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Protein

DNA repair protein RAD55

Gene

RAD55

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for radiation resistance and meiotic viability and presumably acts in recombination and recombinational DNA repair pathways.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 508ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: InterPro
  • DNA-dependent ATPase activity Source: SGD
  • protein heterodimerization activity Source: SGD

GO - Biological processi

  • DNA recombinase assembly Source: SGD
  • heteroduplex formation Source: SGD
  • meiotic DNA recombinase assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29681-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD55
Gene namesi
Name:RAD55
Ordered Locus Names:YDR076W
ORF Names:D4426
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR076W.
SGDiS000002483. RAD55.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • Rhp55-Rhp57 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406DNA repair protein RAD55PRO_0000122954Add
BLAST

Proteomic databases

MaxQBiP38953.

PTM databases

iPTMnetiP38953.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD57P253012EBI-14737,EBI-14744

GO - Molecular functioni

  • protein heterodimerization activity Source: SGD

Protein-protein interaction databases

BioGridi32131. 159 interactions.
DIPiDIP-1554N.
IntActiP38953. 3 interactions.
MINTiMINT-386077.

Structurei

3D structure databases

ProteinModelPortaliP38953.
SMRiP38953. Positions 35-138.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi277 – 28711Ser-richAdd
BLAST
Compositional biasi371 – 3788Ser-rich

Sequence similaritiesi

Belongs to the RecA family. RAD55 subfamily.Curated

Phylogenomic databases

InParanoidiP38953.
KOiK10957.
OMAiANIASFP.
OrthoDBiEOG7SV16X.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013632. DNA_recomb/repair_Rad51_C.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38953-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLGIPLSQL IVESPKPLSS GITGLDEILN LGFQARSIYE IFGPPGIGKT
60 70 80 90 100
NFGIQLVCNS LEGIQQSEIN DDKILWIETF QEMPINILRE RFQKFKIVEE
110 120 130 140 150
NVKRVRITKF GQLLYFFQNL FKLSQSVRYK LVIIDGFSQL VCDHLCTLSK
160 170 180 190 200
RGGGMIDKTI HELKCRHLIL IFTVMTKYTH STGSTIIVLN DCMNTAFQSN
210 220 230 240 250
EFESLEEYYE ILDDGSNFFV NSNNERRKNN VHILKSALVA NIAMGSKDST
260 270 280 290 300
WEVFLRDRIG LFRDWNEQVD ETVFVKSKRV KASSSQSNEG CTTIKEMRIN
310 320 330 340 350
KRNFENLRIA IVFNLHGEDR KREGRNLKRS RSSDDRNYIV KFDFDKATGQ
360 370 380 390 400
LRDIIDLKPD TANIASFPTL STSSSSCSQV FNNIDSNDNP LPNAEGKEEI

IYDSEG
Length:406
Mass (Da):46,350
Last modified:February 1, 1995 - v1
Checksum:i8EA49831F7F3E098
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101F → L in AAA19688 (Ref. 2) Curated
Sequence conflicti197 – 1971F → N in AAA19688 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46796 Genomic DNA. Translation: CAA86798.1.
D10481 Genomic DNA. Translation: BAA01284.1.
U01144 Genomic DNA. Translation: AAA19688.1.
X82086 Genomic DNA. Translation: CAA57603.1.
Z74372 Genomic DNA. Translation: CAA98895.1.
AY723773 Genomic DNA. Translation: AAU09690.1.
BK006938 Genomic DNA. Translation: DAA11922.1.
PIRiS48763.
RefSeqiNP_010361.3. NM_001180384.3.

Genome annotation databases

EnsemblFungiiYDR076W; YDR076W; YDR076W.
GeneIDi851648.
KEGGisce:YDR076W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46796 Genomic DNA. Translation: CAA86798.1.
D10481 Genomic DNA. Translation: BAA01284.1.
U01144 Genomic DNA. Translation: AAA19688.1.
X82086 Genomic DNA. Translation: CAA57603.1.
Z74372 Genomic DNA. Translation: CAA98895.1.
AY723773 Genomic DNA. Translation: AAU09690.1.
BK006938 Genomic DNA. Translation: DAA11922.1.
PIRiS48763.
RefSeqiNP_010361.3. NM_001180384.3.

3D structure databases

ProteinModelPortaliP38953.
SMRiP38953. Positions 35-138.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32131. 159 interactions.
DIPiDIP-1554N.
IntActiP38953. 3 interactions.
MINTiMINT-386077.

PTM databases

iPTMnetiP38953.

Proteomic databases

MaxQBiP38953.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR076W; YDR076W; YDR076W.
GeneIDi851648.
KEGGisce:YDR076W.

Organism-specific databases

EuPathDBiFungiDB:YDR076W.
SGDiS000002483. RAD55.

Phylogenomic databases

InParanoidiP38953.
KOiK10957.
OMAiANIASFP.
OrthoDBiEOG7SV16X.

Enzyme and pathway databases

BioCyciYEAST:G3O-29681-MONOMER.

Miscellaneous databases

PROiP38953.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013632. DNA_recomb/repair_Rad51_C.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the RAD55 gene of Saccharomyces cerevisiae: similarity of RAD55 to prokaryotic RecA and other RecA-like proteins."
    Lovett S.T.
    Gene 142:103-106(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204510 / AB320.
  2. "Cloning of the RAD55 gene and identification of its gene product in Saccharomyces cerevisiae."
    Kuwahara S., Yoshima T., Fong J., Ogawa T.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Analysis of a 32.8 kb segment of yeast chromosome IV reveals 21 open reading frames, including TPS2, PPH3, RAD55, SED1, PDC2, AFR1, SSS1, SLU7 and a tRNA for arginine."
    Coster F., Jonniaux J.-L., Goffeau A.
    Yeast 11:673-679(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAD55_YEAST
AccessioniPrimary (citable) accession number: P38953
Secondary accession number(s): D6VS62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1050 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.