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P38894 (FLO5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Flocculation protein FLO5

Short name=Flocculin-5
Gene names
Name:FLO5
Ordered Locus Names:YHR211W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1075 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.

Subcellular location

Secretedcell wall. Membrane; Lipid-anchorGPI-anchor Potential. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP) By similarity. Ref.5

Domain

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells By similarity.

Post-translational modification

Extensively O-glycosylated Probable.

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.

Biotechnological use

For many industrial applications in which the yeast Saccharomyces cerevisiae is used, e.g. beer, wine and alcohol production, appropriate flocculation behavior is one of the most important characteristics of a good production strain. The ability of yeast cells to flocculate is of considerable importance, as it provides an effective, environment-friendly, simple and cost-free way to separate yeast cells from the fermentation product at the end of fermentation. Ref.6 Ref.8

Sequence similarities

Belongs to the flocculin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 10521028Flocculation protein FLO5
PRO_0000021275
Propeptide1053 – 107523Removed in mature form Potential
PRO_0000021276

Regions

Repeat278 – 322451-1
Repeat323 – 367451-2
Repeat368 – 412451-3
Repeat413 – 457451-4
Repeat458 – 502451-5
Repeat503 – 547451-6
Repeat548 – 592451-7
Repeat593 – 637451-8
Repeat667 – 686202-1
Repeat687 – 706202-2
Repeat775 – 825513-1
Repeat847 – 897513-2
Repeat898 – 948513-3
Region197 – 24044Sugar recognition By similarity
Region278 – 6373608 X 45 AA approximate tandem repeats, Thr-rich
Region667 – 706402 X 20 AA approximate tandem repeats, Ser-rich
Region775 – 9481743 X 51 AA approximate repeats, Ser/Thr-rich

Amino acid modifications

Lipidation10521GPI-anchor amidated glycine Potential
Glycosylation1351N-linked (GlcNAc...) Potential
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation2031N-linked (GlcNAc...) Potential
Glycosylation2621N-linked (GlcNAc...) Potential
Glycosylation6631N-linked (GlcNAc...) Potential
Glycosylation7491N-linked (GlcNAc...) Potential

Secondary structure

..................................................... 1075
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38894 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: D151B370B60C8D9F

FASTA1,075111,982
        10         20         30         40         50         60 
MTIAHHCIFL VILAFLALIN VASGATEACL PAGQRKSGMN INFYQYSLKD SSTYSNAAYM 

        70         80         90        100        110        120 
AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED SYGNWGCKGM GACSNSQGIA 

       130        140        150        160        170        180 
YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG SYTFSFATVD DSAILSVGGS IAFECCAQEQ 

       190        200        210        220        230        240 
PPITSTNFTI NGIKPWDGSL PDNITGTVYM YAGYYYPLKV VYSNAVSWGT LPISVELPDG 

       250        260        270        280        290        300 
TTVSDNFEGY VYSFDDDLSQ SNCTIPDPSI HTTSTITTTT EPWTGTFTST STEMTTITDT 

       310        320        330        340        350        360 
NGQLTDETVI VIRTPTTAST ITTTTEPWTG TFTSTSTEMT TVTGTNGQPT DETVIVIRTP 

       370        380        390        400        410        420 
TSEGLITTTT EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL ITTTTEPWTG 

       430        440        450        460        470        480 
TFTSTSTEVT TITGTNGQPT DETVIVIRTP TSEGLITTTT EPWTGTFTST STEMTTVTGT 

       490        500        510        520        530        540 
NGQPTDETVI VIRTPTSEGL ISTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP 

       550        560        570        580        590        600 
TSEGLITTTT EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL ITRTTEPWTG 

       610        620        630        640        650        660 
TFTSTSTEVT TITGTNGQPT DETVIVIRTP TTAISSSLSS SSGQITSSIT SSRPIITPFY 

       670        680        690        700        710        720 
PSNGTSVISS SVISSSVTSS LVTSSSFISS SVISSSTTTS TSIFSESSTS SVIPTSSSTS 

