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Protein

25S rRNA (adenine(645)-N(1))-methyltransferase

Gene

RRP8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N1 position of adenine 645 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2. Also involved in telomere length regulation and maintenance.4 Publications

Catalytic activityi

S-adenosyl-L-methionine + adenine(645) in 25S rRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(645) in 25S rRNA.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei156 – 1561S-adenosyl-L-methionineBy similarity
Binding sitei207 – 2071S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei242 – 2421S-adenosyl-L-methionineBy similarity
Binding sitei254 – 2541S-adenosyl-L-methionineBy similarity
Binding sitei271 – 2711S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

  • rRNA (adenine) methyltransferase activity Source: SGD

GO - Biological processi

  • ribosomal large subunit biogenesis Source: SGD
  • rRNA methylation Source: SGD
  • rRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29688-MONOMER.
BRENDAi2.1.1.287. 984.
ReactomeiR-SCE-427359. SIRT1 negatively regulates rRNA Expression.

Names & Taxonomyi

Protein namesi
Recommended name:
25S rRNA (adenine(645)-N(1))-methyltransferase (EC:2.1.1.287)
Alternative name(s):
Ribosomal RNA-processing protein 8
Gene namesi
Name:RRP8
Ordered Locus Names:YDR083W
ORF Names:D4461
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR083W.
SGDiS000002490. RRP8.

Subcellular locationi

GO - Cellular componenti

  • 90S preribosome Source: GO_Central
  • chromosome, telomeric region Source: UniProtKB-SubCell
  • nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi209 – 2091G → A or R: Impaired methyltransferase activity and ribosomal 60S subunit biogenesis. Does not affect pre-rRNA cleavage at site A2 activity. 1 Publication
Mutagenesisi390 – 3901K → E in RRP8-1; no effect. Synthetic lethal with GAR1 mutant allele lacking its N- and C-terminal glycine/arginine-rich (GAR) domains. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 39239225S rRNA (adenine(645)-N(1))-methyltransferasePRO_0000202594Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38961.
PRIDEiP38961.

PTM databases

iPTMnetiP38961.

Interactioni

Protein-protein interaction databases

BioGridi32139. 115 interactions.
IntActiP38961. 4 interactions.
MINTiMINT-4480662.

Structurei

3D structure databases

ProteinModelPortaliP38961.
SMRiP38961. Positions 120-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili32 – 6130Sequence analysisAdd
BLAST
Coiled coili344 – 38239Sequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000006189.
HOGENOMiHOG000164004.
InParanoidiP38961.
KOiK14850.
OMAiGQWLLKP.
OrthoDBiEOG7WX0KP.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR007823. RRP8.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR12787. PTHR12787. 3 hits.
PfamiPF05148. Methyltransf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P38961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALFNVEGWS IKTKTVAFDN KTNKSSKDKK KNNRKNGKLT REQKLKEETE
60 70 80 90 100
AELKEQVEDI PSEGSVAKDI PKKNQEKSDQ NETSKKRKHD EEAPLMQVKE
110 120 130 140 150
NIEKPTKKQL TPLQQKMMAK LTGSRFRWIN EQLYTISSDE ALKLIKEQPQ
160 170 180 190 200
LFDEYHDGFR SQVQAWPENP VDVFVDQIRY RCMKPVNAPG GLPGLKDSKE
210 220 230 240 250
IVIADMGCGE AQLALEINNF FKNYNKKAKK YLKRRHKVHS FDLKKANERI
260 270 280 290 300
TVADIRNVPL PDESCTIVVF CLALMGTNFL DFIKEAYRIL APRGELWIAE
310 320 330 340 350
IKSRFSDGKG NEFVDALKLM GFFHKKTFDE NKMFTRFEFF KPPAEIIEER
360 370 380 390
RQKLERRQKF IEVETEKEEL EKKRRKIAEG KWLLKPCIYK RR
Length:392
Mass (Da):45,985
Last modified:October 31, 2006 - v2
Checksum:i58675E3493D9B57B
GO

Sequence cautioni

The sequence CAA57610.1 differs from that shown. Reason: Frameshift at position 380. Curated
The sequence CAA86805.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA98903.1 differs from that shown. Reason: Frameshift at position 380. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82086 Genomic DNA. Translation: CAA57610.1. Frameshift.
Z46796 Genomic DNA. Translation: CAA86805.1. Different initiation.
Z74379 Genomic DNA. Translation: CAA98903.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA11930.1.
PIRiS48770.
RefSeqiNP_010368.5. NM_001180391.3.

Genome annotation databases

EnsemblFungiiYDR083W; YDR083W; YDR083W.
GeneIDi851656.
KEGGisce:YDR083W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82086 Genomic DNA. Translation: CAA57610.1. Frameshift.
Z46796 Genomic DNA. Translation: CAA86805.1. Different initiation.
Z74379 Genomic DNA. Translation: CAA98903.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA11930.1.
PIRiS48770.
RefSeqiNP_010368.5. NM_001180391.3.

3D structure databases

ProteinModelPortaliP38961.
SMRiP38961. Positions 120-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32139. 115 interactions.
IntActiP38961. 4 interactions.
MINTiMINT-4480662.

PTM databases

iPTMnetiP38961.

Proteomic databases

MaxQBiP38961.
PRIDEiP38961.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR083W; YDR083W; YDR083W.
GeneIDi851656.
KEGGisce:YDR083W.

Organism-specific databases

EuPathDBiFungiDB:YDR083W.
SGDiS000002490. RRP8.

Phylogenomic databases

GeneTreeiENSGT00390000006189.
HOGENOMiHOG000164004.
InParanoidiP38961.
KOiK14850.
OMAiGQWLLKP.
OrthoDBiEOG7WX0KP.

Enzyme and pathway databases

BioCyciYEAST:G3O-29688-MONOMER.
BRENDAi2.1.1.287. 984.
ReactomeiR-SCE-427359. SIRT1 negatively regulates rRNA Expression.

Miscellaneous databases

PROiP38961.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR007823. RRP8.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR12787. PTHR12787. 3 hits.
PfamiPF05148. Methyltransf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2."
    Bousquet-Antonelli C., Vanrobays E., Gelugne J.P., Caizergues-Ferrer M., Henry Y.
    RNA 6:826-843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-390.
    Strain: JG535-2D.
  2. "Analysis of a 32.8 kb segment of yeast chromosome IV reveals 21 open reading frames, including TPS2, PPH3, RAD55, SED1, PDC2, AFR1, SSS1, SLU7 and a tRNA for arginine."
    Coster F., Jonniaux J.-L., Goffeau A.
    Yeast 11:673-679(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length."
    Askree S.H., Yehuda T., Smolikov S., Gurevich R., Hawk J., Coker C., Krauskopf A., Kupiec M., McEachern M.J.
    Proc. Natl. Acad. Sci. U.S.A. 101:8658-8663(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Telomere length as a quantitative trait: genome-wide survey and genetic mapping of telomere length-control genes in yeast."
    Gatbonton T., Imbesi M., Nelson M., Akey J.M., Ruderfer D.M., Kruglyak L., Simon J.A., Bedalov A.
    PLoS Genet. 2:304-315(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA."
    Peifer C., Sharma S., Watzinger P., Lamberth S., Kotter P., Entian K.D.
    Nucleic Acids Res. 41:1151-1163(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-209.

Entry informationi

Entry nameiRRP8_YEAST
AccessioniPrimary (citable) accession number: P38961
Secondary accession number(s): Q7LHG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 31, 2006
Last modified: July 6, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2780 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.