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Protein

10 kDa heat shock protein, mitochondrial

Gene

HSP10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.

GO - Molecular functioni

  • ATP binding Source: InterPro
  • chaperone binding Source: SGD
  • metal ion binding Source: GO_Central
  • unfolded protein binding Source: SGD

GO - Biological processi

  • chaperone-mediated protein complex assembly Source: SGD
  • chaperone mediated protein folding requiring cofactor Source: GO_Central
  • protein folding Source: SGD
  • protein import into mitochondrial intermembrane space Source: SGD
  • protein refolding Source: SGD
  • response to unfolded protein Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-33568-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
10 kDa heat shock protein, mitochondrial
Short name:
HSP10
Alternative name(s):
10 kDa chaperonin
Gene namesi
Name:HSP10
Synonyms:CPN10
Ordered Locus Names:YOR020C
ORF Names:OR26.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR020C.
SGDiS000005546. HSP10.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 10610510 kDa heat shock protein, mitochondrialPRO_0000174922Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei31 – 311PhosphoserineCombined sources

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP38910.
TopDownProteomicsiP38910.

PTM databases

iPTMnetiP38910.

Interactioni

Subunit structurei

Homohexamer.By similarity

GO - Molecular functioni

  • chaperone binding Source: SGD
  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi34424. 22 interactions.
DIPiDIP-1483N.
IntActiP38910. 9 interactions.
MINTiMINT-395559.

Structurei

3D structure databases

ProteinModelPortaliP38910.
SMRiP38910. Positions 6-102.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GroES chaperonin family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006350.
HOGENOMiHOG000133897.
InParanoidiP38910.
KOiK04078.
OMAiFGGTKVQ.
OrthoDBiEOG71CG07.

Family and domain databases

Gene3Di2.30.33.40. 1 hit.
HAMAPiMF_00580. CH10.
InterProiIPR020818. Chaperonin_GroES.
IPR018369. Chaprnonin_Cpn10_CS.
IPR011032. GroES-like.
[Graphical view]
PANTHERiPTHR10772. PTHR10772. 1 hit.
PfamiPF00166. Cpn10. 1 hit.
[Graphical view]
PRINTSiPR00297. CHAPERONIN10.
SMARTiSM00883. Cpn10. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
PROSITEiPS00681. CHAPERONINS_CPN10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38910-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLLKSAKS IVPLMDRVLV QRIKAQAKTA SGLYLPEKNV EKLNQAEVVA
60 70 80 90 100
VGPGFTDANG NKVVPQVKVG DQVLIPQFGG STIKLGNDDE VILFRDAEIL

AKIAKD
Length:106
Mass (Da):11,372
Last modified:February 1, 1995 - v1
Checksum:i0453269D502C05E8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821T → S in AAT93241 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76853 Genomic DNA. Translation: CAA54185.1.
X75754 Genomic DNA. Translation: CAA53382.1.
X87331 Genomic DNA. Translation: CAA60769.1.
Z74928 Genomic DNA. Translation: CAA99210.1.
AY693222 Genomic DNA. Translation: AAT93241.1.
BK006948 Genomic DNA. Translation: DAA10802.1.
PIRiS39463.
RefSeqiNP_014663.1. NM_001183439.1.

Genome annotation databases

EnsemblFungiiYOR020C; YOR020C; YOR020C.
GeneIDi854185.
KEGGisce:YOR020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76853 Genomic DNA. Translation: CAA54185.1.
X75754 Genomic DNA. Translation: CAA53382.1.
X87331 Genomic DNA. Translation: CAA60769.1.
Z74928 Genomic DNA. Translation: CAA99210.1.
AY693222 Genomic DNA. Translation: AAT93241.1.
BK006948 Genomic DNA. Translation: DAA10802.1.
PIRiS39463.
RefSeqiNP_014663.1. NM_001183439.1.

3D structure databases

ProteinModelPortaliP38910.
SMRiP38910. Positions 6-102.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34424. 22 interactions.
DIPiDIP-1483N.
IntActiP38910. 9 interactions.
MINTiMINT-395559.

PTM databases

iPTMnetiP38910.

Proteomic databases

MaxQBiP38910.
TopDownProteomicsiP38910.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR020C; YOR020C; YOR020C.
GeneIDi854185.
KEGGisce:YOR020C.

Organism-specific databases

EuPathDBiFungiDB:YOR020C.
SGDiS000005546. HSP10.

Phylogenomic databases

GeneTreeiENSGT00390000006350.
HOGENOMiHOG000133897.
InParanoidiP38910.
KOiK04078.
OMAiFGGTKVQ.
OrthoDBiEOG71CG07.

Enzyme and pathway databases

BioCyciYEAST:G3O-33568-MONOMER.

Miscellaneous databases

PROiP38910.

Family and domain databases

Gene3Di2.30.33.40. 1 hit.
HAMAPiMF_00580. CH10.
InterProiIPR020818. Chaperonin_GroES.
IPR018369. Chaprnonin_Cpn10_CS.
IPR011032. GroES-like.
[Graphical view]
PANTHERiPTHR10772. PTHR10772. 1 hit.
PfamiPF00166. Cpn10. 1 hit.
[Graphical view]
PRINTSiPR00297. CHAPERONIN10.
SMARTiSM00883. Cpn10. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
PROSITEiPS00681. CHAPERONINS_CPN10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and disruption of the gene encoding yeast mitochondrial chaperonin 10, the homolog of E. coli groES."
    Rospert S., Junne T., Glick B.S., Schatz G.
    FEBS Lett. 335:358-360(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Role of the chaperonin cofactor Hsp10 in protein folding and sorting in yeast mitochondria."
    Hoehfeld J., Hartl F.U.
    J. Cell Biol. 126:305-315(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Identification and functional analysis of chaperonin 10, the groES homolog from yeast mitochondria."
    Rospert S., Glick B.S., Jenoe P., Schatz G., Todd M.J., Lorimer G.H., Viitanen P.V.
    Proc. Natl. Acad. Sci. U.S.A. 90:10967-10971(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 10-24; 29-35 AND 63-78.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCH10_YEAST
AccessioniPrimary (citable) accession number: P38910
Secondary accession number(s): D6W286, E9P935
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 149 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.