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P38894

- FLO5_YEAST

UniProt

P38894 - FLO5_YEAST

Protein

Flocculation protein FLO5

Gene

FLO5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.

    GO - Molecular functioni

    1. mannose binding Source: SGD

    GO - Biological processi

    1. cell-substrate adhesion Source: SGD
    2. flocculation Source: SGD
    3. flocculation via cell wall protein-carbohydrate interaction Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31236-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Flocculation protein FLO5
    Short name:
    Flocculin-5
    Gene namesi
    Name:FLO5
    Ordered Locus Names:YHR211W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR211w.
    SGDiS000001254. FLO5.

    Subcellular locationi

    Secretedcell wall 1 Publication. Membrane Curated; Lipid-anchorGPI-anchor Curated
    Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).By similarity

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: UniProtKB-KW
    3. fungal-type cell wall Source: SGD

    Keywords - Cellular componenti

    Cell wall, Membrane, Secreted

    Pathology & Biotechi

    Biotechnological usei

    For many industrial applications in which the yeast Saccharomyces cerevisiae is used, e.g. beer, wine and alcohol production, appropriate flocculation behavior is one of the most important characteristics of a good production strain. The ability of yeast cells to flocculate is of considerable importance, as it provides an effective, environment-friendly, simple and cost-free way to separate yeast cells from the fermentation product at the end of fermentation.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 10521028Flocculation protein FLO5PRO_0000021275Add
    BLAST
    Propeptidei1053 – 107523Removed in mature formSequence AnalysisPRO_0000021276Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi663 – 6631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi749 – 7491N-linked (GlcNAc...)Sequence Analysis
    Lipidationi1052 – 10521GPI-anchor amidated glycineSequence Analysis

    Post-translational modificationi

    Extensively O-glycosylated.Curated
    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.By similarity

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP38894.

    Interactioni

    Protein-protein interaction databases

    BioGridi36645. 6 interactions.
    DIPiDIP-4056N.
    IntActiP38894. 3 interactions.
    MINTiMINT-498170.
    STRINGi4932.YHR211W.

    Structurei

    Secondary structure

    1
    1075
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi36 – 4510
    Helixi53 – 553
    Helixi57 – 615
    Helixi63 – 664
    Beta strandi69 – 768
    Beta strandi81 – 833
    Beta strandi86 – 894
    Beta strandi92 – 954
    Helixi98 – 1003
    Turni103 – 1053
    Turni108 – 1103
    Turni125 – 1284
    Beta strandi135 – 14410
    Beta strandi147 – 15711
    Beta strandi160 – 1689
    Turni169 – 1713
    Beta strandi189 – 1924
    Beta strandi196 – 1983
    Beta strandi205 – 2106
    Beta strandi215 – 22410
    Beta strandi229 – 2313
    Beta strandi233 – 2364
    Beta strandi242 – 2465
    Turni248 – 2503
    Beta strandi251 – 2533
    Beta strandi264 – 2663
    Turni268 – 2703

