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Protein

Aflatoxin B1 aldehyde reductase member 3

Gene

Akr7a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. Probably involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.1 Publication

Miscellaneous

With succinic semialdehyde or 4-nitrobenzaldehyde as substrate, it exhibits a substantially greater specific activity with NADPH than with NADH Conversely, it has a 3-fold higher activity towards 2-carboxybenzaldehyde with NADH than with NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40NADP1 Publication1
Active sitei45Proton donor1
Sitei73Lowers pKa of active site TyrBy similarity1
Binding sitei109Substrate1 Publication1
Binding sitei165NADP1 Publication1
Binding sitei218NADP1 Publication1
Binding sitei228Substrate1 Publication1
Binding sitei231Substrate1 Publication1
Binding sitei327NADP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi139 – 140NADP1 Publication2
Nucleotide bindingi194 – 204NADP1 PublicationAdd BLAST11
Nucleotide bindingi286 – 294NADP1 Publication9

GO - Molecular functioni

  • alditol:NADP+ 1-oxidoreductase activity Source: RGD
  • aldo-keto reductase (NADP) activity Source: RGD

GO - Biological processi

  • aflatoxin catabolic process Source: RGD
  • aflatoxin metabolic process Source: RGD

Keywordsi

Molecular functionOxidoreductase
Biological processDetoxification
LigandNAD, NADP

Enzyme and pathway databases

ReactomeiR-RNO-5423646 Aflatoxin activation and detoxification
SABIO-RKP38918

Names & Taxonomyi

Protein namesi
Recommended name:
Aflatoxin B1 aldehyde reductase member 3 (EC:1.-.-.-)
Short name:
AFB1-AR
Alternative name(s):
Aflatoxin B1 aldehyde reductase member 1
Short name:
rAFAR1
Gene namesi
Name:Akr7a3
Synonyms:Afar, Akr7a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi628635 Akr7a3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2697

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000703741 – 327Aflatoxin B1 aldehyde reductase member 3Add BLAST327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP38918
PRIDEiP38918

PTM databases

iPTMnetiP38918
PhosphoSitePlusiP38918

Expressioni

Inductioni

By the phenolic antioxidant ethoxyquin.

Gene expression databases

BgeeiENSRNOG00000017899
GenevisibleiP38918 RN

Interactioni

Subunit structurei

Homodimer. Heterodimer with AKR7A2.2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024160

Structurei

Secondary structure

1327
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Turni16 – 18Combined sources3
Helixi21 – 33Combined sources13
Beta strandi38 – 40Combined sources3
Helixi45 – 48Combined sources4
Helixi49 – 54Combined sources6
Beta strandi68 – 74Combined sources7
Helixi84 – 97Combined sources14
Beta strandi103 – 108Combined sources6
Helixi117 – 129Combined sources13
Beta strandi132 – 140Combined sources9
Helixi143 – 156Combined sources14
Beta strandi161 – 167Combined sources7
Helixi174 – 176Combined sources3
Helixi179 – 186Combined sources8
Beta strandi189 – 193Combined sources5
Helixi197 – 202Combined sources6
Helixi207 – 211Combined sources5
Beta strandi216 – 221Combined sources6
Helixi225 – 232Combined sources8
Helixi235 – 252Combined sources18
Helixi259 – 269Combined sources11
Helixi275 – 277Combined sources3
Beta strandi280 – 283Combined sources4
Helixi288 – 297Combined sources10
Helixi305 – 318Combined sources14
Helixi319 – 321Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GVEX-ray1.38A/B1-327[»]
ProteinModelPortaliP38918
SMRiP38918
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38918

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IDYD Eukaryota
COG0667 LUCA
GeneTreeiENSGT00550000074567
HOGENOMiHOG000250286
HOVERGENiHBG050576
InParanoidiP38918
KOiK15303
OMAiQKRGIVM
OrthoDBiEOG091G0BTK
PhylomeDBiP38918
TreeFamiTF329173

Family and domain databases

CDDicd06660 Aldo_ket_red, 1 hit
Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR020471 Aldo/keto_reductase
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 1 hit
PRINTSiPR00069 ALDKETRDTASE
SUPFAMiSSF51430 SSF51430, 1 hit

Sequencei

Sequence statusi: Complete.

P38918-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQARPATVL GAMEMGRRMD VTSSSASVRA FLQRGHTEID TAFVYANGQS
60 70 80 90 100
ETILGDLGLG LGRSGCKVKI ATKAAPMFGK TLKPADVRFQ LETSLKRLQC
110 120 130 140 150
PRVDLFYLHF PDHGTPIEET LQACHQLHQE GKFVELGLSN YVSWEVAEIC
160 170 180 190 200
TLCKKNGWIM PTVYQGMYNA ITRQVETELF PCLRHFGLRF YAFNPLAGGL
210 220 230 240 250
LTGRYKYQDK DGKNPESRFF GNPFSQLYMD RYWKEEHFNG IALVEKALKT
260 270 280 290 300
TYGPTAPSMI SAAVRWMYHH SQLKGTQGDA VILGMSSLEQ LEQNLALVEE
310 320
GPLEPAVVDA FDQAWNLVAH ECPNYFR
Length:327
Mass (Da):36,747
Last modified:December 15, 2003 - v2
Checksum:iF0C908C014DF672D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126 – 127QL → HV in CAA52740 (PubMed:8234296).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74673 mRNA Translation: CAA52740.1
AF045464 mRNA Translation: AAD02413.1
AY230497
, AY230491, AY230492, AY230493, AY230494, AY230495, AY230496 Genomic DNA Translation: AAO48423.1
BC078872 mRNA Translation: AAH78872.2
BC089811 mRNA Translation: AAH89811.1
PIRiA48804
RefSeqiNP_037347.1, NM_013215.1
UniGeneiRn.6043

Genome annotation databases

EnsembliENSRNOT00000024160; ENSRNOP00000024160; ENSRNOG00000017899
GeneIDi26760
KEGGirno:26760
UCSCiRGD:628635 rat

Similar proteinsi

Entry informationi

Entry nameiARK73_RAT
AccessioniPrimary (citable) accession number: P38918
Secondary accession number(s): Q5EBB7, Q68FZ3, Q9QX16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 15, 2003
Last modified: May 23, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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