Calcium-transporting ATPase 2 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P38929 (ATC2_YEAST)

Last modified February 9, 2010. Version 97. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Calcium-transporting ATPase 2
    EC=3.6.3.8
Alternative name(s):
    Vacuolar Ca(2+)-ATPase

Gene names

Name:	PMC1
Ordered Locus Names:	YGL006W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	1173 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports the calcium to the vacuole and participates in the control of the cytosolic free calcium.
Catalytic activity	ATP + H₂O + Ca²⁺(Cis) = ADP + phosphate + Ca²⁺(Trans).
Subcellular location	Vacuole membrane; Multi-pass membrane protein.
Miscellaneous	Present with 98 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the cation transport ATPase (P-type) family.

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Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane
Ligand	ATP-binding Calcium Magnesium Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro calcium ion transport Ref.1 Inferred from mutant phenotype. Source: SGD cellular calcium ion homeostasis Ref.1 Inferred from mutant phenotype. Source: SGD transmembrane transport Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	fungal-type vacuole membrane Ref.1 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Ref.1 Inferred from mutant phenotype. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-3097,EBI-3097
	P38845	1	EBI-3097,EBI-24770
	Q03730	1	EBI-3097,EBI-27739
	Q06328	1	EBI-3097,EBI-37173
AGP1	P25376	1	EBI-3097,EBI-2357
ATG27	P46989	1	EBI-3097,EBI-26164
COT1	P32798	1	EBI-3097,EBI-5006
CTR2	P38865	1	EBI-3097,EBI-5268
DPP1	Q05521	1	EBI-3097,EBI-2054360
EMP47	P43555	1	EBI-3097,EBI-6439
FET5	P43561	1	EBI-3097,EBI-6891
FMP42	Q04991	1	EBI-3097,EBI-27478
FTH1	P38310	1	EBI-3097,EBI-20959
GAP1	P19145	1	EBI-3097,EBI-7314
GTR2	P53290	1	EBI-3097,EBI-7962
HXT1	P32465	1	EBI-3097,EBI-8759
MBF1	O14467	1	EBI-3097,EBI-2043025
MEH1	Q02205	1	EBI-3097,EBI-27062
MRL1	Q06815	1	EBI-3097,EBI-33784
PIB2	P53191	1	EBI-3097,EBI-13400
PMP2	P40975	1	EBI-3097,EBI-2043041
PMP3	P87284	1	EBI-3097,EBI-13555
SFT2	P38166	1	EBI-3097,EBI-17046
SNA4	Q07549	1	EBI-3097,EBI-22078
TMS1	Q12116	1	EBI-3097,EBI-19306
VAC8	P39968	1	EBI-3097,EBI-20212
VBA4	Q04602	1	EBI-3097,EBI-2045815
VMA13	P41807	1	EBI-3097,EBI-20281
VMA7	P39111	1	EBI-3097,EBI-20272
VMA8	P32610	1	EBI-3097,EBI-20264
VPH1	P32563	1	EBI-3097,EBI-20455
VPS35	P34110	1	EBI-3097,EBI-20415
VPS68	Q12016	1	EBI-3097,EBI-29309
YBT1	P32386	1	EBI-3097,EBI-21511

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1173

1173

Calcium-transporting ATPase 2

PRO_0000046232

Regions

Topological domain

1 – 114

114

Cytoplasmic Potential

Transmembrane

115 – 139

Potential

Topological domain

140 – 152

Extracellular Potential

Transmembrane

153 – 173

Potential

Topological domain

174 – 349

176

Cytoplasmic Potential

Transmembrane

350 – 368

Potential

Topological domain

369 – 388

Extracellular Potential

Transmembrane

389 – 409

Potential

Topological domain

410 – 899

490

Cytoplasmic Potential

Transmembrane

900 – 922

Potential

Topological domain

923 – 929

Extracellular Potential

Transmembrane

930 – 950

Potential

Topological domain

951 – 976

Cytoplasmic Potential

Transmembrane

977 – 998

Potential

Topological domain

999 – 1010

Extracellular Potential

Transmembrane

1011 – 1029

Potential

Topological domain

1030 – 1065

Cytoplasmic Potential

Transmembrane

1066 – 1086

Potential

Topological domain

1087 – 1099

Extracellular Potential

Transmembrane

1100 – 1120

Potential

Topological domain

1121 – 1173

Cytoplasmic Potential

Sites

Active site

445

4-aspartylphosphate intermediate By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P38929-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 5BD9ECFF8508F396
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FASTA

1,173

130,861

        10         20         30         40         50         60 
MSRQDENSAL LANNENNKPS YTGNENGVYD NFKLSKSQLS DLHNPKSIRS FVRLFGYESN 

        70         80         90        100        110        120 
SLFKYLKTDK NAGISLPEIS NYRKTNRYKN YGDNSLPERI PKSFLQLVWA AFNDKTMQLL 

