P38894 (FLO5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flocculation protein FLO5
Short name=Flocculin-5

Gene names

Name:	FLO5
Ordered Locus Names:	YHR211W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1075 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.
Subcellular location	Secreted › cell wall. Membrane; Lipid-anchor › GPI-anchor Potential. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP) By similarity. Ref.5
Domain	The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells By similarity.
Post-translational modification	Extensively O-glycosylated Probable. The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.
Biotechnological use	For many industrial applications in which the yeast Saccharomyces cerevisiae is used, e.g. beer, wine and alcohol production, appropriate flocculation behavior is one of the most important characteristics of a good production strain. The ability of yeast cells to flocculate is of considerable importance, as it provides an effective, environment-friendly, simple and cost-free way to separate yeast cells from the fermentation product at the end of fermentation. Ref.6 Ref.8
Sequence similarities	Belongs to the flocculin family.

Ontologies

Keywords
Cellular component	Cell wall Membrane Secreted
Domain	Repeat Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-substrate adhesion Inferred from mutant phenotype PubMed 19087208. Source: SGD flocculation via cell wall protein-carbohydrate interaction Inferred from mutant phenotype PubMed 19087208. Source: SGD
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall Inferred from direct assay Ref.5. Source: SGD
Molecular_function	mannose binding Inferred from mutant phenotype PubMed 2665372. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Potential

Chain

25 – 1052

1028

Flocculation protein FLO5

PRO_0000021275

Propeptide

1053 – 1075

23

Removed in mature form Potential

PRO_0000021276

Regions

Repeat

278 – 322

45

1-1

Repeat

323 – 367

45

1-2

Repeat

368 – 412

45

1-3

Repeat

413 – 457

45

1-4

Repeat

458 – 502

45

1-5

Repeat

503 – 547

45

1-6

Repeat

548 – 592

45

1-7

Repeat

593 – 637

45

1-8

Repeat

667 – 686

20

2-1

Repeat

687 – 706

20

2-2

Repeat

775 – 825

51

3-1

Repeat

847 – 897

51

3-2

Repeat

898 – 948

51

3-3

Region

197 – 240

44

Sugar recognition By similarity

Region

278 – 637

360

8 X 45 AA approximate tandem repeats, Thr-rich

Region

667 – 706

40

2 X 20 AA approximate tandem repeats, Ser-rich

Region

775 – 948

174

3 X 51 AA approximate repeats, Ser/Thr-rich

Amino acid modifications

Lipidation

1052

1

GPI-anchor amidated glycine Potential

Glycosylation

135

1

N-linked (GlcNAc...) Potential

Glycosylation

187

1

N-linked (GlcNAc...) Potential

Glycosylation

203

1

N-linked (GlcNAc...) Potential

Glycosylation

262

1

N-linked (GlcNAc...) Potential

Glycosylation

663

1

N-linked (GlcNAc...) Potential

Glycosylation

749

1

N-linked (GlcNAc...) Potential

Secondary structure

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1075

Helix Strand Turn

Details...

