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Protein

Central kinetochore subunit CHL4

Gene

CHL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.1 Publication

GO - Molecular functioni

  • structural molecule activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • establishment of meiotic sister chromatid cohesion Source: SGD
  • establishment of mitotic sister chromatid cohesion Source: SGD
  • kinetochore assembly Source: SGD
  • maintenance of meiotic sister chromatid cohesion Source: SGD
  • mitotic spindle assembly checkpoint Source: SGD
  • protein localization to chromosome, centromeric region Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29826-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Central kinetochore subunit CHL4
Alternative name(s):
Chromosome loss protein 4
Chromosome transmission fidelity protein 17
Minichromosome maintenance protein 17
Gene namesi
Name:CHL4
Synonyms:CTF17, MCM17
Ordered Locus Names:YDR254W
ORF Names:YD9320A.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR254W.
SGDiS000002662. CHL4.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome outer kinetochore Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000896461 – 458Central kinetochore subunit CHL4Add BLAST458

Proteomic databases

MaxQBiP38907.
PRIDEiP38907.

Interactioni

Subunit structurei

Component of the central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1. Interacts with CTF3, CTF19 and IML3.1 Publication

Protein-protein interaction databases

BioGridi32304. 140 interactors.
DIPiDIP-5659N.
IntActiP38907. 7 interactors.
MINTiMINT-547226.

Structurei

Secondary structure

1458
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi383 – 393Combined sources11
Beta strandi396 – 408Combined sources13
Helixi409 – 418Combined sources10
Helixi424 – 426Combined sources3
Turni429 – 437Combined sources9
Beta strandi440 – 443Combined sources4
Beta strandi446 – 449Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JE3X-ray2.28B361-458[»]
ProteinModelPortaliP38907.
SMRiP38907.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000111630.
InParanoidiP38907.
KOiK11551.
OMAiDCQLIVD.
OrthoDBiEOG092C39J1.

Family and domain databases

InterProiIPR007902. Chl4/mis15/CENP-N.
[Graphical view]
PfamiPF05238. CENP-N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNELRLEDN YVPTSDTLVV FKQLMKLPVT VLYDLTLSWF AKFGGSFDGD
60 70 80 90 100
IYLLTETLDL LIEKGVRRNV IVNRILYVYW PDGLNVFQLA EIDCHLMISK
110 120 130 140 150
PEKFKWLPSK ALRGDGKPYV VKLQPAKFIE NLQTDLAKIY HCHVYMFKHP
160 170 180 190 200
SLPVLITRIQ LFDSNNLFLS TPNIGSINKE SLYNKLDKFQ GKPLISRRPY
210 220 230 240 250
YVAFPLNSPI IFHSVDKDIY ARLVLQSISR TISERETIIF KPVQKIPVKS
260 270 280 290 300
IHNIMTLLGP SRFAESMGPW ECYASANFER SPLHDYKKHQ GLTGKKVMVR
310 320 330 340 350
EFDDSFLNDD ENFYGKEEPE IRRLRLEKNM IKFKGSANGV MDQKYNDLKE
360 370 380 390 400
FNEHVHNIRN GKKNEDSGEP VYISRYSSLV PIEKVGFTLK NEINSRIITI
410 420 430 440 450
KLKFNGNDIF GGLHELCDKN LINIDKVPGW LAGENGSFSG TIMNGDFQRE

QVAKGGLL
Length:458
Mass (Da):52,672
Last modified:February 1, 1995 - v1
Checksum:i7C8A62C1313DCD60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63181 Genomic DNA. Translation: AAB27309.1.
M92290 Unassigned DNA. Translation: AAA16452.1.
Z70202 Genomic DNA. Translation: CAA94093.1.
Z68329 Genomic DNA. Translation: CAA92711.1.
BK006938 Genomic DNA. Translation: DAA12094.1.
PIRiS52594.
RefSeqiNP_010540.3. NM_001180562.3.

Genome annotation databases

EnsemblFungiiYDR254W; YDR254W; YDR254W.
GeneIDi851841.
KEGGisce:YDR254W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63181 Genomic DNA. Translation: AAB27309.1.
M92290 Unassigned DNA. Translation: AAA16452.1.
Z70202 Genomic DNA. Translation: CAA94093.1.
Z68329 Genomic DNA. Translation: CAA92711.1.
BK006938 Genomic DNA. Translation: DAA12094.1.
PIRiS52594.
RefSeqiNP_010540.3. NM_001180562.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JE3X-ray2.28B361-458[»]
ProteinModelPortaliP38907.
SMRiP38907.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32304. 140 interactors.
DIPiDIP-5659N.
IntActiP38907. 7 interactors.
MINTiMINT-547226.

Proteomic databases

MaxQBiP38907.
PRIDEiP38907.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR254W; YDR254W; YDR254W.
GeneIDi851841.
KEGGisce:YDR254W.

Organism-specific databases

EuPathDBiFungiDB:YDR254W.
SGDiS000002662. CHL4.

Phylogenomic databases

HOGENOMiHOG000111630.
InParanoidiP38907.
KOiK11551.
OMAiDCQLIVD.
OrthoDBiEOG092C39J1.

Enzyme and pathway databases

BioCyciYEAST:G3O-29826-MONOMER.

Miscellaneous databases

PROiP38907.

Family and domain databases

InterProiIPR007902. Chl4/mis15/CENP-N.
[Graphical view]
PfamiPF05238. CENP-N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHL4_YEAST
AccessioniPrimary (citable) accession number: P38907
Secondary accession number(s): D6VSN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 606 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.