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P38894

- FLO5_YEAST

UniProt

P38894 - FLO5_YEAST

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Protein

Flocculation protein FLO5

Gene
FLO5, YHR211W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.

GO - Molecular functioni

  1. mannose binding Source: SGD

GO - Biological processi

  1. cell-substrate adhesion Source: SGD
  2. flocculation Source: SGD
  3. flocculation via cell wall protein-carbohydrate interaction Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31236-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flocculation protein FLO5
Short name:
Flocculin-5
Gene namesi
Name:FLO5
Ordered Locus Names:YHR211W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR211w.
SGDiS000001254. FLO5.

Subcellular locationi

Secretedcell wall. Membrane; Lipid-anchorGPI-anchor Reviewed prediction
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP) By similarity.1 Publication

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

Pathology & Biotechi

Biotechnological usei

For many industrial applications in which the yeast Saccharomyces cerevisiae is used, e.g. beer, wine and alcohol production, appropriate flocculation behavior is one of the most important characteristics of a good production strain. The ability of yeast cells to flocculate is of considerable importance, as it provides an effective, environment-friendly, simple and cost-free way to separate yeast cells from the fermentation product at the end of fermentation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Chaini25 – 10521028Flocculation protein FLO5PRO_0000021275Add
BLAST
Propeptidei1053 – 107523Removed in mature form Reviewed predictionPRO_0000021276Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi135 – 1351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi187 – 1871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi203 – 2031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi262 – 2621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi663 – 6631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi749 – 7491N-linked (GlcNAc...) Reviewed prediction
Lipidationi1052 – 10521GPI-anchor amidated glycine Reviewed prediction

Post-translational modificationi

Extensively O-glycosylated Inferred.
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Gene expression databases

GenevestigatoriP38894.

Interactioni

Protein-protein interaction databases

BioGridi36645. 6 interactions.
DIPiDIP-4056N.
IntActiP38894. 3 interactions.
MINTiMINT-498170.
STRINGi4932.YHR211W.

Structurei

Secondary structure

1
1075
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 4510
Helixi53 – 553
Helixi57 – 615
Helixi63 – 664
Beta strandi69 – 768
Beta strandi81 – 833
Beta strandi86 – 894
Beta strandi92 – 954
Helixi98 – 1003
Turni103 – 1053
Turni108 – 1103
Turni125 – 1284
Beta strandi135 – 14410
Beta strandi147 – 15711
Beta strandi160 – 1689
Turni169 – 1713
Beta strandi189 – 1924
Beta strandi196 – 1983
Beta strandi205 – 2106
Beta strandi215 – 22410
Beta strandi229 – 2313
Beta strandi233 – 2364
Beta strandi242 – 2465
Turni248 – 2503
Beta strandi251 – 2533
Beta strandi264 – 2663
Turni268 – 2703

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XJPX-ray0.95A23-271[»]
2XJQX-ray1.35A23-271[»]
2XJRX-ray1.25A23-271[»]
2XJSX-ray1.30A23-271[»]
2XJTX-ray1.20A23-271[»]
2XJUX-ray1.70A23-271[»]
2XJVX-ray1.74A23-271[»]
4AHWX-ray1.50A23-271[»]
4AHXX-ray1.60A23-271[»]
4AHYX-ray1.70A23-271[»]
4AHZX-ray1.90A23-271[»]
4AI0X-ray1.80A23-271[»]
4AI1X-ray1.80A23-271[»]
4AI2X-ray1.79A23-271[»]
4AI3X-ray1.90A23-271[»]
ProteinModelPortaliP38894.
SMRiP38894. Positions 23-271.

Miscellaneous databases

EvolutionaryTraceiP38894.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati278 – 322451-1Add
BLAST
Repeati323 – 367451-2Add
BLAST
Repeati368 – 412451-3Add
BLAST
Repeati413 – 457451-4Add
BLAST
Repeati458 – 502451-5Add
BLAST
Repeati503 – 547451-6Add
BLAST
Repeati548 – 592451-7Add
BLAST
Repeati593 – 637451-8Add
BLAST
Repeati667 – 686202-1Add
BLAST
Repeati687 – 706202-2Add
BLAST
Repeati775 – 825513-1Add
BLAST
Repeati847 – 897513-2Add
BLAST
Repeati898 – 948513-3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 24044Sugar recognition By similarityAdd
BLAST
Regioni278 – 6373608 X 45 AA approximate tandem repeats, Thr-richAdd
BLAST
Regioni667 – 706402 X 20 AA approximate tandem repeats, Ser-richAdd
BLAST
Regioni775 – 9481743 X 51 AA approximate repeats, Ser/Thr-richAdd
BLAST

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells By similarity.

