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P38936

- CDN1A_HUMAN

UniProt

P38936 - CDN1A_HUMAN

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Protein

Cyclin-dependent kinase inhibitor 1

Gene

CDKN1A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri13 – 4129C4-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. cyclin-dependent protein kinase activating kinase activity Source: UniProtKB
  2. cyclin-dependent protein serine/threonine kinase inhibitor activity Source: BHF-UCL
  3. metal ion binding Source: UniProtKB-KW
  4. ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  1. cell cycle arrest Source: BHF-UCL
  2. cellular response to DNA damage stimulus Source: BHF-UCL
  3. cellular response to extracellular stimulus Source: BHF-UCL
  4. cellular response to ionizing radiation Source: BHF-UCL
  5. cellular senescence Source: BHF-UCL
  6. DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: BHF-UCL
  7. epidermal growth factor receptor signaling pathway Source: Reactome
  8. Fc-epsilon receptor signaling pathway Source: Reactome
  9. fibroblast growth factor receptor signaling pathway Source: Reactome
  10. G1/S transition of mitotic cell cycle Source: BHF-UCL
  11. G2/M transition of mitotic cell cycle Source: BHF-UCL
  12. innate immune response Source: Reactome
  13. intrinsic apoptotic signaling pathway Source: ProtInc
  14. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
  15. mitotic cell cycle Source: Reactome
  16. mitotic cell cycle arrest Source: Ensembl
  17. negative regulation of apoptotic process Source: Ensembl
  18. negative regulation of cell growth Source: BHF-UCL
  19. negative regulation of cell proliferation Source: BHF-UCL
  20. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl
  21. negative regulation of gene expression Source: Ensembl
  22. negative regulation of phosphorylation Source: BHF-UCL
  23. neurotrophin TRK receptor signaling pathway Source: Reactome
  24. organ regeneration Source: Ensembl
  25. phosphatidylinositol-mediated signaling Source: Reactome
  26. positive regulation of B cell proliferation Source: Ensembl
  27. positive regulation of fibroblast proliferation Source: BHF-UCL
  28. positive regulation of programmed cell death Source: Ensembl
  29. positive regulation of protein kinase activity Source: GOC
  30. positive regulation of reactive oxygen species metabolic process Source: BHF-UCL
  31. protein phosphorylation Source: GOC
  32. Ras protein signal transduction Source: BHF-UCL
  33. regulation of cyclin-dependent protein serine/threonine kinase activity Source: ProtInc
  34. regulation of DNA biosynthetic process Source: Ensembl
  35. regulation of protein import into nucleus, translocation Source: Ensembl
  36. response to arsenic-containing substance Source: Ensembl
  37. response to corticosterone Source: Ensembl
  38. response to drug Source: Ensembl
  39. response to hyperoxia Source: Ensembl
  40. response to organonitrogen compound Source: Ensembl
  41. response to toxic substance Source: Ensembl
  42. response to UV Source: Ensembl
  43. stress-induced premature senescence Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Protein kinase inhibitor

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_1156. Orc1 removal from chromatin.
REACT_12564. AKT phosphorylates targets in the cytosol.
REACT_147727. Constitutive PI3K/AKT Signaling in Cancer.
REACT_1625. p53-Dependent G1 DNA Damage Response.
REACT_169168. Senescence-Associated Secretory Phenotype (SASP).
REACT_169185. DNA Damage/Telomere Stress Induced Senescence.
REACT_821. Cyclin D associated events in G1.
REACT_9003. SCF(Skp2)-mediated degradation of p27/p21.
REACT_9029. Cyclin A:Cdk2-associated events at S phase entry.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase inhibitor 1
Alternative name(s):
CDK-interacting protein 1
Melanoma differentiation-associated protein 6
Short name:
MDA-6
p21
Gene namesi
Name:CDKN1A
Synonyms:CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:1784. CDKN1A.

