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P38902 (RPB11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase II subunit RPB11

Short name=RNA polymerase II subunit B11
Alternative name(s):
B13.6
DNA-directed RNA polymerase II 13.6 kDa polypeptide
Gene names
Name:RPB11
Ordered Locus Names:YOL005C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length120 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft. Seems to be involved transcript termination. Ref.6

Subunit structure

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.

Subcellular location

Nucleus.

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RPB3P163704EBI-15806,EBI-15773

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 120120DNA-directed RNA polymerase II subunit RPB11
PRO_0000149315

Experimental info

Mutagenesis1081E → G or V: Transcript termination readthrough. Ref.6
Mutagenesis1081E → K: Transcript termination readthrough. Lethal. Ref.6
Mutagenesis1111L → P: Transcript termination readthrough. Ref.6
Mutagenesis1141L → P: Transcript termination readthrough. Ref.6

Secondary structure

...................... 120
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38902 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: A98D109C5FF8E356

FASTA12013,616
        10         20         30         40         50         60 
MNAPDRFELF LLGEGESKLK IDPDTKAPNA VVITFEKEDH TLGNLIRAEL LNDRKVLFAA 

        70         80         90        100        110        120 
YKVEHPFFAR FKLRIQTTEG YDPKDALKNA CNSIINKLGA LKTNFETEWN LQTLAADDAF 