       730        740        750        760        770        780 
GSSESKTSSA SSSSSSSSIS SESPKSPTNS SSSLPPVTSA TTGQETASSL PPATTTKTSE 

       790        800        810        820        830        840 
QTTLVTVTSC ESHVCTESIS SAIVSTATVT VSGVTTEYTT WCPISTTETT KQTKGTTEQT 

       850        860        870        880        890        900 
KGTTEQTTET TKQTTVVTIS SCESDICSKT ASPAIVSTST ATINGVTTEY TTWCPISTTE 

       910        920        930        940        950        960 
SKQQTTLVTV TSCESGVCSE TTSPAIVSTA TATVNDVVTV YPTWRPQTTN EQSVSSKMNS 

       970        980        990       1000       1010       1020 
ATSETTTNTG AAETKTAVTS SLSRFNHAET QTASATDVIG HSSSVVSVSE TGNTMSLTSS 

      1030       1040       1050       1060       1070 
GLSTMSQQPR STPASSMVGS STASLEISTY AGSANSLLAG SGLSVFIASL LLAII 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Localization of the dominant flocculation genes FLO5 and FLO8 of Saccharomyces cerevisiae."
Teunissen A.W.R.H., van den Berg J.A., Steensma H.Y.
Yeast 11:735-745(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE MAPPING.
[4]"Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family."
Teunissen A.W.R.H., Steensma H.Y.
Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[5]"Distribution of the flocculation protein, flop, at the cell surface during yeast growth: the availability of flop determines the flocculation level."
Bony M., Barre P., Blondin B.
Yeast 14:25-35(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Yeast flocculation: what brewers should know."
Verstrepen K.J., Derdelinckx G., Verachtert H., Delvaux F.R.
Appl. Microbiol. Biotechnol. 61:197-205(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTECHNOLOGY.
[7]"Intragenic tandem repeats generate functional variability."
Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REPEATS.
[8]"Control by sugar of Saccharomyces cerevisiae flocculation for industrial ethanol production."
Cunha A.F., Missawa S.K., Gomes L.H., Reis S.F., Pereira G.A.G.
FEMS Yeast Res. 6:280-287(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTECHNOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00029 Genomic DNA. Translation: AAB69731.1.
BK006934 Genomic DNA. Translation: DAA06904.1.
PIRS48992.
RefSeqNP_012081.1. NM_001179342.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2XJPX-ray0.95A23-271[»]
2XJQX-ray1.35A23-271[»]
2XJRX-ray1.25A23-271[»]
2XJSX-ray1.30A23-271[»]
2XJTX-ray1.20A23-271[»]
2XJUX-ray1.70A23-271[»]
2XJVX-ray1.74A23-271[»]
4AHWX-ray1.50A23-271[»]
4AHXX-ray1.60A23-271[»]
4AHYX-ray1.70A23-271[»]
4AHZX-ray1.90A23-271[»]
4AI0X-ray1.80A23-271[»]
4AI1X-ray1.80A23-271[»]
4AI2X-ray1.79A23-271[»]
4AI3X-ray1.90A23-271[»]
ProteinModelPortalP38894.
SMRP38894. Positions 23-271.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36645. 6 interactions.
DIPDIP-4056N.
IntActP38894. 3 interactions.
MINTMINT-498170.
STRING4932.YHR211W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR211W; YHR211W; YHR211W.
GeneID856618.
KEGGsce:YHR211W.

Organism-specific databases

CYGDYHR211w.
SGDS000001254. FLO5.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00660000095872.
HOGENOMHOG000112611.
OMADISTTTH.
OrthoDBEOG7H4F6F.

Enzyme and pathway databases

BioCycYEAST:G3O-31236-MONOMER.

Gene expression databases

GenevestigatorP38894.

Family and domain databases

Gene3D3.90.182.10. 1 hit.
InterProIPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view]
PfamPF00624. Flocculin. 8 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTSM00758. PA14. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38894.
NextBio982550.

Entry information

Entry nameFLO5_YEAST
AccessionPrimary (citable) accession number: P38894
Secondary accession number(s): D3DLG0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references