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Protein

Flocculation protein FLO5

Gene

FLO5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.

GO - Molecular functioni

  • mannose binding Source: SGD

GO - Biological processi

  • cell-substrate adhesion Source: SGD
  • coflocculation Source: SGD
  • flocculation Source: SGD
  • flocculation via cell wall protein-carbohydrate interaction Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31236-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flocculation protein FLO5
Short name:
Flocculin-5
Gene namesi
Name:FLO5
Ordered Locus Names:YHR211W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR211W.
SGDiS000001254. FLO5.

Subcellular locationi

  • Secretedcell wall 1 Publication
  • Membrane Curated; Lipid-anchorGPI-anchor Curated

  • Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).By similarity

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • extracellular region Source: UniProtKB-KW
  • fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

Pathology & Biotechi

Biotechnological usei

For many industrial applications in which the yeast Saccharomyces cerevisiae is used, e.g. beer, wine and alcohol production, appropriate flocculation behavior is one of the most important characteristics of a good production strain. The ability of yeast cells to flocculate is of considerable importance, as it provides an effective, environment-friendly, simple and cost-free way to separate yeast cells from the fermentation product at the end of fermentation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000002127525 – 1052Flocculation protein FLO5Add BLAST1028
PropeptideiPRO_00000212761053 – 1075Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1
Glycosylationi187N-linked (GlcNAc...)Sequence analysis1
Glycosylationi203N-linked (GlcNAc...)Sequence analysis1
Glycosylationi262N-linked (GlcNAc...)Sequence analysis1
Glycosylationi663N-linked (GlcNAc...)Sequence analysis1
Glycosylationi749N-linked (GlcNAc...)Sequence analysis1
Lipidationi1052GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

Extensively O-glycosylated.Curated
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By similarity).By similarity

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Interactioni

Protein-protein interaction databases

BioGridi36645. 7 interactors.
DIPiDIP-4056N.
IntActiP38894. 3 interactors.
MINTiMINT-498170.

Structurei

Secondary structure

11075
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 45Combined sources10
Helixi53 – 55Combined sources3
Helixi57 – 61Combined sources5
Helixi63 – 66Combined sources4
Beta strandi69 – 76Combined sources8
Beta strandi81 – 83Combined sources3
Beta strandi86 – 89Combined sources4
Beta strandi92 – 95Combined sources4
Helixi98 – 100Combined sources3
Turni103 – 105Combined sources3
Turni108 – 110Combined sources3
Turni125 – 128Combined sources4
Beta strandi135 – 144Combined sources10
Beta strandi147 – 157Combined sources11
Beta strandi160 – 168Combined sources9
Turni169 – 171Combined sources3
Beta strandi189 – 192Combined sources4
Beta strandi196 – 198Combined sources3
Beta strandi205 – 210Combined sources6
Beta strandi215 – 224Combined sources10
Beta strandi229 – 231Combined sources3
Beta strandi233 – 236Combined sources4
Beta strandi242 – 246Combined sources5
Turni248 – 250Combined sources3
Beta strandi251 – 253Combined sources3
Beta strandi264 – 266Combined sources3
Turni268 – 270Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XJPX-ray0.95A23-271[»]
2XJQX-ray1.35A23-271[»]
2XJRX-ray1.25A23-271[»]
2XJSX-ray1.30A23-271[»]
2XJTX-ray1.20A23-271[»]
2XJUX-ray1.70A23-271[»]
2XJVX-ray1.74A23-271[»]
4AHWX-ray1.50A23-271[»]
4AHXX-ray1.60A23-271[»]
4AHYX-ray1.70A23-271[»]
4AHZX-ray1.90A23-271[»]
4AI0X-ray1.80A23-271[»]
4AI1X-ray1.80A23-271[»]
4AI2X-ray1.79A23-271[»]
4AI3X-ray1.90A23-271[»]
ProteinModelPortaliP38894.
SMRiP38894.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38894.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati278 – 3221-11 PublicationAdd BLAST45
Repeati323 – 3671-21 PublicationAdd BLAST45
Repeati368 – 4121-31 PublicationAdd BLAST45
Repeati413 – 4571-41 PublicationAdd BLAST45
Repeati458 – 5021-51 PublicationAdd BLAST45
Repeati503 – 5471-61 PublicationAdd BLAST45
Repeati548 – 5921-71 PublicationAdd BLAST45
Repeati593 – 6371-81 PublicationAdd BLAST45
Repeati667 – 6862-11 PublicationAdd BLAST20
Repeati687 – 7062-21 PublicationAdd BLAST20
Repeati775 – 8253-11 PublicationAdd BLAST51
Repeati847 – 8973-21 PublicationAdd BLAST51
Repeati898 – 9483-31 PublicationAdd BLAST51

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni197 – 240Sugar recognitionBy similarityAdd BLAST44
Regioni278 – 6378 X 45 AA approximate tandem repeats, Thr-richAdd BLAST360
Regioni667 – 7062 X 20 AA approximate tandem repeats, Ser-richAdd BLAST40
Regioni775 – 9483 X 51 AA approximate repeats, Ser/Thr-richAdd BLAST174

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells (By similarity).By similarity