Subcellular locationi

GO - Cellular componenti

  1. cyclin-dependent protein kinase holoenzyme complex Source: BHF-UCL
  2. cytosol Source: Reactome
  3. intracellular membrane-bounded organelle Source: HPA
  4. nucleoplasm Source: Reactome
  5. nucleus Source: BHF-UCL
  6. PCNA-p21 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi114 – 1141S → E: Phosphomimetic mutant, increases ubiquitination by the DCX(DTL) complex. 1 Publication
Mutagenesisi144 – 1507QTSMTDF → ATSATDA: Abolishes interaction with PCNA and subsequent degradation by the proteasome. 1 Publication
Mutagenesisi145 – 1451T → A: Reduces phosphorylation by Akt; no change in interaction with PCNA, CDK2 or CDK4; no change in subcellular location. 1 Publication
Mutagenesisi145 – 1451T → D: No interaction with PCNA; 59% inhibition of CDK2 binding; modest inhibition of CDK4 binding; no change in subcellular location. 1 Publication
Mutagenesisi146 – 1461S → A: No change in interaction with PCNA. 1 Publication
Mutagenesisi146 – 1461S → D: Reduces interaction with PCNA. 1 Publication
Mutagenesisi147 – 1515MTDFY → ATDAAA: Abolishes interaction with PCNA and subsequent degradation by the proteasome. 1 Publication
Mutagenesisi154 – 1563KRR → AAA: Abolishes degradation by the proteasome without affecting the interaction with PCNA. 1 Publication

Organism-specific databases

Orphaneti652. Multiple endocrine neoplasia type 1.
PharmGKBiPA104.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 164163Cyclin-dependent kinase inhibitor 1PRO_0000190079Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Cross-linki2 – 2Glycyl serine ester (Ser-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei114 – 1141Phosphoserine; by GSK3-beta1 Publication
Modified residuei130 – 1301Phosphoserine2 Publications
Modified residuei145 – 1451Phosphothreonine; by PKA; PKB/AKT1, PIM1 and PIM25 Publications
Modified residuei146 – 1461Phosphoserine; by PKC1 Publication
Modified residuei160 – 1601Phosphoserine; by PKC; in vitro1 Publication

Post-translational modificationi

Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability.8 Publications
Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114; leading to proteasomal degradation.1 Publication
Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1 By similarity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP38936.
PRIDEiP38936.

2D gel databases

SWISS-2DPAGEP38936.

PTM databases

PhosphoSiteiP38936.

Expressioni

Tissue specificityi

Expressed in all adult tissues, with 5-fold lower levels observed in the brain.

Inductioni

Activated by p53/TP53, mezerein (antileukemic compound) and IFNB1. Repressed by HDAC1.2 Publications

Gene expression databases

BgeeiP38936.
CleanExiHS_CDKN1A.
ExpressionAtlasiP38936. baseline and differential.
GenevestigatoriP38936.

Organism-specific databases

HPAiCAB000064.
HPA051359.

Interactioni

Subunit structurei

Interacts with HDAC1; the interaction is prevented by competitive binding of C10orf90/FATS to HDAC1 facilitating acetylation and protein stabilization of CDKN1A/p21 By similarity. Interacts with MKRN1. Interacts with PSMA3. Interacts with PCNA. Component of the ternary complex, cyclin D-CDK4-CDKN1A. Interacts (via its N-terminal domain) with CDK4; the interaction promotes the assembly of the cyclin D-CDK4 complex, its nuclear translocation and promotes the cyclin D-dependent enzyme activity of CDK4. Binding to CDK2 leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Interacts with PIM1.By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P279583EBI-375077,EBI-6377335From a different organism.
CCNA1P783963EBI-375077,EBI-375065
CCNA2P202482EBI-375077,EBI-457097
CCND1P243856EBI-375077,EBI-375001
CCND2P302793EBI-375077,EBI-748789
CCND3P302814EBI-375077,EBI-375013
CCNE1P248649EBI-375077,EBI-519526
CCNE2O960202EBI-375077,EBI-375033
CDC45O754192EBI-375077,EBI-374969
CDC6Q997412EBI-375077,EBI-374862
CDK14O949218EBI-375077,EBI-1043945
CDK2P2494114EBI-375077,EBI-375096
CDK4P118025EBI-375077,EBI-295644
CDK5Q005354EBI-375077,EBI-1041567
MKRN1Q9UHC75EBI-375077,EBI-373524
NABP2Q9BQ157EBI-375077,EBI-2120336
PCNAP120046EBI-375077,EBI-358311
PCNAQ6FI352EBI-375077,EBI-8469539
SIPA1Q96FS42EBI-375077,EBI-1054981
SKP1P632083EBI-375077,EBI-307486
SKP2Q133092EBI-375077,EBI-456291
TP53P046373EBI-375077,EBI-366083

Protein-protein interaction databases

BioGridi107460. 251 interactions.
DIPiDIP-246N.
IntActiP38936. 152 interactions.
MINTiMINT-104203.
STRINGi9606.ENSP00000244741.

