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Protein

Transcription factor SPT8

Gene

SPT8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required, directly or indirectly, for TATA-binding protein function at particular promoters. May promote a functional interaction between SPT3 and TATA-binding protein. Functions as a component of the transcription regulatory histone acetylation (HAT) complex SAGA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs).1 Publication

Miscellaneous

Present with 8430 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • TBP-class protein binding Source: SGD
  • transcription cofactor activity Source: SGD

GO - Biological processi

  • chromatin organization Source: SGD
  • histone acetylation Source: SGD
  • negative regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32211-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SPT8
Gene namesi
Name:SPT8
Ordered Locus Names:YLR055C
ORF Names:L2144
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR055C
SGDiS000004045 SPT8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512261 – 602Transcription factor SPT8Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei85PhosphothreonineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei123PhosphoserineCombined sources1
Modified residuei131PhosphoserineCombined sources1
Modified residuei451PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38915
PaxDbiP38915
PRIDEiP38915

PTM databases

iPTMnetiP38915

Interactioni

Subunit structurei

Component of the 1.8 MDa SAGA complex, which consists of at least of TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • TBP-class protein binding Source: SGD

Protein-protein interaction databases

BioGridi31330, 408 interactors
DIPiDIP-5805N
IntActiP38915, 183 interactors
MINTiP38915
STRINGi4932.YLR055C

Structurei

3D structure databases

ProteinModelPortaliP38915
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati173 – 212WD 1Add BLAST40
Repeati305 – 346WD 2Add BLAST42
Repeati415 – 454WD 3Add BLAST40
Repeati506 – 544WD 4Add BLAST39
Repeati560 – 600WD 5Add BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 76Asp/Glu-rich (highly acidic)Add BLAST76

Sequence similaritiesi

Belongs to the WD repeat SPT8 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000173633
InParanoidiP38915
KOiK11360
OMAiRHDEGKR
OrthoDBiEOG092C2671

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
SMARTiView protein in SMART
SM00320 WD40, 4 hits
SUPFAMiSSF50978 SSF50978, 3 hits
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P38915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEVDDILIN NQVVDDEEDD EEMLSGLEND SKQDLEGNDD GGEDEEDDDD
60 70 80 90 100
DDEDDDDDED EREDDDEQED DDGEDDAARM DKTATPTNEH QHDEQKAAAA
110 120 130 140 150
GAGGAGDSGD AVTKIGSEDV KLSDVDGGVG SREASSSTHE ASANGEVYEY
160 170 180 190 200
YKHMLNAAQI ADSYNIYPTA AIPIQTHVNA LAVSRGLKYL FLGGSDGYIR
210 220 230 240 250
KYDLLNTLEG KLSLTILQKH SLAESIQNAG ILQSYWENEI PQKKSEMKLS
260 270 280 290 300
ANKTDYEPKV SPVHSLEVQS ECLFILSGLQ NGGITMQGVR YMEGSIAHYF
310 320 330 340 350
KGRNGHTQIV NILRLNGQED RFLSGSWDKR LLEWDLQTGD IVNEFKKSRS
360 370 380 390 400
ELSSLEMRPL YSSVDVSGNV NSGKENENAD DDMDSLFGDE DEDEKQDAGN
410 420 430 440 450
EPVETGDGSN GEENKEQISE ESLNIVYDES VFMTSGLNGS VHIWDRRMTQ
460 470 480 490 500
SPALSLERGA GVPPWCLSAC WGVDGDHVYA GRRNACVEQF DLKMPSKPIH
510 520 530 540 550
NLKLPSISGP VSCVKAMPNN KHLLCASRDN IRLYNVEIAV DASNSTTKSS
560 570 580 590 600
KVPFLIVPGH HGGIISNLYL DPTSRFIIST SGNRGWQGNS TDTTLIYDID

LE
Length:602
Mass (Da):66,190
Last modified:February 1, 1995 - v1
Checksum:iDDE88155E7638B46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94955 Genomic DNA Translation: AAA53585.1
X94607 Genomic DNA Translation: CAA64302.1
Z73227 Genomic DNA Translation: CAA97585.1
BK006945 Genomic DNA Translation: DAA09373.1
PIRiS47898
RefSeqiNP_013156.1, NM_001181942.1

Genome annotation databases

EnsemblFungiiYLR055C; YLR055C; YLR055C
GeneIDi850744
KEGGisce:YLR055C

Similar proteinsi

Entry informationi

Entry nameiSPT8_YEAST
AccessioniPrimary (citable) accession number: P38915
Secondary accession number(s): D6VY57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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