Sequence similaritiesi

Belongs to the flocculin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00660000095872.
HOGENOMiHOG000112611.
InParanoidiP38894.
OMAiDISTTTH.
OrthoDBiEOG092C2UYM.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
InterProiIPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14_dom.
[Graphical view]
PfamiPF00624. Flocculin. 8 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIAHHCIFL VILAFLALIN VASGATEACL PAGQRKSGMN INFYQYSLKD
60 70 80 90 100
SSTYSNAAYM AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED
110 120 130 140 150
SYGNWGCKGM GACSNSQGIA YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG
160 170 180 190 200
SYTFSFATVD DSAILSVGGS IAFECCAQEQ PPITSTNFTI NGIKPWDGSL
210 220 230 240 250
PDNITGTVYM YAGYYYPLKV VYSNAVSWGT LPISVELPDG TTVSDNFEGY
260 270 280 290 300
VYSFDDDLSQ SNCTIPDPSI HTTSTITTTT EPWTGTFTST STEMTTITDT
310 320 330 340 350
NGQLTDETVI VIRTPTTAST ITTTTEPWTG TFTSTSTEMT TVTGTNGQPT
360 370 380 390 400
DETVIVIRTP TSEGLITTTT EPWTGTFTST STEMTTVTGT NGQPTDETVI
410 420 430 440 450
VIRTPTSEGL ITTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP
460 470 480 490 500
TSEGLITTTT EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL
510 520 530 540 550
ISTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP TSEGLITTTT
560 570 580 590 600
EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL ITRTTEPWTG
610 620 630 640 650
TFTSTSTEVT TITGTNGQPT DETVIVIRTP TTAISSSLSS SSGQITSSIT
660 670 680 690 700
SSRPIITPFY PSNGTSVISS SVISSSVTSS LVTSSSFISS SVISSSTTTS
710 720 730 740 750
TSIFSESSTS SVIPTSSSTS GSSESKTSSA SSSSSSSSIS SESPKSPTNS
760 770 780 790 800
SSSLPPVTSA TTGQETASSL PPATTTKTSE QTTLVTVTSC ESHVCTESIS
810 820 830 840 850
SAIVSTATVT VSGVTTEYTT WCPISTTETT KQTKGTTEQT KGTTEQTTET
860 870 880 890 900
TKQTTVVTIS SCESDICSKT ASPAIVSTST ATINGVTTEY TTWCPISTTE
910 920 930 940 950
SKQQTTLVTV TSCESGVCSE TTSPAIVSTA TATVNDVVTV YPTWRPQTTN
960 970 980 990 1000
EQSVSSKMNS ATSETTTNTG AAETKTAVTS SLSRFNHAET QTASATDVIG
1010 1020 1030 1040 1050
HSSSVVSVSE TGNTMSLTSS GLSTMSQQPR STPASSMVGS STASLEISTY
1060 1070
AGSANSLLAG SGLSVFIASL LLAII
Length:1,075
Mass (Da):111,982
Last modified:February 1, 1995 - v1
Checksum:iD151B370B60C8D9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00029 Genomic DNA. Translation: AAB69731.1.
BK006934 Genomic DNA. Translation: DAA06904.1.
PIRiS48992.
RefSeqiNP_012081.1. NM_001179342.1.

Genome annotation databases

EnsemblFungiiYHR211W; YHR211W; YHR211W.
GeneIDi856618.
KEGGisce:YHR211W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00029 Genomic DNA. Translation: AAB69731.1.
BK006934 Genomic DNA. Translation: DAA06904.1.
PIRiS48992.
RefSeqiNP_012081.1. NM_001179342.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XJPX-ray0.95A23-271[»]
2XJQX-ray1.35A23-271[»]
2XJRX-ray1.25A23-271[»]
2XJSX-ray1.30A23-271[»]
2XJTX-ray1.20A23-271[»]
2XJUX-ray1.70A23-271[»]
2XJVX-ray1.74A23-271[»]
4AHWX-ray1.50A23-271[»]
4AHXX-ray1.60A23-271[»]
4AHYX-ray1.70A23-271[»]
4AHZX-ray1.90A23-271[»]
4AI0X-ray1.80A23-271[»]
4AI1X-ray1.80A23-271[»]
4AI2X-ray1.79A23-271[»]
4AI3X-ray1.90A23-271[»]
ProteinModelPortaliP38894.
SMRiP38894.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36645. 7 interactors.
DIPiDIP-4056N.
IntActiP38894. 3 interactors.
MINTiMINT-498170.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR211W; YHR211W; YHR211W.
GeneIDi856618.
KEGGisce:YHR211W.

Organism-specific databases

EuPathDBiFungiDB:YHR211W.
SGDiS000001254. FLO5.

Phylogenomic databases

GeneTreeiENSGT00660000095872.
HOGENOMiHOG000112611.
InParanoidiP38894.
OMAiDISTTTH.
OrthoDBiEOG092C2UYM.

Enzyme and pathway databases

BioCyciYEAST:G3O-31236-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP38894.
PROiP38894.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
InterProiIPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14_dom.
[Graphical view]
PfamiPF00624. Flocculin. 8 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFLO5_YEAST
AccessioniPrimary (citable) accession number: P38894
Secondary accession number(s): D3DLG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.