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P38925

- SMF1_YEAST

UniProt

P38925 - SMF1_YEAST

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Protein

Manganese transporter SMF1

Gene

SMF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

High-affinity manganese transporter involved in manganese uptake from the extracellular environment. Contributes also to cellular accumulation of other divalent metal ions such as cadmium, cobalt, copper, iron and nickel.2 Publications

GO - Molecular functioni

  1. inorganic cation transmembrane transporter activity Source: SGD
  2. metal ion binding Source: UniProtKB-KW
  3. solute:proton symporter activity Source: SGD

GO - Biological processi

  1. cadmium ion transport Source: SGD
  2. cation transmembrane transport Source: GOC
  3. cellular cadmium ion homeostasis Source: SGD
  4. cellular copper ion homeostasis Source: SGD
  5. cellular manganese ion homeostasis Source: SGD
  6. copper ion transport Source: SGD
  7. iron ion transport Source: SGD
  8. manganese ion transport Source: SGD
  9. proton transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33518-MONOMER.

Protein family/group databases

TCDBi2.A.55.1.1. the metal ion (mn(2+)-iron) transporter (nramp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese transporter SMF1
Gene namesi
Name:SMF1
Synonyms:ESP1
Ordered Locus Names:YOL122C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL122c.
SGDiS000005482. SMF1.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Targeted to the vacuolar lumen in presence of excess manganese, where it is degraded.

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575Manganese transporter SMF1PRO_0000212605Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38925.
PaxDbiP38925.

Expressioni

Gene expression databases

GenevestigatoriP38925.

Interactioni

Protein-protein interaction databases

BioGridi34279. 31 interactions.
IntActiP38925. 3 interactions.
STRINGi4932.YOL122C.

Structurei

3D structure databases

ProteinModelPortaliP38925.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7070ExtracellularSequence AnalysisAdd
BLAST
Topological domaini92 – 10817CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini130 – 15627ExtracellularSequence AnalysisAdd
BLAST
Topological domaini178 – 1792CytoplasmicSequence Analysis
Topological domaini201 – 21818ExtracellularSequence AnalysisAdd
BLAST
Topological domaini240 – 26627CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini288 – 34457ExtracellularSequence AnalysisAdd
BLAST
Topological domaini366 – 39631CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini418 – 46346ExtracellularSequence AnalysisAdd
BLAST
Topological domaini485 – 54359CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini565 – 57511ExtracellularSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei71 – 9121HelicalSequence AnalysisAdd
BLAST
Transmembranei109 – 12921HelicalSequence AnalysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence AnalysisAdd
BLAST
Transmembranei180 – 20021HelicalSequence AnalysisAdd
BLAST
Transmembranei219 – 23921HelicalSequence AnalysisAdd
BLAST
Transmembranei267 – 28721HelicalSequence AnalysisAdd
BLAST
Transmembranei345 – 36521HelicalSequence AnalysisAdd
BLAST
Transmembranei397 – 41721HelicalSequence AnalysisAdd
BLAST
Transmembranei464 – 48421HelicalSequence AnalysisAdd
BLAST
Transmembranei544 – 56421HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the NRAMP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1914.
GeneTreeiENSGT00390000006526.
HOGENOMiHOG000152204.
InParanoidiP38925.
KOiK12346.
OMAiLMRGWRV.
OrthoDBiEOG7NSBBM.

Family and domain databases

HAMAPiMF_00221. NRAMP.
InterProiIPR001046. NRAMP_fam.
[Graphical view]
PANTHERiPTHR11706. PTHR11706. 1 hit.
PfamiPF01566. Nramp. 1 hit.
[Graphical view]
PRINTSiPR00447. NATRESASSCMP.
TIGRFAMsiTIGR01197. nramp. 1 hit.

Sequencei

Sequence statusi: Complete.

