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Protein

Eukaryotic translation initiation factor 1A

Gene

TIF11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.

Miscellaneous

Present with 35100 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • double-stranded RNA binding Source: SGD
  • ribosomal small subunit binding Source: SGD
  • translation initiation factor activity Source: SGD
  • translation initiation factor binding Source: SGD

GO - Biological processi

  • formation of cytoplasmic translation initiation complex Source: SGD
  • formation of translation preinitiation complex Source: SGD

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-32935-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 1A
Short name:
eIF-1A
Alternative name(s):
Eukaryotic translation initiation factor 4C
Short name:
eIF-4C
Gene namesi
Name:TIF11
Ordered Locus Names:YMR260C
ORF Names:YM8156.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR260C.
SGDiS000004873. TIF11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic stress granule Source: SGD
  • eukaryotic 43S preinitiation complex Source: SGD
  • eukaryotic 48S preinitiation complex Source: SGD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451131 – 153Eukaryotic translation initiation factor 1AAdd BLAST153

Proteomic databases

MaxQBiP38912.
PRIDEiP38912.

PTM databases

iPTMnetiP38912.

Interactioni

GO - Molecular functioni

  • translation initiation factor binding Source: SGD

Protein-protein interaction databases

BioGridi35438. 200 interactors.
DIPiDIP-4401N.
IntActiP38912. 3 interactors.
MINTiMINT-562037.
STRINGi4932.YMR260C.

Structurei

Secondary structure

1153
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 43Combined sources11
Beta strandi46 – 51Combined sources6
Beta strandi56 – 61Combined sources6
Helixi63 – 65Combined sources3
Beta strandi76 – 81Combined sources6
Beta strandi86 – 94Combined sources9
Helixi98 – 106Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J80electron microscopy3.75i1-153[»]
3J81electron microscopy4.00i1-153[»]
3JAMelectron microscopy3.46i1-153[»]
3JAPelectron microscopy4.90i1-153[»]
3JAQelectron microscopy6.00i1-153[»]
3WBKX-ray3.30C27-153[»]
ProteinModelPortaliP38912.
SMRiP38912.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 96S1-likeAdd BLAST75

Sequence similaritiesi

Belongs to the eIF-1A family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000008256.
HOGENOMiHOG000223675.
InParanoidiP38912.
KOiK03236.
OMAiCTFEFGD.
OrthoDBiEOG092C5KXX.

Family and domain databases

HAMAPiMF_00216. aIF_1A. 1 hit.
InterProiView protein in InterPro
IPR012340. NA-bd_OB-fold.
IPR006196. RNA-binding_domain_S1_IF1.
IPR001253. TIF_eIF-1A.
IPR018104. TIF_eIF-1A_CS.
PANTHERiPTHR21668. PTHR21668. 1 hit.
PfamiView protein in Pfam
PF01176. eIF-1a. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005579. TIF_eIF-1A. 1 hit.
SMARTiView protein in SMART
SM00652. eIF1a. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00523. eIF-1A. 1 hit.
PROSITEiView protein in PROSITE
PS01262. IF1A. 1 hit.
PS50832. S1_IF1_TYPE. 1 hit.

Sequencei

Sequence statusi: Complete.

P38912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKNTKGGK KGRRGKNDSD GPKRELIYKE EGQEYAQITK MLGNGRVEAS
60 70 80 90 100
CFDGNKRMAH IRGKLRKKVW MGQGDIILVS LRDFQDDQCD VVHKYNLDEA
110 120 130 140 150
RTLKNQGELP ENAKINETDN FGFESDEDVN FEFGNADEDD EEGEDEELDI

DDI
Length:153
Mass (Da):17,435
Last modified:February 1, 1995 - v1
Checksum:iAEF000104BBA3041
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11585 Genomic DNA. Translation: AAA82039.1.
Z49260 Genomic DNA. Translation: CAA89243.1.
AY557964 Genomic DNA. Translation: AAS56290.1.
BK006946 Genomic DNA. Translation: DAA10160.1.
PIRiS47943.
RefSeqiNP_013987.1. NM_001182767.1.

Genome annotation databases

EnsemblFungiiYMR260C; YMR260C; YMR260C.
GeneIDi855302.
KEGGisce:YMR260C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIF1A_YEAST
AccessioniPrimary (citable) accession number: P38912
Secondary accession number(s): D6W086
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 5, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names