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XJPX-ray0.95A23-271[»]
    2XJQX-ray1.35A23-271[»]
    2XJRX-ray1.25A23-271[»]
    2XJSX-ray1.30A23-271[»]
    2XJTX-ray1.20A23-271[»]
    2XJUX-ray1.70A23-271[»]
    2XJVX-ray1.74A23-271[»]
    4AHWX-ray1.50A23-271[»]
    4AHXX-ray1.60A23-271[»]
    4AHYX-ray1.70A23-271[»]
    4AHZX-ray1.90A23-271[»]
    4AI0X-ray1.80A23-271[»]
    4AI1X-ray1.80A23-271[»]
    4AI2X-ray1.79A23-271[»]
    4AI3X-ray1.90A23-271[»]
    ProteinModelPortaliP38894.
    SMRiP38894. Positions 23-271.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38894.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati278 – 322451-11 PublicationAdd
    BLAST
    Repeati323 – 367451-21 PublicationAdd
    BLAST
    Repeati368 – 412451-31 PublicationAdd
    BLAST
    Repeati413 – 457451-41 PublicationAdd
    BLAST
    Repeati458 – 502451-51 PublicationAdd
    BLAST
    Repeati503 – 547451-61 PublicationAdd
    BLAST
    Repeati548 – 592451-71 PublicationAdd
    BLAST
    Repeati593 – 637451-81 PublicationAdd
    BLAST
    Repeati667 – 686202-11 PublicationAdd
    BLAST
    Repeati687 – 706202-21 PublicationAdd
    BLAST
    Repeati775 – 825513-11 PublicationAdd
    BLAST
    Repeati847 – 897513-21 PublicationAdd
    BLAST
    Repeati898 – 948513-31 PublicationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni197 – 24044Sugar recognitionBy similarityAdd
    BLAST
    Regioni278 – 6373608 X 45 AA approximate tandem repeats, Thr-richAdd
    BLAST
    Regioni667 – 706402 X 20 AA approximate tandem repeats, Ser-richAdd
    BLAST
    Regioni775 – 9481743 X 51 AA approximate repeats, Ser/Thr-richAdd
    BLAST

    Domaini

    The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells By similarity.By similarity

    Sequence similaritiesi

    Belongs to the flocculin family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00660000095872.
    HOGENOMiHOG000112611.
    OMAiISHTIRW.
    OrthoDBiEOG7H4F6F.

    Family and domain databases

    Gene3Di3.90.182.10. 1 hit.
    InterProiIPR001389. Flocculin.
    IPR025928. Flocculin_t3_rpt.
    IPR011658. PA14.
    [Graphical view]
    PfamiPF00624. Flocculin. 8 hits.
    PF13928. Flocculin_t3. 3 hits.
    PF07691. PA14. 1 hit.
    [Graphical view]
    SMARTiSM00758. PA14. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38894-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTIAHHCIFL VILAFLALIN VASGATEACL PAGQRKSGMN INFYQYSLKD     50
    SSTYSNAAYM AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED 100
    SYGNWGCKGM GACSNSQGIA YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG 150
    SYTFSFATVD DSAILSVGGS IAFECCAQEQ PPITSTNFTI NGIKPWDGSL 200
    PDNITGTVYM YAGYYYPLKV VYSNAVSWGT LPISVELPDG TTVSDNFEGY 250
    VYSFDDDLSQ SNCTIPDPSI HTTSTITTTT EPWTGTFTST STEMTTITDT 300
    NGQLTDETVI VIRTPTTAST ITTTTEPWTG TFTSTSTEMT TVTGTNGQPT 350
    DETVIVIRTP TSEGLITTTT EPWTGTFTST STEMTTVTGT NGQPTDETVI 400
    VIRTPTSEGL ITTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP 450
    TSEGLITTTT EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL 500
    ISTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP TSEGLITTTT 550
    EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL ITRTTEPWTG 600
    TFTSTSTEVT TITGTNGQPT DETVIVIRTP TTAISSSLSS SSGQITSSIT 650
    SSRPIITPFY PSNGTSVISS SVISSSVTSS LVTSSSFISS SVISSSTTTS 700
    TSIFSESSTS SVIPTSSSTS GSSESKTSSA SSSSSSSSIS SESPKSPTNS 750
    SSSLPPVTSA TTGQETASSL PPATTTKTSE QTTLVTVTSC ESHVCTESIS 800
    SAIVSTATVT VSGVTTEYTT WCPISTTETT KQTKGTTEQT KGTTEQTTET 850
    TKQTTVVTIS SCESDICSKT ASPAIVSTST ATINGVTTEY TTWCPISTTE 900
    SKQQTTLVTV TSCESGVCSE TTSPAIVSTA TATVNDVVTV YPTWRPQTTN 950
    EQSVSSKMNS ATSETTTNTG AAETKTAVTS SLSRFNHAET QTASATDVIG 1000
    HSSSVVSVSE TGNTMSLTSS GLSTMSQQPR STPASSMVGS STASLEISTY 1050
    AGSANSLLAG SGLSVFIASL LLAII 1075
    Length:1,075
    Mass (Da):111,982
    Last modified:February 1, 1995 - v1
    Checksum:iD151B370B60C8D9F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00029 Genomic DNA. Translation: AAB69731.1.
    BK006934 Genomic DNA. Translation: DAA06904.1.
    PIRiS48992.
    RefSeqiNP_012081.1. NM_001179342.1.

