P38947 (SUCD_CLOK5) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 60.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize orderNames and origin
| Protein names | Recommended name: Succinate-semialdehyde dehydrogenase (acetylating) EC=1.2.1.76 | ||||
| Gene names |
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| Organism | Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 431943 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium |
Protein attributes
| Sequence length | 453 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the reduction of succinate semialdehyde to succinyl-CoA. The enzyme is specific for succinate semialdehyde and succinyl-CoA, and only shows low activity with palmitoyl-CoA. There is no activity with NAD+ as cosubstrate. |
| Catalytic activity | Succinate semialdehyde + coenzyme A + NADP+ = succinyl-CoA + NADPH. |
| Subunit structure | Homodimer. |
| Biophysicochemical properties | Kinetic parameters: KM=4.3 mM for NADPH KM=3.2 mM for succinyl-CoA KM=2.7 mM for succinate semialdehyde KM=2.9 mM for CoA KM=4.0 mM for NADP+ Vmax=28.9 µmol/min/mg enzyme toward NADPH Vmax=28.9 µmol/min/mg enzyme toward succinyl-CoA Vmax=28.3 µmol/min/mg enzyme toward succinate semialdehyde Vmax=27.3 µmol/min/mg enzyme toward CoA Vmax=31.2 µmol/min/mg enzyme toward NADP+ pH dependence: Optimum pH is 7.0 for the reduction reaction and 8.5 for the oxidation reaction. |
Ontologies
| Keywords | |
|---|---|
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Molecular function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 453 | 453 | Succinate-semialdehyde dehydrogenase (acetylating) | PRO_0000072297 | |||||
Regions | |||||||||
| Nucleotide binding | 188 – 193 | 6 | NADP By similarity | ||||||
Sites | |||||||||
| Active site | 242 | 1 | By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 16 | 1 | V → A in AAA92347. Ref.1 | ||||||
| Sequence conflict | 379 | 1 | A → ARTVLPISRLVVNQPATTAG in AAA92347. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular analysis of the anaerobic succinate degradation pathway in Clostridium kluyveri." Soehling B., Gottschalk G. J. Bacteriol. 178:871-880(1996) [PubMed: 8550525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-24. |
| [2] | "The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features." Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed: 18218779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8527 / DSM 555 / NCIMB 10680. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | L21902 Genomic DNA. Translation: AAA92347.1. CP000673 Genomic DNA. Translation: EDK35023.1. |
| RefSeq | YP_001396394.1. NC_009706.1. |
3D structure databases | |
| ProteinModelPortal | P38947. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P38947. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 5394466. |
| GenomeReviews | Gene locus CKL_3015 in contig CP000673_GR. |
| KEGG | ckl:CKL_3015. |
| PATRIC | 19467578. VBICloKlu111549_3119. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG1012. |
| HOGENOM | HBG373091. |
| OMA | SLSMGCG. |
| ProtClustDB | CLSK922707. |
Enzyme and pathway databases | |
| BioCyc | CKLU431943:CKL_3015-MONOMER. MetaCyc:MONOMER-13462. |
Family and domain databases | |
| InterPro | IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 2 hits. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit. |
| Pfam | PF00171. Aldedh. 1 hit. [Graphical view] |
| SUPFAM | SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | SUCD_CLOK5 | ||||||||
| Accession | Primary (citable) accession number: P38947 Secondary accession number(s): A5N1M7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||

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