Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Manganese transporter SMF1

Gene

SMF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity manganese transporter involved in manganese uptake from the extracellular environment. Contributes also to cellular accumulation of other divalent metal ions such as cadmium, cobalt, copper, iron and nickel.2 Publications

GO - Molecular functioni

  • inorganic cation transmembrane transporter activity Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • solute:proton symporter activity Source: SGD

GO - Biological processi

  • cadmium ion transport Source: SGD
  • cellular cadmium ion homeostasis Source: SGD
  • cellular copper ion homeostasis Source: SGD
  • cellular manganese ion homeostasis Source: SGD
  • copper ion transport Source: SGD
  • iron ion transport Source: SGD
  • manganese ion transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33518-MONOMER.
ReactomeiR-SCE-1222556. ROS, RNS production in response to bacteria.
R-SCE-425410. Metal ion SLC transporters.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-6803544. Ion influx/efflux at host-pathogen interface.
R-SCE-917937. Iron uptake and transport.

Protein family/group databases

TCDBi2.A.55.1.1. the metal ion (mn(2+)-iron) transporter (nramp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese transporter SMF1
Gene namesi
Name:SMF1
Synonyms:ESP1
Ordered Locus Names:YOL122C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL122C.
SGDiS000005482. SMF1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 70ExtracellularSequence analysisAdd BLAST70
Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
Topological domaini92 – 108CytoplasmicSequence analysisAdd BLAST17
Transmembranei109 – 129HelicalSequence analysisAdd BLAST21
Topological domaini130 – 156ExtracellularSequence analysisAdd BLAST27
Transmembranei157 – 177HelicalSequence analysisAdd BLAST21
Topological domaini178 – 179CytoplasmicSequence analysis2
Transmembranei180 – 200HelicalSequence analysisAdd BLAST21
Topological domaini201 – 218ExtracellularSequence analysisAdd BLAST18
Transmembranei219 – 239HelicalSequence analysisAdd BLAST21
Topological domaini240 – 266CytoplasmicSequence analysisAdd BLAST27
Transmembranei267 – 287HelicalSequence analysisAdd BLAST21
Topological domaini288 – 344ExtracellularSequence analysisAdd BLAST57
Transmembranei345 – 365HelicalSequence analysisAdd BLAST21
Topological domaini366 – 396CytoplasmicSequence analysisAdd BLAST31
Transmembranei397 – 417HelicalSequence analysisAdd BLAST21
Topological domaini418 – 463ExtracellularSequence analysisAdd BLAST46
Transmembranei464 – 484HelicalSequence analysisAdd BLAST21
Topological domaini485 – 543CytoplasmicSequence analysisAdd BLAST59
Transmembranei544 – 564HelicalSequence analysisAdd BLAST21
Topological domaini565 – 575ExtracellularSequence analysisAdd BLAST11

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002126051 – 575Manganese transporter SMF1Add BLAST575

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineCombined sources1
Cross-linki33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki34Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP38925.
PRIDEiP38925.
TopDownProteomicsiP38925.

PTM databases

iPTMnetiP38925.

Interactioni

Protein-protein interaction databases

BioGridi34279. 30 interactors.
IntActiP38925. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliP38925.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NRAMP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000006526.
HOGENOMiHOG000152204.
InParanoidiP38925.
KOiK12346.
OMAiMEIESHI.
OrthoDBiEOG092C1BTT.

Family and domain databases

HAMAPiMF_00221. NRAMP. 1 hit.
InterProiIPR001046. NRAMP_fam.
[Graphical view]
PANTHERiPTHR11706. PTHR11706. 3 hits.
PfamiPF01566. Nramp. 1 hit.
[Graphical view]
PRINTSiPR00447. NATRESASSCMP.
TIGRFAMsiTIGR01197. nramp. 1 hit.

Sequencei

Sequence statusi: Complete.