Sequence similaritiesi

Belongs to the flocculin family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00660000095872.
HOGENOMiHOG000112611.
OMAiISHTIRW.
OrthoDBiEOG7H4F6F.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
InterProiIPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view]
PfamiPF00624. Flocculin. 8 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38894-1 [UniParc]FASTAAdd to Basket

« Hide

MTIAHHCIFL VILAFLALIN VASGATEACL PAGQRKSGMN INFYQYSLKD     50
SSTYSNAAYM AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED 100
SYGNWGCKGM GACSNSQGIA YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG 150
SYTFSFATVD DSAILSVGGS IAFECCAQEQ PPITSTNFTI NGIKPWDGSL 200
PDNITGTVYM YAGYYYPLKV VYSNAVSWGT LPISVELPDG TTVSDNFEGY 250
VYSFDDDLSQ SNCTIPDPSI HTTSTITTTT EPWTGTFTST STEMTTITDT 300
NGQLTDETVI VIRTPTTAST ITTTTEPWTG TFTSTSTEMT TVTGTNGQPT 350
DETVIVIRTP TSEGLITTTT EPWTGTFTST STEMTTVTGT NGQPTDETVI 400
VIRTPTSEGL ITTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP 450
TSEGLITTTT EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL 500
ISTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP TSEGLITTTT 550
EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL ITRTTEPWTG 600
TFTSTSTEVT TITGTNGQPT DETVIVIRTP TTAISSSLSS SSGQITSSIT 650
SSRPIITPFY PSNGTSVISS SVISSSVTSS LVTSSSFISS SVISSSTTTS 700
TSIFSESSTS SVIPTSSSTS GSSESKTSSA SSSSSSSSIS SESPKSPTNS 750
SSSLPPVTSA TTGQETASSL PPATTTKTSE QTTLVTVTSC ESHVCTESIS 800
SAIVSTATVT VSGVTTEYTT WCPISTTETT KQTKGTTEQT KGTTEQTTET 850
TKQTTVVTIS SCESDICSKT ASPAIVSTST ATINGVTTEY TTWCPISTTE 900
SKQQTTLVTV TSCESGVCSE TTSPAIVSTA TATVNDVVTV YPTWRPQTTN 950
EQSVSSKMNS ATSETTTNTG AAETKTAVTS SLSRFNHAET QTASATDVIG 1000
HSSSVVSVSE TGNTMSLTSS GLSTMSQQPR STPASSMVGS STASLEISTY 1050
AGSANSLLAG SGLSVFIASL LLAII 1075
Length:1,075
Mass (Da):111,982
Last modified:February 1, 1995 - v1
Checksum:iD151B370B60C8D9F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00029 Genomic DNA. Translation: AAB69731.1.
BK006934 Genomic DNA. Translation: DAA06904.1.
PIRiS48992.
RefSeqiNP_012081.1. NM_001179342.1.

Genome annotation databases

EnsemblFungiiYHR211W; YHR211W; YHR211W.
GeneIDi856618.
KEGGisce:YHR211W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00029 Genomic DNA. Translation: AAB69731.1 .
BK006934 Genomic DNA. Translation: DAA06904.1 .
PIRi S48992.
RefSeqi NP_012081.1. NM_001179342.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2XJP X-ray 0.95 A 23-271 [» ]
2XJQ X-ray 1.35 A 23-271 [» ]
2XJR X-ray 1.25 A 23-271 [» ]
2XJS X-ray 1.30 A 23-271 [» ]
2XJT X-ray 1.20 A 23-271 [» ]
2XJU X-ray 1.70 A 23-271 [» ]
2XJV X-ray 1.74 A 23-271 [» ]
4AHW X-ray 1.50 A 23-271 [» ]
4AHX X-ray 1.60 A 23-271 [» ]
4AHY X-ray 1.70 A 23-271 [» ]
4AHZ X-ray 1.90 A 23-271 [» ]
4AI0 X-ray 1.80 A 23-271 [» ]
4AI1 X-ray 1.80 A 23-271 [» ]
4AI2 X-ray 1.79 A 23-271 [» ]
4AI3 X-ray 1.90 A 23-271 [» ]
ProteinModelPortali P38894.
SMRi P38894. Positions 23-271.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36645. 6 interactions.
DIPi DIP-4056N.
IntActi P38894. 3 interactions.
MINTi MINT-498170.
STRINGi 4932.YHR211W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR211W ; YHR211W ; YHR211W .
GeneIDi 856618.
KEGGi sce:YHR211W.

Organism-specific databases

CYGDi YHR211w.
SGDi S000001254. FLO5.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00660000095872.
HOGENOMi HOG000112611.
OMAi ISHTIRW.
OrthoDBi EOG7H4F6F.

Enzyme and pathway databases

BioCyci YEAST:G3O-31236-MONOMER.

Miscellaneous databases

EvolutionaryTracei P38894.
NextBioi 982550.

Gene expression databases

Genevestigatori P38894.

Family and domain databases

Gene3Di 3.90.182.10. 1 hit.
InterProi IPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view ]
Pfami PF00624. Flocculin. 8 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view ]
SMARTi SM00758. PA14. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Localization of the dominant flocculation genes FLO5 and FLO8 of Saccharomyces cerevisiae."
    Teunissen A.W.R.H., van den Berg J.A., Steensma H.Y.
    Yeast 11:735-745(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE MAPPING.
  4. "Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family."
    Teunissen A.W.R.H., Steensma H.Y.
    Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  5. "Distribution of the flocculation protein, flop, at the cell surface during yeast growth: the availability of flop determines the flocculation level."
    Bony M., Barre P., Blondin B.
    Yeast 14:25-35(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. Cited for: BIOTECHNOLOGY.
  7. "Intragenic tandem repeats generate functional variability."
    Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
    Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPEATS.
  8. "Control by sugar of Saccharomyces cerevisiae flocculation for industrial ethanol production."
    Cunha A.F., Missawa S.K., Gomes L.H., Reis S.F., Pereira G.A.G.
    FEMS Yeast Res. 6:280-287(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTECHNOLOGY.

Entry informationi

Entry nameiFLO5_YEAST
AccessioniPrimary (citable) accession number: P38894
Secondary accession number(s): D3DLG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

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