P38922 (HRB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein HRB1

Alternative name(s):
Protein TOM34

Gene names

Name:	HRB1
Synonyms:	TOM34
Ordered Locus Names:	YNL004W
ORF Names:	N2009

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus Ref.8.
Miscellaneous	Present with 1990 molecules/cell in log phase SD medium.
Sequence similarities	Contains 3 RRM (RNA recognition motif) domains.
Sequence caution	The sequence AAA64803.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA54378.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA95863.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	poly(A)+ mRNA export from nucleus Inferred from physical interaction PubMed 14769921. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 9499403. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD nucleus Inferred from direct assay PubMed 9499403. Source: SGD
Molecular_function	RNA binding Inferred from direct assay PubMed 14676199. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Protein HRB1		PRO_0000081612
Regions
	Domain	161 – 237	77	RRM 1
	Domain	261 – 338	78	RRM 2
	Domain	376 – 453	78	RRM 3
	Compositional bias	13 – 127	115	Arg-rich
Amino acid modifications
	Modified residue	27	1	Phosphoserine Ref.11
	Modified residue	338	1	Phosphoserine Ref.12
	Modified residue	343	1	Phosphoserine Ref.10 Ref.12
	Modified residue	355	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P38922 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: C54D858C77076583	FASTA	454	52,142
	10 20 30 40 50 60 MSDQERGSEN NNRSRSRSRS PVRRRMSDDH GYERDNHLSR RSGNYNGRRK FADTYRGSRD 70 80 90 100 110 120 RGEYRGGRER SDYRERERFN NRDNPRSRDR YDDRRRGRDV TGRYGNRRDD YPRSFRSRHN 130 140 150 160 170 180 TRDDSRRGGF GSSGARGDYG PLLARELDST YEEKVNRNYS NSIFVGNLTY DSTPEDLTEF 190 200 210 220 230 240 FSQIGKVVRA DIITSRGHHR GMGTVEFTNS DDVDRAIRQY DGAFFMDRKI FVRQDNPPPS 250 260 270 280 290 300 NNIKERKALD RGELRHNRKT HEVIVKNLPA SVNWQALKDI FKECGNVAHA DVELDGDGVS 310 320 330 340 350 360 TGSGTVSFYD IKDLHRAIEK YNGYSIEGNV LDVKSKESVH NHSDGDDVDI PMDDSPVNEE 370 380 390 400 410 420 ARKFTENVVG GGERNRLIYC SNLPFSTAKS DLYDLFETIG KVNNAELRYD SKGAPTGIAV 430 440 450 VEYDNVDDAD VCIERLNNYN YGGCDLDISY AKRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two yeast chromosomes are related by a fossil duplication of their centromeric regions." Lalo D., Stettler S., Mariotte S., Slonimski P.P., Thuriaux P. C. R. Acad. Sci. III, Sci. Vie 316:367-373(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae." Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P. Yeast 10:523-533(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[3]	"Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence." Verhasselt P., Aert R., Voet M., Volckaert G. Yeast 10:945-951(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Genome-wide identification of spliced introns using a tiling microarray." Zhang Z., Hesselberth J.R., Fields S. Genome Res. 17:503-509(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-134. Strain: ATCC 201390 / BY4743.
[7]	"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing." Juneau K., Palm C., Miranda M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-119. Strain: ATCC 201390 / BY4743.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-343 AND SER-355, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338 AND SER-343, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U02536 Genomic DNA. Translation: AAA64803.1. Sequence problems. X77114 Genomic DNA. Translation: CAA54378.1. Sequence problems. Z71280 Genomic DNA. Translation: CAA95863.1. Sequence problems. DQ881449 mRNA. Translation: ABI95876.1. EF123128 mRNA. Translation: ABM97472.1. BK006947 Genomic DNA. Translation: DAA10539.1.
PIR	S45459.
RefSeq	NP_014394.2. NM_001182843.1.
3D structure databases
ProteinModelPortal	P38922.
SMR	P38922. Positions 162-345, 374-452.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4959N.
IntAct	P38922. 23 interactions.
MINT	MINT-475842.
Proteomic databases
PaxDb	P38922.
PeptideAtlas	P38922.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL004W; YNL004W; YNL004W.
GeneID	855728.
KEGG	sce:YNL004W.
Organism-specific databases
CYGD	YNL004w.
SGD	S000004949. HRB1.
Phylogenomic databases
eggNOG	COG0724.
GeneTree	ENSGT00410000025635.
HOGENOM	HOG000111155.
OMA	KTHEVIV.
OrthoDB	EOG4F21C2.
Gene expression databases
Genevestigator	P38922.
GermOnline	YNL004W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.70.330. 3 hits.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 3 hits. [Graphical view]
SMART	SM00360. RRM. 3 hits. [Graphical view]
PROSITE	PS50102. RRM. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	980105.

Entry information

Entry name

HRB1_YEAST

Accession

Primary (citable) accession number: P38922
Secondary accession number(s): A2TBM5, D6W1H3, Q06HN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program