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P38925

- SMF1_YEAST

UniProt

P38925 - SMF1_YEAST

Protein

Manganese transporter SMF1

Gene

SMF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    High-affinity manganese transporter involved in manganese uptake from the extracellular environment. Contributes also to cellular accumulation of other divalent metal ions such as cadmium, cobalt, copper, iron and nickel.2 Publications

    GO - Molecular functioni

    1. inorganic cation transmembrane transporter activity Source: SGD
    2. metal ion binding Source: UniProtKB-KW
    3. solute:proton symporter activity Source: SGD

    GO - Biological processi

    1. cadmium ion transport Source: SGD
    2. cellular cadmium ion homeostasis Source: SGD
    3. cellular copper ion homeostasis Source: SGD
    4. cellular manganese ion homeostasis Source: SGD
    5. copper ion transport Source: SGD
    6. iron ion transport Source: SGD
    7. manganese ion transport Source: SGD
    8. proton transport Source: GOC

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33518-MONOMER.

    Protein family/group databases

    TCDBi2.A.55.1.1. the metal ion (mn(2+)-iron) transporter (nramp) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Manganese transporter SMF1
    Gene namesi
    Name:SMF1
    Synonyms:ESP1
    Ordered Locus Names:YOL122C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL122c.
    SGDiS000005482. SMF1.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Targeted to the vacuolar lumen in presence of excess manganese, where it is degraded.

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 575575Manganese transporter SMF1PRO_0000212605Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei24 – 241Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38925.
    PaxDbiP38925.

    Expressioni

    Gene expression databases

    GenevestigatoriP38925.

    Interactioni

    Protein-protein interaction databases

    BioGridi34279. 31 interactions.
    IntActiP38925. 3 interactions.
    STRINGi4932.YOL122C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38925.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7070ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini92 – 10817CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini130 – 15627ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini178 – 1792CytoplasmicSequence Analysis
    Topological domaini201 – 21818ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini240 – 26627CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini288 – 34457ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini366 – 39631CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini418 – 46346ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini485 – 54359CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini565 – 57511ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei71 – 9121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei109 – 12921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei157 – 17721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei180 – 20021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei219 – 23921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei267 – 28721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei345 – 36521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei397 – 41721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei464 – 48421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei544 – 56421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NRAMP family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1914.
    GeneTreeiENSGT00390000006526.
    HOGENOMiHOG000152204.
    KOiK12346.
    OMAiLMRGWRV.
    OrthoDBiEOG7NSBBM.

    Family and domain databases

    HAMAPiMF_00221. NRAMP.
    InterProiIPR001046. NRAMP_fam.
    [Graphical view]
    PANTHERiPTHR11706. PTHR11706. 1 hit.
    PfamiPF01566. Nramp. 1 hit.
    [Graphical view]
    PRINTSiPR00447. NATRESASSCMP.
    TIGRFAMsiTIGR01197. nramp. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38925-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNVGPSHAA VAVDASEARK RNISEEVFEL RDKKDSTVVI EGEAPVRTFT    50
    SSSSNHERED TYVSKRQVMR DIFAKYLKFI GPGLMVSVAY IDPGNYSTAV 100
    DAGASNQFSL LCIILLSNFI AIFLQCLCIK LGSVTGLDLS RACREYLPRW 150
    LNWTLYFFAE CAVIATDIAE VIGTAIALNI LIKVPLPAGV AITVVDVFLI 200
    MFTYKPGASS IRFIRIFECF VAVLVVGVCI CFAIELAYIP KSTSVKQVFR 250
    GFVPSAQMFD HNGIYTAISI LGATVMPHSL FLGSALVQPR LLDYDVKHGN 300
    YTVSEEQDKV KKSKSTEEIM EEKYFNYRPT NAAIKYCMKY SMVELSITLF 350
    TLALFVNCAI LVVAGSTLYN SPEADGADLF TIHELLSRNL APAAGTIFML 400
    ALLLSGQSAG VVCTMSGQIV SEGHINWKLQ PWQRRLATRC ISIIPCLVIS 450
    ICIGREALSK ALNASQVVLS IVLPFLVAPL IFFTCKKSIM KTEITVDHTE 500
    EDSHNHQNNN DRSAGSVIEQ DGSSGMEIEN GKDVKIVYMA NNWIITVIAI 550
    IVWLFLSLLN VYAIVQLGMS HGDIS 575
    Length:575
    Mass (Da):63,264
    Last modified:November 1, 1997 - v2
    Checksum:iAC0EEEF1BBF34ACB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti305 – 3051E → D(PubMed:1447206)Curated
    Sequence conflicti416 – 4161S → A(PubMed:1447206)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15929 Genomic DNA. Translation: AAB48984.1.
    X95258 Genomic DNA. Translation: CAA64547.1.
    Z74864 Genomic DNA. Translation: CAA99141.1.
    BK006948 Genomic DNA. Translation: DAA10662.1.
    PIRiS58647.
    RefSeqiNP_014519.1. NM_001183376.1.

