 220 230 240 250
IFYAVIVSIN LFGVRGFGEA EFAFSTIKAI TVCGFIILCV VLICGGGPDH
260 270 280 290 300
EFIGAKYWHD PGCLANGFPG VLSVLVVASY SLGGIEMTCL ASGETDPKGL
310 320 330 340 350
PSAIKQVFWR ILFFFLISLT LVGFLVPYTN QNLLGGSSVD NSPFVIAIKL
360 370 380 390 400
HHIKALPSIV NAVILISVLS VGNSCIFASS RTLCSMAHQG LIPWWFGYID
410 420 430 440 450
RAGRPLVGIM ANSLFGLLAF LVKSGSMSEV FNWLMAIAGL ATCIVWLSIN
460 470 480 490 500
LSHIRFRLAM KAQGKSLDEL EFVSAVGIWG SAYSALINCL ILIAQFYCSL
510 520 530 540 550
WPIGGWTSGK ERAKIFFQNY LCALIMLFIF IVHKIYYKCQ TGKWWGVKAL
560 570 580 590
KDIDLETDRK DIDIEIVKQE IAEKKMYLDS RPWYVRQFHF WC
Length:592
Mass (Da):65,404
Last modified:February 1, 1995 - v1
Checksum:iA233BAD16264B319
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1871Q → T in BAA03811 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79150 Genomic DNA. Translation: CAA55777.1.
L33461 Genomic DNA. Translation: AAA60324.1.
D16304 Genomic DNA. Translation: BAA03811.1.
U66834 Genomic DNA. Translation: AAB07526.1.
Z74761 Genomic DNA. Translation: CAA99019.1.
Z74762 Genomic DNA. Translation: CAA99020.1.
BK006948 Genomic DNA. Translation: DAA10761.1.
PIRiS46273.
RefSeqiNP_014622.1. NM_001183274.1.

Genome annotation databases

EnsemblFungiiYOL020W; YOL020W; YOL020W.
GeneIDi854139.
KEGGisce:YOL020W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79150 Genomic DNA. Translation: CAA55777.1.
L33461 Genomic DNA. Translation: AAA60324.1.
D16304 Genomic DNA. Translation: BAA03811.1.
U66834 Genomic DNA. Translation: AAB07526.1.
Z74761 Genomic DNA. Translation: CAA99019.1.
Z74762 Genomic DNA. Translation: CAA99020.1.
BK006948 Genomic DNA. Translation: DAA10761.1.
PIRiS46273.
RefSeqiNP_014622.1. NM_001183274.1.

3D structure databases

ProteinModelPortaliP38967.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34382. 52 interactions.
DIPiDIP-1782N.
IntActiP38967. 4 interactions.
MINTiMINT-408126.

Protein family/group databases

TCDBi2.A.3.10.8. the amino acid-polyamine-organocation (apc) family.

PTM databases

SwissPalmiP38967.

Proteomic databases

MaxQBiP38967.
TopDownProteomicsiP38967.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL020W; YOL020W; YOL020W.
GeneIDi854139.
KEGGisce:YOL020W.

Organism-specific databases

EuPathDBiFungiDB:YOL020W.
SGDiS000005380. TAT2.

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP38967.
KOiK16261.
OMAiNSCIFAS.
OrthoDBiEOG74J9HZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-33436-MONOMER.

Miscellaneous databases

NextBioi975874.
PROiP38967.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 4 hits.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two FK506 resistance-conferring genes in Saccharomyces cerevisiae, TAT1 and TAT2, encode amino acid permeases mediating tyrosine and tryptophan uptake."
    Schmidt A., Hall M.N., Koller A.
    Mol. Cell. Biol. 14:6597-6606(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JK9-3D.
  2. "SCM2, a tryptophan permease in Saccharomyces cerevisiae, is important for cell growth."
    Chen X.H., Xiao Z., Fitzgerald-Hayes M.
    Mol. Gen. Genet. 244:260-268(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning and nucleotide sequence of a gene conferring ability to grow at a low temperature on Saccharomyces cerevisiae tryptophan auxotroph."
    Kawamura D., Yamashita I., Nimi O., Toh-e A.
    J. Ferment. Bioeng. 77:1-9(1994)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: IFO 10151 / YNN140.
  4. Shin Y.H., Goo D.M., So I.S., Rhode P.R., Campbell J.L., Kim J.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "Cysteine uptake by Saccharomyces cerevisiae is accomplished by multiple permeases."
    During-Olsen L., Regenberg B., Gjermansen C., Kielland-Brandt M.C., Hansen J.
    Curr. Genet. 35:609-617(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN L-CYSTEINE UPTAKE.
  8. "Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae."
    Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S.
    Curr. Genet. 36:317-328(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiTAT2_YEAST
AccessioniPrimary (citable) accession number: P38967
Secondary accession number(s): D6W245
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.