« Hide

References

« Hide 'large scale' references
[1]"Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III."
Woychik N.A., McKune K., Lane W.S., Young R.A.
Gene Expr. 3:77-82(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-37; 75-84; 98-102 AND 103-116.
Strain: X2180-2.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"cis- and trans-Acting determinants of transcription termination by yeast RNA polymerase II."
Steinmetz E.J., Ng S.B., Cloute J.P., Brow D.A.
Mol. Cell. Biol. 26:2688-2696(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLU-108; LEU-111 AND LEU-114.
[7]"RNA polymerase II/TFIIF structure and conserved organization of the initiation complex."
Chung W.H., Craighead J.L., Chang W.H., Ezeokonkwo C., Bareket-Samish A., Kornberg R.D., Asturias F.J.
Mol. Cell 12:1003-1013(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ELECTRON MICROSCOPY OF THE RNA POL II/TFIIF COMPLEX.
[8]"Structural basis of transcription: RNA polymerase II at 2.8 A resolution."
Cramer P., Bushnell D.A., Kornberg R.D.
Science 292:1863-1876(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
[9]"Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution."
Gnatt A.L., Cramer P., Fu J., Bushnell D.A., Kornberg R.D.
Science 292:1876-1882(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
[10]"Structural basis of transcription: alpha-amanitin-RNA polymerase II cocrystal at 2.8 A resolution."
Bushnell D.A., Cramer P., Kornberg R.D.
Proc. Natl. Acad. Sci. U.S.A. 99:1218-1222(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE RNA POL II CORE COMPLEX IN COMPLEX WITH ALPHA-AMANITIN.
[11]"Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavage."
Kettenberger H., Armache K.J., Cramer P.
Cell 114:347-357(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX IN COMPLEX WITH DST1.
[12]"Architecture of initiation-competent 12-subunit RNA polymerase II."
Armache K.J., Kettenberger H., Cramer P.
Proc. Natl. Acad. Sci. U.S.A. 100:6964-6968(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.2 ANGSTROMS) OF THE RNA POL II COMPLEX.
[13]"Complete, 12-subunit RNA polymerase II at 4.1-A resolution: implications for the initiation of transcription."
Bushnell D.A., Kornberg R.D.
Proc. Natl. Acad. Sci. U.S.A. 100:6969-6973(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
[14]"Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center."
Westover K.D., Bushnell D.A., Kornberg R.D.
Cell 119:481-489(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
[15]"Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS."
Kettenberger H., Armache K.J., Cramer P.
Mol. Cell 16:955-965(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.5 ANGSTROMS).
[16]"Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms."
Bushnell D.A., Westover K.D., Davis R.E., Kornberg R.D.
Science 303:983-988(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.5 ANGSTROMS) OF THE RNA POL II CORE COMPLEX.
[17]"Structures of complete RNA polymerase II and its subcomplex, Rpb4/7."
Armache K.J., Mitterweger S., Meinhart A., Cramer P.
J. Biol. Chem. 280:7131-7134(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX.
[18]"Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs."
Kettenberger H., Eisenfuhr A., Brueckner F., Theis M., Famulok M., Cramer P.
Nat. Struct. Mol. Biol. 13:44-48(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF THE RNA POL II COMPLEX IN COMPLEX WITH INHIBITING NON-CODING RNA.
[19]"Phasing RNA polymerase II using intrinsically bound Zn atoms: an updated structural model."
Meyer P.A., Ye P., Zhang M., Suh M.H., Fu J.
Structure 14:973-982(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.15 ANGSTROMS) OF THE RNA POL II COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S62624 Genomic DNA. Translation: AAB27135.1.
Z74747 Genomic DNA. Translation: CAA99004.1.
AY557998 Genomic DNA. Translation: AAS56324.1.
BK006948 Genomic DNA. Translation: DAA10778.1.
PIRS58933.
RefSeqNP_014638.1. NM_001183259.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1I3QX-ray3.10K1-120[»]
1I50X-ray2.80K1-120[»]
1I6HX-ray3.30K1-120[»]
1K83X-ray2.80K1-120[»]
1NIKX-ray4.10K1-120[»]
1NT9X-ray4.20K1-120[»]
1PQVX-ray3.80K1-120[»]
1R5UX-ray4.50K1-120[»]
1R9SX-ray4.25K1-120[»]
1R9TX-ray3.50K1-120[»]
1SFOX-ray3.61K1-120[»]
1TWAX-ray3.20K1-120[»]
1TWCX-ray3.00K1-120[»]
1TWFX-ray2.30K1-120[»]
1TWGX-ray3.30K1-120[»]
1TWHX-ray3.40K1-120[»]
1WCMX-ray3.80K1-120[»]
1Y1VX-ray3.80K1-120[»]
1Y1WX-ray4.00K1-120[»]
1Y1YX-ray4.00K1-120[»]
1Y77X-ray4.50K1-120[»]
2B63X-ray3.80K1-120[»]
2B8KX-ray4.15K1-120[»]
2E2HX-ray3.95K1-120[»]
2E2IX-ray3.41K1-120[»]
2E2JX-ray3.50K1-120[»]
2JA5X-ray3.80K1-120[»]
2JA6X-ray4.00K1-120[»]
2JA7X-ray3.80K/W1-120[»]
2JA8X-ray3.80K1-120[»]
2NVQX-ray2.90K1-120[»]
2NVTX-ray3.36K1-120[»]
2NVXX-ray3.60K1-120[»]
2NVYX-ray3.40K1-120[»]
2NVZX-ray4.30K1-120[»]
2R7ZX-ray3.80K1-120[»]
2R92X-ray3.80K1-120[»]
2R93X-ray4.00K1-120[»]
2VUMX-ray3.40K1-120[»]
2YU9X-ray3.40K1-120[»]
3CQZX-ray2.80K1-120[»]
3FKIX-ray3.88K1-120[»]
3GTGX-ray3.78K1-120[»]
3GTJX-ray3.42K1-120[»]
3GTKX-ray3.80K1-120[»]
3GTLX-ray3.38K1-120[»]
3GTMX-ray3.80K1-120[»]
3GTOX-ray4.00K1-120[»]
3GTPX-ray3.90K1-120[»]
3GTQX-ray3.80K1-120[»]
3H3VX-ray4.00L1-120[»]
3HOUX-ray3.20K/W1-120[»]
3HOVX-ray3.50K1-120[»]
3HOWX-ray3.60K1-120[»]
3HOXX-ray3.65K1-120[»]
3HOYX-ray3.40K1-120[»]
3HOZX-ray3.65K1-120[»]
3I4MX-ray3.70K1-120[»]
3I4NX-ray3.90K1-120[»]
3J1Nelectron microscopy16.00K1-120[»]
3K1FX-ray4.30K1-120[»]
3K7AX-ray3.80K1-120[»]
3M3YX-ray3.18K1-120[»]
3M4OX-ray3.57K1-120[»]
3PO2X-ray3.30K1-120[»]
3PO3X-ray3.30K1-120[»]
3QT1X-ray4.30K1-120[»]
3RZDX-ray3.30K1-120[»]
3RZOX-ray3.00K1-120[»]
3S14X-ray2.85K1-120[»]
3S15X-ray3.30K1-120[»]
3S16X-ray3.24K1-120[»]
3S17X-ray3.20K1-120[»]
3S1MX-ray3.13K1-120[»]
3S1NX-ray3.10K1-120[»]
3S1QX-ray3.30K1-120[»]
3S1RX-ray3.20K1-120[»]
3S2DX-ray3.20K1-120[»]
3S2HX-ray3.30K1-120[»]
4A3BX-ray3.50K1-120[»]
4A3CX-ray3.50K1-120[»]
4A3DX-ray3.40K1-120[»]
4A3EX-ray3.40K1-120[»]
4A3FX-ray3.50K1-120[»]
4A3GX-ray3.50K1-120[»]
4A3IX-ray3.80K1-120[»]
4A3JX-ray3.70K1-120[»]
4A3KX-ray3.50K1-120[»]
4A3LX-ray3.50K1-120[»]
4A3MX-ray3.90K1-120[»]
4A93X-ray3.40K1-120[»]
4BBRX-ray3.40K1-120[»]
4BBSX-ray3.60K1-120[»]
4BXXX-ray3.28K1-120[»]
4BXZX-ray4.80K1-120[»]
4BY1X-ray3.60K1-120[»]
4BY7X-ray3.15K1-120[»]
ProteinModelPortalP38902.
SMRP38902. Positions 1-114.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34399. 50 interactions.
DIPDIP-937N.
IntActP38902. 15 interactions.
MINTMINT-618340.
STRING4932.YOL005C.

Proteomic databases

PaxDbP38902.
PeptideAtlasP38902.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL005C; YOL005C; YOL005C.
GeneID854157.
KEGGsce:YOL005C.

Organism-specific databases

CYGDYOL005c.
SGDS000005365. RPB11.

Phylogenomic databases

eggNOGCOG1761.
GeneTreeENSGT00550000074975.
HOGENOMHOG000179862.
KOK03008.
OMAFAAYKVE.
OrthoDBEOG7T4MZH.

Enzyme and pathway databases

BioCycYEAST:G3O-33422-MONOMER.

Gene expression databases

GenevestigatorP38902.

Family and domain databases

InterProIPR009025. RBP11-like_dimer.
IPR008193. RNA_pol_Rpb11_13-16kDa_CS.
[Graphical view]
SUPFAMSSF55257. SSF55257. 1 hit.
PROSITEPS01154. RNA_POL_L_13KD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38902.
NextBio975923.
PROP38902.

Entry information

Entry nameRPB11_YEAST
AccessionPrimary (citable) accession number: P38902
Secondary accession number(s): D6W262
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references