    Genome annotation databases

    EnsemblFungiiYOL122C; YOL122C; YOL122C.
    GeneIDi854027.
    KEGGisce:YOL122C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15929 Genomic DNA. Translation: AAB48984.1 .
    X95258 Genomic DNA. Translation: CAA64547.1 .
    Z74864 Genomic DNA. Translation: CAA99141.1 .
    BK006948 Genomic DNA. Translation: DAA10662.1 .
    PIRi S58647.
    RefSeqi NP_014519.1. NM_001183376.1.

    3D structure databases

    ProteinModelPortali P38925.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34279. 31 interactions.
    IntActi P38925. 3 interactions.
    STRINGi 4932.YOL122C.

    Protein family/group databases

    TCDBi 2.A.55.1.1. the metal ion (mn(2+)-iron) transporter (nramp) family.

    Proteomic databases

    MaxQBi P38925.
    PaxDbi P38925.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL122C ; YOL122C ; YOL122C .
    GeneIDi 854027.
    KEGGi sce:YOL122C.

    Organism-specific databases

    CYGDi YOL122c.
    SGDi S000005482. SMF1.

    Phylogenomic databases

    eggNOGi COG1914.
    GeneTreei ENSGT00390000006526.
    HOGENOMi HOG000152204.
    KOi K12346.
    OMAi LMRGWRV.
    OrthoDBi EOG7NSBBM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33518-MONOMER.

    Miscellaneous databases

    NextBioi 975571.

    Gene expression databases

    Genevestigatori P38925.

    Family and domain databases

    HAMAPi MF_00221. NRAMP.
    InterProi IPR001046. NRAMP_fam.
    [Graphical view ]
    PANTHERi PTHR11706. PTHR11706. 1 hit.
    Pfami PF01566. Nramp. 1 hit.
    [Graphical view ]
    PRINTSi PR00447. NATRESASSCMP.
    TIGRFAMsi TIGR01197. nramp. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Two related genes encoding extremely hydrophobic proteins suppress a lethal mutation in the yeast mitochondrial processing enhancing protein."
      West A.H., Clark D.J., Martin J., Neupert W., Hartl F.-U., Horwich A.L.
      J. Biol. Chem. 267:24625-24633(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A yeast manganese transporter related to the macrophage protein involved in conferring resistance to mycobacteria."
      Supek F., Supekova L., Nelson H., Nelson N.
      Proc. Natl. Acad. Sci. U.S.A. 93:5105-5110(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 200060 / W303.
    3. "DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames."
      Lafuente M.J., Gamo F.-J., Gancedo C.
      Yeast 12:1041-1045(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "The family of SMF metal ion transporters in yeast cells."
      Cohen A., Nelson H., Nelson N.
      J. Biol. Chem. 275:33388-33394(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Saccharomyces cerevisiae expresses three functionally distinct homologues of the nramp family of metal transporters."
      Portnoy M.E., Liu X.F., Culotta V.C.
      Mol. Cell. Biol. 20:7893-7902(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    9. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSMF1_YEAST
    AccessioniPrimary (citable) accession number: P38925
    Secondary accession number(s): D6W1U6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3