       130        140        150        160        170        180 
TVAAVVSFVL GLYELWMQPP QYDPEGNKIK QVDWIEGVAI MIAVFVVVLV SAANDYQKEL 

       190        200        210        220        230        240 
QFAKLNKKKE NRKIIVIRND QEILISIHHV LVGDVISLQT GDVVPADCVM ISGKCEADES 

       250        260        270        280        290        300 
SITGESNTIQ KFPVDNSLRD FKKFNSIDSH NHSKPLDIGD VNEDGNKIAD CMLISGSRIL 

       310        320        330        340        350        360 
SGLGRGVITS VGINSVYGQT MTSLNAEPES TPLQLHLSQL ADNISVYGCV SAIILFLVLF 

       370        380        390        400        410        420 
TRYLFYIIPE DGRFHDLDPA QKGSKFMNIF ITSITVIVVA VPEGLPLAVT LALAFATTRM 

       430        440        450        460        470        480 
TKDGNLVRVL RSCETMGSAT AVCSDKTGTL TENVMTVVRG FPGNSKFDDS KSLPVSEQRK 

       490        500        510        520        530        540 
LNSKKVFEEN CSSSLRNDLL ANIVLNSTAF ENRDYKKNDK NTNGSKNMSK NLSFLDKCKS 

       550        560        570        580        590        600 
RLSFFKKGNR EDDEDQLFKN VNKGRQEPFI GSKTETALLS LARLSLGLQP GELQYLRDQP 

       610        620        630        640        650        660 
MEKFNIEKVV QTIPFESSRK WAGLVVKYKE GKNKKPFYRF FIKGAAEIVS KNCSYKRNSD 

       670        680        690        700        710        720 
DTLEEINEDN KKETDDEIKN LASDALRAIS VAHKDFCECD SWPPEQLRDK DSPNIAALDL 

       730        740        750        760        770        780 
LFNSQKGLIL DGLLGIQDPL RAGVRESVQQ CQRAGVTVRM VTGDNILTAK AIARNCAILS 

       790        800        810        820        830        840 
TDISSEAYSA MEGTEFRKLT KNERIRILPN LRVLARSSPE DKRLLVETLK GMGDVVAVTG 

       850        860        870        880        890        900 
DGTNDAPALK LADVGFSMGI SGTEVAREAS DIILMTDDFS AIVNAIKWGR CVSVSIKKFI 

       910        920        930        940        950        960 
QFQLIVNITA VILTFVSSVA SSDETSVLTA VQLLWINLIM DTLAALALAT DKPDPNIMDR 

       970        980        990       1000       1010       1020 
KPRGRSTSLI SVSTWKMILS QATLQLIVTF ILHFYGPELF FKKHEDEITS HQQQQLNAMT 

      1030       1040       1050       1060       1070       1080 
FNTFVWLQFF TMLVSRKLDE GDGISNWRGR ISAANLNFFQ DLGRNYYFLT IMAIIGSCQV 

      1090       1100       1110       1120       1130       1140 
LIMFFGGAPF SIARQTKSMW ITAVLCGMLS LIMGVLVRIC PDEVAVKVFP AAFVQRFKYV 

      1150       1160       1170 
FGLEFLRKNH TGKHDDEEAL LEESDSPEST AFY

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Calcineurin-dependent growth control in Saccharomyces cerevisiae mutants lacking PMC1, a homolog of plasma membrane Ca2+ ATPases." Cunningham K.W., Fink G.R. J. Cell Biol. 124:351-363(1994) [PubMed: 7507493] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	U03060 Unassigned DNA. Translation: AAC48919.1. Z72528 Genomic DNA. Translation: CAA96706.1.
PIR	S48877.
RefSeq	NP_011509.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5901N.
IntAct	P38929. 43 interactions.
STRING	P38929.
Protein family/group databases
TCDB	3.A.3.2.2. P-type ATPase (P-ATPase) superfamily.
Proteomic databases
PeptideAtlas	P38929.
Genome annotation databases
Ensembl	YGL006W; YGL006W; YGL006W; Saccharomyces cerevisiae. [Genome view]
GeneID	852878.
KEGG	sce:YGL006W.
NMPDR	fig\|4932.3.peg.2619.
Organism-specific databases
CYGD	YGL006w.
SGD	S000002974. PMC1.
Phylogenomic databases
eggNOG	fuNOG06319.
HOGENOM	HBG456486.
OMA	EADESSI.
OrthoDB	EOG9M3BS4.
PhylomeDB	P38929.
Enzyme and pathway databases
BRENDA	3.6.3.8. 250.
Gene expression databases
ArrayExpress	P38929.
Genevestigator	P38929.
GermOnline	YGL006W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR006408. ATPase_P-typ_Ca-transp_PMCA. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972517.

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Entry information

Entry name

ATC2_YEAST

Accession

Primary (citable) accession number: P38929

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	February 9, 2010
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents