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Protein

Inner kinetochore subunit CHL4

Gene

CHL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.1 Publication

Miscellaneous

Present with 606 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • structural molecule activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • establishment of meiotic sister chromatid cohesion Source: SGD
  • establishment of mitotic sister chromatid cohesion Source: SGD
  • kinetochore assembly Source: SGD
  • maintenance of meiotic sister chromatid cohesion Source: SGD
  • mitotic spindle assembly checkpoint Source: SGD
  • protein localization to chromosome, centromeric region Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29826-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Inner kinetochore subunit CHL4Curated
Alternative name(s):
CENP-N homolog1 Publication
Chromosome loss protein 4
Chromosome transmission fidelity protein 17
Constitutive centromere-associated network protein CHL4Curated
Minichromosome maintenance protein 17
Gene namesi
Name:CHL4
Synonyms:CTF17, MCM17
Ordered Locus Names:YDR254W
ORF Names:YD9320A.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR254W.
SGDiS000002662. CHL4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000896461 – 458Inner kinetochore subunit CHL4Add BLAST458

Proteomic databases

MaxQBiP38907.
PaxDbiP38907.
PRIDEiP38907.

Interactioni

Subunit structurei

Forms a heterodimer with IML3 (PubMed:12589047, PubMed:24075991). CHL4-IML3 is part of a larger constitutive centromere-associated network (CCAN) (also known as central kinetochore CTF19 complex in yeast), which is composed of at least AME1, CHL4, CNN1, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1, NKP2, OKP1 and WIP1 (PubMed:12408861, PubMed:22561346). Interacts with CTF3 and CTF19 (PubMed:12589047).4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32304. 192 interactors.
DIPiDIP-5659N.
IntActiP38907. 16 interactors.
MINTiP38907.
STRINGi4932.YDR254W.

Structurei

Secondary structure

1458
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi383 – 393Combined sources11
Beta strandi396 – 408Combined sources13
Helixi409 – 418Combined sources10
Helixi424 – 426Combined sources3
Turni429 – 437Combined sources9
Beta strandi440 – 443Combined sources4
Beta strandi446 – 449Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JE3X-ray2.28B361-458[»]
ProteinModelPortaliP38907.
SMRiP38907.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CENP-N/CHL4 family.Curated

Phylogenomic databases

HOGENOMiHOG000111630.
InParanoidiP38907.
KOiK11551.
OMAiDWRHGIT.
OrthoDBiEOG092C39J1.

Family and domain databases

InterProiView protein in InterPro
IPR007902. Chl4/mis15/CENP-N.
PANTHERiPTHR12309:SF12. PTHR12309:SF12. 1 hit.
PfamiView protein in Pfam
PF05238. CENP-N. 1 hit.

Sequencei

Sequence statusi: Complete.

P38907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNELRLEDN YVPTSDTLVV FKQLMKLPVT VLYDLTLSWF AKFGGSFDGD
60 70 80 90 100
IYLLTETLDL LIEKGVRRNV IVNRILYVYW PDGLNVFQLA EIDCHLMISK
110 120 130 140 150
PEKFKWLPSK ALRGDGKPYV VKLQPAKFIE NLQTDLAKIY HCHVYMFKHP
160 170 180 190 200
SLPVLITRIQ LFDSNNLFLS TPNIGSINKE SLYNKLDKFQ GKPLISRRPY
210 220 230 240 250
YVAFPLNSPI IFHSVDKDIY ARLVLQSISR TISERETIIF KPVQKIPVKS
260 270 280 290 300
IHNIMTLLGP SRFAESMGPW ECYASANFER SPLHDYKKHQ GLTGKKVMVR
310 320 330 340 350
EFDDSFLNDD ENFYGKEEPE IRRLRLEKNM IKFKGSANGV MDQKYNDLKE
360 370 380 390 400
FNEHVHNIRN GKKNEDSGEP VYISRYSSLV PIEKVGFTLK NEINSRIITI
410 420 430 440 450
KLKFNGNDIF GGLHELCDKN LINIDKVPGW LAGENGSFSG TIMNGDFQRE

QVAKGGLL
Length:458
Mass (Da):52,672
Last modified:February 1, 1995 - v1
Checksum:i7C8A62C1313DCD60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63181 Genomic DNA. Translation: AAB27309.1.
M92290 Unassigned DNA. Translation: AAA16452.1.
Z70202 Genomic DNA. Translation: CAA94093.1.
Z68329 Genomic DNA. Translation: CAA92711.1.
BK006938 Genomic DNA. Translation: DAA12094.1.
PIRiS52594.
RefSeqiNP_010540.3. NM_001180562.3.

Genome annotation databases

EnsemblFungiiYDR254W; YDR254W; YDR254W.
GeneIDi851841.
KEGGisce:YDR254W.

Similar proteinsi

Entry informationi

Entry nameiCENPN_YEAST
AccessioniPrimary (citable) accession number: P38907
Secondary accession number(s): D6VSN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome