P38945 (4HDB_CLOK5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD-dependent 4-hydroxybutyrate dehydrogenase
Short name=4HBD
EC=1.1.1.61

Gene names

Name:	4hbD
Ordered Locus Names:	CKL_3014

Organism

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) [Complete proteome] [HAMAP]

Taxonomic identifier

431943 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium kluyveri

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	4-hydroxybutanoate + NAD+ = succinate semialdehyde + NADH.
Cofactor	Divalent cations.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	4-hydroxybutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	NAD-dependent 4-hydroxybutyrate dehydrogenase		PRO_0000087840

Sequences

	Sequence		Length	Mass (Da)
	P38945 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 8D4A8DCD94E252C4	FASTA	371	41,756
	10 20 30 40 50 60 MKLLKLAPDV YKFDTAEEFM KYFKVGKGDF ILTNEFLYKP FLEKFNDGAD AVFQEKYGLG 70 80 90 100 110 120 EPSDEMINNI IKDIGDKQYN RIIAVGGGSV IDIAKILSLK YTDDSLDLFE GKVPLVKNKE 130 140 150 160 170 180 LIIVPTTCGT GSEVTNVSVA ELKRRHTKKG IASDELYATY AVLVPEFIKG LPYKFFVTSS 190 200 210 220 230 240 VDALIHATEA YVSPNANPYT DMFSVKAMEL ILNGYMQMVE KGNDYRVEII EDFVIGSNYA 250 260 270 280 290 300 GIAFGNAGVG AVHALSYPIG GNYHVPHGEA NYLFFTEIFK TYYEKNPNGK IKDVNKLLAG 310 320 330 340 350 360 ILKCDESEAY DSLSQLLDKL LSRKPLREYG MKEEEIETFA DSVIEGQQRL LVNNYEPFSR 370 EDIVNTYKKL Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of the anaerobic succinate degradation pathway in Clostridium kluyveri." Soehling B., Gottschalk G. J. Bacteriol. 178:871-880(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features." Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H., Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F., Hagemeier C., Thauer R.K., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8527 / DSM 555 / NCIMB 10680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L21902 Genomic DNA. Translation: AAA92348.1. CP000673 Genomic DNA. Translation: EDK35022.1.
RefSeq	YP_001396393.1. NC_009706.1.
3D structure databases
ProteinModelPortal	P38945.
ModBase	Search...
Protein-protein interaction databases
STRING	431943.CKL_3014.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EDK35022; EDK35022; CKL_3014.
GeneID	5394465.
KEGG	ckl:CKL_3014.
PATRIC	19467576. VBICloKlu111549_3118.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1454.
HOGENOM	HOG000233530.
OMA	GKYHVPH.
ProtClustDB	CLSK882855.
Enzyme and pathway databases
BioCyc	CKLU431943:GJF1-3007-MONOMER. MetaCyc:MONOMER-13466.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4HDB_CLOK5

Accession

Primary (citable) accession number: P38945
Secondary accession number(s): A5N1M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program