Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P38911

- FKBP3_YEAST

UniProt

P38911 - FKBP3_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

FK506-binding nuclear protein

Gene

FPR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. FK506- and rapamycin-binding protein. Specifically binds nuclear localization sequences. May be involved in the assembly or folding of ribosomal proteins.

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

Enzyme regulationi

Inhibited by both FK506 and rapamycin.

GO - Molecular functioni

  1. FK506 binding Source: InterPro
  2. macrolide binding Source: SGD
  3. peptidyl-prolyl cis-trans isomerase activity Source: SGD

GO - Biological processi

  1. histone peptidyl-prolyl isomerization Source: InterPro
  2. meiotic recombination checkpoint Source: SGD
  3. nucleosome assembly Source: SGD
  4. protein folding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Rotamase

Enzyme and pathway databases

BioCyciYEAST:YML074C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
FK506-binding nuclear protein (EC:5.2.1.8)
Alternative name(s):
FKBP-70
Nucleolar proline isomerase
Peptidyl-prolyl cis-trans isomerase
Short name:
PPIase
Proline rotamase
Gene namesi
Name:FPR3
Synonyms:NPI46
Ordered Locus Names:YML074C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML074c.
SGDiS000004539. FPR3.

Subcellular locationi

GO - Cellular componenti

  1. nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 411410FK506-binding nuclear proteinPRO_0000075313Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei80 – 801Phosphoserine3 Publications
Modified residuei81 – 811Phosphoserine3 Publications
Modified residuei89 – 891Phosphothreonine1 Publication
Modified residuei184 – 1841Phosphotyrosine; by CK21 Publication
Modified residuei186 – 1861Phosphoserine; by CK21 Publication

Post-translational modificationi

Phosphorylated at tyrosine and dephosphorylated by the phosphotyrosine-specific phosphoprotein phosphatase PTP1.4 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP38911.
PaxDbiP38911.
PeptideAtlasiP38911.

Expressioni

Gene expression databases

GenevestigatoriP38911.

Interactioni

Subunit structurei

Interacts with NOP53.1 Publication

Protein-protein interaction databases

BioGridi35067. 71 interactions.
DIPiDIP-6578N.
IntActiP38911. 59 interactions.
MINTiMINT-673400.
STRINGi4932.YML074C.

Structurei

3D structure databases

ProteinModelPortaliP38911.
SMRiP38911. Positions 266-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini324 – 41188PPIase FKBP-typePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi256 – 27116Nuclear localization signalSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi60 – 8728Asp/Glu-rich (highly acidic)Add
BLAST
Compositional biasi88 – 9912Lys-rich (highly basic)Add
BLAST
Compositional biasi101 – 11919Asp/Glu-rich (highly acidic)Add
BLAST
Compositional biasi173 – 24876Asp/Glu-rich (highly acidic)Add
BLAST
Compositional biasi250 – 29849Lys-rich (highly basic)Add
BLAST

Sequence similaritiesi

Contains 1 PPIase FKBP-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0545.
GeneTreeiENSGT00530000064286.
HOGENOMiHOG000216214.
InParanoidiP38911.
KOiK14826.
OMAiKGECIKG.
OrthoDBiEOG77WWQ3.

Family and domain databases

InterProiIPR026257. FK506_BP.
IPR023566. PPIase_FKBP.
IPR001179. PPIase_FKBP_dom.
[Graphical view]
PANTHERiPTHR10516. PTHR10516. 1 hit.
PfamiPF00254. FKBP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001473. FK506-bp_FPR3. 1 hit.
PROSITEiPS50059. FKBP_PPIASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38911-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDLLPLATY SLNVEPYTPV PAIDVTMPIT VRITMAALNP EAIDEENKPS
60 70 80 90 100
TLRIIKRNPD FEDDDFLGGD FDEDEIDEES SEEEEEEKTQ KKKKSKGKKA
110 120 130 140 150
ESESEDDEED DDEDDEFQES VLLTLSPEAQ YQQSLDLTIT PEEEVQFIVT
160 170 180 190 200
GSYAISLSGN YVKHPFDTPM GVEGEDEDED ADIYDSEDYD LTPDEDEIIG
210 220 230 240 250
DDMDDLDDEE EEEVRIEEVQ EEDEEDNDGE EEQEEEEEEE QKEEVKPEPK
260 270 280 290 300
KSKKEKKRKH EEKEEEKKAK KVKKVEFKKD LEEGPTKPKS KKEQDKHKPK
310 320 330 340 350
SKVLEGGIVI EDRTIGDGPQ AKRGARVGMR YIGKLKNGKV FDKNTSGKPF
360 370 380 390 400
AFKLGRGEVI KGWDIGVAGM SVGGERRIII PAPYAYGKQA LPGIPANSEL
410
TFDVKLVSMK N
Length:411
Mass (Da):46,553
Last modified:November 1, 1995 - v2
Checksum:iA01D24DE0078FE11
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1221L → P in AAB04165. (PubMed:7925954)Curated
Sequence conflicti240 – 2401E → EEE(PubMed:7925954)Curated
Sequence conflicti335 – 3351L → F in AAB04165. (PubMed:7925954)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34569 Genomic DNA. Translation: AAB04165.1.
X79379 Genomic DNA. Translation: CAA55924.1.
S73876 Genomic DNA. Translation: AAB31995.1.
Z46373 Genomic DNA. Translation: CAA86504.1.
AY693136 Genomic DNA. Translation: AAT93155.1.
BK006946 Genomic DNA. Translation: DAA09823.1.
PIRiS48647.
RefSeqiNP_013637.1. NM_001182433.1.