    Genome annotation databases

    EnsemblFungiiYHR211W; YHR211W; YHR211W.
    GeneIDi856618.
    KEGGisce:YHR211W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00029 Genomic DNA. Translation: AAB69731.1 .
    BK006934 Genomic DNA. Translation: DAA06904.1 .
    PIRi S48992.
    RefSeqi NP_012081.1. NM_001179342.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2XJP X-ray 0.95 A 23-271 [» ]
    2XJQ X-ray 1.35 A 23-271 [» ]
    2XJR X-ray 1.25 A 23-271 [» ]
    2XJS X-ray 1.30 A 23-271 [» ]
    2XJT X-ray 1.20 A 23-271 [» ]
    2XJU X-ray 1.70 A 23-271 [» ]
    2XJV X-ray 1.74 A 23-271 [» ]
    4AHW X-ray 1.50 A 23-271 [» ]
    4AHX X-ray 1.60 A 23-271 [» ]
    4AHY X-ray 1.70 A 23-271 [» ]
    4AHZ X-ray 1.90 A 23-271 [» ]
    4AI0 X-ray 1.80 A 23-271 [» ]
    4AI1 X-ray 1.80 A 23-271 [» ]
    4AI2 X-ray 1.79 A 23-271 [» ]
    4AI3 X-ray 1.90 A 23-271 [» ]
    ProteinModelPortali P38894.
    SMRi P38894. Positions 23-271.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36645. 6 interactions.
    DIPi DIP-4056N.
    IntActi P38894. 3 interactions.
    MINTi MINT-498170.
    STRINGi 4932.YHR211W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR211W ; YHR211W ; YHR211W .
    GeneIDi 856618.
    KEGGi sce:YHR211W.

    Organism-specific databases

    CYGDi YHR211w.
    SGDi S000001254. FLO5.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00660000095872.
    HOGENOMi HOG000112611.
    OMAi ISHTIRW.
    OrthoDBi EOG7H4F6F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31236-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P38894.
    NextBioi 982550.

    Gene expression databases

    Genevestigatori P38894.

    Family and domain databases

    Gene3Di 3.90.182.10. 1 hit.
    InterProi IPR001389. Flocculin.
    IPR025928. Flocculin_t3_rpt.
    IPR011658. PA14.
    [Graphical view ]
    Pfami PF00624. Flocculin. 8 hits.
    PF13928. Flocculin_t3. 3 hits.
    PF07691. PA14. 1 hit.
    [Graphical view ]
    SMARTi SM00758. PA14. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Localization of the dominant flocculation genes FLO5 and FLO8 of Saccharomyces cerevisiae."
      Teunissen A.W.R.H., van den Berg J.A., Steensma H.Y.
      Yeast 11:735-745(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE MAPPING.
    4. "Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family."
      Teunissen A.W.R.H., Steensma H.Y.
      Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    5. "Distribution of the flocculation protein, flop, at the cell surface during yeast growth: the availability of flop determines the flocculation level."
      Bony M., Barre P., Blondin B.
      Yeast 14:25-35(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. Cited for: BIOTECHNOLOGY.
    7. "Intragenic tandem repeats generate functional variability."
      Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
      Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REPEATS.
    8. "Control by sugar of Saccharomyces cerevisiae flocculation for industrial ethanol production."
      Cunha A.F., Missawa S.K., Gomes L.H., Reis S.F., Pereira G.A.G.
      FEMS Yeast Res. 6:280-287(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOTECHNOLOGY.

    Entry informationi

    Entry nameiFLO5_YEAST
    AccessioniPrimary (citable) accession number: P38894
    Secondary accession number(s): D3DLG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3