Structurei

Secondary structure

1
164
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi147 – 1493
Beta strandi151 – 1588

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AXCX-ray2.60B/D/F139-160[»]
2ZVVX-ray2.00X/Y139-160[»]
2ZVWX-ray2.50I/J/K/L/M/N/O/P139-160[»]
DisProtiDP00016.
ProteinModelPortaliP38936.
SMRiP38936. Positions 17-77.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38936.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 248Required for binding cyclins
Regioni53 – 586Required for binding CDKs

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi140 – 16425PIP-box K+4 motifAdd
BLAST
Motifi141 – 15616Nuclear localization signalSequence AnalysisAdd
BLAST

Domaini

The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.

Sequence similaritiesi

Belongs to the CDI family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri13 – 4129C4-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG290902.
GeneTreeiENSGT00530000063588.
HOGENOMiHOG000285999.
HOVERGENiHBG050868.
InParanoidiP38936.
KOiK06625.
OrthoDBiEOG7GJ6HD.
PhylomeDBiP38936.
TreeFamiTF101038.

Family and domain databases

InterProiIPR003175. CDI.
[Graphical view]
PANTHERiPTHR10265. PTHR10265. 1 hit.
PfamiPF02234. CDI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38936-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEPAGDVRQ NPCGSKACRR LFGPVDSEQL SRDCDALMAG CIQEARERWN
60 70 80 90 100
FDFVTETPLE GDFAWERVRG LGLPKLYLPT GPRRGRDELG GGRRPGTSPA
110 120 130 140 150
LLQGTAEEDH VDLSLSCTLV PRSGEQAEGS PGGPGDSQGR KRRQTSMTDF
160
YHSKRRLIFS KRKP
Length:164
Mass (Da):18,119
Last modified:January 23, 2007 - v3
Checksum:i98D1E7C519ADFCA9
GO

Sequence cautioni

The sequence AAB59559.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAB59560.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti4 – 41P → L.
Corresponds to variant rs4986866 [ dbSNP | Ensembl ].
VAR_048686
Natural varianti31 – 311S → R.3 Publications
Corresponds to variant rs1801270 [ dbSNP | Ensembl ].
VAR_011870
Natural varianti63 – 631F → L.
Corresponds to variant rs4986867 [ dbSNP | Ensembl ].
VAR_048687
Natural varianti149 – 1491D → G.
Corresponds to variant rs1801724 [ dbSNP | Ensembl ].
VAR_014875

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25610 mRNA. Translation: AAA16109.1.
S67388 mRNA. Translation: AAB29246.1.
U09579 mRNA. Translation: AAA85641.1.
U03106 mRNA. Translation: AAC04313.1.
L26165 mRNA. Translation: AAA19811.1.
L47232 mRNA. Translation: AAB59559.1. Different initiation.
L47233 mRNA. Translation: AAB59560.1. Different initiation.
AF497972 Genomic DNA. Translation: AAM11787.1.
BT006719 mRNA. Translation: AAP35365.1.
AB451290 mRNA. Translation: BAG70104.1.
AB451422 mRNA. Translation: BAG70236.1.
CR536533 mRNA. Translation: CAG38770.1.
Z85996 Genomic DNA. Translation: CAB06656.1.
CH471081 Genomic DNA. Translation: EAX03904.1.
BC000275 mRNA. Translation: AAH00275.1.
BC000312 mRNA. Translation: AAH00312.1.
BC001935 mRNA. Translation: AAH01935.1.
BC013967 mRNA. Translation: AAH13967.1.
CCDSiCCDS4824.1.
PIRiI54380.
I68674.
RefSeqiNP_000380.1. NM_000389.4.
NP_001207706.1. NM_001220777.1.
NP_001207707.1. NM_001220778.1.
NP_001278478.1. NM_001291549.1.
NP_510867.1. NM_078467.2.
UniGeneiHs.370771.
Hs.732576.