P38925-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNVGPSHAA VAVDASEARK RNISEEVFEL RDKKDSTVVI EGEAPVRTFT
60 70 80 90 100
SSSSNHERED TYVSKRQVMR DIFAKYLKFI GPGLMVSVAY IDPGNYSTAV
110 120 130 140 150
DAGASNQFSL LCIILLSNFI AIFLQCLCIK LGSVTGLDLS RACREYLPRW
160 170 180 190 200
LNWTLYFFAE CAVIATDIAE VIGTAIALNI LIKVPLPAGV AITVVDVFLI
210 220 230 240 250
MFTYKPGASS IRFIRIFECF VAVLVVGVCI CFAIELAYIP KSTSVKQVFR
260 270 280 290 300
GFVPSAQMFD HNGIYTAISI LGATVMPHSL FLGSALVQPR LLDYDVKHGN
310 320 330 340 350
YTVSEEQDKV KKSKSTEEIM EEKYFNYRPT NAAIKYCMKY SMVELSITLF
360 370 380 390 400
TLALFVNCAI LVVAGSTLYN SPEADGADLF TIHELLSRNL APAAGTIFML
410 420 430 440 450
ALLLSGQSAG VVCTMSGQIV SEGHINWKLQ PWQRRLATRC ISIIPCLVIS
460 470 480 490 500
ICIGREALSK ALNASQVVLS IVLPFLVAPL IFFTCKKSIM KTEITVDHTE
510 520 530 540 550
EDSHNHQNNN DRSAGSVIEQ DGSSGMEIEN GKDVKIVYMA NNWIITVIAI
560 570
IVWLFLSLLN VYAIVQLGMS HGDIS
Length:575
Mass (Da):63,264
Last modified:November 1, 1997 - v2
Checksum:iAC0EEEF1BBF34ACB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti305 – 3051E → D(PubMed:1447206)Curated
Sequence conflicti416 – 4161S → A(PubMed:1447206)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15929 Genomic DNA. Translation: AAB48984.1.
X95258 Genomic DNA. Translation: CAA64547.1.
Z74864 Genomic DNA. Translation: CAA99141.1.
BK006948 Genomic DNA. Translation: DAA10662.1.
PIRiS58647.
RefSeqiNP_014519.1. NM_001183376.1.

Genome annotation databases

EnsemblFungiiYOL122C; YOL122C; YOL122C.
GeneIDi854027.
KEGGisce:YOL122C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15929 Genomic DNA. Translation: AAB48984.1 .
X95258 Genomic DNA. Translation: CAA64547.1 .
Z74864 Genomic DNA. Translation: CAA99141.1 .
BK006948 Genomic DNA. Translation: DAA10662.1 .
PIRi S58647.
RefSeqi NP_014519.1. NM_001183376.1.

3D structure databases

ProteinModelPortali P38925.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34279. 31 interactions.
IntActi P38925. 3 interactions.
STRINGi 4932.YOL122C.

Protein family/group databases

TCDBi 2.A.55.1.1. the metal ion (mn(2+)-iron) transporter (nramp) family.

Proteomic databases

MaxQBi P38925.
PaxDbi P38925.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL122C ; YOL122C ; YOL122C .
GeneIDi 854027.
KEGGi sce:YOL122C.

Organism-specific databases

CYGDi YOL122c.
SGDi S000005482. SMF1.

Phylogenomic databases

eggNOGi COG1914.
GeneTreei ENSGT00390000006526.
HOGENOMi HOG000152204.
InParanoidi P38925.
KOi K12346.
OMAi LMRGWRV.
OrthoDBi EOG7NSBBM.

Enzyme and pathway databases

BioCyci YEAST:G3O-33518-MONOMER.

Miscellaneous databases

NextBioi 975571.

Gene expression databases

Genevestigatori P38925.

Family and domain databases

HAMAPi MF_00221. NRAMP.
InterProi IPR001046. NRAMP_fam.
[Graphical view ]
PANTHERi PTHR11706. PTHR11706. 1 hit.
Pfami PF01566. Nramp. 1 hit.
[Graphical view ]
PRINTSi PR00447. NATRESASSCMP.
TIGRFAMsi TIGR01197. nramp. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two related genes encoding extremely hydrophobic proteins suppress a lethal mutation in the yeast mitochondrial processing enhancing protein."
    West A.H., Clark D.J., Martin J., Neupert W., Hartl F.-U., Horwich A.L.
    J. Biol. Chem. 267:24625-24633(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A yeast manganese transporter related to the macrophage protein involved in conferring resistance to mycobacteria."
    Supek F., Supekova L., Nelson H., Nelson N.
    Proc. Natl. Acad. Sci. U.S.A. 93:5105-5110(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 200060 / W303.
  3. "DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames."
    Lafuente M.J., Gamo F.-J., Gancedo C.
    Yeast 12:1041-1045(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "The family of SMF metal ion transporters in yeast cells."
    Cohen A., Nelson H., Nelson N.
    J. Biol. Chem. 275:33388-33394(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Saccharomyces cerevisiae expresses three functionally distinct homologues of the nramp family of metal transporters."
    Portnoy M.E., Liu X.F., Culotta V.C.
    Mol. Cell. Biol. 20:7893-7902(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  9. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSMF1_YEAST
AccessioniPrimary (citable) accession number: P38925
Secondary accession number(s): D6W1U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3