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Protein

25S rRNA (adenine(645)-N(1))-methyltransferase

Gene

RRP8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N1 position of adenine 645 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2. Also involved in telomere length regulation and maintenance.4 Publications

Miscellaneous

Present with 2780 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + adenine(645) in 25S rRNA = S-adenosyl-L-homocysteine + N1-methyladenine(645) in 25S rRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei156S-adenosyl-L-methionineBy similarity1
Binding sitei207S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei242S-adenosyl-L-methionineBy similarity1
Binding sitei254S-adenosyl-L-methionineBy similarity1
Binding sitei271S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • rRNA (adenine) methyltransferase activity Source: SGD

GO - Biological processi

  • ribosomal large subunit biogenesis Source: SGD
  • rRNA methylation Source: SGD
  • rRNA processing Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29688-MONOMER
BRENDAi2.1.1.287 984
ReactomeiR-SCE-427359 SIRT1 negatively regulates rRNA expression

Names & Taxonomyi

Protein namesi
Recommended name:
25S rRNA (adenine(645)-N(1))-methyltransferase (EC:2.1.1.287)
Alternative name(s):
Ribosomal RNA-processing protein 8
Gene namesi
Name:RRP8
Ordered Locus Names:YDR083W
ORF Names:D4461
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR083W
SGDiS000002490 RRP8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi209G → A or R: Impaired methyltransferase activity and ribosomal 60S subunit biogenesis. Does not affect pre-rRNA cleavage at site A2 activity. 1 Publication1
Mutagenesisi390K → E in RRP8-1; no effect. Synthetic lethal with GAR1 mutant allele lacking its N- and C-terminal glycine/arginine-rich (GAR) domains. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002025941 – 39225S rRNA (adenine(645)-N(1))-methyltransferaseAdd BLAST392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei62PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38961
PaxDbiP38961
PRIDEiP38961

PTM databases

iPTMnetiP38961

Interactioni

Protein-protein interaction databases

BioGridi32139, 305 interactors
IntActiP38961, 4 interactors
STRINGi4932.YDR083W

Structurei

3D structure databases

ProteinModelPortaliP38961
SMRiP38961
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili32 – 61Sequence analysisAdd BLAST30
Coiled coili344 – 382Sequence analysisAdd BLAST39

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000006189
HOGENOMiHOG000164004
InParanoidiP38961
KOiK14850
OMAiAYRILHW
OrthoDBiEOG092C2Q7B

Family and domain databases

InterProiView protein in InterPro
IPR007823 RRP8
IPR029063 SAM-dependent_MTases
PANTHERiPTHR12787 PTHR12787, 1 hit
PfamiView protein in Pfam
PF05148 Methyltransf_8, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

P38961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALFNVEGWS IKTKTVAFDN KTNKSSKDKK KNNRKNGKLT REQKLKEETE
60 70 80 90 100
AELKEQVEDI PSEGSVAKDI PKKNQEKSDQ NETSKKRKHD EEAPLMQVKE
110 120 130 140 150
NIEKPTKKQL TPLQQKMMAK LTGSRFRWIN EQLYTISSDE ALKLIKEQPQ
160 170 180 190 200
LFDEYHDGFR SQVQAWPENP VDVFVDQIRY RCMKPVNAPG GLPGLKDSKE
210 220 230 240 250
IVIADMGCGE AQLALEINNF FKNYNKKAKK YLKRRHKVHS FDLKKANERI
260 270 280 290 300
TVADIRNVPL PDESCTIVVF CLALMGTNFL DFIKEAYRIL APRGELWIAE
310 320 330 340 350
IKSRFSDGKG NEFVDALKLM GFFHKKTFDE NKMFTRFEFF KPPAEIIEER
360 370 380 390
RQKLERRQKF IEVETEKEEL EKKRRKIAEG KWLLKPCIYK RR
Length:392
Mass (Da):45,985
Last modified:October 31, 2006 - v2
Checksum:i58675E3493D9B57B
GO

Sequence cautioni

The sequence CAA57610 differs from that shown. Reason: Frameshift at position 380.Curated
The sequence CAA86805 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA98903 differs from that shown. Reason: Frameshift at position 380.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82086 Genomic DNA Translation: CAA57610.1 Frameshift.
Z46796 Genomic DNA Translation: CAA86805.1 Different initiation.
Z74379 Genomic DNA Translation: CAA98903.1 Frameshift.
BK006938 Genomic DNA Translation: DAA11930.1
PIRiS48770
RefSeqiNP_010368.5, NM_001180391.3

Genome annotation databases

EnsemblFungiiYDR083W; YDR083W; YDR083W
GeneIDi851656
KEGGisce:YDR083W

Similar proteinsi

Entry informationi

Entry nameiRRP8_YEAST
AccessioniPrimary (citable) accession number: P38961
Secondary accession number(s): Q7LHG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 31, 2006
Last modified: May 23, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health