Beta strand

36 – 45

10

Helix

53 – 55

3

Helix

57 – 61

5

Helix

63 – 66

4

Beta strand

69 – 76

8

Beta strand

81 – 83

3

Beta strand

86 – 89

4

Beta strand

92 – 95

4

Helix

98 – 100

3

Turn

103 – 105

3

Turn

108 – 110

3

Turn

125 – 128

4

Beta strand

135 – 144

10

Beta strand

147 – 157

11

Beta strand

160 – 168

9

Turn

169 – 171

3

Beta strand

189 – 192

4

Beta strand

196 – 198

3

Beta strand

205 – 210

6

Beta strand

215 – 224

10

Beta strand

229 – 231

3

Beta strand

233 – 236

4

Beta strand

242 – 246

5

Turn

248 – 250

3

Beta strand

251 – 253

3

Beta strand

264 – 266

3

Turn

268 – 270

3

Sequences

	Sequence		Length	Mass (Da)
	P38894 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D151B370B60C8D9F	FASTA	1,075	111,982
	10 20 30 40 50 60 MTIAHHCIFL VILAFLALIN VASGATEACL PAGQRKSGMN INFYQYSLKD SSTYSNAAYM 70 80 90 100 110 120 AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED SYGNWGCKGM GACSNSQGIA 130 140 150 160 170 180 YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG SYTFSFATVD DSAILSVGGS IAFECCAQEQ 190 200 210 220 230 240 PPITSTNFTI NGIKPWDGSL PDNITGTVYM YAGYYYPLKV VYSNAVSWGT LPISVELPDG 250 260 270 280 290 300 TTVSDNFEGY VYSFDDDLSQ SNCTIPDPSI HTTSTITTTT EPWTGTFTST STEMTTITDT 310 320 330 340 350 360 NGQLTDETVI VIRTPTTAST ITTTTEPWTG TFTSTSTEMT TVTGTNGQPT DETVIVIRTP 370 380 390 400 410 420 TSEGLITTTT EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL ITTTTEPWTG 430 440 450 460 470 480 TFTSTSTEVT TITGTNGQPT DETVIVIRTP TSEGLITTTT EPWTGTFTST STEMTTVTGT 490 500 510 520 530 540 NGQPTDETVI VIRTPTSEGL ISTTTEPWTG TFTSTSTEVT TITGTNGQPT DETVIVIRTP 550 560 570 580 590 600 TSEGLITTTT EPWTGTFTST STEMTTVTGT NGQPTDETVI VIRTPTSEGL ITRTTEPWTG 610 620 630 640 650 660 TFTSTSTEVT TITGTNGQPT DETVIVIRTP TTAISSSLSS SSGQITSSIT SSRPIITPFY 670 680 690 700 710 720 PSNGTSVISS SVISSSVTSS LVTSSSFISS SVISSSTTTS TSIFSESSTS SVIPTSSSTS 730 740 750 760 770 780 GSSESKTSSA SSSSSSSSIS SESPKSPTNS SSSLPPVTSA TTGQETASSL PPATTTKTSE 790 800 810 820 830 840 QTTLVTVTSC ESHVCTESIS SAIVSTATVT VSGVTTEYTT WCPISTTETT KQTKGTTEQT 850 860 870 880 890 900 KGTTEQTTET TKQTTVVTIS SCESDICSKT ASPAIVSTST ATINGVTTEY TTWCPISTTE 910 920 930 940 950 960 SKQQTTLVTV TSCESGVCSE TTSPAIVSTA TATVNDVVTV YPTWRPQTTN EQSVSSKMNS 970 980 990 1000 1010 1020 ATSETTTNTG AAETKTAVTS SLSRFNHAET QTASATDVIG HSSSVVSVSE TGNTMSLTSS 1030 1040 1050 1060 1070 GLSTMSQQPR STPASSMVGS STASLEISTY AGSANSLLAG SGLSVFIASL LLAII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Localization of the dominant flocculation genes FLO5 and FLO8 of Saccharomyces cerevisiae." Teunissen A.W.R.H., van den Berg J.A., Steensma H.Y. Yeast 11:735-745(1995) [PubMed] [Europe PMC] [Abstract] Cited for: GENE MAPPING.
[4]	"Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family." Teunissen A.W.R.H., Steensma H.Y. Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[5]	"Distribution of the flocculation protein, flop, at the cell surface during yeast growth: the availability of flop determines the flocculation level." Bony M., Barre P., Blondin B. Yeast 14:25-35(1998) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Yeast flocculation: what brewers should know." Verstrepen K.J., Derdelinckx G., Verachtert H., Delvaux F.R. Appl. Microbiol. Biotechnol. 61:197-205(2003) [PubMed] [Europe PMC] [Abstract] Cited for: BIOTECHNOLOGY.
[7]	"Intragenic tandem repeats generate functional variability." Verstrepen K.J., Jansen A., Lewitter F., Fink G.R. Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract] Cited for: REPEATS.
[8]	"Control by sugar of Saccharomyces cerevisiae flocculation for industrial ethanol production." Cunha A.F., Missawa S.K., Gomes L.H., Reis S.F., Pereira G.A.G. FEMS Yeast Res. 6:280-287(2006) [PubMed] [Europe PMC] [Abstract] Cited for: BIOTECHNOLOGY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00029 Genomic DNA. Translation: AAB69731.1.
BK006934 Genomic DNA. Translation: DAA06904.1.

PIR

S48992.

RefSeq

NP_012081.1. NM_001179342.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XJP	X-ray	0.95	A	23-271	[»]
2XJQ	X-ray	1.35	A	23-271	[»]
2XJR	X-ray	1.25	A	23-271	[»]
2XJS	X-ray	1.30	A	23-271	[»]
2XJT	X-ray	1.20	A	23-271	[»]
2XJU	X-ray	1.70	A	23-271	[»]
2XJV	X-ray	1.74	A	23-271	[»]
4AHW	X-ray	1.50	A	23-271	[»]
4AHX	X-ray	1.60	A	23-271	[»]
4AHY	X-ray	1.70	A	23-271	[»]
4AHZ	X-ray	1.90	A	23-271	[»]
4AI0	X-ray	1.80	A	23-271	[»]
4AI1	X-ray	1.80	A	23-271	[»]
4AI2	X-ray	1.79	A	23-271	[»]
4AI3	X-ray	1.90	A	23-271	[»]

ProteinModelPortal

P38894.

SMR

P38894. Positions 23-271.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4056N.

IntAct

P38894. 3 interactions.

MINT

MINT-498170.

STRING

4932.YHR211W.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YHR211W; YHR211W; YHR211W.

GeneID

856618.

KEGG

sce:YHR211W.

Organism-specific databases

CYGD

YHR211w.

SGD

S000001254. FLO5.

Phylogenomic databases

eggNOG

NOG12793.

GeneTree

ENSGT00660000095872.

HOGENOM

HOG000112611.

OMA

DISTTTH.

OrthoDB

EOG43XZD2.

Gene expression databases

Genevestigator

P38894.

GermOnline

YHR211W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view]

Pfam

PF00624. Flocculin. 8 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view]

SMART

SM00758. PA14. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P38894.

NextBio

982550.

Entry information

Entry name

FLO5_YEAST

Accession

Primary (citable) accession number: P38894
Secondary accession number(s): D3DLG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program