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Protein

Protein HRB1

Gene

HRB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • RNA binding Source: SGD

GO - Biological processi

  • nuclear mRNA surveillance Source: SGD
  • poly(A)+ mRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33046-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein HRB1
Alternative name(s):
Protein TOM34
Gene namesi
Name:HRB1
Synonyms:TOM34
Ordered Locus Names:YNL004W
ORF Names:N2009
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL004W.
SGDiS000004949. HRB1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Protein HRB1PRO_0000081612Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei343 – 3431PhosphoserineCombined sources
Modified residuei355 – 3551PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38922.
PeptideAtlasiP38922.

PTM databases

iPTMnetiP38922.

Interactioni

Protein-protein interaction databases

BioGridi35821. 66 interactions.
DIPiDIP-4959N.
IntActiP38922. 23 interactions.
MINTiMINT-475842.

Structurei

Secondary structure

1
454
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi161 – 1688Combined sources
Helixi174 – 1818Combined sources
Helixi182 – 1843Combined sources
Beta strandi187 – 1915Combined sources
Beta strandi203 – 2086Combined sources
Helixi212 – 2176Combined sources
Turni218 – 2225Combined sources
Beta strandi232 – 2343Combined sources
Beta strandi263 – 2653Combined sources
Helixi274 – 2829Combined sources
Beta strandi289 – 2913Combined sources
Beta strandi304 – 3074Combined sources
Helixi311 – 32111Combined sources
Beta strandi325 – 3306Combined sources
Beta strandi333 – 3353Combined sources
Helixi359 – 3635Combined sources
Turni364 – 3674Combined sources
Beta strandi368 – 3714Combined sources
Beta strandi376 – 3827Combined sources
Helixi389 – 3968Combined sources
Turni397 – 3993Combined sources
Beta strandi402 – 41312Combined sources
Beta strandi415 – 42511Combined sources
Helixi426 – 43510Combined sources
Turni436 – 4383Combined sources
Beta strandi447 – 4537Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MZRNMR-A144-236[»]
2MZSNMR-A262-358[»]
2MZTNMR-A357-454[»]
ProteinModelPortaliP38922.
SMRiP38922. Positions 162-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini161 – 23777RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini261 – 33878RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini376 – 45378RRM 3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 127115Arg-richAdd
BLAST

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00410000025635.
HOGENOMiHOG000111155.
InParanoidiP38922.
OMAiMDIDDYS.
OrthoDBiEOG76TB3G.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38922-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQERGSEN NNRSRSRSRS PVRRRMSDDH GYERDNHLSR RSGNYNGRRK
60 70 80 90 100
FADTYRGSRD RGEYRGGRER SDYRERERFN NRDNPRSRDR YDDRRRGRDV
110 120 130 140 150
TGRYGNRRDD YPRSFRSRHN TRDDSRRGGF GSSGARGDYG PLLARELDST
160 170 180 190 200
YEEKVNRNYS NSIFVGNLTY DSTPEDLTEF FSQIGKVVRA DIITSRGHHR
210 220 230 240 250
GMGTVEFTNS DDVDRAIRQY DGAFFMDRKI FVRQDNPPPS NNIKERKALD
260 270 280 290 300
RGELRHNRKT HEVIVKNLPA SVNWQALKDI FKECGNVAHA DVELDGDGVS
310 320 330 340 350
TGSGTVSFYD IKDLHRAIEK YNGYSIEGNV LDVKSKESVH NHSDGDDVDI
360 370 380 390 400
PMDDSPVNEE ARKFTENVVG GGERNRLIYC SNLPFSTAKS DLYDLFETIG
410 420 430 440 450
KVNNAELRYD SKGAPTGIAV VEYDNVDDAD VCIERLNNYN YGGCDLDISY

AKRL
Length:454
Mass (Da):52,142
Last modified:July 10, 2007 - v2
Checksum:iC54D858C77076583
GO

Sequence cautioni

The sequence AAA64803.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA54378.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA95863.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02536 Genomic DNA. Translation: AAA64803.1. Sequence problems.
X77114 Genomic DNA. Translation: CAA54378.1. Sequence problems.
Z71280 Genomic DNA. Translation: CAA95863.1. Sequence problems.
DQ881449 mRNA. Translation: ABI95876.1.
EF123128 mRNA. Translation: ABM97472.1.
BK006947 Genomic DNA. Translation: DAA10539.1.
PIRiS45459.
RefSeqiNP_014394.2. NM_001182843.1.

Genome annotation databases

EnsemblFungiiYNL004W; YNL004W; YNL004W.
GeneIDi855728.
KEGGisce:YNL004W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02536 Genomic DNA. Translation: AAA64803.1. Sequence problems.
X77114 Genomic DNA. Translation: CAA54378.1. Sequence problems.
Z71280 Genomic DNA. Translation: CAA95863.1. Sequence problems.
DQ881449 mRNA. Translation: ABI95876.1.
EF123128 mRNA. Translation: ABM97472.1.
BK006947 Genomic DNA. Translation: DAA10539.1.
PIRiS45459.
RefSeqiNP_014394.2. NM_001182843.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MZRNMR-A144-236[»]
2MZSNMR-A262-358[»]
2MZTNMR-A357-454[»]
ProteinModelPortaliP38922.
SMRiP38922. Positions 162-345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35821. 66 interactions.
DIPiDIP-4959N.
IntActiP38922. 23 interactions.
MINTiMINT-475842.

PTM databases

iPTMnetiP38922.

Proteomic databases

MaxQBiP38922.
PeptideAtlasiP38922.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL004W; YNL004W; YNL004W.
GeneIDi855728.
KEGGisce:YNL004W.

Organism-specific databases

EuPathDBiFungiDB:YNL004W.
SGDiS000004949. HRB1.

Phylogenomic databases

GeneTreeiENSGT00410000025635.
HOGENOMiHOG000111155.
InParanoidiP38922.
OMAiMDIDDYS.
OrthoDBiEOG76TB3G.

Enzyme and pathway databases

BioCyciYEAST:G3O-33046-MONOMER.

Miscellaneous databases

NextBioi980105.
PROiP38922.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two yeast chromosomes are related by a fossil duplication of their centromeric regions."
    Lalo D., Stettler S., Mariotte S., Slonimski P.P., Thuriaux P.
    C. R. Acad. Sci. III, Sci. Vie 316:367-373(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. "Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae."
    Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P.
    Yeast 10:523-533(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  3. "Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence."
    Verhasselt P., Aert R., Voet M., Volckaert G.
    Yeast 10:945-951(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Genome-wide identification of spliced introns using a tiling microarray."
    Zhang Z., Hesselberth J.R., Fields S.
    Genome Res. 17:503-509(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-134.
    Strain: ATCC 201390 / BY4743.
  7. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-119.
    Strain: ATCC 201390 / BY4743.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-343 AND SER-355, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-343, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-343 AND SER-355, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHRB1_YEAST
AccessioniPrimary (citable) accession number: P38922
Secondary accession number(s): A2TBM5, D6W1H3, Q06HN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 10, 2007
Last modified: May 11, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1990 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.