Genome annotation databases

EnsembliENST00000244741; ENSP00000244741; ENSG00000124762.
ENST00000373711; ENSP00000362815; ENSG00000124762.
ENST00000405375; ENSP00000384849; ENSG00000124762.
ENST00000448526; ENSP00000409259; ENSG00000124762.
ENST00000615513; ENSP00000482768; ENSG00000124762.
GeneIDi1026.
KEGGihsa:1026.
UCSCiuc003omm.4. human.

Polymorphism databases

DMDMi729143.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25610 mRNA. Translation: AAA16109.1 .
S67388 mRNA. Translation: AAB29246.1 .
U09579 mRNA. Translation: AAA85641.1 .
U03106 mRNA. Translation: AAC04313.1 .
L26165 mRNA. Translation: AAA19811.1 .
L47232 mRNA. Translation: AAB59559.1 . Different initiation.
L47233 mRNA. Translation: AAB59560.1 . Different initiation.
AF497972 Genomic DNA. Translation: AAM11787.1 .
BT006719 mRNA. Translation: AAP35365.1 .
AB451290 mRNA. Translation: BAG70104.1 .
AB451422 mRNA. Translation: BAG70236.1 .
CR536533 mRNA. Translation: CAG38770.1 .
Z85996 Genomic DNA. Translation: CAB06656.1 .
CH471081 Genomic DNA. Translation: EAX03904.1 .
BC000275 mRNA. Translation: AAH00275.1 .
BC000312 mRNA. Translation: AAH00312.1 .
BC001935 mRNA. Translation: AAH01935.1 .
BC013967 mRNA. Translation: AAH13967.1 .
CCDSi CCDS4824.1.
PIRi I54380.
I68674.
RefSeqi NP_000380.1. NM_000389.4.
NP_001207706.1. NM_001220777.1.
NP_001207707.1. NM_001220778.1.
NP_001278478.1. NM_001291549.1.
NP_510867.1. NM_078467.2.
UniGenei Hs.370771.
Hs.732576.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AXC X-ray 2.60 B/D/F 139-160 [» ]
2ZVV X-ray 2.00 X/Y 139-160 [» ]
2ZVW X-ray 2.50 I/J/K/L/M/N/O/P 139-160 [» ]
DisProti DP00016.
ProteinModelPortali P38936.
SMRi P38936. Positions 17-77.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107460. 251 interactions.
DIPi DIP-246N.
IntActi P38936. 152 interactions.
MINTi MINT-104203.
STRINGi 9606.ENSP00000244741.

Chemistry

ChEMBLi CHEMBL5021.

PTM databases

PhosphoSitei P38936.

Polymorphism databases

DMDMi 729143.

2D gel databases

SWISS-2DPAGE P38936.

Proteomic databases

PaxDbi P38936.
PRIDEi P38936.

Protocols and materials databases

DNASUi 1026.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000244741 ; ENSP00000244741 ; ENSG00000124762 .
ENST00000373711 ; ENSP00000362815 ; ENSG00000124762 .
ENST00000405375 ; ENSP00000384849 ; ENSG00000124762 .
ENST00000448526 ; ENSP00000409259 ; ENSG00000124762 .
ENST00000615513 ; ENSP00000482768 ; ENSG00000124762 .
GeneIDi 1026.
KEGGi hsa:1026.
UCSCi uc003omm.4. human.

Organism-specific databases

CTDi 1026.
GeneCardsi GC06P037308.
HGNCi HGNC:1784. CDKN1A.
HPAi CAB000064.
HPA051359.
MIMi 116899. gene.
neXtProti NX_P38936.
Orphaneti 652. Multiple endocrine neoplasia type 1.
PharmGKBi PA104.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG290902.
GeneTreei ENSGT00530000063588.
HOGENOMi HOG000285999.
HOVERGENi HBG050868.
InParanoidi P38936.
KOi K06625.
OrthoDBi EOG7GJ6HD.
PhylomeDBi P38936.
TreeFami TF101038.