P38925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNVGPSHAA VAVDASEARK RNISEEVFEL RDKKDSTVVI EGEAPVRTFT
60 70 80 90 100
SSSSNHERED TYVSKRQVMR DIFAKYLKFI GPGLMVSVAY IDPGNYSTAV
110 120 130 140 150
DAGASNQFSL LCIILLSNFI AIFLQCLCIK LGSVTGLDLS RACREYLPRW
160 170 180 190 200
LNWTLYFFAE CAVIATDIAE VIGTAIALNI LIKVPLPAGV AITVVDVFLI
210 220 230 240 250
MFTYKPGASS IRFIRIFECF VAVLVVGVCI CFAIELAYIP KSTSVKQVFR
260 270 280 290 300
GFVPSAQMFD HNGIYTAISI LGATVMPHSL FLGSALVQPR LLDYDVKHGN
310 320 330 340 350
YTVSEEQDKV KKSKSTEEIM EEKYFNYRPT NAAIKYCMKY SMVELSITLF
360 370 380 390 400
TLALFVNCAI LVVAGSTLYN SPEADGADLF TIHELLSRNL APAAGTIFML
410 420 430 440 450
ALLLSGQSAG VVCTMSGQIV SEGHINWKLQ PWQRRLATRC ISIIPCLVIS
460 470 480 490 500
ICIGREALSK ALNASQVVLS IVLPFLVAPL IFFTCKKSIM KTEITVDHTE
510 520 530 540 550
EDSHNHQNNN DRSAGSVIEQ DGSSGMEIEN GKDVKIVYMA NNWIITVIAI
560 570
IVWLFLSLLN VYAIVQLGMS HGDIS
Length:575
Mass (Da):63,264
Last modified:November 1, 1997 - v2
Checksum:iAC0EEEF1BBF34ACB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti305E → D (PubMed:1447206).Curated1
Sequence conflicti416S → A (PubMed:1447206).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15929 Genomic DNA. Translation: AAB48984.1.
X95258 Genomic DNA. Translation: CAA64547.1.
Z74864 Genomic DNA. Translation: CAA99141.1.
BK006948 Genomic DNA. Translation: DAA10662.1.
PIRiS58647.
RefSeqiNP_014519.1. NM_001183376.1.

Genome annotation databases

EnsemblFungiiYOL122C; YOL122C; YOL122C.
GeneIDi854027.
KEGGisce:YOL122C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15929 Genomic DNA. Translation: AAB48984.1.
X95258 Genomic DNA. Translation: CAA64547.1.
Z74864 Genomic DNA. Translation: CAA99141.1.
BK006948 Genomic DNA. Translation: DAA10662.1.
PIRiS58647.
RefSeqiNP_014519.1. NM_001183376.1.

3D structure databases

ProteinModelPortaliP38925.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34279. 30 interactors.
IntActiP38925. 3 interactors.

Protein family/group databases

TCDBi2.A.55.1.1. the metal ion (mn(2+)-iron) transporter (nramp) family.

PTM databases

iPTMnetiP38925.

Proteomic databases

MaxQBiP38925.
PRIDEiP38925.
TopDownProteomicsiP38925.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL122C; YOL122C; YOL122C.
GeneIDi854027.
KEGGisce:YOL122C.

Organism-specific databases

EuPathDBiFungiDB:YOL122C.
SGDiS000005482. SMF1.

Phylogenomic databases

GeneTreeiENSGT00390000006526.
HOGENOMiHOG000152204.
InParanoidiP38925.
KOiK12346.
OMAiMEIESHI.
OrthoDBiEOG092C1BTT.

Enzyme and pathway databases

BioCyciYEAST:G3O-33518-MONOMER.
ReactomeiR-SCE-1222556. ROS, RNS production in response to bacteria.
R-SCE-425410. Metal ion SLC transporters.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-6803544. Ion influx/efflux at host-pathogen interface.
R-SCE-917937. Iron uptake and transport.

Miscellaneous databases

PROiP38925.

Family and domain databases

HAMAPiMF_00221. NRAMP. 1 hit.
InterProiIPR001046. NRAMP_fam.
[Graphical view]
PANTHERiPTHR11706. PTHR11706. 3 hits.
PfamiPF01566. Nramp. 1 hit.
[Graphical view]
PRINTSiPR00447. NATRESASSCMP.
TIGRFAMsiTIGR01197. nramp. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSMF1_YEAST
AccessioniPrimary (citable) accession number: P38925
Secondary accession number(s): D6W1U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.