Genome annotation databases

EnsemblFungiiYML074C; YML074C; YML074C.
GeneIDi854901.
KEGGisce:YML074C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34569 Genomic DNA. Translation: AAB04165.1 .
X79379 Genomic DNA. Translation: CAA55924.1 .
S73876 Genomic DNA. Translation: AAB31995.1 .
Z46373 Genomic DNA. Translation: CAA86504.1 .
AY693136 Genomic DNA. Translation: AAT93155.1 .
BK006946 Genomic DNA. Translation: DAA09823.1 .
PIRi S48647.
RefSeqi NP_013637.1. NM_001182433.1.

3D structure databases

ProteinModelPortali P38911.
SMRi P38911. Positions 266-410.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35067. 71 interactions.
DIPi DIP-6578N.
IntActi P38911. 59 interactions.
MINTi MINT-673400.
STRINGi 4932.YML074C.

Proteomic databases

MaxQBi P38911.
PaxDbi P38911.
PeptideAtlasi P38911.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML074C ; YML074C ; YML074C .
GeneIDi 854901.
KEGGi sce:YML074C.

Organism-specific databases

CYGDi YML074c.
SGDi S000004539. FPR3.

Phylogenomic databases

eggNOGi COG0545.
GeneTreei ENSGT00530000064286.
HOGENOMi HOG000216214.
InParanoidi P38911.
KOi K14826.
OMAi KGECIKG.
OrthoDBi EOG77WWQ3.

Enzyme and pathway databases

BioCyci YEAST:YML074C-MONOMER.

Miscellaneous databases

NextBioi 977879.

Gene expression databases

Genevestigatori P38911.

Family and domain databases

InterProi IPR026257. FK506_BP.
IPR023566. PPIase_FKBP.
IPR001179. PPIase_FKBP_dom.
[Graphical view ]
PANTHERi PTHR10516. PTHR10516. 1 hit.
Pfami PF00254. FKBP_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF001473. FK506-bp_FPR3. 1 hit.
PROSITEi PS50059. FKBP_PPIASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification of FKBP-70, a novel immunophilin from Saccharomyces cerevisiae, and cloning of its structural gene, FPR3."
    Manning-Krieg U.C., Henriquez R., Cammas F., Graff P., Gaveriaux S., Movva N.R.
    FEBS Lett. 352:98-103(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Yeast NPI46 encodes a novel prolyl cis-trans isomerase that is located in the nucleolus."
    Shan X., Xue Z., Melese T.
    J. Cell Biol. 126:853-862(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "A novel FK506- and rapamycin-binding protein (FPR3 gene product) in the yeast Saccharomyces cerevisiae is a proline rotamase localized to the nucleolus."
    Benton B.M., Zang J.-H., Thorner J.
    J. Cell Biol. 127:623-639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YNN 214.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Casein kinase II catalyzes tyrosine phosphorylation of the yeast nucleolar immunophilin Fpr3."
    Wilson L.K., Dhillon N., Thorner J., Martin G.S.
    J. Biol. Chem. 272:12961-12967(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-184 AND SER-186.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Nop53p is a novel nucleolar 60S ribosomal subunit biogenesis protein."
    Sydorskyy Y., Dilworth D.J., Halloran B., Yi E.C., Makhnevych T., Wozniak R.W., Aitchison J.D.
    Biochem. J. 388:819-826(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOP53, IDENTIFICATION BY MASS SPECTROMETRY.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-81, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-81, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-81 AND THR-89, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFKBP3_YEAST
AccessioniPrimary (citable) accession number: P38911
Secondary accession number(s): D6W0K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9490 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3