Enzyme and pathway databases

Reactomei REACT_1156. Orc1 removal from chromatin.
REACT_12564. AKT phosphorylates targets in the cytosol.
REACT_147727. Constitutive PI3K/AKT Signaling in Cancer.
REACT_1625. p53-Dependent G1 DNA Damage Response.
REACT_169168. Senescence-Associated Secretory Phenotype (SASP).
REACT_169185. DNA Damage/Telomere Stress Induced Senescence.
REACT_821. Cyclin D associated events in G1.
REACT_9003. SCF(Skp2)-mediated degradation of p27/p21.
REACT_9029. Cyclin A:Cdk2-associated events at S phase entry.

Miscellaneous databases

ChiTaRSi CDKN1A. human.
EvolutionaryTracei P38936.
GeneWikii P21.
GenomeRNAii 1026.
NextBioi 4309.
PROi P38936.
SOURCEi Search...

Gene expression databases

Bgeei P38936.
CleanExi HS_CDKN1A.
ExpressionAtlasi P38936. baseline and differential.
Genevestigatori P38936.

Family and domain databases

InterProi IPR003175. CDI.
[Graphical view ]
PANTHERi PTHR10265. PTHR10265. 1 hit.
Pfami PF02234. CDI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases."
    Harper J.W., Adami G.R., Wei N., Keyomarsi K., Elledge S.J.
    Cell 75:805-816(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Use of a sensitive and efficient subtraction hybridization protocol for the identification of genes differentially regulated during the induction of differentiation in human melanoma cells."
    Jiang H., Fisher P.B.
    Mol. Cell. Differ. 1:285-299(1993)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "The melanoma differentiation-associated gene mda-6, which encodes the cyclin-dependent kinase inhibitor p21, is differentially expressed during growth, differentiation and progression in human melanoma cells."
    Jiang H., Lin J., Su Z.Z., Herlyn M., Kerbel R.S., Weissman B.E., Welch D.R., Fisher P.B.
    Oncogene 10:1855-1864(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  6. "Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen."
    Noda A., Ning Y., Venable S.F., Pereira-Smith O.M., Smith J.R.
    Exp. Cell Res. 211:90-98(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  7. "Two variants of the CIP1/WAF1 gene occur together and are associated with human cancer."
    Mousses S., Oezcelik H., Lee P.D., Malkin D., Bull S.B., Andrulis I.L.
    Hum. Mol. Genet. 4:1089-1092(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-31.
  8. NIEHS SNPs program
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-31.
  9. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  10. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  11. "Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
    Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B.
    , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
    Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  12. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  13. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  14. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-31.
    Tissue: Eye and Lung.
  15. "N-acetylation and ubiquitin-independent proteasomal degradation of p21(Cip1)."
    Chen X., Chi Y., Bloecher A., Aebersold R., Clurman B.E., Roberts J.M.
    Mol. Cell 16:839-847(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
  16. "Reversible phosphorylation at the C-terminal regulatory domain of p21(Waf1/Cip1) modulates proliferating cell nuclear antigen binding."
    Scott M.T., Morrice N., Ball K.L.
    J. Biol. Chem. 275:11529-11537(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 136-148, PHOSPHORYLATION AT THR-145; SER-146 AND SER-160, IDENTIFICATION BY MASS SPECTROMETRY.
  17. Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CDK4 AND CCND1 IN THE CYCLIN D-CDK4-CDKN1A COMPLEX.
  18. "A degradation signal located in the C-terminus of p21WAF1/CIP1 is a binding site for the C8 alpha-subunit of the 20S proteasome."
    Touitou R., Richardson J., Bose S., Nakanishi M., Rivett J., Allday M.J.
    EMBO J. 20:2367-2375(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PSMA3.
  19. "Akt-dependent phosphorylation of p21(Cip1) regulates PCNA binding and proliferation of endothelial cells."
    Roessig L., Jadidi A.S., Urbich C., Badorff C., Zeiher A.M., Dimmeler S.
    Mol. Cell. Biol. 21:5644-5657(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-145, MUTAGENESIS OF THR-145 AND SER-146, SUBCELLULAR LOCATION.
  20. "Phosphorylation of the cell cycle inhibitor p21Cip1/WAF1 by Pim-1 kinase."
    Wang Z., Bhattacharya N., Mixter P.F., Wei W., Sedivy J., Magnuson N.S.
    Biochim. Biophys. Acta 1593:45-55(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-145 BY PIM1, SUBCELLULAR LOCATION, INTERACTION WITH PIM1.
  21. "N-Terminal ubiquitination of extracellular signal-regulated kinase 3 and p21 directs their degradation by the proteasome."
    Coulombe P., Rodier G., Bonneil E., Thibault P., Meloche S.
    Mol. Cell. Biol. 24:6140-6150(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION AT SER-2.
  22. "Only Akt1 is required for proliferation, while Akt2 promotes cell cycle exit through p21 binding."
    Heron-Milhavet L., Franckhauser C., Rana V., Berthenet C., Fisher D., Hemmings B.A., Fernandez A., Lamb N.J.
    Mol. Cell. Biol. 26:8267-8280(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-145.
  23. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  24. "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex."
    Abbas T., Sivaprasad U., Terai K., Amador V., Pagano M., Dutta A.
    Genes Dev. 22:2496-2506(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, DOMAIN PIP-BOX K+4 MOTIF, INTERACTION WITH PCNA, MUTAGENESIS OF SER-114 AND 144-GLN--PHE-150, PHOSPHORYLATION AT SER-114.
  25. "The CRL4Cdt2 ubiquitin ligase targets the degradation of p21Cip1 to control replication licensing."
    Kim Y., Starostina N.G., Kipreos E.T.
    Genes Dev. 22:2507-2519(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION.
  26. "CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation."
    Nishitani H., Shiomi Y., Iida H., Michishita M., Takami T., Tsurimoto T.
    J. Biol. Chem. 283:29045-29052(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, DOMAIN PIP-BOX K+4 MOTIF, MUTAGENESIS OF 147-MET--TYR-151 AND 154-LYS--ARG-156, INTERACTION WITH PCNA.
  27. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  28. "A dual role of Cdk2 in DNA damage response."
    Satyanarayana A., Kaldis P.
    Cell Div. 4:9-9(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON DNA REPAIR, INTERACTION WITH CDK2.
  29. "Differential regulation of p53 and p21 by MKRN1 E3 ligase controls cell cycle arrest and apoptosis."
    Lee E.-W., Lee M.-S., Camus S., Ghim J., Yang M.-R., Oh W., Ha N.-C., Lane D.P., Song J.
    EMBO J. 28:2100-2113(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, INTERACTION WITH MKRN1.
  30. "Ionizing radiation induces ATM-independent degradation of p21Cip1 in transformed cells."
    Stuart S.A., Wang J.Y.
    J. Biol. Chem. 284:15061-15070(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION.
  31. "Pim-2 phosphorylation of p21(Cip1/WAF1) enhances its stability and inhibits cell proliferation in HCT116 cells."
    Wang Z., Zhang Y., Gu J.J., Davitt C., Reeves R., Magnuson N.S.
    Int. J. Biochem. Cell Biol. 42:1030-1038(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-145 BY PIM2.
  32. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  33. "ZNF313 is a novel cell cycle activator with an E3 ligase activity inhibiting cellular senescence by destabilizing p21(WAF1.)."
    Han J., Kim Y.L., Lee K.W., Her N.G., Ha T.K., Yoon S., Jeong S.I., Lee J.H., Kang M.J., Lee M.G., Ryu B.K., Baik J.H., Chi S.G.
    Cell Death Differ. 20:1055-1067(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION BY RNF114.
  34. "Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA."
    Gulbis J.M., Kelman Z., Hurwitz J., O'Donnell M., Kuriyan J.
    Cell 87:297-306(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 139-160 IN COMPLEX WITH PCNA.

Entry informationi

Entry nameiCDN1A_HUMAN
AccessioniPrimary (citable) accession number: P38936
Secondary accession number(s): Q14010, Q6FI05, Q9BUT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 169 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3